pycom17g21580

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0015223
Physical Location & Seq
Forward (+)
19961525 .. 19963151
1627 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g21580.2

Sequence Viewer

Length: 1275 bp
ATGGCATGCCTGAAGCAAAAGTATTGCATCATCCAAAGGATCGCTTACGGGCGGGGCGGTTTCTTGACTGAGGTTGCCGTCGTTGTTGGGTTACAAGATATTTTTATTAGGTCATCTCCTACATTCCTCTTCCACAGAAGGAATGCTTTCATTGTTAATAGATCATTCACAAATCAAATGGAGCTCTCTTTCTTCTGCTTCCTTCTTCTCTTCTCCACCACTACGTTTCTCAATGTGGATGCATCTTTTACTGGAACATACGGAGTAAACTATGGCAGGATCGCTGACAATATACCTTCGCCTCACAGCGTGGTGACGCTTCTCAAAGCGGCAAAGATAAAAAACGTCAGGATATATGACGCTGATCACGAAGTCCTCACCGCCTTCAAGGGATCGGGAATTGAAATAATTGTAGGGCTTGGAAATCAGTTCCTGAAAGATATCAGCGTGAACGAGGATCGCGCCACGGATTGGATAAAAGAAAATGTACAGCCATACATTCCGGGAACGCACATTGCTGGAATAGCAGTGGGGAATGAGATATTGGGGGGAACTGATATAGAACTCTCGGAGGTTTTATTGCCTTCCGTGAAGAACGTTTACAATGCGCTTAGCAGGCTTAATCTGGCGACCTCCATTCAGGTCTCGAGTCCACATTCGGAGGCTGTGTTCGCGAATTCCTACCCGCCATCTGCCTGCATTTTCAAGGAAGATGTTGCTCAATTCATGAACCCGCTTTTGGAATTTTTCTCACAGATTAATTCCCCTTTTTACATAAATGCATATCCATTTCTGGCATATAAGAGCGACCCTGAGCACATTGACCTCAACTATGCTCTCTTCAAGAAAAATGCTGGGATTCATGATACAAAAACCAACCTACATTACGACAACATGTTTGACGCAATGGTTGATGCGGCGTATTTTGCTCTGGAGAAGGCTGGATTTGACAAGACAGAGGTCATAGTTTCCGAAACTGGTTGGGCGTCTCGCGGGGACGAAAGTGAAGCAGGAGCCAATGTCCAAAATGCCAGGACGTACAACTACAACTTGCGCAAACGGCTGTTGAAGAAGAAGGGGACGCCACATAGGCCAAAGATGGTTGTGAGAGCTTACATTTTTGCTTTGTTCAACGAGAATTTGAAGCCAGGGCCGACGTCTGAGAGGAACTTCGGATTGTTCAAAGCTGATGGGAGCATTTCATATGAAATTGGCTTCACTGGACTTGTTGGACCTTCTGCAGCATCATCATCTCTTCTTAACTACAAGGTATGA

Protein Analysis

425

Amino Acids

47.12

Weight (kDa)

