Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004616

Physical Location & Seq

Genomic Coordinates Forward (+)

17799961 .. 17805034

Gene Structure 5074 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1087600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3438 bp

ATGGAGGACAGAAGTGAAGACGACGACAGGGACGCATTGGCGGGGCTAAGCAACGCCCCTCCACCCCGCAAGTCACATTCATTAAGCCAGCAGCTCCGTGCAACCTCCGCCCAAAAACGCCACCACCAATTACGAAAGCATAGCCTCGACGACGTCAGCGTCTTCCCCAAGAGCGTTCATAACAATAATAATGTCGACTACTACGACTCTTCTGACGACGATTTCTTTCCTTACTCCACCTCTAACAGCACCATCACCACGACAACCACTAGTAATGCTGTTGGCGTTGGTGATCACGACGTGTACGTGGGCACCACTCACTCCCAGAGGCTGGACCAAAGCCTCTGCATGGAGGCTGGTGATGATCCTGATGGCAACAGGGAATGCCAGCCGTTGGCCGAATTTATTGGCAGCGGCGGTGGCACTGGCATCTTCAAGGTTCCAACTCGCGCATCCGTCCATCCTGGTCGTCCCCCCTGCTTGGAGCTGAGACCCCACCCTCTCAGGGAGACCCAGGTCGGCAGGTTCCTCAGGACCATTGCCTGCACCAACACCCAGCTGTGGGCTGGCCAGGAGGGCGGAGTCAGGGTCTGGAATTTGAAGGGCGTCTTTGAACCTGGTTTTGGTATTGGTGGCAGGGTCATGAGGGGTGATGAGGATGCCGCCCCATACTATGAATCCGCCAACACCTCTCCCACCCATTGCCTCATGGTTGATACCGGCAATCGCTTGATTTGGACTGGCCATAAGGATGGTAAGATCAGGTCTTGGAAGATGGATCAGTCTTTGGATGCTTCTACTCCTTTTAAGGAAGGTCTGTCCTGGCAGGCCCACCGCGCTCCTGTTCTCGCATTGGTTTTCACTGCTTACGGTGATATGTGGTCAGGTTCTGAGGGTGGTGTTATTAAAATCTGGCCATGGGAAACCATAGAAAAATCTCTCTCTCTAAAACCAGAGGAAAGGCATATGGCAGCTTTACTGGTGGAGAGGTCAGGCATTGACCTGAGGAGCCAAGTTACTGTGAATGGCGTCTGTAGTATATCTTCTCAAGATGTCAAGTGTTTGCTATCTGATGATTTTAGAGCTAAAGTTTGGTGTAGCGGATCTATGTCCTTCTCCTTGTGGGATGCTCGTACAAGAGAGCTTGTGAAAGTCTTTAATGTAGAAGGTCAGATGGAGAATCGAGTTGACATGTCAGCAGTGCAGCAAGATCAAGCAGTAGAAGATGAGATGAAGGTAAAATTTGTTTCAACATCGAAAAAGGAGAAATCCGGGGGCTTTTTACAACGGTCGCGTAATGCTATAATGGGAGCTGCAGATGCTGTTCGTCGGGTTGCAACAAGAGGGGCAGGGGCATTTGTAGAAGAATCCAAGAAGACAGAAGCGTTAGTGCTAACTGCGGATGGCATGATATGGAGTGGATGCACAAATGGTTTACTGGTTCAGTGGGATGGAAATGGTAACCGTGTGCAAGATTTTAATCACCATTCTTCTGGTGTCCAATGCTTTTGCACTCTCGGAACACGAATATATGTAGGCTATGTGAGTGGCATGATTCAGGTGTTGGATCTTGAGGGAAATCTGATTGCAGGATGGGTTGCACACAGTAGTCCAGTGATAAAACTGGCGGTTGGAACTGGTTTTGTTTATAGCTTGGCCACCCATGGTGGTATTCGCGGATGGAACATCAAATCTCCAGGACCTACTGACAACTTGATACGTACAGAATTGGCTGCAAAAGAAAGTGTATATACCAAAAGTGACAATGTTAGAATTTTAGTTGGCACATGGAATGTTGGTCAAGGAAGAGCATCTCAGGATGCACTAATATCATGGTTAGGCTCTGCTGTGCCGGATGTTGGCATTGTAGTTGTCGGGTTGCAAGAAGTAGAAATGGGCGCAGGCTTTCTTGCAATGTCTGCAGCAAAAGAAACTGTAGGGCTTGAAGGGAGTTCTGTTGGTAACTGGTGGCTTGATAACATAGGAAAGGCCTTAGAAGAAGGAAAAACTTTTGAACGTATGGGTTCTAGGCAGCTCGCAGGCTTGCTCATATCTCTTTGGGTAAGAAAGAATCTCAGAAAACATGTCGGGGATATTGATGCTGGAGCAGTTCCCTGTGGATTTGGTCGTGCCATTGGTAATAAGGGGGGTGTTGGTCTGAGAATCCGAGTCTATGATCGGATAATGTGCTTTGTGAACTGTCATTTGGCTGCACATTTGGAAGCTGTCAGTCGCCGTAATGCTGATTTTGATCACATTTATCGTAATATGGTCTTCACCCGGTCGTCTCTACTTAACACAGCAGCTGCTGGTGTCTCAACTTCTGTTAACATGGCTCGGGCTCCAAATGCTGTCAGTAGCAACACTGAAGATGTAAGGCCCGAATTAGCAGAAGCAGATATGGTTGTTTTTCTCGGTGATTTCAATTATCGGCTTTTTGGCATATCTTATGATGAAGCAAGGGACTTCGTTTCGCAAAGATGCTTTGATTGGCTCAGAGAAAAAGATCAGCTCAGAGCAGAGATGAAAGCCGGGAAAGTTTTCCAAGGGATGCGTGAGGCACTCATCAGATTCCCTCCAACATACAAGTTTGAAAAGCACCAAGCAGGTTTAGCAGGATATGATTCTGGTGAGAAGAAACGTATTCCTGCATGGTGTGATAGAATAATATATCGTGACAATCGGCCATCACCAGTTGCTGAGTGCAGTTTAGAGTGCCCTGTAGTCTCGTCAATTATACAGTATGAGGCATGCATGGATGTGACTGACAGTGATCACAAACCTGTCCGGTGTAAACTAAGTTTACAAATTGCGCACGTTGATAGATCAGTAAGGAGAAAGGAGTTTGGGGAAATTATAAAGTCCAATCAGAATATCAGGTCTATGCTTGAAGAATCAAATTATGTTCCAGAAACTATTTTGAACACCAACAACATAATCCTTCAAAATCAGGACACGTCCATTTTGCGTATCACTAATACATGTGTAAAGGATACGGCCGTATTTAAAATCATCTGTGAAGGTCAGTCCACCGTCAAGGAGGATGAGGAAGAGCCTGACTATCGCCCGAGAGGTGCCCATGGGTTTCCTCGATGGCTTGAGATCACACCAGCAGCGGGCATGATCAAACCTGAGGAGTGTGTGGAGGTCTCAGTTCATCATGAAGAGTTTCATACCTTGGAAGAATTTGTTGATGGCATCCCTCAAAATTGGTGGTGCGAAGACACCCGCGACAAGGAAGTGATTTTAATGTTGAGTGTCCAAGGTAGTTGCTCAGCTCAAACATATAGTCACAGAGTTCGTGTACGGCATTGCTTCTCAGCCAAGACAGTCCGCATGGACTCAAAATCCAACAGCTCTAGGAGAGGCCAGGGAAGTCCAGTTCACAGGTCTGAAGTTCGGCAACTTAACATACCAAGCCAAATCAACTTGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005975 GO:0006066 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009056 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009611 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009719 GO:0009725 GO:0009737 GO:0009743 GO:0009756 GO:0009846 GO:0009856 GO:0009888 GO:0009987 GO:0009991 GO:0010033 GO:0010087 GO:0010182 GO:0010252 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0019751 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0042578 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043647 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044282 GO:0044424 GO:0044464 GO:0044706 GO:0046030 GO:0046164 GO:0046174 GO:0046434 GO:0046838 GO:0046855 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051716 GO:0052658 GO:0052745 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071545 GO:0071704 GO:0097305 GO:0099402 GO:1901575 GO:1901615 GO:1901616 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04070 map00562 map01100 map04070

