Rorug02G0325300

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015223
Physical Location & Seq
Forward (+)
39902894 .. 39903217
324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0325300.1

Sequence Viewer

Length: 324 bp
ATGATATTTGCACTAATTTGTGGATCGGCCATGGGTCAAGCGTCCAGTTTCTGTGCTCAAGCCTGGAACGAGTTCAGCAACTGCATGAGGTTCATCACCGGGTTATTTTCGCAACCGTCAACGCAGTGCTGTGATTCGGTGAGGAGATTGAACAAGATGGCCAAAAGTAGTGAGAACGGACCTCGCGATATATGTCAATGCATTGAGGACATGTCTCGTACATATGGTTTTCCTTTTGTAGCCTCCATCATCCAAGATCTACCCATCATATGCAATGCGCACCTTAGTTTCCCAATCTCTAATCGCATGAACTGCACCATGTAA

Protein Analysis

107

Amino Acids

11.91

Weight (kDa)

7.49

Isoelectric Point (pI)

55.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 18 - 105 2.5e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15800
fragaria_vesca FvH4_6g29810
malus_domestica MD00G1122900.v1.1 MD17G1210800.v1.1
prunus_persica Prupe.3G086200_v2.0.a1
pyrus_communis pycom09g14110 pycom17g21580
rosa_chinensis RchiOBHm_Chr2g0135631
rosa_laevigata RLG00000019473
rosa_multiflora Rmu_sc0002398.1_g000035
rosa_roxburghii Rroxscaffold_2G00109030
rosa_rugosa Rorug02G0325300
rosa_samantha Rh2AG376700 Rh2BG383000 Rh2DG399100
rosa_wichuraiana Rw2G030720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 279
AccII CGCG 1 cut(s) 186
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 2 cut(s) 27, 159
AfaI GTAC 1 cut(s) 220
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 1 cut(s) 62
Alw21I GWGCWC 1 cut(s) 58
Alw26I GTCTC 1 cut(s) 219
AlwI GGATC 1 cut(s) 31
AlwNI CAGNNNCTG 2 cut(s) 51, 81
AoxI GGCC 2 cut(s) 27, 159
Asp700I GAANNNNTTC 1 cut(s) 71
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 1 cut(s) 179
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 2 cut(s) 88, 151
AvaII GGWCC 1 cut(s) 179
BalI TGGCCA 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 58
BccI CCATC 3 cut(s) 151, 254, 272
BciT130I CCWGG 1 cut(s) 64
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 219
BglII AGATCT 1 cut(s) 256
Bme1390I CCNGG 2 cut(s) 64, 100
Bme18I GGWCC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 179
BmrFI CCNGG 2 cut(s) 64, 100
BpuEI CTTGAG 1 cut(s) 42
BpuMI CCSGG 1 cut(s) 100
BsaJI CCNNGG 1 cut(s) 30
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 249
BseMI GCAATG 1 cut(s) 280
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 1 cut(s) 157
BsgI GTGCAG 1 cut(s) 298
Bsh1236I CGCG 1 cut(s) 186
BshFI GGCC 2 cut(s) 29, 161
BsiHKAI GWGCWC 1 cut(s) 58
BsiSI CCGG 1 cut(s) 99
BsmAI GTCTC 1 cut(s) 219
BsnI GGCC 2 cut(s) 29, 161
Bsp1286I GDGCHC 1 cut(s) 58
Bsp143I GATC 2 cut(s) 23, 256
Bsp19I CCATGG 1 cut(s) 30
Bsp68I TCGCGA 1 cut(s) 186
BspANI GGCC 2 cut(s) 29, 161
BspFNI CGCG 1 cut(s) 186
BspPI GGATC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 280
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 2 cut(s) 23, 256
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 1 cut(s) 117
BstAPI GCANNNNNTGC 1 cut(s) 312
BstDEI CTNAG 1 cut(s) 284
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 249
BstFNI CGCG 1 cut(s) 186
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 2 cut(s) 26, 259
BstMAI GTCTC 1 cut(s) 219
BstMBI GATC 2 cut(s) 23, 256
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 2 cut(s) 62, 98
BstUI CGCG 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 2 cut(s) 29, 161