7.67

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15800
fragaria_vesca FvH4_6g29810
malus_domestica MD00G1122900.v1.1 MD17G1210800.v1.1
prunus_persica Prupe.3G086200_v2.0.a1
pyrus_communis pycom09g14110 pycom17g21580
rosa_chinensis RchiOBHm_Chr2g0135631
rosa_laevigata RLG00000019473
rosa_multiflora Rmu_sc0002398.1_g000035
rosa_roxburghii Rroxscaffold_2G00109030
rosa_rugosa Rorug02G0325300
rosa_samantha Rh2AG376700 Rh2BG383000 Rh2DG399100
rosa_wichuraiana Rw2G030720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1160
Acc16I TGCGCA 1 cut(s) 1055
AccB7I CCANNNNNTGG 1 cut(s) 471
AccII CGCG 3 cut(s) 462, 674, 993
AciI CCGC 8 cut(s) 52, 57, 329, 381, 686, 734, 917, 993
AclI AACGTT 1 cut(s) 597
AclWI GGATC 4 cut(s) 47, 287, 400, 465
AcsI RAATTY 3 cut(s) 676, 743, 1138
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 3 cut(s) 986, 1082, 1157
AdeI CACNNNGTG 1 cut(s) 310
AfaI GTAC 2 cut(s) 489, 1040
AfiI CCNNNNNNNGG 2 cut(s) 471, 739
AflIII ACRYGT 1 cut(s) 894
AgsI TTSAA 8 cut(s) 388, 404, 706, 844, 1069, 1132, 1144, 1183
AjnI CCWGG 2 cut(s) 1031, 1147
AjuI GAANNNNNNNTTGG 4 cut(s) 527, 559, 722, 754
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 3 cut(s) 184, 1112, 1187
AluI AGCT 3 cut(s) 184, 1112, 1187
Alw21I GWGCWC 2 cut(s) 186, 819
Alw26I GTCTC 2 cut(s) 649, 993
AlwI GGATC 4 cut(s) 47, 287, 400, 465
AlwNI CAGNNNCTG 1 cut(s) 433
Ama87I CYCGRG 1 cut(s) 646
AoxI GGCC 2 cut(s) 1091, 1151
ApeKI GCWGC 1 cut(s) 1241
ApoI RAATTY 3 cut(s) 676, 743, 1138
ArsI GACNNNNNNTTYG 2 cut(s) 881, 913
AseI ATTAAT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 146
AspLEI GCGC 3 cut(s) 464, 610, 1056
AspS9I GGNCC 2 cut(s) 1151, 1232
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 2 cut(s) 325, 370
AvaI CYCGRG 1 cut(s) 646
AvaII GGWCC 1 cut(s) 1232
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 584, 616
Bbv12I GWGCWC 2 cut(s) 186, 819
BbvI GCAGC 1 cut(s) 1253
BccI CCATC 3 cut(s) 697, 1093, 1184
BceAI ACGGC 2 cut(s) 62, 1076
BciT130I CCWGG 2 cut(s) 1033, 1149
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 2 cut(s) 649, 993
BfmI CTRYAG 1 cut(s) 1239
BglI GCCNNNNNGGC 1 cut(s) 1090
BisI GCNGC 3 cut(s) 330, 918, 1242
BlpI GCTNAGC 1 cut(s) 611
BlsI GCNGC 3 cut(s) 331, 919, 1243
Bme1390I CCNGG 3 cut(s) 504, 1033, 1149
Bme18I GGWCC 1 cut(s) 1232
BmeT110I CYCGRG 1 cut(s) 646
BmgT120I GGNCC 2 cut(s) 1151, 1232
BmiI GGNNCC 1 cut(s) 1015
BmrFI CCNGG 3 cut(s) 504, 1033, 1149
BmsI GCATC 5 cut(s) 36, 229, 251, 904, 1253
BoxI GACNNNNGTC 1 cut(s) 959
BpmI CTGGAG 1 cut(s) 953
Bpu10I CCTNAGC 1 cut(s) 813
Bpu1102I GCTNAGC 1 cut(s) 611
BpuMI CCSGG 1 cut(s) 504
BsaHI GRCGYC 3 cut(s) 986, 1082, 1157
BsaI GGTCTC 1 cut(s) 649
BsaJI CCNNGG 2 cut(s) 465, 1148
BsaXI ACNNNNNCTCC 4 cut(s) 653, 683, 1005, 1035
Bsc4I CCNNNNNNNGG 2 cut(s) 471, 739
Bse1I ACTGG 3 cut(s) 256, 982, 1225
