Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004616

Physical Location & Seq

Genomic Coordinates Forward (+)

10596103 .. 10600968

Gene Structure 4866 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1143600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3444 bp

ATGGAGGAGAGAGGTGAGGACGACGACAGGGACGCATTGGCGGGCCTAAGCAACGCCCCTCCGCCCCGCAAATCCCATTCATTGAGCCATCAGCTGCGCCCAACCTCCGCCCAGAAACGCCACCACCAAATACGCAAACATAGCCTCGACGACGTCCACGTTGCCCCCAAGAGCGTTAATAGCAATAATAATGTGGACTACTACGACTCTTCTGACGACGATTTCTTTCCTTACTCCACCTCCAACAGCAGCACCACCACCACCACTAGTAATGCTGTTGGCGGTGCTGATTACGAAGTGTACGTGGGCGGCACTCACTCCCAGAGGCTGGACCAAAGCCTCTGCATGGAGGATGGTGATGATCCTGATGGCAACAGAGAATGCCAGCCGTTGCCCGAATTCATTGGCAGCGGCGGCGGCACTGGCATCTTCAAGGTTCCAACTCGCGCCTCCGTCCATCCTGGTCGTCCCCCCTGCTTGGAGCTGAGACCCCACCCTCTCAGGGAGACACAGGTCGGCAGGTTCCTCAGGACCATTGCCTGCACCAACACCCAGCTGTGGGCTGGCCAGGAGGGCGGAGTCAGGGTCTGGAATTTGAAGGACGTCTTTGAACCTGGCTTTGGTATTGCTGGCAGAGTCATGAGGGGTGATGAGGACGCCGCCCCATACATTGAATCCGTCAACACCTCTCCCACCCATTGTCTCACGGTTGATACTGGCAATCGCTTGATTTGGACTGGCCATAAGGATGGCAAGATCAGGTCTTGGAAGATGGATCAGTCTTTGGATGCTTCTACACCTTTTAAGGAAGGTCTTTCCTGGCAGGCCCACCGTGCTCCTGTTCTCGCCATGGTTTTCACTGCTTACGGTGATATGTGGTCAGGTTCCGAGGGCGGTGTTATCAAAATCTGGCCATGGGAAACCATTGAAAAATCTCTCTCCCTAAAACCTGAGGAAAGGCATATGGCAGCTTTACTGGTGGAGAGGTCGTGCATTGACCTCAGGAGCCAAGTTACTGTTAATGGCGTCTGTAGTATATCTTCTCAAGATGTCAAGTGTTTGGTTTCTGATAATTTCAGAGCTAAAGTTTGGTGTGCCGGTTCTATGTCCTTCTCCTGGTGGGATGCTCGTACAAGAGAGCTTGTGAAAGTCTTTAATGTAGAAGGTCAGATTGAGAATCGAGTTGACATGTCAGCAGTGCAGCAAGATCAAGCAGTAGAAGATGAGATGAAGGTAAAATTTGTTTCAACATCCAAAAGGGAGAAATCCGGGGGCTTTCTACAACGGTCGCGTAATGCTATAATGGGAGCTGCAGATGCTGTTCGACGAGTTGCAACAAGAGGGGCTGGGGCATTTGTAGAAGAATCCAAGAAGACAGAAGCGCTGGTGCTAACTGCGGATGGCATGATATGGAGCGGATGCACAAATGGTTTACTGGTTCAGTGGGATGGAAATGGTAACCGTGTGCAAGATTTTAATCACCATTATTCTGGTGTCCAATGCTTTTGCACTCTTGGGACACGAATATATGTAGGCTATGTGAGTGGCATGATTCAGGTATTGGATCTTGAGGGAAATCTGATTGCAGGATGGGTTGCACACAGTAGTCCAGTGATAAAACTGGCCGTTGGAACTGGTGTTGTTTATAGCTTGGCCACCCATGGTGGTATTCGCGGATGGCACATCAAATCTCCAGGACCTACAGACAACTTGATACGTACAGAATTGGCTGCAAAAGAAAGTGTATATACCAAAAGAGACAATGTTAGAATTTTAGTAGGCTCATGGAATGTTGGTCAAGGAAGAGCATCTCAGGATGCACTAATGTCATGGTTGGGCTCTGCTGTGCCGGATGTTGGCATTGTAGTTGTCGGGTTACAAGAAGTAGAAATGGGCGCAGGCTTTCTTGCAATGTCTGCAGCAAAAGAAACTGTAGGGCTTGAAGGGAGTTCTGTTGGCAACTGGTGGCTTGATAATATAGGAAAGGCCTTAGAAGAAGGAAAAACTTTCGAACGTATGGGTTCTAGGCAGCTTGCAGGCTTGCTCATATCTCTTTGGGTAAGAAAGAATCTCAGAACACATGTTGGGGATATTGATGCTGGAGCAGTTCCGTGTGGATTTGGTCGTGCCATTGGTAACAAGGGAGGTGTTGGTTTGAGAATCCGAGTCTATGATCGTATAATGTGCTTTGTGAACTGTCATTTGGCTGCACATTTGGAAGCTGTCAATCGCCGTAATGCTGATTTTGATCACATTTATCGAAATATGGTCTTCAACCGGTCATCTCTACTGAACACGGCAGCTGCTGGTGTCTCAACTTCTGTTAATATGTCTCGAGCTCCAAATGCTGTCAGCAGCAACACTGAAGATATAAGGCCTGAATTAGCTGAAGCAGATGTGGTTGTTTTTCTCGGTGATTTCAATTATCGGCTTTTTGGTATATCTTACGATGAAGCAAGGGACTTTGTTTCACAAAGATGCTTTGATTGGCTCAGAGAAAAAGATCAGCTCAGAGCAGAGATGAAAGCCGGGAAAGTTTTCCAAGGGATGCGTGAGGCACTCATCAGATTCCCTCCAACATACAAGTTTGAAAGGCACCAAGCAGGTTTAGCAGGATATGATTCTGGTGAGAAGAAACGCATTCCTGCCTGGTGCGATAGAATAATATATCGTGACAATCGGCCATTACCAGTTGCTGAGTGCAGTTTAGAGTGCCCTGTAGTCTCGTCAATTATACAGTACGAGGCACGCATGGACGTGACTGACAGTGATCACAAACCTGTCCGGTGTAAACTAAGTTTACAAATTGCTCATGTTGATAGATCGGTAAGGAGAAAGGAGTTTGGGGAAATTGTAAAATCCAATCAGAATATCAGGTCTAATCTTGAAGATTCAAATTATGTTCCAGAAACTATTTTGAACACCAACAACATAATCCTTCAAAATCAGGACACGTCCATTTTGTGTATCACTAATAAATGTGTAAAGGATACTGCCGTATTTAAAATCACTTGTGAAGGCCAGTCCACTGTCAAGGAGGATGGCGAAGAGCCTAACTATCGCCCGAGAGGTGCCAACGGGTTTCCTCGATGGCTTGAGATCACACCAGCAGCAGGCATGATCAAACCTGAACAGTCTGTGGAGGTCTCAGTTCATCACGAAGAGTTTCATACCTTGGAAGAATTTGTTGATGGCATCCCTCAAAATTGGTGGTGCGAAGACACCCGCGACAAGGAAGCCATTTTAATGGTCAGTGTCCAAGGTAGTTGCTCAGCTCAAACATGTAGTCACAGAGTTCGAGTGCGGCATTGCTTCTCAGCCAAGACCCGCATGGACTCAAAATCCAACAGCTCTAAAAAATGCCAGGGAAGTCCAGTTCACAGGTCTGAAGTTCGGCAACCTAACATTTCAAGCGAAACCAACTTAAAAAATCCTAGAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005975 GO:0006066 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009056 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009611 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009719 GO:0009725 GO:0009737 GO:0009743 GO:0009756 GO:0009846 GO:0009856 GO:0009888 GO:0009987 GO:0009991 GO:0010033 GO:0010087 GO:0010182 GO:0010252 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0019751 GO:0022414 GO:0022622 GO:0023052 GO:0031667 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0042578 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043647 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044282 GO:0044424 GO:0044464 GO:0044706 GO:0046030 GO:0046164 GO:0046174 GO:0046434 GO:0046838 GO:0046855 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051716 GO:0052658 GO:0052745 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071545 GO:0071704 GO:0097305 GO:0099402 GO:1901575 GO:1901615 GO:1901616 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04070 map00562 map01100 map04070

