MD00G1122900.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015223
Physical Location & Seq
Reverse (-)
26375883 .. 26377457
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1122900.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGGACCTCTCTTTCCTCTGCCTCCTCCTACTCTTCTCAACCGCTGCATTTGTCAATGTTGATGCCTCTTTTACCGGAACATATGGAGTAAACTATGGCAGGATCGCCGACAATATACCTTCGCCTCACAGCGTGGTGACGCTTCTTAAAGCGGCAAAGATAAAAAACATCAGGATATATGACGCTGATCATGAAGTTCTCACCGCCTTCAAGGGATCAGGTATAGAAATAATTGTAGGACTGGGAAATGAGTTCCTCAAAGACATCAGTGTGAACGAGGATCGCGCCACAAATTGGATAAAAGAAAATGTGCAGCCATACATTCCGGGGACTCACATTTCAGGAATAGCAGTGGGGAATGAGATCTTGGGGGGAACTGATATAGAGCTCTCAGAGGTCTTATTGCCTGCCGTAAAGAACGTTTACAATGCGCTTAGCCGGCTTGATTTGGCTAACTCCATTCAGGTCTCGAGTCCACATTCGGAGGCTGTGTTTGCAAATTCCTACCCACCATCGGCCTGCATTTTCAAGGAAGATGTTGCTCAATTCATGAAGCCGCTTTTGGAATTTTTCTCACAGATCAAATCCCCATTTTATATAAATGCATATCCATTTCTGGCATATAAGAGTGACCCTGAGCACATTGACCTTAACTATGCTCTCTTCAAGAAAAATGCCGGGATTCGTGATACAAAAACCAATCTACATTATGACAACATGTTTGATGCAATGGTTGATGCGTCGTATTTTGCTCTGGAGAAGGCTGGATTTGACAAGACAGAGGTCATAGTTTCCGAAACTGGTTGGGCGTCTCGCGGGGATGAAAGTGAAGCAGGAGCCAACGCCAAAAATGCCAGGACTTACAACTACAACCTGCGGAAACGGCTGACGAAGAAGAAGGGGACGCCTCATAGGCCAAAGACGGTTGTGAGAGCTTACATTTTTGCTTTGTTCAACGAGAATTTGAAACCGGGGCCGACTTCCGAGAGGAATTTCGGATTGTTCAAAGCTGATGGGAGCATTTCATACGACATTGGCTTCACTGGACTTGTTGGACCTTCTGCAGCATCCTCATCTCTTCGTAACTACAAAGTTTTCACAAGTTGTGCAGCTCTTCTGCTACTATTGTTAGCATCATAA

Protein Analysis

380

Amino Acids

41.65

Weight (kDa)

6.67

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 29 - 347 4.6e-86 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15800
fragaria_vesca FvH4_6g29810
malus_domestica MD00G1122900.v1.1 MD17G1210800.v1.1
prunus_persica Prupe.3G086200_v2.0.a1
pyrus_communis pycom09g14110 pycom17g21580
rosa_chinensis RchiOBHm_Chr2g0135631
rosa_laevigata RLG00000019473
rosa_multiflora Rmu_sc0002398.1_g000035
rosa_roxburghii Rroxscaffold_2G00109030
rosa_rugosa Rorug02G0325300
rosa_samantha Rh2AG376700 Rh2BG383000 Rh2DG399100
rosa_wichuraiana Rw2G030720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 882
AccB7I CCANNNNNTGG 1 cut(s) 294
AccII CGCG 2 cut(s) 285, 816
AciI CCGC 6 cut(s) 42, 152, 204, 557, 816, 877
AclI AACGTT 1 cut(s) 420
AclWI GGATC 3 cut(s) 110, 223, 288
AcsI RAATTY 4 cut(s) 499, 566, 961, 991
AcyI GRCGYC 2 cut(s) 809, 905
AdeI CACNNNGTG 1 cut(s) 133
AfiI CCNNNNNNNGG 2 cut(s) 294, 514
AflIII ACRYGT 1 cut(s) 717
AgsI TTSAA 6 cut(s) 211, 529, 667, 955, 967, 1006
