RchiOBHm_Chr4g0447021

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011504
Physical Location & Seq
Forward (+)
66929546 .. 66933055
3510 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41450

Sequence Viewer

Length: 1125 bp
ATGGGGAGGGCGTCGGTATTGATATACATCACCATAGCTCTCCTGGTGTTGCTTCTCATCTCCTGGTCCCCCAAGAAACCCGCCAACCACCGCCACCGCCGCCTCAAGCTCCGCTCCAACTTCACTTTCGCTCCTCCCCTCCACCATTCTCACAATCCCATCCCCTTCGACCCTCTCGTCGCCGACATCGAGCGCCGCCGCGAGGACCGCAAGTGGGAGAAGGAGCACCTCGACCTCGACCTCGATTTGGATCACCACGACTCCGCCCCCGCCGCCGAGTCCCAGCCCGAGTGGGAGGATTTCATGGACGCCGAGGACTACCTGAATGACGAGGACAAGTTCAATGTCACCAACAGGCTGGTCTTGTTGTTCCCCAAGGTCGATGTGGACCCCGTCGATGGCTTTATCACCGAGCACGAGCTGACTCTCTGGAACCTGAACCAGGCTCAGAGGGAAGTTTTGCATAGGACTCAGAGGGATATGGAGCTTCATGATAAGAATCGTGACGGCTTTGTTTCCTTCCAAGAGTACAAACCCCCTAGCTGGGTTCAGAATGCAGATAACAACTCATTTGGCTATGATATGGGCTGGTGGAAAGAAGAGCATTTTAATGCATCAGATGCAGATGGAGATGGTCTTCTCAATCTAACTGAGTTCAATGACTTCCTGCACCCGGCTGACAGCAAAAACCCAAAGCTACTTCAGTGGTTGTGCAAGGAGGAAGTAAGAGAGAGGGATACAGACAAAGATGGAAAGATTAACTTCAAAGAATTTTTCCATGGCCTCTTTGATTTGGTGAGGAACTATGATGAAGAAGATCACAATTCCACACATCAGTCTGATGATTTGATGGAGGCCCCGGCTAAAACTTTGTTTGCACAGCTTGACAAAGATGGTGACGGATACTTGTCAGATGTTGAGTTGCTTCCCATTATTGGAAAAATACATCCATCAGAACATTACTATGCGAAACAACAAGCAGATTACATCATATCACAGGCAGATACAAATAAAGATGGGGGCTTGTCACTGAGCGAGATGATTGAGAACCCATATGTATTTTACAGCGCCATTTTCAATGATGATGATGAAGAGGACTATGGGTTCCATGACGAGTTCCGTTAA

Protein Analysis

374

Amino Acids

43.8

Weight (kDa)

