MD17G1210800.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015223
Physical Location & Seq
Forward (+)
25562984 .. 25564896
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1210800.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGGCATGCCTGAAGCAAAAGTATTGCATCATCCAAAGGATCGCTTATGGGCGGGGCGGTTTCTTGACTGAGGTTGCCGTCGTTGTTGGGTTACAAGATATTATTATTAGGTCATCTCCAACATTCATTTTCCACAAAAGGAATGCTTTCATTGTTAATAGATCATTCACAAATCAAATGGAGCTCTCTTTCTTCTGCTTGCTTCTTCTCTTCTCCACCACTACGTTTCTCAATGTGGATGCATCTTTTACCGGAACATACGGAGTAAACTATGGCAGGCTCGCTGACAATATACCGTCGCCTCACAGCGTGGTGACGCTTCTCAAAGCGGCAAAGATAAAAAACGTCAGGATATATGACGCTGATCATGAAGTCCTCACCGCCTTCAAGGGATCGGGAATTGAAATAATTGTAGGGCTTGGAAGTCAGTTCCTGAAAGATATCAGCGTGAACGAGGATCGCGCCACGGATTGGATAAAAGAAAATGTACAGCCATACATTCCGGGAACGCACATTACTGGAATAGCAGTGGGGAATGAGATATTGGGGGGAACTGATATAGAACTCTCCGAGGTCTTATTGCCTGCCGTGAAGAACGTTTACAATGCGCTTAGCAGGCTTAATATGGCGACCTCCATTCAGGTGTCGAGTCCACATTCGGAGGCTGTGTTCGCGAATTCTTACCCGCCATCTGCCTGCATTTTCAAGGAAGATGTTGCTCAATTCATGAACCCGCTTTTGGAATTTTTCTCACAGATTAATTCCCCTTTTTACATAAATGCATATCCATTTCTGGCATATAAGAGCGACCCCGAGCACATTGACCTCAACTATGCTCTCTTCAAGAAAAATGCTGGGATTCATGATACAAAAACCAACCTGCATTACGACAACATGTTTGACGCAATGGTTGATGCGGCGTATTTTGCTCTGGAGAAGGCTGGATTTGACAAGACAGAGGTCATAGTTTCCGAAACTGGTTGGGCGTCTCGCGGGGACGAAAGTGAAGCAGGAGCCAATGTCCAAAATGCCAGGACGTACAACTACAACTTGCGCAAACGGCTGTTGAAGAAGAGGGGGACGCCACATAGGCCAAAGATGGTTGTGAGAGCTTACATTTTTGCTTTGTTCAATGAGAATTTGAAGCCGGGGCCGACGTCTGAGAGGAACTTCGGATTGTTCAAAGCTGATGGGAGCATTTCATATAACATTGGCTTCACTGGACTTGTTGGACCTTCTGCAGCATCATCATCTCTTCTTAACTACAAGGTTTTCACAAGTTGTGCAGCTCTTCTGCTTCTATTGTTAACTTCATAA

Protein Analysis

439

Amino Acids

48.49

Weight (kDa)

8.12

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 88 - 406 1.4e-84 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15800
fragaria_vesca FvH4_6g29810
malus_domestica MD00G1122900.v1.1 MD17G1210800.v1.1
prunus_persica Prupe.3G086200_v2.0.a1
pyrus_communis pycom09g14110 pycom17g21580
rosa_chinensis RchiOBHm_Chr2g0135631
rosa_laevigata RLG00000019473
rosa_multiflora Rmu_sc0002398.1_g000035
rosa_roxburghii Rroxscaffold_2G00109030
rosa_rugosa Rorug02G0325300
rosa_samantha Rh2AG376700 Rh2BG383000 Rh2DG399100
rosa_wichuraiana Rw2G030720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1160
Acc16I TGCGCA 1 cut(s) 1055
Acc36I ACCTGC 1 cut(s) 888
AccB7I CCANNNNNTGG 1 cut(s) 471
AccII CGCG 3 cut(s) 462, 674, 993
AciI CCGC 8 cut(s) 52, 57, 329, 381, 686, 734, 917, 993
AclI AACGTT 1 cut(s) 597
AclWI GGATC 3 cut(s) 47, 400, 465
AcsI RAATTY 3 cut(s) 676, 743, 1138
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 3 cut(s) 986, 1082, 1157
AdeI CACNNNGTG 1 cut(s) 310
AfaI GTAC 2 cut(s) 489, 1040