Pfam Domains

Protein Families

Exo_endo_phos

Protein Analysis

1148

Amino Acids

127.16

Weight (kDa)

6.7

Isoelectric Point (pI)

43.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_IP5PC_F	PF23754	167 - 560	2.1e-226	IP5P C-F beta-propeller
Exo_endo_phos	PF03372	594 - 925	4.1e-07	Endonuclease/Exonuclease/phosphatase family
Exo_endo_phos2	PF22669	595 - 929	4.4e-65	Endonuclease/Exonuclease/phosphatase family 2
Ig-like_IP5PC_F	PF23755	968 - 1104	9.7e-72	IP5P C-F Ig-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004616)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05630 AT1G05630 AT1G05630 AT2G31830 AT2G31830 AT2G31830 AT2G43900 AT2G43900
fragaria_vesca	FvH4_5g18650 FvH4_5g18650 FvH4_5g18650 FvH4_5g18650
malus_domestica	MD00G1087600.v1.1 MD15G1143600.v1.1
prunus_persica	Prupe.7G062000_v2.0.a1
pyrus_communis	pycom15g12950 pycom15g12960 pycom15g12970 pycom290g00230
rosa_chinensis	RchiOBHm_Chr7g0176851
rosa_laevigata	RLG00000005579
rosa_multiflora	Rmu_co8238923.1_g000001 Rmu_sc0005310.1_g000054 Rmu_sc0027976.1_g000001
rosa_roxburghii	Rroxscaffold_3G00276340
rosa_rugosa	Rorug06G0401200
rosa_samantha	Rh7AG000500 Rh7BG000900 Rh7CG000500 Rh7DG000700
rosa_wichuraiana	Rw7G000040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	156, 606
Acc36I	ACCTGC	2 cut(s)	510, 2594
AccB1I	GGYRCC	2 cut(s)	2595, 3071
AccB7I	CCANNNNNTGG	1 cut(s)	328
AccBSI	CCGCTC	1 cut(s)	1416
AccII	CGCG	4 cut(s)	447, 1291, 1674, 3228
AclWI	GGATC	3 cut(s)	356, 783, 1572
AcoI	YGGCCR	6 cut(s)	565, 739, 911, 1623, 1653, 2681
AcsI	RAATTY	5 cut(s)	398, 592, 1238, 1770, 3182
AcuI	CTGAAG	3 cut(s)	2385, 2409, 3408
AcyI	GRCGYC	4 cut(s)	153, 603, 657, 1026
AfaI	GTAC	4 cut(s)	302, 1132, 1720, 2741
AfeI	AGCGCT	1 cut(s)	1383
AflIII	ACRYGT	4 cut(s)	1188, 2080, 2952, 3281
AgeI	ACCGGT	1 cut(s)	2277
AhlI	ACTAGT	1 cut(s)	266
AjiI	CACGTC	2 cut(s)	2758, 2955
AjnI	CCWGG	8 cut(s)	460, 567, 613, 818, 1115, 1693, 2648, 3363
AjuI	GAANNNNNNNTTGG	4 cut(s)	603, 635, 2186, 2218
AloI	GAACNNNNNNTCC	2 cut(s)	3360, 3392
Alw21I	GWGCWC	2 cut(s)	838, 2341
Alw26I	GTCTC	8 cut(s)	481, 500, 707, 1752, 2317, 2337, 2728, 3151
AlwI	GGATC	3 cut(s)	356, 783, 1572
AlwNI	CAGNNNCTG	5 cut(s)	328, 588, 1319, 2306, 2696
Ama87I	CYCGRG	2 cut(s)	2334, 3064
Aor51HI	AGCGCT	1 cut(s)	1383
ApoI	RAATTY	5 cut(s)	398, 592, 1238, 1770, 3182
ArsI	GACNNNNNNTTYG	2 cut(s)	3271, 3303
AsiGI	ACCGGT	1 cut(s)	2277
Asp700I	GAANNNNTTC	2 cut(s)	2537, 3165
