pycom5153684g00430

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012527_4311101_to_5153684
OG0015348
Physical Location & Seq
Forward (+)
742523 .. 744832
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom5153684g00430.3

Sequence Viewer

Length: 1182 bp
ATGAGAGTGTGCAGCGGGTGGCGCCGTTTCTTCATCTTTCTACCTCTTATTTTCATTCTTCGTCACTTCTTCTCCGTTAAGGAATTGCATCATAACTCTGCTGTGGAAGATTTGCCCAGGAAGAAAGAAAGCAACAAATCCAAATCCAACCACTTGGTTCTTGGCCCTGCTGCTGGCCAAGGCTTGAGCGATCGCCTCCAATGTCATGGATCTGGCAATGGCATCAAAGCTCTCAACCACACCACCCATTCTTCCCGTGCCTCCGCTGCGGTTGATAGCATTGCTGTTGTCACTGTCTTCACCATATACAATTCTTCCACCTCCAATAGGCACGTCGAAGATCAATTCTCAAACTCTGTTACAGTTGGGAATGCTTCTTATACTAAATTCGAGAGATCCATGGCCGTCTTGAATGTCTTTATTAACTTCATTCAGCTGGCTATGCCCCGGAGCAGTGTAGTCATTCTAACAGACCCGGCAGCTGATCTTTCAATACGCAGAAACAAGGTCACTGTATATCCAATTCAAGGTGAATATTCACGAGACAAGTTGATGCTTCAGAGAATCAGGTCTTACATTGCCTTCCTAGAATCTAGGATGGAGGAGCTTCCTCAGAATCAGAAGCATACTCATTACATCTTCACCGACTCAGATATCACTGTTGTTGATGATCTAGGACACATATTTAGTGAATATCCGAATTTTCATCTGGCACTTACGTTTCGCAATAACAAAGCACAACCTCTGAATTCAGGATTTATAGCAGTGAGGGGCACCGCTGATGGAATTTTAAGGGCAAAGATTTTTCTACAAAAAGTACTAAAAGTTTATCAAACAAGGTACATGAGGGCTTCTAGAATGCTTGGGGATCAATTAGCTCTTGCCTGGGTAGTGACGTCGCACCCATCTTTCGATGCAAAGAAATTTACAAAAGCCAAAGCTTTCGTAGATGATATTCACGGTGCTTCCGTGTTGTTTTTACCCTGTGCTGTGTACAACTGGACACCACCAGAAGGCGCTGGCCAATTTCATGGCATGCCCTTGGATGTAAAGGTTGTTCATTTCAAAGGATCAAGGAAACGCCTGATGCTCGAGTCTTGGAACTTCTACAGTTCCGTTTCCAACATTCCAGATATGTTGTGCCTGATATTAAAGAGTGGGAGAACAAAGTATGACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

44.46

Weight (kDa)

9.74

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45320
fragaria_vesca FvH4_1g28100 FvH4_1g28100
malus_domestica MD00G1094900.v1.1
prunus_persica Prupe.6G155400_v2.0.a1
pyrus_communis pycom5153684g00430
rosa_chinensis RchiOBHm_Chr3g0497141
rosa_laevigata RLG00000022478
rosa_multiflora Rmu_sc0002893.1_g000029
rosa_roxburghii Rroxscaffold_6G00388380
rosa_rugosa Rorug03G0291300
rosa_samantha Rh3AG334600 Rh3BG371700 Rh3CG368400 Rh3DG369400
rosa_wichuraiana Rw3G029360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 899
AccB1I GGYRCC 2 cut(s) 21, 773
AciI CCGC 4 cut(s) 15, 264, 269, 777
AclWI GGATC 4 cut(s) 217, 390, 876, 1078
AcoI YGGCCR 3 cut(s) 175, 402, 1021
AcsI RAATTY 5 cut(s) 386, 700, 748, 786, 923
AcuI CTGAAG 1 cut(s) 542
AcyI GRCGYC 2 cut(s) 22, 896
AfaI GTAC 3 cut(s) 819, 842, 995
AfiI CCNNNNNNNGG 4 cut(s) 173, 327, 527, 1013
AgsI TTSAA 4 cut(s) 412, 492, 527, 1066
AjiI CACGTC 1 cut(s) 334
AjnI CCWGG 2 cut(s) 116, 884
AluBI AGCT 6 cut(s) 230, 436, 482, 607, 878, 941