AfiI CCNNNNNNNGG 2 cut(s) 471, 739
AflIII ACRYGT 1 cut(s) 894
AgsI TTSAA 8 cut(s) 388, 404, 706, 844, 1069, 1132, 1144, 1183
AjnI CCWGG 1 cut(s) 1031
AjuI GAANNNNNNNTTGG 4 cut(s) 527, 559, 722, 754
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 4 cut(s) 184, 1112, 1187, 1289
AluI AGCT 4 cut(s) 184, 1112, 1187, 1289
Alw21I GWGCWC 2 cut(s) 186, 819
Alw26I GTCTC 1 cut(s) 993
AlwI GGATC 3 cut(s) 47, 400, 465
AlwNI CAGNNNCTG 1 cut(s) 433
Ama87I CYCGRG 1 cut(s) 812
AoxI GGCC 2 cut(s) 1091, 1151
ApeKI GCWGC 2 cut(s) 1241, 1286
ApoI RAATTY 3 cut(s) 676, 743, 1138
ArsI GACNNNNNNTTYG 2 cut(s) 881, 913
AseI ATTAAT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 146
AspLEI GCGC 3 cut(s) 464, 610, 1056
AspS9I GGNCC 2 cut(s) 1151, 1232
AsuC2I CCSGG 2 cut(s) 504, 1149
AsuHPI GGTGA 2 cut(s) 325, 370
AvaI CYCGRG 1 cut(s) 812
AvaII GGWCC 1 cut(s) 1232
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 584, 616
Bbv12I GWGCWC 2 cut(s) 186, 819
BbvI GCAGC 2 cut(s) 1253, 1298
BccI CCATC 3 cut(s) 697, 1093, 1184
BceAI ACGGC 3 cut(s) 62, 572, 1076
BciT130I CCWGG 1 cut(s) 1033
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 2 cut(s) 504, 1149
BcoDI GTCTC 1 cut(s) 993
BfmI CTRYAG 1 cut(s) 1239
BfuAI ACCTGC 1 cut(s) 888
BglI GCCNNNNNGGC 1 cut(s) 1090
BisI GCNGC 4 cut(s) 330, 918, 1242, 1287
BlpI GCTNAGC 1 cut(s) 611
BlsI GCNGC 4 cut(s) 331, 919, 1243, 1288
Bme1390I CCNGG 3 cut(s) 504, 1033, 1149
Bme18I GGWCC 1 cut(s) 1232
BmeT110I CYCGRG 1 cut(s) 812
BmgT120I GGNCC 2 cut(s) 1151, 1232
BmiI GGNNCC 2 cut(s) 1015, 1152
BmrFI CCNGG 3 cut(s) 504, 1033, 1149
BmsI GCATC 5 cut(s) 36, 229, 251, 904, 1253
BoxI GACNNNNGTC 1 cut(s) 959
BpmI CTGGAG 1 cut(s) 953
Bpu1102I GCTNAGC 1 cut(s) 611
BpuMI CCSGG 2 cut(s) 504, 1149
BsaHI GRCGYC 3 cut(s) 986, 1082, 1157
BsaJI CCNNGG 3 cut(s) 465, 570, 1148
BsaWI WCCGGW 1 cut(s) 251
BsaXI ACNNNNNCTCC 4 cut(s) 653, 683, 1005, 1035
Bsc4I CCNNNNNNNGG 2 cut(s) 471, 739
Bse1I ACTGG 3 cut(s) 523, 982, 1225
Bse3DI GCAATG 1 cut(s) 912
BseBI CCWGG 1 cut(s) 1033
BseDI CCNNGG 3 cut(s) 465, 570, 1148
BseGI GGATG 2 cut(s) 30, 244
BseLI CCNNNNNNNGG 2 cut(s) 471, 739
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 2 cut(s) 60, 1152
BseNI ACTGG 3 cut(s) 523, 982, 1225
BseXI GCAGC 2 cut(s) 1253, 1298
BseYI CCCAGC 1 cut(s) 854
BsgI GTGCAG 1 cut(s) 1305
Bsh1236I CGCG 3 cut(s) 462, 674, 993
BshFI GGCC 2 cut(s) 1093, 1153
BsiHKAI GWGCWC 2 cut(s) 186, 819
BsiHKCI CYCGRG 1 cut(s) 812
BsiSI CCGG 3 cut(s) 252, 503, 1148
BslFI GGGAC 2 cut(s) 1010, 1093
BslI CCNNNNNNNGG 2 cut(s) 471, 739
BsmAI GTCTC 1 cut(s) 993
BsmBI CGTCTC 1 cut(s) 993
BsmFI GGGAC 2 cut(s) 1010, 1093
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 2 cut(s) 1093, 1153
