Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0011504

Physical Location & Seq

Genomic Coordinates Forward (+)

26940750 .. 26943708

Gene Structure 2959 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1127000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGGTGAGGGCGTCCGTCTCAGTAATCATATACATAGCAATTGCAGTCCTTGTCCTCTTGCTCATCTCCCACTCCCCAAAGAAACCCGGCAACCACCGCCACCGCCGCCTCAAGCTCCGCTCCAACTTCACCTTTGCTCCCCCCCTCCACCACCATAACCCCATCCCCTTCGACCCCCTCGTCGCTGACATTGAGCGCCGCCGCGAGGACCGTAAGTGGGAGCAGCAGCACTTCGATCATGCCTTCAACGCCTCCCACGACTCCGCACCGGCCGCCGAGTCTCAGCCCGAGTGGGAAGACTTCATGGACGCTGAGGATTATTTGAACAATGAGGACAGGTTCAACGTCACCAACAGGTTGCTCTTGTTGTTTCCAAAGGTTGATGTCGACCCGGCTGATGGATTCGTCACCGAGGACGAGTTGACTCAGTGGAACTTGAACCAGGCTCAGAGGGAAGTCTTGCATCGAACTCAGAGGGATATGGAGCTTCACGACAAGAATAGTGACGGCTTTGTTTCCTTTGCCGAGTATCAGCCCCCCAGTTGGGTTCAGAATGCAGATAACAGCTCCTTTGGCTATGATATGGGTTGGTGGAAAGAGGAACATTTTAATGCATCCGATGCGAATGGAGATGGTCTTCTTAATATAACCGAGTTCAATGACTTTCTACACCCAGCTGATAGCAAAAACCTGAAGCTACTTAAGTGGTTGTGTAAGGAGGAAGTAAGGGAAAGGGACACCGACAAAGATGAAAAGATTAACTTCAAAGAATTCTTCCATGGCCTCTTTGATTTGGTGAGGAACTATGATGAAGAAAATCACAATTCCACACATCAGTCTGATGATTTGATGGAGTTCCCAGCTAGAAATTTGTTTGCTCAGCTTGACAAAGATGGCGATGGGTATTTGTCAGATGAGGAAATGCTTCCTATTATTGGAAAGATACATCCATCAGAACATTACTATGCAAAACAACAAGCAGATTACATTATATCACAGGCTGATACAGATAAAGATGGGCAACTAACGCTGGGAGAGATGATAGAGAACCCATATGTATTTTACAGCGCTATTTTCAATGATGATGAGGAAGAGGACTATTACCATGATGAGTTCCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005794 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0019538 GO:0031974 GO:0036211 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0070013 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

374

Amino Acids

43.8

Weight (kDa)

4.71

Isoelectric Point (pI)

