MD00G1094900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015348
Physical Location & Seq
Reverse (-)
19804363 .. 19807099
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1094900.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGATGCAAGTATGTTTGCGGAGTAAAGAAATTGGTAAGGCGATGAGAGTGTGCAGCAGGTGGCGCCGTTTCCTTATCTTTCTACCTCTTATTTTCATTCTTGCTCACTTCTTGTCCGTTAAGGAATTGCATCATAACTCAGCTGTGGAAGATTTGCCCAGGAAGAAAGAAAGCAACAAATCCAAATCCAACCACTTGGTTCTTGGCCCTGCTGCTGGCCAAGGCTTGAGCAATCGCCTCCAATGCCATGGAGATGGCAATGGCATCAAAGCTCTCAACCACACCACCCATTCTTCCCGTGCCTCCGCTGCGGTTGACAGCATTGCTGTTGTCACTGTCTTCACCATATACAATTCTTCCACCTCCAATGGGCACGTCGAAGATCAATTCTCAAACTCGGTTACAGTTGGGAATGCTTCTTATACTAAATTCGAGAGATCCATGGCCGTCTTGAATGTCTTTATTAACTTCATTCAGCTGGCTATGCCCCGGAGCAGTGTAGTCATTCTAACAGACCCAGCAGCTGATCTTTCAATACGCAGAAACAAGGTCACTGTATATCCAATTCAAGGTGAATATTCACGAGACAAGTTGATGCTTCAGAGAATCAGGTCTTACATTGCCTTCCTAGAATCTAGGATGGAGGAGCTTCCTCAGAATCAGAAGCATACTCATTACATCTTCACCGACTCAGATATCACTGTTGTTGATGATCTAGGACACATATTTAGTGAATATCCGAATTTTCACCTGGCACTTACGTTTCGCAATAACAAAGCACAGCCTCTGAATTCAGGATTTATAGCAGTGAGGGGCACCGATGATGGAATTTTAAGGGCAAAGATTTTTCTACAAAAAGTACTAAAAGTTTATCAAACAAGGTACATGAAGGCTTCTAGAATGCTTGGGGATCAATTAGCTCTTGCCTGGGTAGTGACGTCACACTCATCTTTCGATGCAAAGAAATTTACAAAAGCCAAAGCTTTCGTAGATGATATTGGCGGTGCTTCCATGTTGTTTTTACCCTGTGCTGTGTACAACTGGACACCACCTGAAGGCGCTGGCCAATTTCATGGCATGCCCTTGGATGTAAAGGTTGTTCATTTCAAAGGATCAAGGAAACGCCTGATGCTCGAGTCTTGGAACTTATACAGTTCCGTTTCCAACATTCCAGATATGTTGTGCCTGATATTAAAGAGTGGGAGAACAAAGTACGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

408

Amino Acids

45.83

Weight (kDa)

9.65

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45320
fragaria_vesca FvH4_1g28100 FvH4_1g28100
malus_domestica MD00G1094900.v1.1
prunus_persica Prupe.6G155400_v2.0.a1
pyrus_communis pycom5153684g00430
rosa_chinensis RchiOBHm_Chr3g0497141
rosa_laevigata RLG00000022478
rosa_multiflora Rmu_sc0002893.1_g000029
rosa_roxburghii Rroxscaffold_6G00388380
rosa_rugosa Rorug03G0291300
rosa_samantha Rh3AG334600 Rh3BG371700 Rh3CG368400 Rh3DG369400
rosa_wichuraiana Rw3G029360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 48
AatII GACGTC 1 cut(s) 941
Acc36I ACCTGC 1 cut(s) 48
AccB1I GGYRCC 2 cut(s) 63, 815
AciI CCGC 4 cut(s) 19, 306, 311, 1002
AclWI GGATC 3 cut(s) 432, 918, 1120
AcoI YGGCCR 3 cut(s) 217, 444, 1063
AcsI RAATTY 5 cut(s) 428, 742, 790, 828, 965
AcuI CTGAAG 2 cut(s) 584, 1074
AcyI GRCGYC 2 cut(s) 64, 938
AfaI GTAC 4 cut(s) 861, 884, 1037, 1214
AfiI CCNNNNNNNGG 4 cut(s) 215, 369, 569, 1055
AgsI TTSAA 4 cut(s) 454, 534, 569, 1108