Pfam Domains

Protein Families

Exo_endo_phos

Protein Analysis

1146

Amino Acids

126.92

Weight (kDa)

6.48

Isoelectric Point (pI)

42.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_IP5PC_F	PF23754	168 - 561	2e-227	IP5P C-F beta-propeller
Exo_endo_phos2	PF22669	595 - 930	1.3e-64	Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos	PF03372	595 - 926	3.2e-07	Endonuclease/Exonuclease/phosphatase family
Ig-like_IP5PC_F	PF23755	969 - 1105	8.9e-73	IP5P C-F Ig-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004616)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05630 AT1G05630 AT1G05630 AT2G31830 AT2G31830 AT2G31830 AT2G43900 AT2G43900
fragaria_vesca	FvH4_5g18650 FvH4_5g18650 FvH4_5g18650 FvH4_5g18650
malus_domestica	MD00G1087600.v1.1 MD15G1143600.v1.1
prunus_persica	Prupe.7G062000_v2.0.a1
pyrus_communis	pycom15g12950 pycom15g12960 pycom15g12970 pycom290g00230
rosa_chinensis	RchiOBHm_Chr7g0176851
rosa_laevigata	RLG00000005579
rosa_multiflora	Rmu_co8238923.1_g000001 Rmu_sc0005310.1_g000054 Rmu_sc0027976.1_g000001
rosa_roxburghii	Rroxscaffold_3G00276340
rosa_rugosa	Rorug06G0401200
rosa_samantha	Rh7AG000500 Rh7BG000900 Rh7CG000500 Rh7DG000700
rosa_wichuraiana	Rw7G000040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2858
AasI	GACNNNNNNGTC	1 cut(s)	158
AatII	GACGTC	1 cut(s)	156
Acc16I	TGCGCA	1 cut(s)	2814
Acc36I	ACCTGC	2 cut(s)	513, 2597
AccB1I	GGYRCC	2 cut(s)	311, 3074
AccB7I	CCANNNNNTGG	2 cut(s)	331, 394
AccI	GTMKAC	1 cut(s)	195
AccII	CGCG	5 cut(s)	450, 837, 1294, 1677, 3231
AclWI	GGATC	4 cut(s)	359, 786, 1111, 1575
AcoI	YGGCCR	7 cut(s)	396, 568, 742, 914, 1656, 2684, 2997
AcsI	RAATTY	5 cut(s)	401, 595, 1241, 1773, 3185
AcuI	CTGAAG	2 cut(s)	2388, 3414
AcyI	GRCGYC	3 cut(s)	153, 606, 1029
AdeI	CACNNNGTG	1 cut(s)	301
AfaI	GTAC	4 cut(s)	305, 1135, 1723, 3306
AflIII	ACRYGT	5 cut(s)	300, 1191, 2083, 2955, 2981
AhlI	ACTAGT	1 cut(s)	269
AjiI	CACGTC	2 cut(s)	301, 2958
AjnI	CCWGG	7 cut(s)	463, 513, 570, 616, 821, 1696, 3369
AjuI	GAANNNNNNNTTGG	4 cut(s)	606, 638, 2189, 2221
AloI	GAACNNNNNNTCC	2 cut(s)	3366, 3398
Alw26I	GTCTC	6 cut(s)	484, 503, 2292, 2320, 2731, 3154
AlwI	GGATC	4 cut(s)	359, 786, 1111, 1575
AlwNI	CAGNNNCTG	7 cut(s)	331, 591, 890, 1322, 2306, 2309, 2699
Ama87I	CYCGRG	2 cut(s)	2337, 3067
ApoI	RAATTY	5 cut(s)	401, 595, 1241, 1773, 3185
ArsI	GACNNNNNNTTYG	2 cut(s)	3274, 3306