42.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	288 - 349	5.7e-09	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011504)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08580 AT5G08580
fragaria_vesca	FvH4_4g37230
malus_domestica	MD00G1127000.v1.1 MD13G1002500.v1.1
prunus_persica	Prupe.1G349900_v2.0.a1
pyrus_communis	pycom13g00210 pycom16g00130
rosa_chinensis	RchiOBHm_Chr4g0447021
rosa_laevigata	RLG00000005612
rosa_multiflora	Rmu_co8164088.1_g000001 Rmu_sc0001753.1_g000001 Rmu_sc0019356.1_g000002
rosa_roxburghii	Rroxscaffold_5G00387340
rosa_rugosa	Rorug04G0377100
rosa_samantha	Rh4AG438600 Rh4BG447600 Rh4CG464700 Rh4DG446200
rosa_wichuraiana	Rw4G037510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	179
AccBSI	CCGCTC	1 cut(s)	120
AccI	GTMKAC	1 cut(s)	387
AccII	CGCG	1 cut(s)	204
AciI	CCGC	8 cut(s)	97, 103, 106, 118, 199, 202, 264, 273
AcoI	YGGCCR	1 cut(s)	270
AcsI	RAATTY	2 cut(s)	770, 868
AcuI	CTGAAG	1 cut(s)	713
AcyI	GRCGYC	1 cut(s)	11
AfeI	AGCGCT	1 cut(s)	1069
AfiI	CCNNNNNNNGG	6 cut(s)	205, 217, 398, 543, 544, 935
AflII	CTTAAG	1 cut(s)	701
AgsI	TTSAA	7 cut(s)	247, 325, 343, 439, 658, 766, 1078
AjnI	CCWGG	1 cut(s)	441
AluBI	AGCT	7 cut(s)	115, 487, 567, 677, 697, 863, 883
AluI	AGCT	7 cut(s)	115, 487, 567, 677, 697, 863, 883
Alw26I	GTCTC	2 cut(s)	22, 285
Ama87I	CYCGRG	1 cut(s)	287
Aor51HI	AGCGCT	1 cut(s)	1069
AoxI	GGCC	2 cut(s)	270, 781
ApeKI	GCWGC	2 cut(s)	223, 226
ApoI	RAATTY	2 cut(s)	770, 868
Asp700I	GAANNNNTTC	1 cut(s)	924
AspLEI	GCGC	2 cut(s)	198, 1070
AspS9I	GGNCC	1 cut(s)	208
AsuC2I	CCSGG	2 cut(s)	87, 392
AsuHPI	GGTGA	5 cut(s)	16, 121, 340, 400, 808
AvaI	CYCGRG	1 cut(s)	287
AvaII	GGWCC	1 cut(s)	208
BbsI	GAAGAC	2 cut(s)	303, 629
BbvCI	CCTCAGC	1 cut(s)	312
BbvI	GCAGC	2 cut(s)	235, 238
BccI	CCATC	8 cut(s)	170, 392, 626, 844, 887, 893, 958, 1010
BceAI	ACGGC	1 cut(s)	523
BciT130I	CCWGG	1 cut(s)	443
BcnI	CCSGG	2 cut(s)	87, 392
BcoDI	GTCTC	2 cut(s)	22, 285
BfaI	CTAG	1 cut(s)	864
BfoI	RGCGCY	2 cut(s)	199, 1071
BfrI	CTTAAG	1 cut(s)	701
BisI	GCNGC	6 cut(s)	106, 199, 202, 224, 227, 273
BlpI	GCTNAGC	1 cut(s)	879
BlsI	GCNGC	6 cut(s)	107, 200, 203, 225, 228, 274
Bme1390I	CCNGG	3 cut(s)	87, 392, 443
Bme18I	GGWCC	1 cut(s)	208
BmeT110I	CYCGRG	1 cut(s)	287
BmgT120I	GGNCC	1 cut(s)	208
BmrFI	CCNGG	3 cut(s)	87, 392, 443
BmrI	ACTGGG	1 cut(s)	534
BmsI	GCATC	3 cut(s)	472, 610, 623
BmuI	ACTGGG	1 cut(s)	534
BpiI	GAAGAC	2 cut(s)	303, 629
Bpu10I	CCTNAGC	1 cut(s)	312
Bpu1102I	GCTNAGC	1 cut(s)	879
BpuEI	CTTGAG	1 cut(s)	95