AluI AGCT 6 cut(s) 230, 436, 482, 607, 878, 941
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 4 cut(s) 217, 390, 876, 1078
Ama87I CYCGRG 1 cut(s) 1091
AoxI GGCC 4 cut(s) 163, 175, 402, 1021
ApeKI GCWGC 4 cut(s) 12, 170, 266, 479
ApoI RAATTY 5 cut(s) 386, 700, 748, 786, 923
AsiSI GCGATCGC 1 cut(s) 193
AspLEI GCGC 2 cut(s) 24, 1019
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 2 cut(s) 448, 476
AsuHPI GGTGA 3 cut(s) 292, 542, 634
AvaI CYCGRG 1 cut(s) 1091
BaeGI GKGCMC 1 cut(s) 776
BalI TGGCCA 2 cut(s) 177, 1023
BanI GGYRCC 2 cut(s) 21, 773
BauI CACGAG 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 289
BbvI GCAGC 4 cut(s) 24, 157, 253, 491
BccI CCATC 3 cut(s) 592, 776, 913
BceAI ACGGC 2 cut(s) 9, 389
BciT130I CCWGG 2 cut(s) 118, 886
BcnI CCSGG 2 cut(s) 448, 476
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 4 cut(s) 587, 594, 674, 855
BfmI CTRYAG 1 cut(s) 1108
BfoI RGCGCY 2 cut(s) 25, 1020
BisI GCNGC 4 cut(s) 13, 171, 267, 480
BlsI GCNGC 4 cut(s) 14, 172, 268, 481
BmcAI AGTACT 1 cut(s) 819
Bme1390I CCNGG 4 cut(s) 118, 448, 476, 886
BmeT110I CYCGRG 1 cut(s) 1091
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 23, 775
BmrFI CCNGG 4 cut(s) 118, 448, 476, 886
BmsI GCATC 5 cut(s) 97, 231, 543, 904, 1077
BpiI GAAGAC 1 cut(s) 289
BpuEI CTTGAG 1 cut(s) 205
BpuMI CCSGG 2 cut(s) 448, 476
BsaHI GRCGYC 2 cut(s) 22, 896
BsaJI CCNNGG 6 cut(s) 116, 178, 399, 446, 885, 1041
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 327, 527, 1013
Bse1I ACTGG 1 cut(s) 1004
Bse3DI GCAATG 3 cut(s) 223, 279, 576
BseBI CCWGG 2 cut(s) 118, 886
BseDI CCNNGG 6 cut(s) 116, 178, 399, 446, 885, 1041
BseGI GGATG 2 cut(s) 603, 1051
BseLI CCNNNNNNNGG 4 cut(s) 173, 327, 527, 1013
BseMI GCAATG 3 cut(s) 223, 279, 576
BseMII CTCAG 2 cut(s) 626, 663
BseNI ACTGG 1 cut(s) 1004
BseRI GAGGAG 1 cut(s) 617
BseSI GKGCMC 1 cut(s) 776
BseXI GCAGC 4 cut(s) 24, 157, 253, 491
BsgI GTGCAG 1 cut(s) 31
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 4 cut(s) 165, 177, 404, 1023
BshNI GGYRCC 2 cut(s) 21, 773
BsiEI CGRYCG 1 cut(s) 193
BsiHKCI CYCGRG 1 cut(s) 1091
BsiSI CCGG 2 cut(s) 448, 476
BslI CCNNNNNNNGG 4 cut(s) 173, 327, 527, 1013
BsmAI GTCTC 1 cut(s) 537
BsmI GAATGC 2 cut(s) 376, 864
BsnI GGCC 4 cut(s) 165, 177, 404, 1023
BsoBI CYCGRG 1 cut(s) 1091
Bsp1286I GDGCHC 1 cut(s) 776
Bsp1407I TGTACA 1 cut(s) 993
Bsp143I GATC 8 cut(s) 190, 209, 340, 395, 484, 670, 868, 1070
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 4 cut(s) 15, 264, 269, 777