AspLEI	GCGC	4 cut(s)	99, 449, 1384, 1898
AspS9I	GGNCC	5 cut(s)	43, 331, 531, 826, 1697
AsuC2I	CCSGG	2 cut(s)	1270, 2530
AsuHPI	GGTGA	7 cut(s)	26, 368, 659, 881, 1472, 2426, 2639
AsuII	TTCGAA	1 cut(s)	2010
AvaI	CYCGRG	2 cut(s)	2334, 3064
AvaII	GGWCC	3 cut(s)	331, 531, 1697
AxyI	CCTNAGG	3 cut(s)	527, 951, 1001
BaeGI	GKGCMC	1 cut(s)	2717
BaeI	ACNNNNGTAYC	2 cut(s)	2982, 3015
BalI	TGGCCA	4 cut(s)	567, 741, 913, 1655
BanI	GGYRCC	2 cut(s)	2595, 3071
BanII	GRGCYC	2 cut(s)	1841, 2341
BarI	GAAGNNNNNNTAC	2 cut(s)	1024, 1056
BbsI	GAAGAC	3 cut(s)	1379, 2263, 3225
Bbv12I	GWGCWC	2 cut(s)	838, 2341
BceAI	ACGGC	5 cut(s)	373, 1610, 2217, 2313, 2981
BciT130I	CCWGG	8 cut(s)	462, 569, 615, 820, 1117, 1695, 2650, 3365
BciVI	GTATCC	1 cut(s)	2983
BclI	TGATCA	3 cut(s)	2248, 2770, 3120
BcnI	CCSGG	2 cut(s)	1270, 2530
BcoDI	GTCTC	8 cut(s)	481, 500, 707, 1752, 2317, 2337, 2728, 3151
BcuI	ACTAGT	1 cut(s)	266
BfaI	CTAG	3 cut(s)	267, 2025, 3435
BfmI	CTRYAG	6 cut(s)	1030, 1311, 1701, 1917, 1932, 2718
BfoI	RGCGCY	1 cut(s)	1385
BfuAI	ACCTGC	2 cut(s)	510, 2594
BfuI	GTATCC	1 cut(s)	2983
BglI	GCCNNNNNGGC	1 cut(s)	573
BlpI	GCTNAGC	1 cut(s)	3271
Bme18I	GGWCC	3 cut(s)	331, 531, 1697
BmeT110I	CYCGRG	2 cut(s)	2334, 3064
BmgBI	CACGTC	2 cut(s)	2758, 2955
BmgT120I	GGNCC	5 cut(s)	43, 331, 531, 826, 1697
BmiI	GGNNCC	6 cut(s)	438, 524, 886, 1007, 2597, 3073
BoxI	GACNNNNGTC	1 cut(s)	512
BpiI	GAAGAC	3 cut(s)	1379, 2263, 3225
BpmI	CTGGAG	2 cut(s)	1677, 2121
Bpu10I	CCTNAGC	1 cut(s)	47
Bpu1102I	GCTNAGC	1 cut(s)	3271
Bpu14I	TTCGAA	1 cut(s)	2010
BpuEI	CTTGAG	3 cut(s)	1029, 1589, 3116
BpuMI	CCSGG	2 cut(s)	1270, 2530
BsaAI	YACGTR	2 cut(s)	304, 1718
BsaBI	GATNNNNATC	2 cut(s)	1476, 2247
BsaHI	GRCGYC	4 cut(s)	153, 603, 657, 1026
BsaI	GGTCTC	2 cut(s)	481, 3151
BsaJI	CCNNGG	9 cut(s)	849, 888, 914, 1269, 1660, 2542, 3174, 3259, 3364
BsaWI	WCCGGW	2 cut(s)	2277, 2784
BsaXI	ACNNNNNCTCC	6 cut(s)	224, 254, 570, 600, 974, 1004
Bse118I	RCCGGY	2 cut(s)	1097, 2277
Bse21I	CCTNAGG	3 cut(s)	527, 951, 1001
Bse3DI	GCAATG	3 cut(s)	534, 1917, 3307
Bse8I	GATNNNNATC	2 cut(s)	1476, 2247
BseBI	CCWGG	8 cut(s)	462, 569, 615, 820, 1117, 1695, 2650, 3365
BseDI	CCNNGG	9 cut(s)	849, 888, 914, 1269, 1660, 2542, 3174, 3259, 3364
BseJI	GATNNNNATC	2 cut(s)	1476, 2247
BseMI	GCAATG	3 cut(s)	534, 1917, 3307
BseRI	GAGGAG	1 cut(s)	20
BseSI	GKGCMC	1 cut(s)	2717
BseYI	CCCAGC	2 cut(s)	552, 1346
BsgI	GTGCAG	4 cut(s)	526, 1220, 2193, 2722
Bsh1236I	CGCG	4 cut(s)	447, 1291, 1674, 3228
