RLG00000022478

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015348
Physical Location & Seq
Forward (+)
1207366 .. 1210471
3106 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022478

Sequence Viewer

Length: 1149 bp
ATGAGGACCATAAGTGGATGGCGTCGCTTCCTCATCCTCGTCCCTCTTATTTTCTTTCTTGCTCACTTCTTCTCTGTTGATCAATTGCATTACAACCCACGACCTGTGCCCAACAAAGCAAGCAAATTCAACCACTTGGTTCTCGGACCTGCTGCCGGCCAAGGCTTGACCAATCGTCTCCAATGCCAACCCCAAGGAATCAAAGCTCTTAACAAAACCCATTTCTCCACTGCCACTGCCCCTGCCCTTGATAACATTCACTTTGTCACCGTCTTCACCCTATATAATTCCACCCGTCCCGATGATCAGTTATCAAACTTGGTTACAGTTGGCAATGCTTCTTACACTAAATTGGAGAGGTCTATGGCCATTCTCAATATTTTCGTTAACTTCATCCAGGTGGCAATGCCCCGGAGCAATGTAGTGATTTTAACTGACCCAGCATCTGATCTTTCAATGCACAGAAATGGGGTCACCATATATCCAATTCAAGGTGAATATTCGCGAGACAAGTTGATGCTTCAAAGAATCAGGTCTTACATTGCCTTCCTAGAAACAAGGATGGAGGAGGTTTCTCAAAACCAAGGTCATATTCATCATTACATCTTCACCGACTCAGATATAGTCGTGGTTGATGATCTAGGACATATATTTCATGATTATCCAAATTTTCATCTGGCACTGACCTTCCGGAACAACAAAGAACAACCTCTGAATTCAGGATTTATAGCAGTCAGGGGTACTCCTGCTGGCATTTTAAGGGCAAAGACTTTTCTACAAGAAGTACTGAAAGTTTACAGGTCAAGGTATATGAAAGCTTCACGGATGCTTGGGGATCAGTTGGCCCTTGCCTGGGTGGTCAAGTCACACCCATCTTTTGATCCAAAGAAGTTCACTAAAGCCCAAGCTTTCATAGAAGATATTGACGGTGCTGCAGTGTTATTTTTACCCTGTGCTGTATACAATTGGACACCACCAGAGGGTGCAGGTCAATTTCATGGCATGCCTTTGGATGTTAAGGTCATTCATTTCAAAGGATCAAGGAAACGACTAATGGTCGAATCATGGAACTTCTTCAGTGCATCTTCTGACATTTCAGATATGTTGTGTCTGATATTGAAGAGTGGGAGAACGAAGTACGACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

383

Amino Acids

43.37

Weight (kDa)