BspANI GGCC 4 cut(s) 165, 177, 404, 1023
BspCNI CTCAG 2 cut(s) 625, 662
BspLI GGNNCC 2 cut(s) 23, 775
BspPI GGATC 4 cut(s) 217, 390, 876, 1078
BspT107I GGYRCC 2 cut(s) 21, 773
BsrDI GCAATG 3 cut(s) 223, 279, 576
BsrGI TGTACA 1 cut(s) 993
BsrI ACTGG 1 cut(s) 1004
BssECI CCNNGG 6 cut(s) 116, 178, 399, 446, 885, 1041
BssMI GATC 8 cut(s) 190, 209, 340, 395, 484, 670, 868, 1070
BssNI GRCGYC 2 cut(s) 22, 896
BssSI CACGAG 1 cut(s) 540
BssT1I CCWWGG 3 cut(s) 178, 399, 1041
Bst2BI CACGAG 1 cut(s) 540
Bst2UI CCWGG 2 cut(s) 118, 886
Bst4CI ACNGT 6 cut(s) 295, 364, 514, 661, 962, 1112
BstACI GRCGYC 2 cut(s) 22, 896
BstAUI TGTACA 1 cut(s) 993
BstC8I GCNNGC 4 cut(s) 175, 438, 1021, 1037
BstDEI CTNAG 2 cut(s) 612, 649
BstDSI CCRYGG 1 cut(s) 399
BstENI CCTNNNNNAGG 1 cut(s) 325
BstF5I GGATG 2 cut(s) 603, 1051
BstH2I RGCGCY 2 cut(s) 25, 1020
BstHHI GCGC 2 cut(s) 24, 1019
BstKTI GATC 8 cut(s) 193, 212, 343, 398, 487, 673, 871, 1073
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 8 cut(s) 190, 209, 340, 395, 484, 670, 868, 1070
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 3 cut(s) 21, 266, 442
BstNI CCWGG 2 cut(s) 118, 886
BstNSI RCATGY 1 cut(s) 1039
BstSCI CCNGG 4 cut(s) 116, 446, 474, 884
BstSFI CTRYAG 1 cut(s) 1108
BstSLI GKGCMC 1 cut(s) 776
BstV1I GCAGC 4 cut(s) 24, 157, 253, 491
BstV2I GAAGAC 1 cut(s) 289
BstX2I RGATCY 2 cut(s) 209, 395
BstXI CCANNNNNNTGG 3 cut(s) 154, 206, 1031
BstYI RGATCY 2 cut(s) 209, 395
BsuRI GGCC 4 cut(s) 165, 177, 404, 1023
BtgI CCRYGG 1 cut(s) 399
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 2 cut(s) 603, 1051
BtsI GCAGTG 2 cut(s) 460, 771
BtsIMutI CAGTG 5 cut(s) 291, 460, 510, 657, 771
Cac8I GCNNGC 4 cut(s) 175, 438, 1021, 1037
CfoI GCGC 2 cut(s) 24, 1019
Cfr13I GGNCC 1 cut(s) 164
Csp6I GTAC 3 cut(s) 818, 841, 994
CviAII CATG 5 cut(s) 206, 400, 844, 1031, 1036
CviQI GTAC 3 cut(s) 818, 841, 994
DdeI CTNAG 2 cut(s) 612, 649
DinI GGCGCC 1 cut(s) 23
DpnI GATC 8 cut(s) 192, 211, 342, 397, 486, 672, 870, 1072
DpnII GATC 8 cut(s) 190, 209, 340, 395, 484, 670, 868, 1070
EaeI YGGCCR 3 cut(s) 175, 402, 1021
Eco130I CCWWGG 3 cut(s) 178, 399, 1041
Eco32I GATATC 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 542
Eco88I CYCGRG 1 cut(s) 1091
EcoNI CCTNNNNNAGG 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 748
EcoRII CCWGG 2 cut(s) 116, 884
EcoRV GATATC 1 cut(s) 655
EcoT14I CCWWGG 3 cut(s) 178, 399, 1041
EgeI GGCGCC 1 cut(s) 23
EheI GGCGCC 1 cut(s) 23
ErhI CCWWGG 3 cut(s) 178, 399, 1041
FaeI CATG 5 cut(s) 209, 403, 847, 1034, 1039