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 3 cut(s) 158, 750, 926
AluBI AGCT 7 cut(s) 143, 272, 478, 524, 649, 920, 983
AluI AGCT 7 cut(s) 143, 272, 478, 524, 649, 920, 983
Alw26I GTCTC 1 cut(s) 579
AlwI GGATC 3 cut(s) 432, 918, 1120
AlwNI CAGNNNCTG 2 cut(s) 524, 787
Ama87I CYCGRG 1 cut(s) 1133
AoxI GGCC 4 cut(s) 205, 217, 444, 1063
ApeKI GCWGC 4 cut(s) 54, 212, 308, 521
ApoI RAATTY 5 cut(s) 428, 742, 790, 828, 965
AspLEI GCGC 2 cut(s) 66, 1061
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 1 cut(s) 490
AsuHPI GGTGA 4 cut(s) 334, 584, 676, 740
AvaI CYCGRG 1 cut(s) 1133
BaeGI GKGCMC 2 cut(s) 375, 818
BalI TGGCCA 2 cut(s) 219, 1065
BanI GGYRCC 2 cut(s) 63, 815
BauI CACGAG 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 331
BbvI GCAGC 4 cut(s) 66, 199, 295, 533
BccI CCATC 3 cut(s) 248, 634, 818
BceAI ACGGC 2 cut(s) 51, 431
BciT130I CCWGG 3 cut(s) 160, 752, 928
BcnI CCSGG 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 579
BfaI CTAG 4 cut(s) 629, 636, 716, 897
BfoI RGCGCY 2 cut(s) 67, 1062
BfuAI ACCTGC 1 cut(s) 48
BisI GCNGC 4 cut(s) 55, 213, 309, 522
BlsI GCNGC 4 cut(s) 56, 214, 310, 523
BmcAI AGTACT 1 cut(s) 861
Bme1390I CCNGG 4 cut(s) 160, 490, 752, 928
BmeT110I CYCGRG 1 cut(s) 1133
BmgBI CACGTC 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 65, 817
BmrFI CCNGG 4 cut(s) 160, 490, 752, 928
BmsI GCATC 5 cut(s) 139, 273, 585, 946, 1119
BpiI GAAGAC 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 247
BpuMI CCSGG 1 cut(s) 490
BsaHI GRCGYC 2 cut(s) 64, 938
BsaJI CCNNGG 7 cut(s) 158, 220, 247, 441, 488, 927, 1083
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 369, 569, 1055
Bse1I ACTGG 1 cut(s) 1046
Bse3DI GCAATG 3 cut(s) 265, 321, 618
BseBI CCWGG 3 cut(s) 160, 752, 928
BseDI CCNNGG 7 cut(s) 158, 220, 247, 441, 488, 927, 1083
BseGI GGATG 2 cut(s) 645, 1093
BseLI CCNNNNNNNGG 4 cut(s) 215, 369, 569, 1055
BseMI GCAATG 3 cut(s) 265, 321, 618
BseMII CTCAG 3 cut(s) 153, 668, 705
BseNI ACTGG 1 cut(s) 1046
BseRI GAGGAG 1 cut(s) 659
BseSI GKGCMC 2 cut(s) 375, 818
BseXI GCAGC 4 cut(s) 66, 199, 295, 533
BseYI CCCAGC 1 cut(s) 517
BsgI GTGCAG 1 cut(s) 73
BshFI GGCC 4 cut(s) 207, 219, 446, 1065
BshNI GGYRCC 2 cut(s) 63, 815
BsiHKCI CYCGRG 1 cut(s) 1133
BsiSI CCGG 1 cut(s) 490
BslI CCNNNNNNNGG 4 cut(s) 215, 369, 569, 1055
BsmAI GTCTC 1 cut(s) 579
BsmI GAATGC 2 cut(s) 418, 906
BsnI GGCC 4 cut(s) 207, 219, 446, 1065
BsoBI CYCGRG 1 cut(s) 1133
Bsp1286I GDGCHC 2 cut(s) 375, 818
Bsp1407I TGTACA 1 cut(s) 1035
Bsp143I GATC 6 cut(s) 382, 437, 526, 712, 910, 1112
Bsp19I CCATGG 2 cut(s) 247, 441
BspACI CCGC 4 cut(s) 19, 306, 311, 1002