Asp700I	GAANNNNTTC	2 cut(s)	2540, 3168
AspLEI	GCGC	4 cut(s)	452, 839, 1901, 2815
AspS9I	GGNCC	5 cut(s)	334, 534, 829, 1700, 2379
AsuC2I	CCSGG	3 cut(s)	1273, 2281, 2533
AvaI	CYCGRG	2 cut(s)	2337, 3067
AvaII	GGWCC	3 cut(s)	334, 534, 1700
AxyI	CCTNAGG	3 cut(s)	530, 1004, 3132
BaeGI	GKGCMC	3 cut(s)	314, 2720, 3079
BaeI	ACNNNNGTAYC	2 cut(s)	2985, 3018
BalI	TGGCCA	4 cut(s)	570, 744, 916, 1658
BanI	GGYRCC	2 cut(s)	311, 3074
BanII	GRGCYC	1 cut(s)	2344
BarI	GAAGNNNNNNTAC	2 cut(s)	1027, 1059
BbsI	GAAGAC	5 cut(s)	24, 154, 1382, 2266, 3228
BceAI	ACGGC	5 cut(s)	376, 2220, 2984, 3012, 3323
BciT130I	CCWGG	7 cut(s)	465, 515, 572, 618, 823, 1698, 3371
BciVI	GTATCC	1 cut(s)	2986
BclI	TGATCA	4 cut(s)	292, 2251, 2773, 3123
BcnI	CCSGG	3 cut(s)	1273, 2281, 2533
BcoDI	GTCTC	6 cut(s)	484, 503, 2292, 2320, 2731, 3154
BcuI	ACTAGT	1 cut(s)	269
BfaI	CTAG	3 cut(s)	270, 2028, 3360
BfmI	CTRYAG	5 cut(s)	1033, 1314, 1920, 1935, 2721
BfuAI	ACCTGC	2 cut(s)	513, 2597
BfuI	GTATCC	1 cut(s)	2986
BglI	GCCNNNNNGGC	1 cut(s)	576
BlpI	GCTNAGC	2 cut(s)	47, 3274
Bme18I	GGWCC	3 cut(s)	334, 534, 1700
BmeT110I	CYCGRG	2 cut(s)	2337, 3067
BmgBI	CACGTC	2 cut(s)	301, 2958
BmgT120I	GGNCC	5 cut(s)	334, 534, 829, 1700, 2379
BmiI	GGNNCC	6 cut(s)	313, 441, 527, 1010, 2343, 3076
BoxI	GACNNNNGTC	1 cut(s)	515
BpiI	GAAGAC	5 cut(s)	24, 154, 1382, 2266, 3228
BpmI	CTGGAG	2 cut(s)	1680, 2124
Bpu1102I	GCTNAGC	2 cut(s)	47, 3274
BpuEI	CTTGAG	3 cut(s)	1032, 1592, 3119
BpuMI	CCSGG	3 cut(s)	1273, 2281, 2533
BsaAI	YACGTR	2 cut(s)	307, 1721
BsaBI	GATNNNNATC	2 cut(s)	1479, 2250
BsaHI	GRCGYC	3 cut(s)	153, 606, 1029
BsaI	GGTCTC	3 cut(s)	484, 503, 3154
BsaJI	CCNNGG	9 cut(s)	513, 917, 1272, 1663, 2545, 3079, 3177, 3262, 3370
BsaWI	WCCGGW	1 cut(s)	2787
BsaXI	ACNNNNNCTCC	2 cut(s)	573, 603
Bse118I	RCCGGY	1 cut(s)	719
Bse21I	CCTNAGG	3 cut(s)	530, 1004, 3132
Bse3DI	GCAATG	4 cut(s)	537, 700, 1920, 3310
Bse8I	GATNNNNATC	2 cut(s)	1479, 2250
BseBI	CCWGG	7 cut(s)	465, 515, 572, 618, 823, 1698, 3371
BseDI	CCNNGG	9 cut(s)	513, 917, 1272, 1663, 2545, 3079, 3177, 3262, 3370
BseJI	GATNNNNATC	2 cut(s)	1479, 2250
BseMI	GCAATG	4 cut(s)	537, 700, 1920, 3310
BseRI	GAGGAG	2 cut(s)	1021, 3149
BseSI	GKGCMC	3 cut(s)	314, 2720, 3079
BseX3I	CGGCCG	1 cut(s)	2997
BseYI	CCCAGC	1 cut(s)	555
BsgI	GTGCAG	4 cut(s)	529, 1223, 2196, 2725
Bsh1236I	CGCG	5 cut(s)	450, 837, 1294, 1677, 3231
Bsh1285I	CGRYCG	3 cut(s)	1292, 2285, 3000