BsoBI CYCGRG 1 cut(s) 812
Bsp1286I GDGCHC 2 cut(s) 186, 819
Bsp1407I TGTACA 1 cut(s) 487
Bsp143I GATC 5 cut(s) 39, 161, 364, 392, 457
Bsp1720I GCTNAGC 1 cut(s) 611
Bsp68I TCGCGA 1 cut(s) 674
BspACI CCGC 8 cut(s) 52, 57, 329, 381, 686, 734, 917, 993
BspANI GGCC 2 cut(s) 1093, 1153
BspCNI CTCAG 2 cut(s) 61, 1153
BspFNI CGCG 3 cut(s) 462, 674, 993
BspHI TCATGA 3 cut(s) 367, 726, 862
BspLI GGNNCC 2 cut(s) 1015, 1152
BspMAI CTGCAG 1 cut(s) 1243
BspMI ACCTGC 1 cut(s) 888
BspPI GGATC 3 cut(s) 47, 400, 465
BspQI GCTCTTC 1 cut(s) 1296
BsrDI GCAATG 1 cut(s) 912
BsrGI TGTACA 1 cut(s) 487
BsrI ACTGG 3 cut(s) 523, 982, 1225
BssECI CCNNGG 3 cut(s) 465, 570, 1148
BssMI GATC 5 cut(s) 39, 161, 364, 392, 457
BssNI GRCGYC 3 cut(s) 986, 1082, 1157
Bst2UI CCWGG 1 cut(s) 1033
Bst4CI ACNGT 1 cut(s) 297
Bst6I CTCTTC 5 cut(s) 215, 845, 1067, 1260, 1296
BstACI GRCGYC 3 cut(s) 986, 1082, 1157
BstAUI TGTACA 1 cut(s) 487
BstC8I GCNNGC 7 cut(s) 7, 200, 278, 282, 585, 617, 697
BstDEI CTNAG 3 cut(s) 69, 611, 1161
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 2 cut(s) 30, 244
BstFNI CGCG 3 cut(s) 462, 674, 993
BstHHI GCGC 3 cut(s) 464, 610, 1056
BstKTI GATC 5 cut(s) 42, 164, 367, 395, 460
BstMAI GTCTC 1 cut(s) 993
BstMBI GATC 5 cut(s) 39, 161, 364, 392, 457
BstMWI GCNNNNNNNGC 5 cut(s) 616, 671, 926, 1060, 1090
BstNI CCWGG 1 cut(s) 1033
BstNSI RCATGY 2 cut(s) 9, 898
BstPAI GACNNNNGTC 1 cut(s) 959
BstSCI CCNGG 3 cut(s) 502, 1031, 1147
BstSFI CTRYAG 1 cut(s) 1239
BstUI CGCG 3 cut(s) 462, 674, 993
BstV1I GCAGC 2 cut(s) 1253, 1298
BsuRI GGCC 2 cut(s) 1093, 1153
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 2 cut(s) 30, 244
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 534, 1218
BtuMI TCGCGA 1 cut(s) 674
BveI ACCTGC 1 cut(s) 888
Cac8I GCNNGC 7 cut(s) 7, 200, 278, 282, 585, 617, 697
CaiI CAGNNNCTG 1 cut(s) 433
CciI TCATGA 3 cut(s) 367, 726, 862
CfoI GCGC 3 cut(s) 464, 610, 1056
Cfr13I GGNCC 2 cut(s) 1151, 1232
CseI GACGC 5 cut(s) 325, 368, 911, 975, 1090
Csp6I GTAC 2 cut(s) 488, 1039
CviAII CATG 5 cut(s) 6, 368, 727, 863, 895
CviQI GTAC 2 cut(s) 488, 1039
DdeI CTNAG 3 cut(s) 69, 611, 1161
DpnI GATC 5 cut(s) 41, 163, 366, 394, 459
DpnII GATC 5 cut(s) 39, 161, 364, 392, 457
DraIII CACNNNGTG 1 cut(s) 310
Eam1104I CTCTTC 5 cut(s) 215, 845, 1067, 1260, 1296
EarI CTCTTC 5 cut(s) 215, 845, 1067, 1260, 1296
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 1232
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 812
EcoICRI GAGCTC 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 676
EcoRII CCWGG 1 cut(s) 1031
EcoRV GATATC 1 cut(s) 442
EcoT22I ATGCAT 2 cut(s) 244, 784