BspANI GGCC 4 cut(s) 207, 219, 446, 1065
BspCNI CTCAG 3 cut(s) 152, 667, 704
BspLI GGNNCC 2 cut(s) 65, 817
BspMI ACCTGC 1 cut(s) 48
BspPI GGATC 3 cut(s) 432, 918, 1120
BspT107I GGYRCC 2 cut(s) 63, 815
BsrDI GCAATG 3 cut(s) 265, 321, 618
BsrGI TGTACA 1 cut(s) 1035
BsrI ACTGG 1 cut(s) 1046
BssECI CCNNGG 7 cut(s) 158, 220, 247, 441, 488, 927, 1083
BssMI GATC 6 cut(s) 382, 437, 526, 712, 910, 1112
BssNI GRCGYC 2 cut(s) 64, 938
BssSI CACGAG 1 cut(s) 582
BssT1I CCWWGG 4 cut(s) 220, 247, 441, 1083
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 3 cut(s) 160, 752, 928
Bst4CI ACNGT 5 cut(s) 337, 406, 556, 703, 1154
BstACI GRCGYC 2 cut(s) 64, 938
BstAUI TGTACA 1 cut(s) 1035
BstC8I GCNNGC 4 cut(s) 217, 480, 1063, 1079
BstDEI CTNAG 3 cut(s) 139, 654, 691
BstDSI CCRYGG 2 cut(s) 247, 441
BstF5I GGATG 2 cut(s) 645, 1093
BstH2I RGCGCY 2 cut(s) 67, 1062
BstHHI GCGC 2 cut(s) 66, 1061
BstKTI GATC 6 cut(s) 385, 440, 529, 715, 913, 1115
BstMAI GTCTC 1 cut(s) 579
BstMBI GATC 6 cut(s) 382, 437, 526, 712, 910, 1112
BstMWI GCNNNNNNNGC 4 cut(s) 63, 243, 308, 484
BstNI CCWGG 3 cut(s) 160, 752, 928
BstNSI RCATGY 1 cut(s) 1081
BstSCI CCNGG 4 cut(s) 158, 488, 750, 926
BstSLI GKGCMC 2 cut(s) 375, 818
BstV1I GCAGC 4 cut(s) 66, 199, 295, 533
BstV2I GAAGAC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 437
BstXI CCANNNNNNTGG 4 cut(s) 196, 248, 254, 1073
BstYI RGATCY 1 cut(s) 437
BsuRI GGCC 4 cut(s) 207, 219, 446, 1065
BtgI CCRYGG 2 cut(s) 247, 441
BtgZI GCGATG 1 cut(s) 56
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 2 cut(s) 645, 1093
BtsI GCAGTG 2 cut(s) 502, 813
BtsIMutI CAGTG 5 cut(s) 333, 502, 552, 699, 813
BveI ACCTGC 1 cut(s) 48
Cac8I GCNNGC 4 cut(s) 217, 480, 1063, 1079
CaiI CAGNNNCTG 2 cut(s) 524, 787
CfoI GCGC 2 cut(s) 66, 1061
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 4 cut(s) 860, 883, 1036, 1213
CviAII CATG 6 cut(s) 248, 442, 886, 1012, 1073, 1078
CviQI GTAC 4 cut(s) 860, 883, 1036, 1213
DdeI CTNAG 3 cut(s) 139, 654, 691
DinI GGCGCC 1 cut(s) 65
DpnI GATC 6 cut(s) 384, 439, 528, 714, 912, 1114
DpnII GATC 6 cut(s) 382, 437, 526, 712, 910, 1112
EaeI YGGCCR 3 cut(s) 217, 444, 1063
Eco130I CCWWGG 4 cut(s) 220, 247, 441, 1083
Eco32I GATATC 1 cut(s) 697
Eco57I CTGAAG 2 cut(s) 584, 1074
Eco88I CYCGRG 1 cut(s) 1133
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 3 cut(s) 158, 750, 926
EcoRV GATATC 1 cut(s) 697
EcoT14I CCWWGG 4 cut(s) 220, 247, 441, 1083
EgeI GGCGCC 1 cut(s) 65
EheI GGCGCC 1 cut(s) 65
ErhI CCWWGG 4 cut(s) 220, 247, 441, 1083
FaeI CATG 6 cut(s) 251, 445, 889, 1015, 1076, 1081