AjnI CCWGG 1 cut(s) 854
AjuI GAANNNNNNNTTGG 4 cut(s) 350, 382, 545, 577
AluBI AGCT 4 cut(s) 388, 935, 1010, 1112
AluI AGCT 4 cut(s) 388, 935, 1010, 1112
Alw21I GWGCWC 2 cut(s) 390, 642
Alw26I GTCTC 2 cut(s) 472, 816
AlwI GGATC 3 cut(s) 110, 223, 288
Ama87I CYCGRG 1 cut(s) 469
AoxI GGCC 3 cut(s) 516, 914, 974
ApeKI GCWGC 4 cut(s) 44, 313, 1064, 1109
ApoI RAATTY 4 cut(s) 499, 566, 961, 991
ArsI GACNNNNNNTTYG 2 cut(s) 704, 736
AspLEI GCGC 2 cut(s) 287, 433
AspS9I GGNCC 3 cut(s) 4, 974, 1055
AsuC2I CCSGG 3 cut(s) 327, 679, 972
AsuHPI GGTGA 2 cut(s) 148, 193
AvaI CYCGRG 1 cut(s) 469
AvaII GGWCC 2 cut(s) 4, 1055
BanII GRGCYC 1 cut(s) 390
Bbv12I GWGCWC 2 cut(s) 390, 642
BbvI GCAGC 4 cut(s) 31, 325, 1076, 1121
BccI CCATC 2 cut(s) 520, 1007
BceAI ACGGC 2 cut(s) 395, 899
BciT130I CCWGG 1 cut(s) 856
BclI TGATCA 1 cut(s) 187
BcnI CCSGG 3 cut(s) 327, 679, 972
BcoDI GTCTC 2 cut(s) 472, 816
BfmI CTRYAG 1 cut(s) 1062
BfuAI ACCTGC 1 cut(s) 882
BglI GCCNNNNNGGC 1 cut(s) 913
BglII AGATCT 1 cut(s) 363
BisI GCNGC 6 cut(s) 45, 153, 314, 557, 1065, 1110
BlpI GCTNAGC 1 cut(s) 434
BlsI GCNGC 6 cut(s) 46, 154, 315, 558, 1066, 1111
Bme1390I CCNGG 4 cut(s) 327, 679, 856, 972
Bme18I GGWCC 2 cut(s) 4, 1055
BmeT110I CYCGRG 1 cut(s) 469
BmgT120I GGNCC 3 cut(s) 4, 974, 1055
BmiI GGNNCC 2 cut(s) 838, 975
BmrFI CCNGG 4 cut(s) 327, 679, 856, 972
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 4 cut(s) 52, 715, 727, 1076
BmuI ACTGGG 1 cut(s) 251
BoxI GACNNNNGTC 1 cut(s) 782
BpmI CTGGAG 1 cut(s) 776
Bpu10I CCTNAGC 1 cut(s) 636
Bpu1102I GCTNAGC 1 cut(s) 434
BpuMI CCSGG 3 cut(s) 327, 679, 972
BsaHI GRCGYC 2 cut(s) 809, 905
BsaI GGTCTC 1 cut(s) 472
BsaJI CCNNGG 2 cut(s) 326, 971
BsaWI WCCGGW 1 cut(s) 74
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 514
Bse118I RCCGGY 1 cut(s) 438
Bse1I ACTGG 3 cut(s) 246, 805, 1048
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 1 cut(s) 856
BseDI CCNNGG 2 cut(s) 326, 971
BseGI GGATG 2 cut(s) 826, 1067
BseLI CCNNNNNNNGG 2 cut(s) 294, 514
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 2 cut(s) 405, 627
BseNI ACTGG 3 cut(s) 246, 805, 1048
BseRI GAGGAG 1 cut(s) 14
BseXI GCAGC 4 cut(s) 31, 325, 1076, 1121
BsgI GTGCAG 2 cut(s) 332, 1128
Bsh1236I CGCG 2 cut(s) 285, 816
BshFI GGCC 3 cut(s) 518, 916, 976
BsiHKAI GWGCWC 2 cut(s) 390, 642
BsiHKCI CYCGRG 1 cut(s) 469
BsiSI CCGG 5 cut(s) 75, 326, 439, 678, 971
BslFI GGGAC 2 cut(s) 343, 916
BslI CCNNNNNNNGG 2 cut(s) 294, 514
BsmAI GTCTC 2 cut(s) 472, 816