FalI AAGNNNNNCTT 1 cut(s) 1160
FatI CATG 5 cut(s) 205, 399, 843, 1030, 1035
FauI CCCGC 1 cut(s) 8
Fnu4HI GCNGC 4 cut(s) 13, 171, 267, 480
FokI GGATG 2 cut(s) 610, 1058
Fsp4HI GCNGC 4 cut(s) 13, 171, 267, 480
FspBI CTAG 4 cut(s) 587, 594, 674, 855
GlaI GCGC 2 cut(s) 23, 1018
GluI GCNGC 4 cut(s) 13, 171, 267, 480
HaeII RGCGCY 2 cut(s) 25, 1020
HaeIII GGCC 4 cut(s) 165, 177, 404, 1023
HapII CCGG 2 cut(s) 448, 476
HhaI GCGC 2 cut(s) 24, 1019
Hin1I GRCGYC 2 cut(s) 22, 896
Hin1II CATG 5 cut(s) 209, 403, 847, 1034, 1039
Hin6I GCGC 2 cut(s) 22, 1017
HinP1I GCGC 2 cut(s) 22, 1017
HindIII AAGCTT 1 cut(s) 939
HinfI GANTC 5 cut(s) 564, 590, 616, 647, 1094
HpaII CCGG 2 cut(s) 448, 476
HphI GGTGA 3 cut(s) 292, 542, 634
Hpy166II GTNNAC 1 cut(s) 994
Hpy188I TCNGA 6 cut(s) 561, 615, 621, 652, 699, 747
Hpy188III TCNNGA 6 cut(s) 391, 409, 540, 753, 855, 1130
Hpy8I GTNNAC 1 cut(s) 994
Hpy99I CGWCG 2 cut(s) 338, 901
HpyAV CCTTC 2 cut(s) 592, 1007
HpyCH4III ACNGT 6 cut(s) 295, 364, 514, 661, 962, 1112
HpyCH4IV ACGT 3 cut(s) 333, 719, 896
HpyCH4V TGCA 3 cut(s) 12, 88, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 266, 442
HpyF3I CTNAG 2 cut(s) 612, 649
HpySE526I ACGT 3 cut(s) 333, 719, 896
Hsp92I GRCGYC 2 cut(s) 22, 896
Hsp92II CATG 5 cut(s) 209, 403, 847, 1034, 1039
HspAI GCGC 2 cut(s) 22, 1017
KasI GGCGCC 1 cut(s) 21
Kzo9I GATC 8 cut(s) 190, 209, 340, 395, 484, 670, 868, 1070
LmnI GCTCC 2 cut(s) 450, 604
Lsp1109I GCAGC 4 cut(s) 24, 157, 253, 491
LweI GCATC 5 cut(s) 97, 231, 543, 904, 1077
MaeI CTAG 4 cut(s) 587, 594, 674, 855
MaeII ACGT 3 cut(s) 333, 719, 896
MaeIII GTNAC 5 cut(s) 62, 289, 358, 508, 892
MalI GATC 8 cut(s) 192, 211, 342, 397, 486, 672, 870, 1072
MboI GATC 8 cut(s) 190, 209, 340, 395, 484, 670, 868, 1070
MflI RGATCY 2 cut(s) 209, 395
MhlI GDGCHC 1 cut(s) 776
MlsI TGGCCA 2 cut(s) 177, 1023
MluNI TGGCCA 2 cut(s) 177, 1023
Mly113I GGCGCC 1 cut(s) 22
MlyI GAGTC 2 cut(s) 641, 1103
MmeI TCCRAC 2 cut(s) 171, 1146
MnlI CCTC 9 cut(s) 54, 206, 271, 331, 595, 621, 753, 762, 840
Mox20I TGGCCA 2 cut(s) 177, 1023
MscI TGGCCA 2 cut(s) 177, 1023
MseI TTAA 4 cut(s) 78, 423, 791, 1151
Msp20I TGGCCA 2 cut(s) 177, 1023
MspA1I CMGCKG 5 cut(s) 15, 266, 436, 482, 779
MspI CCGG 2 cut(s) 448, 476
MspR9I CCNGG 4 cut(s) 118, 448, 476, 886
Mva1269I GAATGC 2 cut(s) 376, 864
MvaI CCWGG 2 cut(s) 118, 886
MwoI GCNNNNNNNGC 3 cut(s) 21, 266, 442
NarI GGCGCC 1 cut(s) 22
NciI CCSGG 2 cut(s) 448, 476
NcoI CCATGG 1 cut(s) 399
NdeII GATC 8 cut(s) 190, 209, 340, 395, 484, 670, 868, 1070