BshNI	GGYRCC	2 cut(s)	311, 3074
BsiEI	CGRYCG	3 cut(s)	1292, 2285, 3000
BsiHKCI	CYCGRG	2 cut(s)	2337, 3067
BsiSI	CCGG	6 cut(s)	720, 1272, 1853, 2281, 2532, 2788
BslFI	GGGAC	3 cut(s)	44, 456, 2477
BsmAI	GTCTC	6 cut(s)	484, 503, 2292, 2320, 2731, 3154
BsmBI	CGTCTC	1 cut(s)	2292
BsmFI	GGGAC	3 cut(s)	44, 456, 2477
BsmI	GAATGC	1 cut(s)	389
Bso31I	GGTCTC	3 cut(s)	484, 503, 3154
BsoBI	CYCGRG	2 cut(s)	2337, 3067
Bsp1286I	GDGCHC	4 cut(s)	314, 2344, 2720, 3079
Bsp1720I	GCTNAGC	2 cut(s)	47, 3274
Bsp19I	CCATGG	3 cut(s)	917, 1663, 3079
BspFNI	CGCG	5 cut(s)	450, 837, 1294, 1677, 3231
BspHI	TCATGA	2 cut(s)	642, 3160
BspLI	GGNNCC	6 cut(s)	313, 441, 527, 1010, 2343, 3076
BspMAI	CTGCAG	2 cut(s)	1318, 1924
BspMI	ACCTGC	2 cut(s)	513, 2597
BspPI	GGATC	4 cut(s)	359, 786, 1111, 1575
BspQI	GCTCTTC	2 cut(s)	1801, 3045
BspT107I	GGYRCC	2 cut(s)	311, 3074
BspTNI	GGTCTC	3 cut(s)	484, 503, 3154
BsrDI	GCAATG	4 cut(s)	537, 700, 1920, 3310
BsrFI	RCCGGY	1 cut(s)	719
BssAI	RCCGGY	1 cut(s)	719
BssECI	CCNNGG	9 cut(s)	513, 917, 1272, 1663, 2545, 3079, 3177, 3262, 3370
BssNI	GRCGYC	3 cut(s)	153, 606, 1029
BssT1I	CCWWGG	6 cut(s)	917, 1663, 2545, 3079, 3177, 3262
Bst2UI	CCWGG	7 cut(s)	465, 515, 572, 618, 823, 1698, 3371
Bst6I	CTCTTC	4 cut(s)	214, 1801, 3045, 3159
BstACI	GRCGYC	3 cut(s)	153, 606, 1029
BstAPI	GCANNNNNTGC	1 cut(s)	1919
BstBAI	YACGTR	2 cut(s)	307, 1721
BstDSI	CCRYGG	3 cut(s)	917, 1663, 3079
BstEII	GGTNACC	1 cut(s)	1460
BstFNI	CGCG	5 cut(s)	450, 837, 1294, 1677, 3231
BstHHI	GCGC	4 cut(s)	452, 839, 1901, 2815
BstMAI	GTCTC	6 cut(s)	484, 503, 2292, 2320, 2731, 3154
BstMCI	CGRYCG	3 cut(s)	1292, 2285, 3000
BstMWI	GCNNNNNNNGC	8 cut(s)	107, 420, 576, 836, 1319, 1919, 2348, 2612
BstNI	CCWGG	7 cut(s)	465, 515, 572, 618, 823, 1698, 3371
BstNSI	RCATGY	4 cut(s)	1195, 2087, 2754, 2985
BstPAI	GACNNNNGTC	1 cut(s)	515
BstPI	GGTNACC	1 cut(s)	1460
BstSFI	CTRYAG	5 cut(s)	1033, 1314, 1920, 1935, 2721
BstSLI	GKGCMC	3 cut(s)	314, 2720, 3079
BstSNI	TACGTA	1 cut(s)	1721
BstUI	CGCG	5 cut(s)	450, 837, 1294, 1677, 3231
BstV2I	GAAGAC	5 cut(s)	24, 154, 1382, 2266, 3228
BstX2I	RGATCY	2 cut(s)	1103, 1567
BstXI	CCANNNNNNTGG	2 cut(s)	752, 1493
BstYI	RGATCY	2 cut(s)	1103, 1567
BstZI	CGGCCG	1 cut(s)	2997
Bsu36I	CCTNAGG	3 cut(s)	530, 1004, 3132
BsuI	GTATCC	1 cut(s)	2986
BtgI	CCRYGG	3 cut(s)	917, 1663, 3079
BtrI	CACGTC	2 cut(s)	301, 2958
BtsI	GCAGTG	2 cut(s)	861, 1206