Bse3DI GCAATG 2 cut(s) 513, 912
BseBI CCWGG 2 cut(s) 1033, 1149
BseDI CCNNGG 2 cut(s) 465, 1148
BseGI GGATG 2 cut(s) 30, 244
BseLI CCNNNNNNNGG 2 cut(s) 471, 739
BseMI GCAATG 2 cut(s) 513, 912
BseMII CTCAG 3 cut(s) 60, 804, 1152
BseNI ACTGG 3 cut(s) 256, 982, 1225
BseXI GCAGC 1 cut(s) 1253
BseYI CCCAGC 1 cut(s) 854
Bsh1236I CGCG 3 cut(s) 462, 674, 993
BshFI GGCC 2 cut(s) 1093, 1153
BsiHKAI GWGCWC 2 cut(s) 186, 819
BsiHKCI CYCGRG 1 cut(s) 646
BsiSI CCGG 1 cut(s) 503
BslFI GGGAC 2 cut(s) 1010, 1093
BslI CCNNNNNNNGG 2 cut(s) 471, 739
BsmAI GTCTC 2 cut(s) 649, 993
BsmBI CGTCTC 1 cut(s) 993
BsmFI GGGAC 2 cut(s) 1010, 1093
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 2 cut(s) 1093, 1153
Bso31I GGTCTC 1 cut(s) 649
BsoBI CYCGRG 1 cut(s) 646
Bsp1286I GDGCHC 2 cut(s) 186, 819
Bsp1407I TGTACA 1 cut(s) 487
Bsp143I GATC 6 cut(s) 39, 161, 279, 364, 392, 457
Bsp1720I GCTNAGC 1 cut(s) 611
Bsp68I TCGCGA 1 cut(s) 674
BspACI CCGC 8 cut(s) 52, 57, 329, 381, 686, 734, 917, 993
BspANI GGCC 2 cut(s) 1093, 1153
BspCNI CTCAG 3 cut(s) 61, 805, 1153
BspFNI CGCG 3 cut(s) 462, 674, 993
BspHI TCATGA 2 cut(s) 726, 862
BspLI GGNNCC 1 cut(s) 1015
BspMAI CTGCAG 1 cut(s) 1243
BspPI GGATC 4 cut(s) 47, 287, 400, 465
BspTNI GGTCTC 1 cut(s) 649
BsrDI GCAATG 2 cut(s) 513, 912
BsrGI TGTACA 1 cut(s) 487
BsrI ACTGG 3 cut(s) 256, 982, 1225
BssECI CCNNGG 2 cut(s) 465, 1148
BssMI GATC 6 cut(s) 39, 161, 279, 364, 392, 457
BssNI GRCGYC 3 cut(s) 986, 1082, 1157
Bst2UI CCWGG 2 cut(s) 1033, 1149
Bst6I CTCTTC 4 cut(s) 134, 215, 845, 1260
BstACI GRCGYC 3 cut(s) 986, 1082, 1157
BstAUI TGTACA 1 cut(s) 487
BstC8I GCNNGC 3 cut(s) 7, 617, 697
BstDEI CTNAG 4 cut(s) 69, 611, 813, 1161
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 2 cut(s) 30, 244
BstFNI CGCG 3 cut(s) 462, 674, 993
BstHHI GCGC 3 cut(s) 464, 610, 1056
BstKTI GATC 6 cut(s) 42, 164, 282, 367, 395, 460
BstMAI GTCTC 2 cut(s) 649, 993
BstMBI GATC 6 cut(s) 39, 161, 279, 364, 392, 457
BstMWI GCNNNNNNNGC 6 cut(s) 524, 616, 671, 926, 1060, 1090
BstNI CCWGG 2 cut(s) 1033, 1149
BstNSI RCATGY 2 cut(s) 9, 898
BstPAI GACNNNNGTC 1 cut(s) 959
BstSCI CCNGG 3 cut(s) 502, 1031, 1147
BstSFI CTRYAG 1 cut(s) 1239
BstUI CGCG 3 cut(s) 462, 674, 993
BstV1I GCAGC 1 cut(s) 1253
BsuRI GGCC 2 cut(s) 1093, 1153
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 2 cut(s) 30, 244
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 534, 1218
BtuMI TCGCGA 1 cut(s) 674
Cac8I GCNNGC 3 cut(s) 7, 617, 697
CaiI CAGNNNCTG 1 cut(s) 433
CciI TCATGA 2 cut(s) 726, 862
CfoI GCGC 3 cut(s) 464, 610, 1056
Cfr13I GGNCC 2 cut(s) 1151, 1232
CseI GACGC 5 cut(s) 325, 368, 911, 975, 1090
Csp6I GTAC 2 cut(s) 488, 1039
CviAII CATG 4 cut(s) 6, 727, 863, 895
CviQI GTAC 2 cut(s) 488, 1039
DdeI CTNAG 4 cut(s) 69, 611, 813, 1161
DpnI GATC 6 cut(s) 41, 163, 281, 366, 394, 459