Bsh1285I	CGRYCG	1 cut(s)	1289
BshNI	GGYRCC	2 cut(s)	2595, 3071
BshTI	ACCGGT	1 cut(s)	2277
BsiEI	CGRYCG	1 cut(s)	1289
BsiHKAI	GWGCWC	2 cut(s)	838, 2341
BsiHKCI	CYCGRG	2 cut(s)	2334, 3064
BsiSI	CCGG	6 cut(s)	1098, 1269, 1850, 2278, 2529, 2785
BslFI	GGGAC	4 cut(s)	44, 453, 1531, 2474
BsmAI	GTCTC	8 cut(s)	481, 500, 707, 1752, 2317, 2337, 2728, 3151
BsmFI	GGGAC	4 cut(s)	44, 453, 1531, 2474
BsmI	GAATGC	2 cut(s)	386, 2640
Bso31I	GGTCTC	2 cut(s)	481, 3151
BsoBI	CYCGRG	2 cut(s)	2334, 3064
Bsp119I	TTCGAA	1 cut(s)	2010
Bsp1286I	GDGCHC	4 cut(s)	838, 1841, 2341, 2717
Bsp1720I	GCTNAGC	1 cut(s)	3271
Bsp19I	CCATGG	3 cut(s)	849, 914, 1660
BspFNI	CGCG	4 cut(s)	447, 1291, 1674, 3228
BspHI	TCATGA	1 cut(s)	639
BspLI	GGNNCC	6 cut(s)	438, 524, 886, 1007, 2597, 3073
BspMAI	CTGCAG	2 cut(s)	1315, 1921
BspMI	ACCTGC	2 cut(s)	510, 2594
BspPI	GGATC	3 cut(s)	356, 783, 1572
BspQI	GCTCTTC	2 cut(s)	1798, 3042
BspT104I	TTCGAA	1 cut(s)	2010
BspT107I	GGYRCC	2 cut(s)	2595, 3071
BspTNI	GGTCTC	2 cut(s)	481, 3151
BsrBI	CCGCTC	1 cut(s)	1416
BsrDI	GCAATG	3 cut(s)	534, 1917, 3307
BsrFI	RCCGGY	2 cut(s)	1097, 2277
BssAI	RCCGGY	2 cut(s)	1097, 2277
BssECI	CCNNGG	9 cut(s)	849, 888, 914, 1269, 1660, 2542, 3174, 3259, 3364
BssNI	GRCGYC	4 cut(s)	153, 603, 657, 1026
BssT1I	CCWWGG	6 cut(s)	849, 914, 1660, 2542, 3174, 3259
Bst2UI	CCWGG	8 cut(s)	462, 569, 615, 820, 1117, 1695, 2650, 3365
Bst6I	CTCTTC	4 cut(s)	214, 1798, 3042, 3156
BstACI	GRCGYC	4 cut(s)	153, 603, 657, 1026
BstAPI	GCANNNNNTGC	1 cut(s)	1916
BstBAI	YACGTR	2 cut(s)	304, 1718
BstBI	TTCGAA	1 cut(s)	2010
BstDSI	CCRYGG	3 cut(s)	849, 914, 1660
BstEII	GGTNACC	1 cut(s)	1457
BstFNI	CGCG	4 cut(s)	447, 1291, 1674, 3228
BstH2I	RGCGCY	1 cut(s)	1385
BstHHI	GCGC	4 cut(s)	99, 449, 1384, 1898
BstMAI	GTCTC	8 cut(s)	481, 500, 707, 1752, 2317, 2337, 2728, 3151
BstMCI	CGRYCG	1 cut(s)	1289
BstNI	CCWGG	8 cut(s)	462, 569, 615, 820, 1117, 1695, 2650, 3365
BstNSI	RCATGY	3 cut(s)	1192, 2084, 3285
BstPAI	GACNNNNGTC	1 cut(s)	512
BstPI	GGTNACC	1 cut(s)	1457
BstSFI	CTRYAG	6 cut(s)	1030, 1311, 1701, 1917, 1932, 2718
BstSLI	GKGCMC	1 cut(s)	2717
BstSNI	TACGTA	1 cut(s)	1718
BstUI	CGCG	4 cut(s)	447, 1291, 1674, 3228
BstV2I	GAAGAC	3 cut(s)	1379, 2263, 3225
BstX2I	RGATCY	1 cut(s)	1564
BstXI	CCANNNNNNTGG	3 cut(s)	749, 1490, 3247
BstYI	RGATCY	1 cut(s)	1564
Bsu36I	CCTNAGG	3 cut(s)	527, 951, 1001
BsuI	GTATCC	1 cut(s)	2983
BtgI	CCRYGG	3 cut(s)	849, 914, 1660
BtrI	CACGTC	2 cut(s)	2758, 2955