9.51

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45320
fragaria_vesca FvH4_1g28100 FvH4_1g28100
malus_domestica MD00G1094900.v1.1
prunus_persica Prupe.6G155400_v2.0.a1
pyrus_communis pycom5153684g00430
rosa_chinensis RchiOBHm_Chr3g0497141
rosa_laevigata RLG00000022478
rosa_multiflora Rmu_sc0002893.1_g000029
rosa_roxburghii Rroxscaffold_6G00388380
rosa_rugosa Rorug03G0291300
rosa_samantha Rh3AG334600 Rh3BG371700 Rh3CG368400 Rh3DG369400
rosa_wichuraiana Rw3G029360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 157, 977
AccI GTMKAC 1 cut(s) 960
AccII CGCG 1 cut(s) 505
AccIII TCCGGA 1 cut(s) 690
AclWI GGATC 3 cut(s) 843, 875, 1045
AcoI YGGCCR 2 cut(s) 157, 366
AcsI RAATTY 3 cut(s) 125, 667, 715
AcuI CTGAAG 1 cut(s) 1060
AcyI GRCGYC 1 cut(s) 22
AfaI GTAC 3 cut(s) 742, 786, 1139
AfiI CCNNNNNNNGG 5 cut(s) 155, 491, 852, 853, 980
AgsI TTSAA 6 cut(s) 130, 456, 491, 524, 1033, 1120
AhdI GACNNNNNGTC 2 cut(s) 174, 1055
AjnI CCWGG 2 cut(s) 396, 851
AjuI GAANNNNNNNTTGG 2 cut(s) 576, 608
AluBI AGCT 3 cut(s) 206, 818, 908
AluI AGCT 3 cut(s) 206, 818, 908
Alw26I GTCTC 2 cut(s) 182, 501
AlwI GGATC 3 cut(s) 843, 875, 1045
AlwNI CAGNNNCTG 1 cut(s) 446
Aor13HI TCCGGA 1 cut(s) 690
AoxI GGCC 3 cut(s) 157, 366, 843
ApeKI GCWGC 2 cut(s) 152, 932
ApoI RAATTY 3 cut(s) 125, 667, 715
ArsI GACNNNNNNTTYG 2 cut(s) 571, 603
Asp700I GAANNNNTTC 1 cut(s) 1073
AspS9I GGNCC 3 cut(s) 6, 146, 844
AsuC2I CCSGG 1 cut(s) 412
AsuHPI GGTGA 5 cut(s) 259, 268, 466, 506, 601
AvaII GGWCC 2 cut(s) 6, 146
BaeGI GKGCMC 1 cut(s) 111
BalI TGGCCA 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 265
BbvI GCAGC 2 cut(s) 139, 919
BccI CCATC 3 cut(s) 12, 556, 880
BciT130I CCWGG 2 cut(s) 398, 853
BclI TGATCA 2 cut(s) 79, 304
BcnI CCSGG 1 cut(s) 412
BcoDI GTCTC 2 cut(s) 182, 501
BfaI CTAG 2 cut(s) 551, 641
BfmI CTRYAG 1 cut(s) 933
BfuAI ACCTGC 2 cut(s) 157, 977
BisI GCNGC 2 cut(s) 153, 933
BlsI GCNGC 2 cut(s) 154, 934
BmcAI AGTACT 1 cut(s) 786
Bme1390I CCNGG 3 cut(s) 398, 412, 853
Bme18I GGWCC 2 cut(s) 6, 146
BmeRI GACNNNNNGTC 2 cut(s) 174, 1055
BmgT120I GGNCC 3 cut(s) 6, 146, 844
BmrFI CCNGG 3 cut(s) 398, 412, 853
BmsI GCATC 4 cut(s) 452, 507, 816, 1091
BpiI GAAGAC 1 cut(s) 265
BpuMI CCSGG 1 cut(s) 412
BsaHI GRCGYC 1 cut(s) 22
BsaJI CCNNGG 5 cut(s) 160, 193, 410, 583, 852
BsaWI WCCGGW 1 cut(s) 690
Bsc4I CCNNNNNNNGG 5 cut(s) 155, 491, 852, 853, 980
Bse118I RCCGGY 1 cut(s) 155
Bse3DI GCAATG 4 cut(s) 340, 411, 424, 540
BseAI TCCGGA 1 cut(s) 690
BseBI CCWGG 2 cut(s) 398, 853
BseDI CCNNGG 5 cut(s) 160, 193, 410, 583, 852
BseGI GGATG 6 cut(s) 23, 33, 393, 567, 831, 1018
BseLI CCNNNNNNNGG 5 cut(s) 155, 491, 852, 853, 980