FatI CATG 6 cut(s) 247, 441, 885, 1011, 1072, 1077
Fnu4HI GCNGC 4 cut(s) 55, 213, 309, 522
FokI GGATG 2 cut(s) 652, 1100
Fsp4HI GCNGC 4 cut(s) 55, 213, 309, 522
FspBI CTAG 4 cut(s) 629, 636, 716, 897
GlaI GCGC 2 cut(s) 65, 1060
GluI GCNGC 4 cut(s) 55, 213, 309, 522
GsaI CCCAGC 1 cut(s) 521
HaeII RGCGCY 2 cut(s) 67, 1062
HaeIII GGCC 4 cut(s) 207, 219, 446, 1065
HapII CCGG 1 cut(s) 490
HhaI GCGC 2 cut(s) 66, 1061
Hin1I GRCGYC 2 cut(s) 64, 938
Hin1II CATG 6 cut(s) 251, 445, 889, 1015, 1076, 1081
Hin6I GCGC 2 cut(s) 64, 1059
HinP1I GCGC 2 cut(s) 64, 1059
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 981
HinfI GANTC 5 cut(s) 606, 632, 658, 689, 1136
HpaII CCGG 1 cut(s) 490
HphI GGTGA 4 cut(s) 334, 584, 676, 740
Hpy166II GTNNAC 2 cut(s) 316, 1036
Hpy188I TCNGA 6 cut(s) 603, 657, 663, 694, 741, 789
Hpy188III TCNNGA 6 cut(s) 433, 451, 582, 795, 897, 1172
Hpy8I GTNNAC 2 cut(s) 316, 1036
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 3 cut(s) 634, 883, 1049
HpyCH4III ACNGT 5 cut(s) 337, 406, 556, 703, 1154
HpyCH4IV ACGT 3 cut(s) 375, 761, 938
HpyCH4V TGCA 4 cut(s) 7, 54, 130, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 243, 308, 484
HpyF3I CTNAG 3 cut(s) 139, 654, 691
HpySE526I ACGT 3 cut(s) 375, 761, 938
Hsp92I GRCGYC 2 cut(s) 64, 938
Hsp92II CATG 6 cut(s) 251, 445, 889, 1015, 1076, 1081
HspAI GCGC 2 cut(s) 64, 1059
KasI GGCGCC 1 cut(s) 63
Kzo9I GATC 6 cut(s) 382, 437, 526, 712, 910, 1112
LmnI GCTCC 2 cut(s) 492, 646
Lsp1109I GCAGC 4 cut(s) 66, 199, 295, 533
LweI GCATC 5 cut(s) 139, 273, 585, 946, 1119
MaeI CTAG 4 cut(s) 629, 636, 716, 897
MaeII ACGT 3 cut(s) 375, 761, 938
MaeIII GTNAC 5 cut(s) 331, 400, 550, 934, 939
MalI GATC 6 cut(s) 384, 439, 528, 714, 912, 1114
MboI GATC 6 cut(s) 382, 437, 526, 712, 910, 1112
MboII GAAGA 7 cut(s) 161, 175, 285, 331, 348, 392, 673
MflI RGATCY 1 cut(s) 437
MhlI GDGCHC 2 cut(s) 375, 818
MlsI TGGCCA 2 cut(s) 219, 1065
MluNI TGGCCA 2 cut(s) 219, 1065
Mly113I GGCGCC 1 cut(s) 64
MlyI GAGTC 2 cut(s) 683, 1145
MmeI TCCRAC 2 cut(s) 213, 1188
MnlI CCTC 8 cut(s) 96, 248, 313, 373, 637, 663, 795, 804
Mox20I TGGCCA 2 cut(s) 219, 1065
MscI TGGCCA 2 cut(s) 219, 1065
MseI TTAA 4 cut(s) 120, 465, 833, 1193
MslI CAYNNNNRTG 1 cut(s) 252
Msp20I TGGCCA 2 cut(s) 219, 1065
MspA1I CMGCKG 4 cut(s) 143, 308, 478, 524
MspI CCGG 1 cut(s) 490
MspR9I CCNGG 4 cut(s) 160, 490, 752, 928
Mva1269I GAATGC 2 cut(s) 418, 906
MvaI CCWGG 3 cut(s) 160, 752, 928
MwoI GCNNNNNNNGC 4 cut(s) 63, 243, 308, 484
NarI GGCGCC 1 cut(s) 64
NciI CCSGG 1 cut(s) 490