DpnII GATC 6 cut(s) 39, 161, 279, 364, 392, 457
DraIII CACNNNGTG 1 cut(s) 310
Eam1104I CTCTTC 4 cut(s) 134, 215, 845, 1260
EarI CTCTTC 4 cut(s) 134, 215, 845, 1260
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 649
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 1232
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 646
EcoICRI GAGCTC 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 676
EcoRII CCWGG 2 cut(s) 1031, 1147
EcoRV GATATC 1 cut(s) 442
EcoT22I ATGCAT 2 cut(s) 244, 784
EcoT38I GRGCYC 1 cut(s) 186
Esp3I CGTCTC 1 cut(s) 993
FaeI CATG 4 cut(s) 9, 730, 866, 898
FalI AAGNNNNNCTT 4 cut(s) 28, 60, 130, 162
FaqI GGGAC 2 cut(s) 1010, 1093
FatI CATG 4 cut(s) 5, 726, 862, 894
FauI CCCGC 4 cut(s) 45, 693, 741, 986
FauNDI CATATG 1 cut(s) 1204
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 3 cut(s) 330, 918, 1242
FokI GGATG 2 cut(s) 17, 251
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 330, 918, 1242
FspI TGCGCA 1 cut(s) 1055
GlaI GCGC 3 cut(s) 463, 609, 1055
GluI GCNGC 3 cut(s) 330, 918, 1242
GsaI CCCAGC 1 cut(s) 858
GsuI CTGGAG 1 cut(s) 953
HaeIII GGCC 2 cut(s) 1093, 1153
HapII CCGG 1 cut(s) 503
HgaI GACGC 5 cut(s) 325, 368, 911, 975, 1090
HhaI GCGC 3 cut(s) 464, 610, 1056
Hin1I GRCGYC 3 cut(s) 986, 1082, 1157
Hin1II CATG 4 cut(s) 9, 730, 866, 898
Hin6I GCGC 3 cut(s) 462, 608, 1054
HinP1I GCGC 3 cut(s) 462, 608, 1054
HinfI GANTC 2 cut(s) 649, 859
HpaII CCGG 1 cut(s) 503
HphI GGTGA 2 cut(s) 325, 370
Hpy166II GTNNAC 4 cut(s) 268, 451, 601, 653
Hpy188I TCNGA 5 cut(s) 571, 661, 973, 1162, 1175
Hpy8I GTNNAC 4 cut(s) 268, 451, 601, 653
Hpy99I CGWCG 2 cut(s) 83, 1159
HpyAV CCTTC 8 cut(s) 132, 212, 306, 394, 594, 931, 1069, 1245
HpyCH4IV ACGT 5 cut(s) 224, 345, 597, 1037, 1157
HpyCH4V TGCA 5 cut(s) 27, 242, 699, 782, 1241
HpyF10VI GCNNNNNNNGC 6 cut(s) 524, 616, 671, 926, 1060, 1090
HpyF3I CTNAG 4 cut(s) 69, 611, 813, 1161
HpySE526I ACGT 5 cut(s) 224, 345, 597, 1037, 1157
Hsp92I GRCGYC 3 cut(s) 986, 1082, 1157
Hsp92II CATG 4 cut(s) 9, 730, 866, 898
HspAI GCGC 3 cut(s) 462, 608, 1054
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 6 cut(s) 39, 161, 279, 364, 392, 457
LmnI GCTCC 3 cut(s) 181, 1013, 1194
Lsp1109I GCAGC 1 cut(s) 1253
LweI GCATC 5 cut(s) 36, 229, 251, 904, 1253
MaeII ACGT 5 cut(s) 224, 345, 597, 1037, 1157
MaeIII GTNAC 2 cut(s) 90, 313
MalI GATC 6 cut(s) 41, 163, 281, 366, 394, 459
MboI GATC 6 cut(s) 39, 161, 279, 364, 392, 457
MhlI GDGCHC 2 cut(s) 186, 819
MluCI AATT 8 cut(s) 399, 408, 676, 722, 743, 760, 1138, 1209
MlyI GAGTC 1 cut(s) 658
MmeI TCCRAC 1 cut(s) 1210
Mph1103I ATGCAT 2 cut(s) 244, 784
MroXI GAANNNNTTC 1 cut(s) 146
MseI TTAA 4 cut(s) 156, 621, 759, 1260
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 3 cut(s) 504, 1033, 1149
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 2 cut(s) 1033, 1149