EcoT38I GRGCYC 1 cut(s) 186
Esp3I CGTCTC 1 cut(s) 993
FaeI CATG 5 cut(s) 9, 371, 730, 866, 898
FalI AAGNNNNNCTT 4 cut(s) 28, 60, 130, 162
FaqI GGGAC 2 cut(s) 1010, 1093
FatI CATG 5 cut(s) 5, 367, 726, 862, 894
FauI CCCGC 4 cut(s) 45, 693, 741, 986
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 4 cut(s) 330, 918, 1242, 1287
FokI GGATG 2 cut(s) 17, 251
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 4 cut(s) 330, 918, 1242, 1287
FspI TGCGCA 1 cut(s) 1055
GlaI GCGC 3 cut(s) 463, 609, 1055
GluI GCNGC 4 cut(s) 330, 918, 1242, 1287
GsaI CCCAGC 1 cut(s) 858
GsuI CTGGAG 1 cut(s) 953
HaeIII GGCC 2 cut(s) 1093, 1153
HapII CCGG 3 cut(s) 252, 503, 1148
HgaI GACGC 5 cut(s) 325, 368, 911, 975, 1090
HhaI GCGC 3 cut(s) 464, 610, 1056
Hin1I GRCGYC 3 cut(s) 986, 1082, 1157
Hin1II CATG 5 cut(s) 9, 371, 730, 866, 898
Hin6I GCGC 3 cut(s) 462, 608, 1054
HinP1I GCGC 3 cut(s) 462, 608, 1054
HincII GTYRAC 1 cut(s) 1308
HindII GTYRAC 1 cut(s) 1308
HinfI GANTC 2 cut(s) 649, 859
HpaI GTTAAC 1 cut(s) 1308
HpaII CCGG 3 cut(s) 252, 503, 1148
HphI GGTGA 2 cut(s) 325, 370
Hpy166II GTNNAC 5 cut(s) 268, 451, 601, 653, 1308
Hpy188I TCNGA 5 cut(s) 571, 661, 973, 1162, 1175
Hpy8I GTNNAC 5 cut(s) 268, 451, 601, 653, 1308
Hpy99I CGWCG 3 cut(s) 83, 301, 1159
HpyAV CCTTC 3 cut(s) 394, 931, 1245
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4IV ACGT 5 cut(s) 224, 345, 597, 1037, 1157
HpyCH4V TGCA 7 cut(s) 27, 242, 699, 782, 883, 1241, 1286
HpyF10VI GCNNNNNNNGC 5 cut(s) 616, 671, 926, 1060, 1090
HpyF3I CTNAG 3 cut(s) 69, 611, 1161
HpySE526I ACGT 5 cut(s) 224, 345, 597, 1037, 1157
Hsp92I GRCGYC 3 cut(s) 986, 1082, 1157
Hsp92II CATG 5 cut(s) 9, 371, 730, 866, 898
HspAI GCGC 3 cut(s) 462, 608, 1054
Ksp22I TGATCA 1 cut(s) 364
KspAI GTTAAC 1 cut(s) 1308
Kzo9I GATC 5 cut(s) 39, 161, 364, 392, 457
LguI GCTCTTC 1 cut(s) 1296
LmnI GCTCC 3 cut(s) 181, 1013, 1194
Lsp1109I GCAGC 2 cut(s) 1253, 1298
LweI GCATC 5 cut(s) 36, 229, 251, 904, 1253
MaeII ACGT 5 cut(s) 224, 345, 597, 1037, 1157
MaeIII GTNAC 2 cut(s) 90, 313
MalI GATC 5 cut(s) 41, 163, 366, 394, 459
MboI GATC 5 cut(s) 39, 161, 364, 392, 457
MhlI GDGCHC 2 cut(s) 186, 819
MluCI AATT 7 cut(s) 399, 408, 676, 722, 743, 760, 1138
MlyI GAGTC 1 cut(s) 658
MmeI TCCRAC 2 cut(s) 143, 1210
Mph1103I ATGCAT 2 cut(s) 244, 784
MroXI GAANNNNTTC 1 cut(s) 146
MseI TTAA 5 cut(s) 156, 621, 759, 1260, 1307
MslI CAYNNNNRTG 1 cut(s) 641
MspI CCGG 3 cut(s) 252, 503, 1148
MspR9I CCNGG 3 cut(s) 504, 1033, 1149
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 1 cut(s) 1033
MvnI CGCG 3 cut(s) 462, 674, 993
MwoI GCNNNNNNNGC 5 cut(s) 616, 671, 926, 1060, 1090
NciI CCSGG 2 cut(s) 504, 1149