NcoI CCATGG 2 cut(s) 247, 441
NdeII GATC 6 cut(s) 382, 437, 526, 712, 910, 1112
NlaIII CATG 6 cut(s) 251, 445, 889, 1015, 1076, 1081
NlaIV GGNNCC 2 cut(s) 65, 817
NmuCI GTSAC 4 cut(s) 331, 550, 934, 939
NspI RCATGY 1 cut(s) 1081
PaeI GCATGC 1 cut(s) 1081
PaeR7I CTCGAG 1 cut(s) 1133
PaqCI CACCTGC 1 cut(s) 48
PctI GAATGC 2 cut(s) 418, 906
PfeI GAWTC 3 cut(s) 606, 632, 658
PkrI GCNGC 4 cut(s) 56, 214, 310, 523
PleI GAGTC 2 cut(s) 683, 1144
PluTI GGCGCC 1 cut(s) 67
PpsI GAGTC 2 cut(s) 683, 1144
Psp6I CCWGG 3 cut(s) 158, 750, 926
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 3 cut(s) 158, 750, 926
PspN4I GGNNCC 2 cut(s) 65, 817
PspPI GGNCC 1 cut(s) 206
PspXI VCTCGAGB 1 cut(s) 1133
PsrI GAACNNNNNNTAC 2 cut(s) 1083, 1115
PstNI CAGNNNCTG 2 cut(s) 524, 787
PsuI RGATCY 1 cut(s) 437
PvuII CAGCTG 3 cut(s) 143, 478, 524
RsaI GTAC 4 cut(s) 861, 884, 1037, 1214
RsaNI GTAC 4 cut(s) 860, 883, 1036, 1213
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 4 cut(s) 120, 465, 833, 1193
SatI GCNGC 4 cut(s) 55, 213, 309, 522
Sau3AI GATC 6 cut(s) 382, 437, 526, 712, 910, 1112
Sau96I GGNCC 1 cut(s) 206
ScaI AGTACT 1 cut(s) 861
SchI GAGTC 2 cut(s) 683, 1145
ScrFI CCNGG 4 cut(s) 160, 490, 752, 928
SduI GDGCHC 2 cut(s) 375, 818
SfaNI GCATC 5 cut(s) 139, 273, 585, 946, 1119
SfoI GGCGCC 1 cut(s) 65
Sfr274I CTCGAG 1 cut(s) 1133
SlaI CTCGAG 1 cut(s) 1133
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 2 cut(s) 226, 1133
SmoI CTYRAG 2 cut(s) 226, 1133
SphI GCATGC 1 cut(s) 1081
SsiI CCGC 4 cut(s) 19, 306, 311, 1002
SspDI GGCGCC 1 cut(s) 63
SspI AATATT 1 cut(s) 578
SspMI CTAG 4 cut(s) 629, 636, 716, 897
StyD4I CCNGG 4 cut(s) 158, 488, 750, 926
StyI CCWWGG 4 cut(s) 220, 247, 441, 1083
TaaI ACNGT 5 cut(s) 337, 406, 556, 703, 1154
TaiI ACGT 3 cut(s) 378, 764, 941
TaqI TCGA 4 cut(s) 378, 432, 954, 1134
TatI WGTACW 2 cut(s) 859, 1035
TfiI GAWTC 3 cut(s) 606, 632, 658
Tru1I TTAA 4 cut(s) 120, 465, 833, 1193
Tru9I TTAA 4 cut(s) 120, 465, 833, 1193
TscAI CASTG 5 cut(s) 340, 502, 559, 706, 813
TseFI GTSAC 4 cut(s) 331, 550, 934, 939
TseI GCWGC 4 cut(s) 54, 212, 308, 521
Tsp45I GTSAC 4 cut(s) 331, 550, 934, 939
TspDTI ATGAA 5 cut(s) 85, 460, 902, 1061, 1091
TspGWI ACGGA 2 cut(s) 106, 1147
TspRI CASTG 5 cut(s) 340, 502, 559, 706, 813
XapI RAATTY 5 cut(s) 428, 742, 790, 828, 965
XbaI TCTAGA 1 cut(s) 896
XceI RCATGY 1 cut(s) 1081
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XhoI CTCGAG 1 cut(s) 1133
XspI CTAG 4 cut(s) 629, 636, 716, 897
ZraI GACGTC 1 cut(s) 939
ZrmI AGTACT 1 cut(s) 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.