BtsIMutI	CAGTG	7 cut(s)	423, 861, 1206, 1451, 1620, 2364, 2776
BveI	ACCTGC	2 cut(s)	513, 2597
CaiI	CAGNNNCTG	7 cut(s)	331, 591, 890, 1322, 2306, 2309, 2699
CciI	TCATGA	2 cut(s)	642, 3160
CfoI	GCGC	4 cut(s)	452, 839, 1901, 2815
Cfr10I	RCCGGY	1 cut(s)	719
Cfr13I	GGNCC	5 cut(s)	334, 534, 829, 1700, 2379
CseI	GACGC	4 cut(s)	41, 148, 595, 1018
CsiI	ACCWGGT	1 cut(s)	616
Csp6I	GTAC	4 cut(s)	304, 1134, 1722, 3305
CspCI	CAANNNNNGTGG	2 cut(s)	3194, 3229
CviQI	GTAC	4 cut(s)	304, 1134, 1722, 3305
DraI	TTTAAA	1 cut(s)	3007
DraIII	CACNNNGTG	1 cut(s)	301
DrdI	GACNNNNNNGTC	1 cut(s)	158
DseDI	GACNNNNNNGTC	1 cut(s)	158
EaeI	YGGCCR	7 cut(s)	396, 568, 742, 914, 1656, 2684, 2997
EagI	CGGCCG	1 cut(s)	2997
Eam1104I	CTCTTC	4 cut(s)	214, 1801, 3045, 3159
EarI	CTCTTC	4 cut(s)	214, 1801, 3045, 3159
EciI	GGCGGA	3 cut(s)	97, 594, 670
EclXI	CGGCCG	1 cut(s)	2997
Eco105I	TACGTA	1 cut(s)	1721
Eco130I	CCWWGG	6 cut(s)	917, 1663, 2545, 3079, 3177, 3262
Eco147I	AGGCCT	1 cut(s)	1991
Eco24I	GRGCYC	1 cut(s)	2344
Eco31I	GGTCTC	3 cut(s)	484, 503, 3154
Eco47I	GGWCC	3 cut(s)	334, 534, 1700
Eco52I	CGGCCG	1 cut(s)	2997
Eco57I	CTGAAG	2 cut(s)	2388, 3414
Eco81I	CCTNAGG	3 cut(s)	530, 1004, 3132
Eco88I	CYCGRG	2 cut(s)	2337, 3067
Eco91I	GGTNACC	1 cut(s)	1460
EcoO109I	RGGNCCY	1 cut(s)	1700
EcoO65I	GGTNACC	1 cut(s)	1460
EcoRII	CCWGG	7 cut(s)	463, 513, 570, 616, 821, 1696, 3369
EcoT14I	CCWWGG	6 cut(s)	917, 1663, 2545, 3079, 3177, 3262
EcoT22I	ATGCAT	1 cut(s)	2756
EcoT38I	GRGCYC	1 cut(s)	2344
ErhI	CCWWGG	6 cut(s)	917, 1663, 2545, 3079, 3177, 3262
Esp3I	CGTCTC	1 cut(s)	2292
FalI	AAGNNNNNCTT	2 cut(s)	593, 625
FaqI	GGGAC	3 cut(s)	44, 456, 2477
FauI	CCCGC	4 cut(s)	34, 74, 3109, 3236
FauNDI	CATATG	1 cut(s)	966
FbaI	TGATCA	4 cut(s)	292, 2251, 2773, 3123
FblI	GTMKAC	1 cut(s)	195
FriOI	GRGCYC	1 cut(s)	2344
FspBI	CTAG	3 cut(s)	270, 2028, 3360
FspI	TGCGCA	1 cut(s)	2814
GlaI	GCGC	4 cut(s)	451, 838, 1900, 2814
GsaI	CCCAGC	1 cut(s)	559
GsuI	CTGGAG	2 cut(s)	1680, 2124
HapII	CCGG	6 cut(s)	720, 1272, 1853, 2281, 2532, 2788
HgaI	GACGC	4 cut(s)	41, 148, 595, 1018
HhaI	GCGC	4 cut(s)	452, 839, 1901, 2815
Hin1I	GRCGYC	3 cut(s)	153, 606, 1029
Hin6I	GCGC	4 cut(s)	450, 837, 1899, 2813
HinP1I	GCGC	4 cut(s)	450, 837, 1899, 2813
HincII	GTYRAC	3 cut(s)	196, 1189, 2329
HindII	GTYRAC	3 cut(s)	196, 1189, 2329
HpaI	GTTAAC	1 cut(s)	2329
HpaII	CCGG	6 cut(s)	720, 1272, 1853, 2281, 2532, 2788