BsmBI CGTCTC 1 cut(s) 816
BsmFI GGGAC 2 cut(s) 343, 916
BsnI GGCC 3 cut(s) 518, 916, 976
Bso31I GGTCTC 1 cut(s) 472
BsoBI CYCGRG 1 cut(s) 469
Bsp1286I GDGCHC 2 cut(s) 390, 642
Bsp143I GATC 6 cut(s) 102, 187, 215, 280, 363, 579
Bsp1720I GCTNAGC 1 cut(s) 434
BspACI CCGC 6 cut(s) 42, 152, 204, 557, 816, 877
BspANI GGCC 3 cut(s) 518, 916, 976
BspCNI CTCAG 2 cut(s) 404, 628
BspFNI CGCG 2 cut(s) 285, 816
BspHI TCATGA 2 cut(s) 190, 549
BspLI GGNNCC 2 cut(s) 838, 975
BspMAI CTGCAG 1 cut(s) 1066
BspMI ACCTGC 1 cut(s) 882
BspPI GGATC 3 cut(s) 110, 223, 288
BspQI GCTCTTC 1 cut(s) 1119
BspTNI GGTCTC 1 cut(s) 472
BsrDI GCAATG 1 cut(s) 735
BsrFI RCCGGY 1 cut(s) 438
BsrI ACTGG 3 cut(s) 246, 805, 1048
BssAI RCCGGY 1 cut(s) 438
BssECI CCNNGG 2 cut(s) 326, 971
BssMI GATC 6 cut(s) 102, 187, 215, 280, 363, 579
BssNI GRCGYC 2 cut(s) 809, 905
Bst2UI CCWGG 1 cut(s) 856
Bst4CI ACNGT 1 cut(s) 925
Bst6I CTCTTC 4 cut(s) 38, 668, 1083, 1119
BstACI GRCGYC 2 cut(s) 809, 905
BstC8I GCNNGC 3 cut(s) 408, 440, 520
BstDEI CTNAG 3 cut(s) 391, 434, 636
BstF5I GGATG 2 cut(s) 826, 1067
BstFNI CGCG 2 cut(s) 285, 816
BstHHI GCGC 2 cut(s) 287, 433
BstKTI GATC 6 cut(s) 105, 190, 218, 283, 366, 582
BstMAI GTCTC 2 cut(s) 472, 816
BstMBI GATC 6 cut(s) 102, 187, 215, 280, 363, 579
BstMWI GCNNNNNNNGC 5 cut(s) 439, 494, 851, 883, 913
BstNI CCWGG 1 cut(s) 856
BstNSI RCATGY 1 cut(s) 721
BstPAI GACNNNNGTC 1 cut(s) 782
BstSCI CCNGG 4 cut(s) 325, 677, 854, 970
BstSFI CTRYAG 1 cut(s) 1062
BstUI CGCG 2 cut(s) 285, 816
BstV1I GCAGC 4 cut(s) 31, 325, 1076, 1121
BstX2I RGATCY 1 cut(s) 363
BstYI RGATCY 1 cut(s) 363
BsuRI GGCC 3 cut(s) 518, 916, 976
BtsCI GGATG 2 cut(s) 826, 1067
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 3 cut(s) 274, 357, 1041
BveI ACCTGC 1 cut(s) 882
Cac8I GCNNGC 3 cut(s) 408, 440, 520
CciI TCATGA 2 cut(s) 190, 549
CfoI GCGC 2 cut(s) 287, 433
Cfr10I RCCGGY 1 cut(s) 438
Cfr13I GGNCC 3 cut(s) 4, 974, 1055
CseI GACGC 5 cut(s) 148, 191, 729, 798, 913
CviAII CATG 3 cut(s) 191, 550, 718
DdeI CTNAG 3 cut(s) 391, 434, 636
DpnI GATC 6 cut(s) 104, 189, 217, 282, 365, 581
DpnII GATC 6 cut(s) 102, 187, 215, 280, 363, 579
DraIII CACNNNGTG 1 cut(s) 133
Eam1104I CTCTTC 4 cut(s) 38, 668, 1083, 1119
EarI CTCTTC 4 cut(s) 38, 668, 1083, 1119
Ecl136II GAGCTC 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 472
Eco47I GGWCC 2 cut(s) 4, 1055
Eco53kI GAGCTC 1 cut(s) 388
Eco88I CYCGRG 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 854