BseMI GCAATG 4 cut(s) 340, 411, 424, 540
BseMII CTCAG 1 cut(s) 630
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 2 cut(s) 139, 919
BseYI CCCAGC 1 cut(s) 439
BsgI GTGCAG 1 cut(s) 1005
Bsh1236I CGCG 1 cut(s) 505
BshFI GGCC 3 cut(s) 159, 368, 845
BsiSI CCGG 3 cut(s) 156, 412, 691
BslFI GGGAC 2 cut(s) 26, 282
BslI CCNNNNNNNGG 5 cut(s) 155, 491, 852, 853, 980
BsmAI GTCTC 2 cut(s) 182, 501
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 26, 282
BsnI GGCC 3 cut(s) 159, 368, 845
Bsp1286I GDGCHC 1 cut(s) 111
Bsp13I TCCGGA 1 cut(s) 690
Bsp143I GATC 7 cut(s) 79, 304, 448, 637, 835, 880, 1037
Bsp68I TCGCGA 1 cut(s) 505
BspANI GGCC 3 cut(s) 159, 368, 845
BspCNI CTCAG 1 cut(s) 629
BspEI TCCGGA 1 cut(s) 690
BspFNI CGCG 1 cut(s) 505
BspHI TCATGA 1 cut(s) 655
BspMAI CTGCAG 1 cut(s) 937
BspMI ACCTGC 2 cut(s) 157, 977
BspPI GGATC 3 cut(s) 843, 875, 1045
BsrDI GCAATG 4 cut(s) 340, 411, 424, 540
BsrFI RCCGGY 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 5 cut(s) 160, 193, 410, 583, 852
BssMI GATC 7 cut(s) 79, 304, 448, 637, 835, 880, 1037
BssNAI GTATAC 1 cut(s) 961
BssNI GRCGYC 1 cut(s) 22
BssT1I CCWWGG 3 cut(s) 160, 193, 583
Bst1107I GTATAC 1 cut(s) 961
Bst2UI CCWGG 2 cut(s) 398, 853
Bst4CI ACNGT 3 cut(s) 271, 328, 929
Bst6I CTCTTC 1 cut(s) 1115
BstACI GRCGYC 1 cut(s) 22
BstC8I GCNNGC 4 cut(s) 121, 157, 751, 1004
BstDEI CTNAG 1 cut(s) 616
BstEII GGTNACC 1 cut(s) 472
BstF5I GGATG 6 cut(s) 23, 33, 393, 567, 831, 1018
BstFNI CGCG 1 cut(s) 505
BstKTI GATC 7 cut(s) 82, 307, 451, 640, 838, 883, 1040
BstMAI GTCTC 2 cut(s) 182, 501
BstMBI GATC 7 cut(s) 79, 304, 448, 637, 835, 880, 1037
BstNI CCWGG 2 cut(s) 398, 853
BstNSI RCATGY 1 cut(s) 1006
BstPI GGTNACC 1 cut(s) 472
BstSCI CCNGG 3 cut(s) 396, 410, 851
BstSFI CTRYAG 1 cut(s) 933
BstSLI GKGCMC 1 cut(s) 111
BstUI CGCG 1 cut(s) 505
BstV1I GCAGC 2 cut(s) 139, 919
BstV2I GAAGAC 1 cut(s) 265
BstZ17I GTATAC 1 cut(s) 961
BsuRI GGCC 3 cut(s) 159, 368, 845
BtsCI GGATG 6 cut(s) 23, 33, 393, 567, 831, 1018
BtsI GCAGTG 3 cut(s) 228, 234, 942
BtsIMutI CAGTG 5 cut(s) 228, 234, 680, 942, 1084
BtuMI TCGCGA 1 cut(s) 505
BveI ACCTGC 2 cut(s) 157, 977
Cac8I GCNNGC 4 cut(s) 121, 157, 751, 1004
CaiI CAGNNNCTG 1 cut(s) 446
CciI TCATGA 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 3 cut(s) 6, 146, 844
CseI GACGC 1 cut(s) 11
Csp6I GTAC 3 cut(s) 741, 785, 1138
CviAII CATG 4 cut(s) 656, 998, 1003, 1065
CviJI RGCY 8 cut(s) 159, 165, 206, 368, 818, 845, 902, 908
CviKI_1 RGCY 8 cut(s) 159, 165, 206, 368, 818, 845, 902, 908
CviQI GTAC 3 cut(s) 741, 785, 1138
DdeI CTNAG 1 cut(s) 616
DpnI GATC 7 cut(s) 81, 306, 450, 639, 837, 882, 1039