MvnI CGCG 3 cut(s) 462, 674, 993
MwoI GCNNNNNNNGC 6 cut(s) 524, 616, 671, 926, 1060, 1090
NciI CCSGG 1 cut(s) 504
NdeI CATATG 1 cut(s) 1204
NdeII GATC 6 cut(s) 39, 161, 279, 364, 392, 457
NlaIII CATG 4 cut(s) 9, 730, 866, 898
NlaIV GGNNCC 1 cut(s) 1015
NmuCI GTSAC 1 cut(s) 313
NruI TCGCGA 1 cut(s) 674
NsbI TGCGCA 1 cut(s) 1055
NsiI ATGCAT 2 cut(s) 244, 784
NspI RCATGY 2 cut(s) 9, 898
PaeI GCATGC 1 cut(s) 9
PaeR7I CTCGAG 1 cut(s) 646
PagI TCATGA 2 cut(s) 726, 862
PciI ACATGT 1 cut(s) 894
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PctI GAATGC 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 1 cut(s) 859
PflMI CCANNNNNTGG 1 cut(s) 471
PfoI TCCNGGA 1 cut(s) 502
PkrI GCNGC 3 cut(s) 331, 919, 1243
PleI GAGTC 1 cut(s) 657
PpsI GAGTC 1 cut(s) 657
PscI ACATGT 1 cut(s) 894
PshAI GACNNNNGTC 1 cut(s) 959
PshBI ATTAAT 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 186
Psp1406I AACGTT 1 cut(s) 597
Psp6I CCWGG 2 cut(s) 1031, 1147
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 2 cut(s) 1031, 1147
PspN4I GGNNCC 1 cut(s) 1015
PspPI GGNCC 2 cut(s) 1151, 1232
PstI CTGCAG 1 cut(s) 1243
PstNI CAGNNNCTG 1 cut(s) 433
RruI TCGCGA 1 cut(s) 674
RsaI GTAC 2 cut(s) 489, 1040
RsaNI GTAC 2 cut(s) 488, 1039
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 4 cut(s) 156, 621, 759, 1260
SatI GCNGC 3 cut(s) 330, 918, 1242
Sau3AI GATC 6 cut(s) 39, 161, 279, 364, 392, 457
Sau96I GGNCC 2 cut(s) 1151, 1232
SchI GAGTC 1 cut(s) 658
ScrFI CCNGG 3 cut(s) 504, 1033, 1149
SduI GDGCHC 2 cut(s) 186, 819
SfaNI GCATC 5 cut(s) 36, 229, 251, 904, 1253
SfcI CTRYAG 1 cut(s) 1239
Sfr274I CTCGAG 1 cut(s) 646
SinI GGWCC 1 cut(s) 1232
SlaI CTCGAG 1 cut(s) 646
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
SphI GCATGC 1 cut(s) 9
Sse9I AATT 8 cut(s) 399, 408, 676, 722, 743, 760, 1138, 1209
SsiI CCGC 8 cut(s) 52, 57, 329, 381, 686, 734, 917, 993
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 3 cut(s) 502, 1031, 1147
TaiI ACGT 5 cut(s) 227, 348, 600, 1040, 1160
TaqI TCGA 1 cut(s) 647
TasI AATT 8 cut(s) 399, 408, 676, 722, 743, 760, 1138, 1209
TatI WGTACW 1 cut(s) 487
TauI GCSGC 2 cut(s) 332, 920
TfiI GAWTC 1 cut(s) 859
Tru1I TTAA 4 cut(s) 156, 621, 759, 1260
Tru9I TTAA 4 cut(s) 156, 621, 759, 1260
TscAI CASTG 2 cut(s) 534, 1225
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 1 cut(s) 1241
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 6 cut(s) 139, 715, 743, 851, 1191, 1221
TspGWI ACGGA 3 cut(s) 276, 482, 577
TspRI CASTG 2 cut(s) 534, 1225
Van91I CCANNNNNTGG 1 cut(s) 471
VpaK11BI GGWCC 1 cut(s) 1232
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 3 cut(s) 676, 743, 1138
XceI RCATGY 2 cut(s) 9, 898
XhoI CTCGAG 1 cut(s) 646
XmnI GAANNNNTTC 1 cut(s) 146
ZraI GACGTC 1 cut(s) 1158
Zsp2I ATGCAT 2 cut(s) 244, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.