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 390
Esp3I CGTCTC 1 cut(s) 816
FaeI CATG 3 cut(s) 194, 553, 721
FaqI GGGAC 2 cut(s) 343, 916
FatI CATG 3 cut(s) 190, 549, 717
FauI CCCGC 1 cut(s) 809
FauNDI CATATG 1 cut(s) 82
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 6 cut(s) 45, 153, 314, 557, 1065, 1110
FokI GGATG 2 cut(s) 833, 1054
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 6 cut(s) 45, 153, 314, 557, 1065, 1110
GlaI GCGC 2 cut(s) 286, 432
GluI GCNGC 6 cut(s) 45, 153, 314, 557, 1065, 1110
GsuI CTGGAG 1 cut(s) 776
HaeIII GGCC 3 cut(s) 518, 916, 976
HapII CCGG 5 cut(s) 75, 326, 439, 678, 971
HgaI GACGC 5 cut(s) 148, 191, 729, 798, 913
HhaI GCGC 2 cut(s) 287, 433
Hin1I GRCGYC 2 cut(s) 809, 905
Hin1II CATG 3 cut(s) 194, 553, 721
Hin6I GCGC 2 cut(s) 285, 431
HinP1I GCGC 2 cut(s) 285, 431
HinfI GANTC 3 cut(s) 331, 472, 682
HpaII CCGG 5 cut(s) 75, 326, 439, 678, 971
HphI GGTGA 2 cut(s) 148, 193
Hpy166II GTNNAC 4 cut(s) 91, 274, 424, 476
Hpy188I TCNGA 5 cut(s) 394, 484, 796, 985, 998
Hpy188III TCNNGA 8 cut(s) 172, 191, 342, 469, 550, 667, 686, 755
Hpy8I GTNNAC 4 cut(s) 91, 274, 424, 476
Hpy99I CGWCG 1 cut(s) 745
HpyAV CCTTC 5 cut(s) 129, 217, 754, 892, 1068
HpyCH4III ACNGT 1 cut(s) 925
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 8 cut(s) 47, 313, 497, 522, 605, 728, 1064, 1109
HpyF10VI GCNNNNNNNGC 5 cut(s) 439, 494, 851, 883, 913
HpyF3I CTNAG 3 cut(s) 391, 434, 636
HpySE526I ACGT 1 cut(s) 420
Hsp92I GRCGYC 2 cut(s) 809, 905
Hsp92II CATG 3 cut(s) 194, 553, 721
HspAI GCGC 2 cut(s) 285, 431
KroI GCCGGC 1 cut(s) 438
KroNI GCCGGC 1 cut(s) 440
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 6 cut(s) 102, 187, 215, 280, 363, 579
LguI GCTCTTC 1 cut(s) 1119
LmnI GCTCC 2 cut(s) 836, 1017
Lsp1109I GCAGC 4 cut(s) 31, 325, 1076, 1121
LweI GCATC 4 cut(s) 52, 715, 727, 1076
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 3 cut(s) 136, 629, 1082
MalI GATC 6 cut(s) 104, 189, 217, 282, 365, 581
MboI GATC 6 cut(s) 102, 187, 215, 280, 363, 579
MboII GAAGA 7 cut(s) 25, 545, 655, 904, 907, 1070, 1106
MflI RGATCY 1 cut(s) 363
MhlI GDGCHC 2 cut(s) 390, 642
MluCI AATT 7 cut(s) 231, 292, 499, 545, 566, 961, 991
MlyI GAGTC 2 cut(s) 325, 481
MmeI TCCRAC 1 cut(s) 1033
Mph1103I ATGCAT 1 cut(s) 607
MroNI GCCGGC 1 cut(s) 438
MseI TTAA 2 cut(s) 147, 651
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 5 cut(s) 75, 326, 439, 678, 971
MspR9I CCNGG 4 cut(s) 327, 679, 856, 972