BtgI CCRYGG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 249
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 1 cut(s) 131
BtuMI TCGCGA 1 cut(s) 186
CaiI CAGNNNCTG 2 cut(s) 51, 81
CfoI GCGC 1 cut(s) 280
Cfr13I GGNCC 1 cut(s) 179
CseI GACGC 1 cut(s) 30
Csp6I GTAC 1 cut(s) 219
CviAII CATG 5 cut(s) 31, 85, 211, 307, 319
CviJI RGCY 4 cut(s) 29, 62, 161, 242
CviKI_1 RGCY 4 cut(s) 29, 62, 161, 242
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 1 cut(s) 284
DpnI GATC 2 cut(s) 25, 258
DpnII GATC 2 cut(s) 23, 256
EaeI YGGCCR 2 cut(s) 27, 159
Eco130I CCWWGG 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 203
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 5 cut(s) 34, 88, 214, 310, 322
FatI CATG 5 cut(s) 30, 84, 210, 306, 318
FauNDI CATATG 2 cut(s) 223, 269
FokI GGATG 1 cut(s) 236
FspAI RTGCGCAY 1 cut(s) 279
FspI TGCGCA 1 cut(s) 279
GlaI GCGC 1 cut(s) 279
HaeIII GGCC 2 cut(s) 29, 161
HapII CCGG 1 cut(s) 99
HgaI GACGC 1 cut(s) 30
HhaI GCGC 1 cut(s) 280
Hin1II CATG 5 cut(s) 34, 88, 214, 310, 322
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HinfI GANTC 1 cut(s) 134
HpaII CCGG 1 cut(s) 99
HphI GGTGA 2 cut(s) 88, 151
Hpy166II GTNNAC 1 cut(s) 120
Hpy188III TCNNGA 1 cut(s) 185
Hpy8I GTNNAC 1 cut(s) 120
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4V TGCA 5 cut(s) 11, 84, 201, 273, 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 1 cut(s) 284
Hsp92II CATG 5 cut(s) 34, 88, 214, 310, 322
HspAI GCGC 1 cut(s) 278
Kzo9I GATC 2 cut(s) 23, 256
LpnPI CCDG 4 cut(s) 49, 58, 76, 112
MalI GATC 2 cut(s) 25, 258
MboI GATC 2 cut(s) 23, 256
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 58
MlsI TGGCCA 1 cut(s) 161
MluCI AATT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 161
MnlI CCTC 5 cut(s) 81, 135, 192, 199, 253
Mox20I TGGCCA 1 cut(s) 161
Mph1103I ATGCAT 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 71
MscI TGGCCA 1 cut(s) 161
Msp20I TGGCCA 1 cut(s) 161
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 2 cut(s) 64, 100
MvaI CCWGG 1 cut(s) 64
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 312
NciI CCSGG 1 cut(s) 100
NcoI CCATGG 1 cut(s) 30
NdeI CATATG 2 cut(s) 223, 269
NdeII GATC 2 cut(s) 23, 256
NlaIII CATG 5 cut(s) 34, 88, 214, 310, 322
NruI TCGCGA 1 cut(s) 186
NsbI TGCGCA 1 cut(s) 279
NsiI ATGCAT 1 cut(s) 203
NspI RCATGY 1 cut(s) 214
PciI ACATGT 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 134
PscI ACATGT 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspPI GGNCC 1 cut(s) 179
PstNI CAGNNNCTG 2 cut(s) 51, 81
PsuI RGATCY 1 cut(s) 256
RruI TCGCGA 1 cut(s) 186
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
Sau3AI GATC 2 cut(s) 23, 256
Sau96I GGNCC 1 cut(s) 179
ScrFI CCNGG 2 cut(s) 64, 100
SduI GDGCHC 1 cut(s) 58
SetI ASST 3 cut(s) 92, 184, 285
SinI GGWCC 1 cut(s) 179
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 1 cut(s) 15
StyD4I CCNGG 2 cut(s) 62, 98
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 1 cut(s) 117
TasI AATT 1 cut(s) 15
TfiI GAWTC 1 cut(s) 134
TscAI CASTG 1 cut(s) 131
TspDTI ATGAA 2 cut(s) 82, 323
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 179
XceI RCATGY 1 cut(s) 214
XmnI GAANNNNTTC 1 cut(s) 71
Zsp2I ATGCAT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.