Hpy99I	CGWCG	6 cut(s)	26, 152, 155, 221, 302, 1332
HpyAV	CCTTC	9 cut(s)	595, 806, 1123, 1160, 1228, 1940, 1994, 2951, 3014
HpyCH4IV	ACGT	8 cut(s)	153, 300, 306, 1720, 2017, 2641, 2817, 2957
HpyF10VI	GCNNNNNNNGC	8 cut(s)	107, 420, 576, 836, 1319, 1919, 2348, 2612
HpySE526I	ACGT	8 cut(s)	153, 300, 306, 1720, 2017, 2641, 2817, 2957
Hsp92I	GRCGYC	3 cut(s)	153, 606, 1029
HspAI	GCGC	4 cut(s)	450, 837, 1899, 2813
Ksp22I	TGATCA	4 cut(s)	292, 2251, 2773, 3123
KspAI	GTTAAC	1 cut(s)	2329
LguI	GCTCTTC	2 cut(s)	1801, 3045
LmnI	GCTCC	7 cut(s)	99, 484, 844, 1008, 1310, 2105, 2347
MabI	ACCWGGT	1 cut(s)	616
MaeI	CTAG	3 cut(s)	270, 2028, 3360
MaeII	ACGT	8 cut(s)	153, 300, 306, 1720, 2017, 2641, 2817, 2957
MaeIII	GTNAC	8 cut(s)	72, 1015, 1460, 1760, 1961, 2675, 2761, 3290
MflI	RGATCY	2 cut(s)	1103, 1567
MhlI	GDGCHC	4 cut(s)	314, 2344, 2720, 3079
MlsI	TGGCCA	4 cut(s)	570, 744, 916, 1658
MluNI	TGGCCA	4 cut(s)	570, 744, 916, 1658
MlyI	GAGTC	4 cut(s)	200, 591, 2178, 3335
MmeI	TCCRAC	5 cut(s)	467, 1545, 1612, 2603, 3375
Mox20I	TGGCCA	4 cut(s)	570, 744, 916, 1658
Mph1103I	ATGCAT	1 cut(s)	2756
MroXI	GAANNNNTTC	2 cut(s)	2540, 3168
MscI	TGGCCA	4 cut(s)	570, 744, 916, 1658
MslI	CAYNNNNRTG	2 cut(s)	750, 2759
Msp20I	TGGCCA	4 cut(s)	570, 744, 916, 1658
MspA1I	CMGCKG	4 cut(s)	414, 559, 2306, 3116
MspI	CCGG	6 cut(s)	720, 1272, 1853, 2281, 2532, 2788
Mva1269I	GAATGC	1 cut(s)	389
MvaI	CCWGG	7 cut(s)	465, 515, 572, 618, 823, 1698, 3371
MvnI	CGCG	5 cut(s)	450, 837, 1294, 1677, 3231
MwoI	GCNNNNNNNGC	8 cut(s)	107, 420, 576, 836, 1319, 1919, 2348, 2612
NciI	CCSGG	3 cut(s)	1273, 2281, 2533
NcoI	CCATGG	3 cut(s)	917, 1663, 3079
NdeI	CATATG	1 cut(s)	966
NlaIV	GGNNCC	6 cut(s)	313, 441, 527, 1010, 2343, 3076
NmuCI	GTSAC	5 cut(s)	72, 1760, 2675, 2761, 3290
NsbI	TGCGCA	1 cut(s)	2814
NsiI	ATGCAT	1 cut(s)	2756
NspI	RCATGY	4 cut(s)	1195, 2087, 2754, 2985
PaeI	GCATGC	1 cut(s)	2754
PagI	TCATGA	2 cut(s)	642, 3160
PceI	AGGCCT	1 cut(s)	1991
PciI	ACATGT	3 cut(s)	1191, 2083, 2981
PciSI	GCTCTTC	2 cut(s)	1801, 3045
PcsI	WCGNNNNNNNCGW	3 cut(s)	156, 201, 303
PctI	GAATGC	1 cut(s)	389
PdmI	GAANNNNTTC	2 cut(s)	2540, 3168
PfeI	GAWTC	9 cut(s)	677, 1180, 1367, 1555, 2071, 2163, 2571, 2624, 2894
PflFI	GACNNNGTC	1 cut(s)	152
PflMI	CCANNNNNTGG	2 cut(s)	331, 394
PfoI	TCCNGGA	1 cut(s)	1696
PleI	GAGTC	4 cut(s)	200, 590, 2177, 3335
PpsI	GAGTC	4 cut(s)	200, 590, 2177, 3335
Ppu21I	YACGTR	2 cut(s)	307, 1721