BpuMI	CCSGG	2 cut(s)	87, 392
BsaHI	GRCGYC	1 cut(s)	11
BsaJI	CCNNGG	2 cut(s)	411, 778
BsaXI	ACNNNNNCTCC	2 cut(s)	121, 151
Bsc4I	CCNNNNNNNGG	6 cut(s)	205, 217, 398, 543, 544, 935
Bse118I	RCCGGY	1 cut(s)	268
Bse1I	ACTGG	1 cut(s)	540
BseBI	CCWGG	1 cut(s)	443
BseDI	CCNNGG	2 cut(s)	411, 778
BseGI	GGATG	3 cut(s)	162, 614, 946
BseLI	CCNNNNNNNGG	6 cut(s)	205, 217, 398, 543, 544, 935
BseMII	CTCAG	7 cut(s)	33, 296, 303, 440, 461, 485, 893
BseNI	ACTGG	1 cut(s)	540
BseX3I	CGGCCG	1 cut(s)	270
BseXI	GCAGC	2 cut(s)	235, 238
BseYI	CCCAGC	3 cut(s)	673, 859, 1030
Bsh1236I	CGCG	1 cut(s)	204
Bsh1285I	CGRYCG	1 cut(s)	273
BshFI	GGCC	2 cut(s)	272, 783
BsiEI	CGRYCG	1 cut(s)	273
BsiHKCI	CYCGRG	1 cut(s)	287
BsiSI	CCGG	3 cut(s)	87, 269, 392
BslFI	GGGAC	1 cut(s)	749
BslI	CCNNNNNNNGG	6 cut(s)	205, 217, 398, 543, 544, 935
BsmAI	GTCTC	2 cut(s)	22, 285
BsmBI	CGTCTC	1 cut(s)	22
BsmFI	GGGAC	1 cut(s)	749
BsmI	GAATGC	1 cut(s)	559
BsnI	GGCC	2 cut(s)	272, 783
BsoBI	CYCGRG	1 cut(s)	287
Bsp143I	GATC	1 cut(s)	235
Bsp1720I	GCTNAGC	1 cut(s)	879
Bsp19I	CCATGG	1 cut(s)	778
BspACI	CCGC	8 cut(s)	97, 103, 106, 118, 199, 202, 264, 273
BspANI	GGCC	2 cut(s)	272, 783
BspCNI	CTCAG	7 cut(s)	32, 295, 304, 439, 460, 484, 892
BspFNI	CGCG	1 cut(s)	204
BspTI	CTTAAG	1 cut(s)	701
BsrBI	CCGCTC	1 cut(s)	120
BsrFI	RCCGGY	1 cut(s)	268
BsrI	ACTGG	1 cut(s)	540
BssAI	RCCGGY	1 cut(s)	268
BssECI	CCNNGG	2 cut(s)	411, 778
BssMI	GATC	1 cut(s)	235
BssNI	GRCGYC	1 cut(s)	11
BssT1I	CCWWGG	1 cut(s)	778
Bst2UI	CCWGG	1 cut(s)	443
Bst4CI	ACNGT	1 cut(s)	212
Bst6I	CTCTTC	1 cut(s)	1086
BstACI	GRCGYC	1 cut(s)	11
BstAFI	CTTAAG	1 cut(s)	701
BstAPI	GCANNNNNTGC	1 cut(s)	620
BstDEI	CTNAG	7 cut(s)	19, 282, 312, 426, 447, 471, 879
BstDSI	CCRYGG	1 cut(s)	778
BstF5I	GGATG	3 cut(s)	162, 614, 946
BstFNI	CGCG	1 cut(s)	204
BstH2I	RGCGCY	2 cut(s)	199, 1071
BstHHI	GCGC	2 cut(s)	198, 1070
BstKTI	GATC	1 cut(s)	238
BstMAI	GTCTC	2 cut(s)	22, 285
BstMBI	GATC	1 cut(s)	235
BstMCI	CGRYCG	1 cut(s)	273
BstMWI	GCNNNNNNNGC	7 cut(s)	96, 105, 248, 272, 573, 620, 1027
BstNI	CCWGG	1 cut(s)	443
BstSCI	CCNGG	3 cut(s)	85, 390, 441
BstUI	CGCG	1 cut(s)	204
BstV1I	GCAGC	2 cut(s)	235, 238
BstV2I	GAAGAC	2 cut(s)	303, 629
BstZI	CGGCCG	1 cut(s)	270
BsuRI	GGCC	2 cut(s)	272, 783
BtgI	CCRYGG	1 cut(s)	778
BtgZI	GCGATG	1 cut(s)	912
BtsCI	GGATG	3 cut(s)	162, 614, 946
BtsIMutI	CAGTG	1 cut(s)	434
CfoI	GCGC	2 cut(s)	198, 1070
Cfr10I	RCCGGY	1 cut(s)	268
Cfr13I	GGNCC	1 cut(s)	208
CseI	GACGC	1 cut(s)	317
CviAII	CATG	4 cut(s)	239, 304, 779, 1106