MvaI CCWGG 1 cut(s) 856
MvnI CGCG 2 cut(s) 285, 816
MwoI GCNNNNNNNGC 5 cut(s) 439, 494, 851, 883, 913
NaeI GCCGGC 1 cut(s) 440
NciI CCSGG 3 cut(s) 327, 679, 972
NdeI CATATG 1 cut(s) 82
NdeII GATC 6 cut(s) 102, 187, 215, 280, 363, 579
NgoMIV GCCGGC 1 cut(s) 438
NlaIII CATG 3 cut(s) 194, 553, 721
NlaIV GGNNCC 2 cut(s) 838, 975
NmuCI GTSAC 2 cut(s) 136, 629
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 1 cut(s) 721
PaeR7I CTCGAG 1 cut(s) 469
PagI TCATGA 2 cut(s) 190, 549
PciI ACATGT 1 cut(s) 717
PciSI GCTCTTC 1 cut(s) 1119
PdiI GCCGGC 1 cut(s) 440
PfeI GAWTC 1 cut(s) 682
PflMI CCANNNNNTGG 1 cut(s) 294
PkrI GCNGC 6 cut(s) 46, 154, 315, 558, 1066, 1111
PleI GAGTC 2 cut(s) 325, 480
PpsI GAGTC 2 cut(s) 325, 480
PscI ACATGT 1 cut(s) 717
PshAI GACNNNNGTC 1 cut(s) 782
Psp124BI GAGCTC 1 cut(s) 390
Psp1406I AACGTT 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 854
PspGI CCWGG 1 cut(s) 854
PspN4I GGNNCC 2 cut(s) 838, 975
PspPI GGNCC 3 cut(s) 4, 974, 1055
PstI CTGCAG 1 cut(s) 1066
PsuI RGATCY 1 cut(s) 363
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 1 cut(s) 390
SapI GCTCTTC 1 cut(s) 1119
SaqAI TTAA 2 cut(s) 147, 651
SatI GCNGC 6 cut(s) 45, 153, 314, 557, 1065, 1110
Sau3AI GATC 6 cut(s) 102, 187, 215, 280, 363, 579
Sau96I GGNCC 3 cut(s) 4, 974, 1055
SchI GAGTC 2 cut(s) 325, 481
ScrFI CCNGG 4 cut(s) 327, 679, 856, 972
SduI GDGCHC 2 cut(s) 390, 642
SfaNI GCATC 4 cut(s) 52, 715, 727, 1076
SfcI CTRYAG 1 cut(s) 1062
Sfr274I CTCGAG 1 cut(s) 469
SinI GGWCC 2 cut(s) 4, 1055
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
Sse9I AATT 7 cut(s) 231, 292, 499, 545, 566, 961, 991
SsiI CCGC 6 cut(s) 42, 152, 204, 557, 816, 877
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 4 cut(s) 325, 677, 854, 970
TaaI ACNGT 1 cut(s) 925
TaiI ACGT 1 cut(s) 423
TaqI TCGA 1 cut(s) 470
TasI AATT 7 cut(s) 231, 292, 499, 545, 566, 961, 991
TauI GCSGC 2 cut(s) 155, 559
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 2 cut(s) 147, 651
Tru9I TTAA 2 cut(s) 147, 651
TscAI CASTG 3 cut(s) 274, 357, 1048
TseFI GTSAC 2 cut(s) 136, 629
TseI GCWGC 4 cut(s) 44, 313, 1064, 1109
Tsp45I GTSAC 2 cut(s) 136, 629
TspDTI ATGAA 5 cut(s) 207, 538, 566, 837, 1014
TspRI CASTG 3 cut(s) 274, 357, 1048
Van91I CCANNNNNTGG 1 cut(s) 294
VpaK11BI GGWCC 2 cut(s) 4, 1055
XapI RAATTY 4 cut(s) 499, 566, 961, 991
XceI RCATGY 1 cut(s) 721
XhoI CTCGAG 1 cut(s) 469
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.