DpnII GATC 7 cut(s) 79, 304, 448, 637, 835, 880, 1037
DriI GACNNNNNGTC 2 cut(s) 174, 1055
EaeI YGGCCR 2 cut(s) 157, 366
Eam1104I CTCTTC 1 cut(s) 1115
Eam1105I GACNNNNNGTC 2 cut(s) 174, 1055
EarI CTCTTC 1 cut(s) 1115
Eco130I CCWWGG 3 cut(s) 160, 193, 583
Eco47I GGWCC 2 cut(s) 6, 146
Eco57I CTGAAG 1 cut(s) 1060
Eco91I GGTNACC 1 cut(s) 472
EcoO65I GGTNACC 1 cut(s) 472
EcoRI GAATTC 1 cut(s) 715
EcoRII CCWGG 2 cut(s) 396, 851
EcoT14I CCWWGG 3 cut(s) 160, 193, 583
ErhI CCWWGG 3 cut(s) 160, 193, 583
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 4 cut(s) 659, 1001, 1006, 1068
FalI AAGNNNNNCTT 1 cut(s) 1127
FaqI GGGAC 2 cut(s) 26, 282
FatI CATG 4 cut(s) 655, 997, 1002, 1064
FbaI TGATCA 2 cut(s) 79, 304
FblI GTMKAC 1 cut(s) 960
Fnu4HI GCNGC 2 cut(s) 153, 933
FokI GGATG 6 cut(s) 20, 30, 380, 574, 838, 1025
Fsp4HI GCNGC 2 cut(s) 153, 933
FspBI CTAG 2 cut(s) 551, 641
GluI GCNGC 2 cut(s) 153, 933
GsaI CCCAGC 1 cut(s) 443
HaeIII GGCC 3 cut(s) 159, 368, 845
HapII CCGG 3 cut(s) 156, 412, 691
HgaI GACGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 22
Hin1II CATG 4 cut(s) 659, 1001, 1006, 1068
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 2 cut(s) 816, 906
HinfI GANTC 4 cut(s) 198, 528, 614, 1061
HpaI GTTAAC 1 cut(s) 388
HpaII CCGG 3 cut(s) 156, 412, 691
HphI GGTGA 5 cut(s) 259, 268, 466, 506, 601
Hpy166II GTNNAC 4 cut(s) 388, 796, 894, 961
Hpy188I TCNGA 7 cut(s) 146, 448, 619, 714, 1090, 1099, 1113
Hpy188III TCNNGA 5 cut(s) 299, 504, 656, 691, 720
Hpy8I GTNNAC 4 cut(s) 388, 796, 894, 961
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 2 cut(s) 556, 697
HpyCH4III ACNGT 3 cut(s) 271, 328, 929
HpyCH4V TGCA 5 cut(s) 88, 460, 935, 986, 1082
HpyF3I CTNAG 1 cut(s) 616
Hsp92I GRCGYC 1 cut(s) 22
Hsp92II CATG 4 cut(s) 659, 1001, 1006, 1068
Kpn2I TCCGGA 1 cut(s) 690
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Ksp22I TGATCA 2 cut(s) 79, 304
KspAI GTTAAC 1 cut(s) 388
Kzo9I GATC 7 cut(s) 79, 304, 448, 637, 835, 880, 1037
LmnI GCTCC 1 cut(s) 414
Lsp1109I GCAGC 2 cut(s) 139, 919
LweI GCATC 4 cut(s) 452, 507, 816, 1091
MaeI CTAG 2 cut(s) 551, 641
MaeIII GTNAC 4 cut(s) 265, 322, 472, 864
MalI GATC 7 cut(s) 81, 306, 450, 639, 837, 882, 1039
MboI GATC 7 cut(s) 79, 304, 448, 637, 835, 880, 1037
MboII GAAGA 7 cut(s) 61, 265, 598, 929, 1066, 1077, 1132
MfeI CAATTG 2 cut(s) 83, 964
MhlI GDGCHC 1 cut(s) 111
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 9 cut(s) 83, 125, 286, 350, 486, 667, 715, 964, 992
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 1 cut(s) 608
MnlI CCTC 8 cut(s) 41, 47, 54, 351, 559, 562, 720, 973
Mox20I TGGCCA 1 cut(s) 368
MroI TCCGGA 1 cut(s) 690
MroNI GCCGGC 1 cut(s) 155