NlaIII CATG 5 cut(s) 209, 403, 847, 1034, 1039
NlaIV GGNNCC 2 cut(s) 23, 775
NmuCI GTSAC 4 cut(s) 62, 289, 508, 892
NspI RCATGY 1 cut(s) 1039
PaeI GCATGC 1 cut(s) 1039
PaeR7I CTCGAG 1 cut(s) 1091
PcsI WCGNNNNNNNCGW 1 cut(s) 966
PctI GAATGC 2 cut(s) 376, 864
PfeI GAWTC 3 cut(s) 564, 590, 616
PkrI GCNGC 4 cut(s) 14, 172, 268, 481
Ple19I CGATCG 1 cut(s) 193
PleI GAGTC 2 cut(s) 641, 1102
PluTI GGCGCC 1 cut(s) 25
PpsI GAGTC 2 cut(s) 641, 1102
Psp6I CCWGG 2 cut(s) 116, 884
PspGI CCWGG 2 cut(s) 116, 884
PspN4I GGNNCC 2 cut(s) 23, 775
PspPI GGNCC 1 cut(s) 164
PspXI VCTCGAGB 1 cut(s) 1091
PsrI GAACNNNNNNTAC 2 cut(s) 1041, 1073
PsuI RGATCY 2 cut(s) 209, 395
PvuI CGATCG 1 cut(s) 193
PvuII CAGCTG 2 cut(s) 436, 482
RgaI GCGATCGC 1 cut(s) 193
RsaI GTAC 3 cut(s) 819, 842, 995
RsaNI GTAC 3 cut(s) 818, 841, 994
SaqAI TTAA 4 cut(s) 78, 423, 791, 1151
SatI GCNGC 4 cut(s) 13, 171, 267, 480
Sau3AI GATC 8 cut(s) 190, 209, 340, 395, 484, 670, 868, 1070
Sau96I GGNCC 1 cut(s) 164
ScaI AGTACT 1 cut(s) 819
SchI GAGTC 2 cut(s) 641, 1103
ScrFI CCNGG 4 cut(s) 118, 448, 476, 886
SduI GDGCHC 1 cut(s) 776
SfaAI GCGATCGC 1 cut(s) 193
SfaNI GCATC 5 cut(s) 97, 231, 543, 904, 1077
SfcI CTRYAG 1 cut(s) 1108
SfoI GGCGCC 1 cut(s) 23
Sfr274I CTCGAG 1 cut(s) 1091
SgfI GCGATCGC 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 1091
SmlI CTYRAG 2 cut(s) 184, 1091
SmoI CTYRAG 2 cut(s) 184, 1091
SphI GCATGC 1 cut(s) 1039
SsiI CCGC 4 cut(s) 15, 264, 269, 777
SspDI GGCGCC 1 cut(s) 21
SspI AATATT 1 cut(s) 536
SspMI CTAG 4 cut(s) 587, 594, 674, 855
StyD4I CCNGG 4 cut(s) 116, 446, 474, 884
StyI CCWWGG 3 cut(s) 178, 399, 1041
TaaI ACNGT 6 cut(s) 295, 364, 514, 661, 962, 1112
TaiI ACGT 3 cut(s) 336, 722, 899
TaqI TCGA 4 cut(s) 336, 390, 912, 1092
TatI WGTACW 2 cut(s) 817, 993
TfiI GAWTC 3 cut(s) 564, 590, 616
Tru1I TTAA 4 cut(s) 78, 423, 791, 1151
Tru9I TTAA 4 cut(s) 78, 423, 791, 1151
TscAI CASTG 5 cut(s) 298, 460, 517, 664, 771
TseFI GTSAC 4 cut(s) 62, 289, 508, 892
TseI GCWGC 4 cut(s) 12, 170, 266, 479
Tsp45I GTSAC 4 cut(s) 62, 289, 508, 892
TspDTI ATGAA 6 cut(s) 22, 43, 418, 695, 1019, 1049
TspGWI ACGGA 3 cut(s) 64, 958, 1105
TspRI CASTG 5 cut(s) 298, 460, 517, 664, 771
XagI CCTNNNNNAGG 1 cut(s) 325
XapI RAATTY 5 cut(s) 386, 700, 748, 786, 923
XbaI TCTAGA 1 cut(s) 854
XceI RCATGY 1 cut(s) 1039
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XhoI CTCGAG 1 cut(s) 1091
XspI CTAG 4 cut(s) 587, 594, 674, 855
ZraI GACGTC 1 cut(s) 897
ZrmI AGTACT 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.