4.69

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 201 - 260 3e-06 EF-hand domain pair
EF-hand_7 PF13499 288 - 349 2e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011504)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08580 AT5G08580
fragaria_vesca FvH4_4g37230
malus_domestica MD00G1127000.v1.1 MD13G1002500.v1.1
prunus_persica Prupe.1G349900_v2.0.a1
pyrus_communis pycom13g00210 pycom16g00130
rosa_chinensis RchiOBHm_Chr4g0447021
rosa_laevigata RLG00000005612
rosa_multiflora Rmu_co8164088.1_g000001 Rmu_sc0001753.1_g000001 Rmu_sc0019356.1_g000002
rosa_roxburghii Rroxscaffold_5G00387340
rosa_rugosa Rorug04G0377100
rosa_samantha Rh4AG438600 Rh4BG447600 Rh4CG464700 Rh4DG446200
rosa_wichuraiana Rw4G037510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 1 cut(s) 201
AclWI GGATC 1 cut(s) 258
AcsI RAATTY 1 cut(s) 770
AcuI CTGAAG 1 cut(s) 686
AcyI GRCGYC 2 cut(s) 11, 309
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 9 cut(s) 202, 214, 247, 398, 442, 543, 544, 673, 935
AgsI TTSAA 4 cut(s) 343, 658, 766, 1078
AjnI CCWGG 3 cut(s) 42, 62, 441
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AloI GAACNNNNNNTCC 2 cut(s) 1088, 1120
AluBI AGCT 7 cut(s) 38, 109, 421, 487, 543, 697, 883
AluI AGCT 7 cut(s) 38, 109, 421, 487, 543, 697, 883
Alw21I GWGCWC 2 cut(s) 228, 417
AlwI GGATC 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 2 cut(s) 781, 855
ApoI RAATTY 1 cut(s) 770
AspLEI GCGC 2 cut(s) 195, 1070
AspS9I GGNCC 4 cut(s) 66, 205, 388, 856
AsuC2I CCSGG 2 cut(s) 674, 860
AsuHPI GGTGA 6 cut(s) 22, 245, 340, 400, 808, 908
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 3 cut(s) 66, 205, 388
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 629
Bbv12I GWGCWC 2 cut(s) 228, 417
BccI CCATC 9 cut(s) 167, 392, 620, 626, 743, 844, 887, 958, 1010
BceAI ACGGC 1 cut(s) 523
BciT130I CCWGG 3 cut(s) 44, 64, 443
BciVI GTATCC 2 cut(s) 730, 896
BcnI CCSGG 2 cut(s) 674, 860
BfaI CTAG 1 cut(s) 540
BfoI RGCGCY 2 cut(s) 196, 1071
BfuI GTATCC 2 cut(s) 730, 896
BisI GCNGC 4 cut(s) 100, 196, 199, 273
BlsI GCNGC 4 cut(s) 101, 197, 200, 274
Bme1390I CCNGG 5 cut(s) 44, 64, 443, 674, 860
Bme18I GGWCC 3 cut(s) 66, 205, 388
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 4 cut(s) 66, 205, 388, 856
BmiI GGNNCC 5 cut(s) 68, 390, 434, 858, 1106
BmrFI CCNGG 5 cut(s) 44, 64, 443, 674, 860
BmsI GCATC 2 cut(s) 610, 623
BpiI GAAGAC 1 cut(s) 629
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 2 cut(s) 674, 860
BsaBI GATNNNNATC 3 cut(s) 26, 249, 498
BsaHI GRCGYC 2 cut(s) 11, 309
BsaJI CCNNGG 4 cut(s) 312, 375, 778, 858
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 245, 275
Bsc4I CCNNNNNNNGG 9 cut(s) 202, 214, 247, 398, 442, 543, 544, 673, 935
Bse8I GATNNNNATC 3 cut(s) 26, 249, 498
BseBI CCWGG 3 cut(s) 44, 64, 443
BseDI CCNNGG 4 cut(s) 312, 375, 778, 858
BseGI GGATG 2 cut(s) 159, 946
BseJI GATNNNNATC 3 cut(s) 26, 249, 498
BseLI CCNNNNNNNGG 9 cut(s) 202, 214, 247, 398, 442, 543, 544, 673, 935
BseMII CTCAG 4 cut(s) 461, 485, 642, 1022
BseRI GAGGAG 1 cut(s) 123
BseYI CCCAGC 2 cut(s) 282, 543
BsgI GTGCAG 1 cut(s) 653
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 2 cut(s) 783, 857
BsiHKAI GWGCWC 2 cut(s) 228, 417
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 2 cut(s) 674, 860
BslFI GGGAC 2 cut(s) 52, 265
BslI CCNNNNNNNGG 9 cut(s) 202, 214, 247, 398, 442, 543, 544, 673, 935