BtsI	GCAGTG	2 cut(s)	858, 1203
BtsIMutI	CAGTG	9 cut(s)	420, 858, 1203, 1448, 1617, 2361, 2773, 3027, 3259
BveI	ACCTGC	2 cut(s)	510, 2594
CaiI	CAGNNNCTG	5 cut(s)	328, 588, 1319, 2306, 2696
CciI	TCATGA	1 cut(s)	639
CfoI	GCGC	4 cut(s)	99, 449, 1384, 1898
Cfr10I	RCCGGY	2 cut(s)	1097, 2277
Cfr13I	GGNCC	5 cut(s)	43, 331, 531, 826, 1697
CseI	GACGC	3 cut(s)	41, 665, 1015
Csp6I	GTAC	4 cut(s)	301, 1131, 1719, 2740
CspAI	ACCGGT	1 cut(s)	2277
CspCI	CAANNNNNGTGG	2 cut(s)	3191, 3226
CviQI	GTAC	4 cut(s)	301, 1131, 1719, 2740
DraI	TTTAAA	1 cut(s)	3004
EaeI	YGGCCR	6 cut(s)	565, 739, 911, 1623, 1653, 2681
Eam1104I	CTCTTC	4 cut(s)	214, 1798, 3042, 3156
EarI	CTCTTC	4 cut(s)	214, 1798, 3042, 3156
EciI	GGCGGA	3 cut(s)	51, 97, 591
Ecl136II	GAGCTC	1 cut(s)	2339
Eco105I	TACGTA	1 cut(s)	1718
Eco130I	CCWWGG	6 cut(s)	849, 914, 1660, 2542, 3174, 3259
Eco147I	AGGCCT	2 cut(s)	1988, 2377
Eco24I	GRGCYC	2 cut(s)	1841, 2341
Eco31I	GGTCTC	2 cut(s)	481, 3151
Eco47I	GGWCC	3 cut(s)	331, 531, 1697
Eco47III	AGCGCT	1 cut(s)	1383
Eco53kI	GAGCTC	1 cut(s)	2339
Eco57I	CTGAAG	3 cut(s)	2385, 2409, 3408
Eco81I	CCTNAGG	3 cut(s)	527, 951, 1001
Eco88I	CYCGRG	2 cut(s)	2334, 3064
Eco91I	GGTNACC	1 cut(s)	1457
EcoICRI	GAGCTC	1 cut(s)	2339
EcoO109I	RGGNCCY	1 cut(s)	1697
EcoO65I	GGTNACC	1 cut(s)	1457
EcoRI	GAATTC	1 cut(s)	398
EcoRII	CCWGG	8 cut(s)	460, 567, 613, 818, 1115, 1693, 2648, 3363
EcoT14I	CCWWGG	6 cut(s)	849, 914, 1660, 2542, 3174, 3259
EcoT38I	GRGCYC	2 cut(s)	1841, 2341
ErhI	CCWWGG	6 cut(s)	849, 914, 1660, 2542, 3174, 3259
FalI	AAGNNNNNCTT	2 cut(s)	590, 622
FaqI	GGGAC	4 cut(s)	44, 453, 1531, 2474
FauI	CCCGC	4 cut(s)	34, 74, 3233, 3335
FauNDI	CATATG	1 cut(s)	963
FbaI	TGATCA	3 cut(s)	2248, 2770, 3120
FriOI	GRGCYC	2 cut(s)	1841, 2341
FspBI	CTAG	3 cut(s)	267, 2025, 3435
GlaI	GCGC	4 cut(s)	98, 448, 1383, 1897
GsaI	CCCAGC	2 cut(s)	556, 1350
GsuI	CTGGAG	2 cut(s)	1677, 2121
HaeII	RGCGCY	1 cut(s)	1385
HapII	CCGG	6 cut(s)	1098, 1269, 1850, 2278, 2529, 2785
HgaI	GACGC	3 cut(s)	41, 665, 1015
HhaI	GCGC	4 cut(s)	99, 449, 1384, 1898
Hin1I	GRCGYC	4 cut(s)	153, 603, 657, 1026
Hin6I	GCGC	4 cut(s)	97, 447, 1382, 1896
HinP1I	GCGC	4 cut(s)	97, 447, 1382, 1896
HincII	GTYRAC	2 cut(s)	682, 1186
HindII	GTYRAC	2 cut(s)	682, 1186
HpaII	CCGG	6 cut(s)	1098, 1269, 1850, 2278, 2529, 2785
HphI	GGTGA	7 cut(s)	26, 368, 659, 881, 1472, 2426, 2639
Hpy99I	CGWCG	5 cut(s)	26, 152, 155, 221, 1329
HpyAV	CCTTC	9 cut(s)	592, 803, 1120, 1157, 1225, 1937, 1991, 2948, 3011