NdeII GATC 5 cut(s) 39, 161, 364, 392, 457
NlaIII CATG 5 cut(s) 9, 371, 730, 866, 898
NlaIV GGNNCC 2 cut(s) 1015, 1152
NmuCI GTSAC 1 cut(s) 313
NruI TCGCGA 1 cut(s) 674
NsbI TGCGCA 1 cut(s) 1055
NsiI ATGCAT 2 cut(s) 244, 784
NspI RCATGY 2 cut(s) 9, 898
PaeI GCATGC 1 cut(s) 9
PagI TCATGA 3 cut(s) 367, 726, 862
PciI ACATGT 1 cut(s) 894
PciSI GCTCTTC 1 cut(s) 1296
PctI GAATGC 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 1 cut(s) 859
PflMI CCANNNNNTGG 1 cut(s) 471
PfoI TCCNGGA 1 cut(s) 502
PkrI GCNGC 4 cut(s) 331, 919, 1243, 1288
PleI GAGTC 1 cut(s) 657
PpsI GAGTC 1 cut(s) 657
PscI ACATGT 1 cut(s) 894
PshAI GACNNNNGTC 1 cut(s) 959
PshBI ATTAAT 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 186
Psp1406I AACGTT 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 1031
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 1 cut(s) 1031
PspN4I GGNNCC 2 cut(s) 1015, 1152
PspPI GGNCC 2 cut(s) 1151, 1232
PsrI GAACNNNNNNTAC 2 cut(s) 499, 531
PstI CTGCAG 1 cut(s) 1243
PstNI CAGNNNCTG 1 cut(s) 433
RruI TCGCGA 1 cut(s) 674
RsaI GTAC 2 cut(s) 489, 1040
RsaNI GTAC 2 cut(s) 488, 1039
RseI CAYNNNNRTG 1 cut(s) 641
SacI GAGCTC 1 cut(s) 186
SapI GCTCTTC 1 cut(s) 1296
SaqAI TTAA 5 cut(s) 156, 621, 759, 1260, 1307
SatI GCNGC 4 cut(s) 330, 918, 1242, 1287
Sau3AI GATC 5 cut(s) 39, 161, 364, 392, 457
Sau96I GGNCC 2 cut(s) 1151, 1232
SchI GAGTC 1 cut(s) 658
ScrFI CCNGG 3 cut(s) 504, 1033, 1149
SduI GDGCHC 2 cut(s) 186, 819
SfaNI GCATC 5 cut(s) 36, 229, 251, 904, 1253
SfcI CTRYAG 1 cut(s) 1239
SinI GGWCC 1 cut(s) 1232
SmiMI CAYNNNNRTG 1 cut(s) 641
SphI GCATGC 1 cut(s) 9
Sse9I AATT 7 cut(s) 399, 408, 676, 722, 743, 760, 1138
SsiI CCGC 8 cut(s) 52, 57, 329, 381, 686, 734, 917, 993
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 3 cut(s) 502, 1031, 1147
TaaI ACNGT 1 cut(s) 297
TaiI ACGT 5 cut(s) 227, 348, 600, 1040, 1160
TaqI TCGA 1 cut(s) 647
TasI AATT 7 cut(s) 399, 408, 676, 722, 743, 760, 1138
TatI WGTACW 1 cut(s) 487
TauI GCSGC 2 cut(s) 332, 920
TfiI GAWTC 1 cut(s) 859
Tru1I TTAA 5 cut(s) 156, 621, 759, 1260, 1307
Tru9I TTAA 5 cut(s) 156, 621, 759, 1260, 1307
TscAI CASTG 2 cut(s) 534, 1225
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 2 cut(s) 1241, 1286
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 8 cut(s) 115, 139, 384, 715, 743, 851, 1191, 1302
TspGWI ACGGA 2 cut(s) 276, 482
TspRI CASTG 2 cut(s) 534, 1225
Van91I CCANNNNNTGG 1 cut(s) 471
VpaK11BI GGWCC 1 cut(s) 1232
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 3 cut(s) 676, 743, 1138
XceI RCATGY 2 cut(s) 9, 898
XmnI GAANNNNTTC 1 cut(s) 146
ZraI GACGTC 1 cut(s) 1158
Zsp2I ATGCAT 2 cut(s) 244, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.