MroXI GAANNNNTTC 1 cut(s) 1073
MscI TGGCCA 1 cut(s) 368
MseI TTAA 5 cut(s) 210, 387, 431, 758, 1017
MslI CAYNNNNRTG 2 cut(s) 398, 465
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 3 cut(s) 156, 412, 691
MspR9I CCNGG 3 cut(s) 398, 412, 853
MunI CAATTG 2 cut(s) 83, 964
MvaI CCWGG 2 cut(s) 398, 853
MvnI CGCG 1 cut(s) 505
NaeI GCCGGC 1 cut(s) 157
NciI CCSGG 1 cut(s) 412
NdeII GATC 7 cut(s) 79, 304, 448, 637, 835, 880, 1037
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 4 cut(s) 659, 1001, 1006, 1068
NmuCI GTSAC 3 cut(s) 265, 472, 864
NruI TCGCGA 1 cut(s) 505
NspI RCATGY 1 cut(s) 1006
PaeI GCATGC 1 cut(s) 1006
PagI TCATGA 1 cut(s) 655
PdiI GCCGGC 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 1073
PfeI GAWTC 3 cut(s) 198, 528, 1061
PkrI GCNGC 2 cut(s) 154, 934
PleI GAGTC 1 cut(s) 608
PpsI GAGTC 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 396, 851
PspEI GGTNACC 1 cut(s) 472
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 2 cut(s) 396, 851
PspPI GGNCC 3 cut(s) 6, 146, 844
PstI CTGCAG 1 cut(s) 937
PstNI CAGNNNCTG 1 cut(s) 446
RruI TCGCGA 1 cut(s) 505
RsaI GTAC 3 cut(s) 742, 786, 1139
RsaNI GTAC 3 cut(s) 741, 785, 1138
RseI CAYNNNNRTG 2 cut(s) 398, 465
SaqAI TTAA 5 cut(s) 210, 387, 431, 758, 1017
SatI GCNGC 2 cut(s) 153, 933
Sau3AI GATC 7 cut(s) 79, 304, 448, 637, 835, 880, 1037
Sau96I GGNCC 3 cut(s) 6, 146, 844
ScaI AGTACT 1 cut(s) 786
SchI GAGTC 1 cut(s) 608
ScrFI CCNGG 3 cut(s) 398, 412, 853
SduI GDGCHC 1 cut(s) 111
SfaNI GCATC 4 cut(s) 452, 507, 816, 1091
SfcI CTRYAG 1 cut(s) 933
SinI GGWCC 2 cut(s) 6, 146
SmiMI CAYNNNNRTG 2 cut(s) 398, 465
SphI GCATGC 1 cut(s) 1006
Sse9I AATT 9 cut(s) 83, 125, 286, 350, 486, 667, 715, 964, 992
SspI AATATT 2 cut(s) 379, 500
SspMI CTAG 2 cut(s) 551, 641
StyD4I CCNGG 3 cut(s) 396, 410, 851
StyI CCWWGG 3 cut(s) 160, 193, 583
TaaI ACNGT 3 cut(s) 271, 328, 929
TaqI TCGA 1 cut(s) 1059
TasI AATT 9 cut(s) 83, 125, 286, 350, 486, 667, 715, 964, 992
TatI WGTACW 1 cut(s) 784
TfiI GAWTC 3 cut(s) 198, 528, 1061
Tru1I TTAA 5 cut(s) 210, 387, 431, 758, 1017
Tru9I TTAA 5 cut(s) 210, 387, 431, 758, 1017
TscAI CASTG 5 cut(s) 235, 241, 687, 942, 1084
TseFI GTSAC 3 cut(s) 265, 472, 864
TseI GCWGC 2 cut(s) 152, 932
Tsp45I GTSAC 3 cut(s) 265, 472, 864
TspDTI ATGAA 8 cut(s) 382, 584, 644, 662, 827, 901, 986, 1016
TspGWI ACGGA 1 cut(s) 838
TspRI CASTG 5 cut(s) 235, 241, 687, 942, 1084
VpaK11BI GGWCC 2 cut(s) 6, 146
XapI RAATTY 3 cut(s) 125, 667, 715
XceI RCATGY 1 cut(s) 1006
XmiI GTMKAC 1 cut(s) 960
XmnI GAANNNNTTC 1 cut(s) 1073
XspI CTAG 2 cut(s) 551, 641
ZrmI AGTACT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.