HpyCH4IV	ACGT	8 cut(s)	153, 159, 303, 603, 1717, 2014, 2757, 2954
HpySE526I	ACGT	8 cut(s)	153, 159, 303, 603, 1717, 2014, 2757, 2954
Hsp92I	GRCGYC	4 cut(s)	153, 603, 657, 1026
HspAI	GCGC	4 cut(s)	97, 447, 1382, 1896
Ksp22I	TGATCA	3 cut(s)	2248, 2770, 3120
LguI	GCTCTTC	2 cut(s)	1798, 3042
LmnI	GCTCC	7 cut(s)	481, 841, 1005, 1307, 1413, 2102, 2344
MaeI	CTAG	3 cut(s)	267, 2025, 3435
MaeII	ACGT	8 cut(s)	153, 159, 303, 603, 1717, 2014, 2757, 2954
MaeIII	GTNAC	7 cut(s)	1012, 1457, 1875, 2135, 2672, 2758, 3287
MbiI	CCGCTC	1 cut(s)	1416
MflI	RGATCY	1 cut(s)	1564
MhlI	GDGCHC	4 cut(s)	838, 1841, 2341, 2717
MlsI	TGGCCA	4 cut(s)	567, 741, 913, 1655
MluNI	TGGCCA	4 cut(s)	567, 741, 913, 1655
MlyI	GAGTC	5 cut(s)	200, 588, 645, 2175, 3329
MmeI	TCCRAC	5 cut(s)	267, 464, 1609, 2600, 3369
Mox20I	TGGCCA	4 cut(s)	567, 741, 913, 1655
MroXI	GAANNNNTTC	2 cut(s)	2537, 3165
MscI	TGGCCA	4 cut(s)	567, 741, 913, 1655
MseI	TTAA	9 cut(s)	177, 804, 1020, 1155, 1476, 2325, 3003, 3245, 3425
MslI	CAYNNNNRTG	4 cut(s)	747, 2476, 2756, 3245
Msp20I	TGGCCA	4 cut(s)	567, 741, 913, 1655
MspA1I	CMGCKG	4 cut(s)	94, 411, 556, 2303
MspI	CCGG	6 cut(s)	1098, 1269, 1850, 2278, 2529, 2785
Mva1269I	GAATGC	2 cut(s)	386, 2640
MvaI	CCWGG	8 cut(s)	462, 569, 615, 820, 1117, 1695, 2650, 3365
MvnI	CGCG	4 cut(s)	447, 1291, 1674, 3228
NciI	CCSGG	2 cut(s)	1270, 2530
NcoI	CCATGG	3 cut(s)	849, 914, 1660
NdeI	CATATG	1 cut(s)	963
NlaIV	GGNNCC	6 cut(s)	438, 524, 886, 1007, 2597, 3073
NmuCI	GTSAC	3 cut(s)	2672, 2758, 3287
NspI	RCATGY	3 cut(s)	1192, 2084, 3285
NspV	TTCGAA	1 cut(s)	2010
PaeR7I	CTCGAG	1 cut(s)	2334
PagI	TCATGA	1 cut(s)	639
PceI	AGGCCT	2 cut(s)	1988, 2377
PciI	ACATGT	3 cut(s)	1188, 2080, 3281
PciSI	GCTCTTC	2 cut(s)	1798, 3042
PcsI	WCGNNNNNNNCGW	2 cut(s)	156, 300
PctI	GAATGC	2 cut(s)	386, 2640
PdmI	GAANNNNTTC	2 cut(s)	2537, 3165
PfeI	GAWTC	9 cut(s)	674, 1177, 1364, 1552, 2068, 2160, 2568, 2621, 2891
PflFI	GACNNNGTC	1 cut(s)	152
PflMI	CCANNNNNTGG	1 cut(s)	328
PfoI	TCCNGGA	1 cut(s)	1693
PinAI	ACCGGT	1 cut(s)	2277
PleI	GAGTC	5 cut(s)	200, 587, 644, 2174, 3329
PpsI	GAGTC	5 cut(s)	200, 587, 644, 2174, 3329
Ppu21I	YACGTR	2 cut(s)	304, 1718
PpuMI	RGGWCCY	1 cut(s)	1697
PscI	ACATGT	3 cut(s)	1188, 2080, 3281
PshAI	GACNNNNGTC	1 cut(s)	512
Psp124BI	GAGCTC	1 cut(s)	2341
Psp5II	RGGWCCY	1 cut(s)	1697
Psp6I	CCWGG	8 cut(s)	460, 567, 613, 818, 1115, 1693, 2648, 3363
PspEI	GGTNACC	1 cut(s)	1457
PspFI	CCCAGC	2 cut(s)	552, 1346