DdeI	CTNAG	7 cut(s)	19, 282, 312, 426, 447, 471, 879
DpnI	GATC	1 cut(s)	237
DpnII	GATC	1 cut(s)	235
DrdI	GACNNNNNNGTC	1 cut(s)	179
DseDI	GACNNNNNNGTC	1 cut(s)	179
EaeI	YGGCCR	1 cut(s)	270
EagI	CGGCCG	1 cut(s)	270
Eam1104I	CTCTTC	1 cut(s)	1086
EarI	CTCTTC	1 cut(s)	1086
EclXI	CGGCCG	1 cut(s)	270
Eco130I	CCWWGG	1 cut(s)	778
Eco47I	GGWCC	1 cut(s)	208
Eco47III	AGCGCT	1 cut(s)	1069
Eco52I	CGGCCG	1 cut(s)	270
Eco57I	CTGAAG	1 cut(s)	713
Eco88I	CYCGRG	1 cut(s)	287
EcoRI	GAATTC	1 cut(s)	770
EcoRII	CCWGG	1 cut(s)	441
EcoT14I	CCWWGG	1 cut(s)	778
EcoT22I	ATGCAT	1 cut(s)	616
ErhI	CCWWGG	1 cut(s)	778
Esp3I	CGTCTC	1 cut(s)	22
FaeI	CATG	4 cut(s)	242, 307, 782, 1109
FalI	AAGNNNNNCTT	2 cut(s)	746, 778
FaqI	GGGAC	1 cut(s)	749
FatI	CATG	4 cut(s)	238, 303, 778, 1105
FauNDI	CATATG	1 cut(s)	1054
FblI	GTMKAC	1 cut(s)	387
Fnu4HI	GCNGC	6 cut(s)	106, 199, 202, 224, 227, 273
FokI	GGATG	3 cut(s)	149, 601, 933
Fsp4HI	GCNGC	6 cut(s)	106, 199, 202, 224, 227, 273
FspBI	CTAG	1 cut(s)	864
GlaI	GCGC	2 cut(s)	197, 1069
GluI	GCNGC	6 cut(s)	106, 199, 202, 224, 227, 273
GsaI	CCCAGC	3 cut(s)	677, 863, 1034
HaeII	RGCGCY	2 cut(s)	199, 1071
HaeIII	GGCC	2 cut(s)	272, 783
HapII	CCGG	3 cut(s)	87, 269, 392
HgaI	GACGC	1 cut(s)	317
HhaI	GCGC	2 cut(s)	198, 1070
Hin1I	GRCGYC	1 cut(s)	11
Hin1II	CATG	4 cut(s)	242, 307, 782, 1109
Hin6I	GCGC	2 cut(s)	196, 1068
HinP1I	GCGC	2 cut(s)	196, 1068
HincII	GTYRAC	2 cut(s)	388, 423
HindII	GTYRAC	2 cut(s)	388, 423
HinfI	GANTC	4 cut(s)	260, 278, 402, 424
HpaII	CCGG	3 cut(s)	87, 269, 392
HphI	GGTGA	5 cut(s)	16, 121, 340, 400, 808
Hpy166II	GTNNAC	2 cut(s)	388, 423
Hpy188I	TCNGA	7 cut(s)	450, 474, 552, 619, 841, 913, 955
Hpy188III	TCNNGA	1 cut(s)	491
Hpy8I	GTNNAC	2 cut(s)	388, 423
Hpy99I	CGWCG	1 cut(s)	185
HpyAV	CCTTC	2 cut(s)	178, 253
HpyCH4III	ACNGT	1 cut(s)	212
HpyCH4IV	ACGT	1 cut(s)	345
HpyCH4V	TGCA	5 cut(s)	44, 463, 557, 614, 968
HpyF10VI	GCNNNNNNNGC	7 cut(s)	96, 105, 248, 272, 573, 620, 1027
HpyF3I	CTNAG	7 cut(s)	19, 282, 312, 426, 447, 471, 879
HpySE526I	ACGT	1 cut(s)	345
Hsp92I	GRCGYC	1 cut(s)	11
Hsp92II	CATG	4 cut(s)	242, 307, 782, 1109
HspAI	GCGC	2 cut(s)	196, 1068
Kzo9I	GATC	1 cut(s)	235
LmnI	GCTCC	6 cut(s)	120, 125, 142, 220, 484, 572
Lsp1109I	GCAGC	2 cut(s)	235, 238
LweI	GCATC	3 cut(s)	472, 610, 623
MaeI	CTAG	1 cut(s)	864
MaeII	ACGT	1 cut(s)	345
MaeIII	GTNAC	3 cut(s)	346, 406, 503
MalI	GATC	1 cut(s)	237
MbiI	CCGCTC	1 cut(s)	120
MboI	GATC	1 cut(s)	235
MboII	GAAGA	5 cut(s)	308, 629, 766, 824, 1103
MfeI	CAATTG	1 cut(s)	39