BsmFI GGGAC 2 cut(s) 52, 265
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 2 cut(s) 783, 857
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 228, 417
Bsp143I GATC 2 cut(s) 250, 817
Bsp19I CCATGG 1 cut(s) 778
BspANI GGCC 2 cut(s) 783, 857
BspCNI CTCAG 4 cut(s) 460, 484, 643, 1023
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 5 cut(s) 68, 390, 434, 858, 1106
BspPI GGATC 1 cut(s) 258
BspQI GCTCTTC 1 cut(s) 594
BsrBI CCGCTC 1 cut(s) 114
BssECI CCNNGG 4 cut(s) 312, 375, 778, 858
BssMI GATC 2 cut(s) 250, 817
BssNI GRCGYC 2 cut(s) 11, 309
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 2 cut(s) 375, 778
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 44, 64, 443
Bst6I CTCTTC 2 cut(s) 594, 1086
BstACI GRCGYC 2 cut(s) 11, 309
BstAPI GCANNNNNTGC 1 cut(s) 620
BstDEI CTNAG 4 cut(s) 447, 471, 651, 1031
BstDSI CCRYGG 1 cut(s) 778
BstENI CCTNNNNNAGG 1 cut(s) 440
BstF5I GGATG 2 cut(s) 159, 946
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 2 cut(s) 196, 1071
BstHHI GCGC 2 cut(s) 195, 1070
BstKTI GATC 2 cut(s) 253, 820
BstMBI GATC 2 cut(s) 250, 817
BstMWI GCNNNNNNNGC 4 cut(s) 99, 207, 272, 620
BstNI CCWGG 3 cut(s) 44, 64, 443
BstSCI CCNGG 5 cut(s) 42, 62, 441, 672, 858
BstUI CGCG 1 cut(s) 201
BstV2I GAAGAC 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 358
BsuI GTATCC 2 cut(s) 730, 896
BsuRI GGCC 2 cut(s) 783, 857
BtgI CCRYGG 1 cut(s) 778
BtsCI GGATG 2 cut(s) 159, 946
BtsIMutI CAGTG 2 cut(s) 710, 1028
CciI TCATGA 1 cut(s) 490
CfoI GCGC 2 cut(s) 195, 1070
Cfr13I GGNCC 4 cut(s) 66, 205, 388, 856
CseI GACGC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 529
CviAII CATG 4 cut(s) 304, 491, 779, 1109
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 4 cut(s) 447, 471, 651, 1031
DpnI GATC 2 cut(s) 252, 819
DpnII GATC 2 cut(s) 250, 817
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
Eam1104I CTCTTC 2 cut(s) 594, 1086
EarI CTCTTC 2 cut(s) 594, 1086
EciI GGCGGA 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 375, 778
Eco47I GGWCC 3 cut(s) 66, 205, 388
Eco57I CTGAAG 1 cut(s) 686
Eco88I CYCGRG 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 440
EcoO109I RGGNCCY 1 cut(s) 856
EcoRII CCWGG 3 cut(s) 42, 62, 441
EcoT14I CCWWGG 2 cut(s) 375, 778
EcoT22I ATGCAT 1 cut(s) 616
ErhI CCWWGG 2 cut(s) 375, 778
FaeI CATG 4 cut(s) 307, 494, 782, 1112
FalI AAGNNNNNCTT 2 cut(s) 746, 778
FaqI GGGAC 2 cut(s) 52, 265
FatI CATG 4 cut(s) 303, 490, 778, 1108
FauI CCCGC 2 cut(s) 88, 277
FauNDI CATATG 1 cut(s) 1054
Fnu4HI GCNGC 4 cut(s) 100, 196, 199, 273
FokI GGATG 2 cut(s) 146, 933
Fsp4HI GCNGC 4 cut(s) 100, 196, 199, 273
FspBI CTAG 1 cut(s) 540
GlaI GCGC 2 cut(s) 194, 1069
GluI GCNGC 4 cut(s) 100, 196, 199, 273
GsaI CCCAGC 2 cut(s) 286, 547
HaeII RGCGCY 2 cut(s) 196, 1071
HaeIII GGCC 2 cut(s) 783, 857
HapII CCGG 2 cut(s) 674, 860
HgaI GACGC 1 cut(s) 317
HhaI GCGC 2 cut(s) 195, 1070
Hin1I GRCGYC 2 cut(s) 11, 309
Hin1II CATG 4 cut(s) 307, 494, 782, 1112
Hin6I GCGC 2 cut(s) 193, 1068
HinP1I GCGC 2 cut(s) 193, 1068
HinfI GANTC 5 cut(s) 260, 278, 424, 469, 499
HpaII CCGG 2 cut(s) 674, 860
HphI GGTGA 6 cut(s) 22, 245, 340, 400, 808, 908
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 7 cut(s) 450, 474, 552, 619, 841, 913, 955
Hpy188III TCNNGA 3 cut(s) 430, 491, 503
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 3 cut(s) 16, 182, 398