PspGI	CCWGG	8 cut(s)	460, 567, 613, 818, 1115, 1693, 2648, 3363
PspN4I	GGNNCC	6 cut(s)	438, 524, 886, 1007, 2597, 3073
PspPI	GGNCC	5 cut(s)	43, 331, 531, 826, 1697
PspPPI	RGGWCCY	1 cut(s)	1697
PstI	CTGCAG	2 cut(s)	1315, 1921
PstNI	CAGNNNCTG	5 cut(s)	328, 588, 1319, 2306, 2696
PsuI	RGATCY	1 cut(s)	1564
PsyI	GACNNNGTC	1 cut(s)	152
PvuII	CAGCTG	3 cut(s)	94, 556, 2303
RsaI	GTAC	4 cut(s)	302, 1132, 1720, 2741
RsaNI	GTAC	4 cut(s)	301, 1131, 1719, 2740
RseI	CAYNNNNRTG	4 cut(s)	747, 2476, 2756, 3245
SacI	GAGCTC	1 cut(s)	2341
SapI	GCTCTTC	2 cut(s)	1798, 3042
SaqAI	TTAA	9 cut(s)	177, 804, 1020, 1155, 1476, 2325, 3003, 3245, 3425
Sau96I	GGNCC	5 cut(s)	43, 331, 531, 826, 1697
SchI	GAGTC	5 cut(s)	200, 588, 645, 2175, 3329
SduI	GDGCHC	4 cut(s)	838, 1841, 2341, 2717
SfcI	CTRYAG	6 cut(s)	1030, 1311, 1701, 1917, 1932, 2718
Sfr274I	CTCGAG	1 cut(s)	2334
SfuI	TTCGAA	1 cut(s)	2010
SinI	GGWCC	3 cut(s)	331, 531, 1697
SlaI	CTCGAG	1 cut(s)	2334
SmiMI	CAYNNNNRTG	4 cut(s)	747, 2476, 2756, 3245
SmlI	CTYRAG	4 cut(s)	1044, 1568, 2334, 3095
SmoI	CTYRAG	4 cut(s)	1044, 1568, 2334, 3095
SnaBI	TACGTA	1 cut(s)	1718
SpeI	ACTAGT	1 cut(s)	266
SseBI	AGGCCT	2 cut(s)	1988, 2377
SspMI	CTAG	3 cut(s)	267, 2025, 3435
SstI	GAGCTC	1 cut(s)	2341
StuI	AGGCCT	2 cut(s)	1988, 2377
StyI	CCWWGG	6 cut(s)	849, 914, 1660, 2542, 3174, 3259
TaiI	ACGT	8 cut(s)	156, 162, 306, 606, 1720, 2017, 2760, 2957
TaqI	TCGA	8 cut(s)	147, 1180, 1324, 2010, 2260, 2335, 3088, 3298
TauI	GCSGC	6 cut(s)	312, 414, 417, 420, 662, 3307
TfiI	GAWTC	9 cut(s)	674, 1177, 1364, 1552, 2068, 2160, 2568, 2621, 2891
Tru1I	TTAA	9 cut(s)	177, 804, 1020, 1155, 1476, 2325, 3003, 3245, 3425
Tru9I	TTAA	9 cut(s)	177, 804, 1020, 1155, 1476, 2325, 3003, 3245, 3425
TscAI	CASTG	9 cut(s)	427, 865, 1203, 1448, 1617, 2368, 2773, 3034, 3259
TseFI	GTSAC	3 cut(s)	2672, 2758, 3287
Tsp45I	GTSAC	3 cut(s)	2672, 2758, 3287
TspDTI	ATGAA	7 cut(s)	69, 391, 1244, 2466, 2537, 3143, 3158
TspGWI	ACGGA	3 cut(s)	442, 667, 2100
TspRI	CASTG	9 cut(s)	427, 865, 1203, 1448, 1617, 2368, 2773, 3034, 3259
Tth111I	GACNNNGTC	1 cut(s)	152
Van91I	CCANNNNNTGG	1 cut(s)	328
VpaK11BI	GGWCC	3 cut(s)	331, 531, 1697
XapI	RAATTY	5 cut(s)	398, 592, 1238, 1770, 3182
XceI	RCATGY	3 cut(s)	1192, 2084, 3285
XcmI	CCANNNNNNNNNTGG	2 cut(s)	560, 3328
XhoI	CTCGAG	1 cut(s)	2334
XmnI	GAANNNNTTC	2 cut(s)	2537, 3165
XspI	CTAG	3 cut(s)	267, 2025, 3435
ZraI	GACGTC	2 cut(s)	154, 604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.