MluCI	AATT	4 cut(s)	39, 770, 823, 868
MlyI	GAGTC	3 cut(s)	254, 287, 418
MmeI	TCCRAC	1 cut(s)	147
Mph1103I	ATGCAT	1 cut(s)	616
MroXI	GAANNNNTTC	1 cut(s)	924
MseI	TTAA	5 cut(s)	609, 642, 702, 759, 1120
MslI	CAYNNNNRTG	2 cut(s)	609, 963
MspA1I	CMGCKG	1 cut(s)	677
MspCI	CTTAAG	1 cut(s)	701
MspI	CCGG	3 cut(s)	87, 269, 392
MspR9I	CCNGG	3 cut(s)	87, 392, 443
MunI	CAATTG	1 cut(s)	39
Mva1269I	GAATGC	1 cut(s)	559
MvaI	CCWGG	1 cut(s)	443
MvnI	CGCG	1 cut(s)	204
MwoI	GCNNNNNNNGC	7 cut(s)	96, 105, 248, 272, 573, 620, 1027
NciI	CCSGG	2 cut(s)	87, 392
NcoI	CCATGG	1 cut(s)	778
NdeI	CATATG	1 cut(s)	1054
NdeII	GATC	1 cut(s)	235
NlaIII	CATG	4 cut(s)	242, 307, 782, 1109
NmeAIII	GCCGAG	2 cut(s)	301, 550
NmuCI	GTSAC	3 cut(s)	346, 406, 503
NsiI	ATGCAT	1 cut(s)	616
PcsI	WCGNNNNNNNCGW	2 cut(s)	177, 255
PctI	GAATGC	1 cut(s)	559
PdmI	GAANNNNTTC	1 cut(s)	924
PfeI	GAWTC	1 cut(s)	402
PkrI	GCNGC	6 cut(s)	107, 200, 203, 225, 228, 274
PleI	GAGTC	3 cut(s)	254, 286, 418
PpsI	GAGTC	3 cut(s)	254, 286, 418
Psp6I	CCWGG	1 cut(s)	441
PspFI	CCCAGC	3 cut(s)	673, 859, 1030
PspGI	CCWGG	1 cut(s)	441
PspPI	GGNCC	1 cut(s)	208
PvuII	CAGCTG	1 cut(s)	677
RseI	CAYNNNNRTG	2 cut(s)	609, 963
SalI	GTCGAC	1 cut(s)	386
SaqAI	TTAA	5 cut(s)	609, 642, 702, 759, 1120
SatI	GCNGC	6 cut(s)	106, 199, 202, 224, 227, 273
Sau3AI	GATC	1 cut(s)	235
Sau96I	GGNCC	1 cut(s)	208
SchI	GAGTC	3 cut(s)	254, 287, 418
ScrFI	CCNGG	3 cut(s)	87, 392, 443
SfaNI	GCATC	3 cut(s)	472, 610, 623
SinI	GGWCC	1 cut(s)	208
SmiMI	CAYNNNNRTG	2 cut(s)	609, 963
SmlI	CTYRAG	2 cut(s)	110, 701
SmoI	CTYRAG	2 cut(s)	110, 701
Sse9I	AATT	4 cut(s)	39, 770, 823, 868
SsiI	CCGC	8 cut(s)	97, 103, 106, 118, 199, 202, 264, 273
SspMI	CTAG	1 cut(s)	864
StyD4I	CCNGG	3 cut(s)	85, 390, 441
StyI	CCWWGG	1 cut(s)	778
TaaI	ACNGT	1 cut(s)	212
TaiI	ACGT	1 cut(s)	348
TaqI	TCGA	4 cut(s)	171, 234, 387, 466
TasI	AATT	4 cut(s)	39, 770, 823, 868
TauI	GCSGC	4 cut(s)	108, 201, 204, 275
TfiI	GAWTC	1 cut(s)	402
Tru1I	TTAA	5 cut(s)	609, 642, 702, 759, 1120
Tru9I	TTAA	5 cut(s)	609, 642, 702, 759, 1120
TscAI	CASTG	1 cut(s)	434
TseFI	GTSAC	3 cut(s)	346, 406, 503
TseI	GCWGC	2 cut(s)	223, 226
Tsp45I	GTSAC	3 cut(s)	346, 406, 503
TspDTI	ATGAA	3 cut(s)	292, 765, 825
TspGWI	ACGGA	2 cut(s)	4, 1106
TspRI	CASTG	1 cut(s)	434
Vha464I	CTTAAG	1 cut(s)	701
VpaK11BI	GGWCC	1 cut(s)	208
XapI	RAATTY	2 cut(s)	770, 868
XmiI	GTMKAC	1 cut(s)	387
XmnI	GAANNNNTTC	1 cut(s)	924
XspI	CTAG	1 cut(s)	864
Zsp2I	ATGCAT	1 cut(s)	616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.