HpyAV CCTTC 3 cut(s) 175, 214, 529
HpyCH4V TGCA 7 cut(s) 463, 557, 614, 623, 670, 714, 878
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 207, 272, 620
HpyF3I CTNAG 4 cut(s) 447, 471, 651, 1031
Hsp92I GRCGYC 2 cut(s) 11, 309
Hsp92II CATG 4 cut(s) 307, 494, 782, 1112
HspAI GCGC 2 cut(s) 193, 1068
Kzo9I GATC 2 cut(s) 250, 817
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 5 cut(s) 114, 119, 136, 223, 484
LweI GCATC 2 cut(s) 610, 623
MaeI CTAG 1 cut(s) 540
MaeIII GTNAC 4 cut(s) 346, 503, 896, 1026
MalI GATC 2 cut(s) 252, 819
MbiI CCGCTC 1 cut(s) 114
MboI GATC 2 cut(s) 250, 817
MboII GAAGA 5 cut(s) 611, 629, 824, 827, 1103
MhlI GDGCHC 2 cut(s) 228, 417
MluCI AATT 2 cut(s) 770, 823
MlyI GAGTC 4 cut(s) 254, 287, 418, 463
MmeI TCCRAC 1 cut(s) 141
Mph1103I ATGCAT 1 cut(s) 616
MseI TTAA 3 cut(s) 609, 759, 1123
MslI CAYNNNNRTG 2 cut(s) 609, 963
MspI CCGG 2 cut(s) 674, 860
MspR9I CCNGG 5 cut(s) 44, 64, 443, 674, 860
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 3 cut(s) 44, 64, 443
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 99, 207, 272, 620
NciI CCSGG 2 cut(s) 674, 860
NcoI CCATGG 1 cut(s) 778
NdeI CATATG 1 cut(s) 1054
NdeII GATC 2 cut(s) 250, 817
NlaIII CATG 4 cut(s) 307, 494, 782, 1112
NlaIV GGNNCC 5 cut(s) 68, 390, 434, 858, 1106
NmeAIII GCCGAG 2 cut(s) 301, 337
NmuCI GTSAC 4 cut(s) 346, 503, 896, 1026
NsiI ATGCAT 1 cut(s) 616
PagI TCATGA 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 594
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 186
PctI GAATGC 1 cut(s) 559
PfeI GAWTC 1 cut(s) 499
PflFI GACNNNGTC 1 cut(s) 392
PkrI GCNGC 4 cut(s) 101, 197, 200, 274
PleI GAGTC 4 cut(s) 254, 286, 418, 463
PpsI GAGTC 4 cut(s) 254, 286, 418, 463
Psp6I CCWGG 3 cut(s) 42, 62, 441
PspFI CCCAGC 2 cut(s) 282, 543
PspGI CCWGG 3 cut(s) 42, 62, 441
PspN4I GGNNCC 5 cut(s) 68, 390, 434, 858, 1106
PspPI GGNCC 4 cut(s) 66, 205, 388, 856
PsyI GACNNNGTC 1 cut(s) 392
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
RseI CAYNNNNRTG 2 cut(s) 609, 963
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 3 cut(s) 609, 759, 1123
SatI GCNGC 4 cut(s) 100, 196, 199, 273
Sau3AI GATC 2 cut(s) 250, 817
Sau96I GGNCC 4 cut(s) 66, 205, 388, 856
SchI GAGTC 4 cut(s) 254, 287, 418, 463
ScrFI CCNGG 5 cut(s) 44, 64, 443, 674, 860
SduI GDGCHC 2 cut(s) 228, 417
SfaNI GCATC 2 cut(s) 610, 623
SinI GGWCC 3 cut(s) 66, 205, 388
SmiMI CAYNNNNRTG 2 cut(s) 609, 963
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 2 cut(s) 770, 823
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 5 cut(s) 42, 62, 441, 672, 858
StyI CCWWGG 2 cut(s) 375, 778
TaqI TCGA 7 cut(s) 168, 189, 231, 237, 243, 381, 396
TasI AATT 2 cut(s) 770, 823
TatI WGTACW 1 cut(s) 528
TauI GCSGC 4 cut(s) 102, 198, 201, 275
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 3 cut(s) 609, 759, 1123
Tru9I TTAA 3 cut(s) 609, 759, 1123
TscAI CASTG 2 cut(s) 710, 1035
TseFI GTSAC 4 cut(s) 346, 503, 896, 1026
Tsp45I GTSAC 4 cut(s) 346, 503, 896, 1026
TspDTI ATGAA 4 cut(s) 292, 479, 825, 1104
TspGWI ACGGA 2 cut(s) 915, 1109
TspRI CASTG 2 cut(s) 710, 1035
Tth111I GACNNNGTC 1 cut(s) 392
VpaK11BI GGWCC 3 cut(s) 66, 205, 388
XagI CCTNNNNNAGG 1 cut(s) 440
XapI RAATTY 1 cut(s) 770
XcmI CCANNNNNNNNNTGG 2 cut(s) 40, 382
XspI CTAG 1 cut(s) 540
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.