PpuMI	RGGWCCY	1 cut(s)	1700
PscI	ACATGT	3 cut(s)	1191, 2083, 2981
PshAI	GACNNNNGTC	1 cut(s)	515
PsiI	TTATAA	1 cut(s)	2858
Psp5II	RGGWCCY	1 cut(s)	1700
Psp6I	CCWGG	7 cut(s)	463, 513, 570, 616, 821, 1696, 3369
PspEI	GGTNACC	1 cut(s)	1460
PspFI	CCCAGC	1 cut(s)	555
PspGI	CCWGG	7 cut(s)	463, 513, 570, 616, 821, 1696, 3369
PspN4I	GGNNCC	6 cut(s)	313, 441, 527, 1010, 2343, 3076
PspPI	GGNCC	5 cut(s)	334, 534, 829, 1700, 2379
PspPPI	RGGWCCY	1 cut(s)	1700
PstI	CTGCAG	2 cut(s)	1318, 1924
PstNI	CAGNNNCTG	7 cut(s)	331, 591, 890, 1322, 2306, 2309, 2699
PsuI	RGATCY	2 cut(s)	1103, 1567
PsyI	GACNNNGTC	1 cut(s)	152
PvuII	CAGCTG	2 cut(s)	559, 2306
RsaI	GTAC	4 cut(s)	305, 1135, 1723, 3306
RsaNI	GTAC	4 cut(s)	304, 1134, 1722, 3305
RseI	CAYNNNNRTG	2 cut(s)	750, 2759
SalI	GTCGAC	1 cut(s)	194
SapI	GCTCTTC	2 cut(s)	1801, 3045
Sau96I	GGNCC	5 cut(s)	334, 534, 829, 1700, 2379
SchI	GAGTC	4 cut(s)	200, 591, 2178, 3335
SduI	GDGCHC	4 cut(s)	314, 2344, 2720, 3079
SexAI	ACCWGGT	1 cut(s)	616
SfcI	CTRYAG	5 cut(s)	1033, 1314, 1920, 1935, 2721
SinI	GGWCC	3 cut(s)	334, 534, 1700
SmiMI	CAYNNNNRTG	2 cut(s)	750, 2759
SmlI	CTYRAG	3 cut(s)	1047, 1571, 3098
SmoI	CTYRAG	3 cut(s)	1047, 1571, 3098
SnaBI	TACGTA	1 cut(s)	1721
SpeI	ACTAGT	1 cut(s)	269
SphI	GCATGC	1 cut(s)	2754
SseBI	AGGCCT	1 cut(s)	1991
SspMI	CTAG	3 cut(s)	270, 2028, 3360
StuI	AGGCCT	1 cut(s)	1991
StyI	CCWWGG	6 cut(s)	917, 1663, 2545, 3079, 3177, 3262
TaiI	ACGT	8 cut(s)	156, 303, 309, 1723, 2020, 2644, 2820, 2960
TaqI	TCGA	5 cut(s)	147, 195, 1183, 1256, 3091
TauI	GCSGC	2 cut(s)	417, 665
TfiI	GAWTC	9 cut(s)	677, 1180, 1367, 1555, 2071, 2163, 2571, 2624, 2894
TscAI	CASTG	7 cut(s)	430, 868, 1206, 1451, 1620, 2371, 2776
TseFI	GTSAC	5 cut(s)	72, 1760, 2675, 2761, 3290
Tsp45I	GTSAC	5 cut(s)	72, 1760, 2675, 2761, 3290
TspDTI	ATGAA	9 cut(s)	69, 167, 690, 1247, 2469, 2540, 3146, 3161, 3177
TspGWI	ACGGA	2 cut(s)	86, 445
TspRI	CASTG	7 cut(s)	430, 868, 1206, 1451, 1620, 2371, 2776
Tth111I	GACNNNGTC	1 cut(s)	152
Van91I	CCANNNNNTGG	2 cut(s)	331, 394
VpaK11BI	GGWCC	3 cut(s)	334, 534, 1700
XapI	RAATTY	5 cut(s)	401, 595, 1241, 1773, 3185
XceI	RCATGY	4 cut(s)	1195, 2087, 2754, 2985
XcmI	CCANNNNNNNNNTGG	1 cut(s)	563
XmiI	GTMKAC	1 cut(s)	195
XmnI	GAANNNNTTC	2 cut(s)	2540, 3168
XspI	CTAG	3 cut(s)	270, 2028, 3360
ZraI	GACGTC	1 cut(s)	154
Zsp2I	ATGCAT	1 cut(s)	2756

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.