Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011504

Physical Location & Seq

Genomic Coordinates Reverse (-)

149605 .. 153342

Gene Structure 3738 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1002500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1434 bp

ATGGTGAGGCCGTCCGTCTCAGTAATCATATACTCAGCAATTGCAGTGCTTGTCCTCTTGCTCATTTCCCACTCCCCCAAGAAACCCGGCAACCACCGCCACCGCCGCCTCAAGCTCCGCTCCAACTTCACCTTCTCTCCTCCCCTCCACCACCATAACCCCATCCCCTTCGACCCTCTCGTTGCCGACATTGAGCGCCGCCGCGAGGACCGAAAGTGGGAGCAGCAGCACTTCGATCATGCCTTCAACGCCTCCCACCACTCCGCCCCCGCCGCCGAATCTCAGCCCGAGTGGGAAGACTTCATGGACGCTGAGGATTACTTGAACGACGACGAGAGGTTCAACGTCACCAGCAGGTTGCTCTTGTTGTTTCCTAAGGTTGATGTCGACCCGGCTGATGGATTCGTCACCGAGCACGAGTTGACTCAGTGGAACTTGAACCAGGCCCAGAGGGAAGTCTTGCATCGGACTGAGAGGGATATGGAGCTTCACGACAAGAATCTTGACGGCTTTGTTTCCTTTGCTGAGTATCAGCCCCCGAGTTGGGCTCAGAATGCAGATAACAGTTCTTTTGGCTATGATATGGGTTGGTGGAAAGAGGAGCATTTTAATGCATCAGATGCAAATGGAGATGGTCTTCTTAATTTAACCGAGTTCAACGACTTTCTACACCCAGCTGACAGCAAAAACCCAAAGCTACTTACGTGGTTGTGCAGGGAGGAAGTAAGGGAAAGGGACACCGACAAAGATGAAAAAATTAACTTCAAAGAATTCTTCCATGGCCTCTTTGATTTGGTGAGGAACTATGATGAAGAAGATCACAATTCCACACATCAGTCTGATGATTCGATGGAGTCCCCAGCTAGAAATTTGTTCGCTCAGCTTGACAAAGATGGTGACGGTTATTTGTCGGATGAGGAGATACTTCCTATTATTGGAAAGATACATCCATCAGAACATTACTATGCAAAACAACAAGCAGATTACATTATATCACAGGCTGATACGGATAAAGACGGGCGACTAACGCTGGGAGAGATGATAGAGAACCCATATGTATTTTACAGCGCTATTTTCAGTGACGACGAGGAAGAGGACTATGGGTACCATGATGAGACGCGGAAGAGTTCAAAATCTTTGTTGTTGGTTTTGTTTTTGTTTGTTGATATGGAACTAGATTATCAGATTATGAGAGAGAGTGATTCAACCTCAAGGGGGATTGGTGCCCCATGGCTGGCTCGGTTGGTTATCTGGAGCCATTTGGAAGAAGGATTTATTTATTTGATAGAGCTGCTTGACAATCGTCCTACTCGCTGTAAACATAAATGTAATAGGCAGACTGGTGAAGGAAGAAGAACATTTTTATCATTGTTATTTAAGATTGTTTGGCAGTCAAGTCGAGTTTTACCGTGTCTTCGGCAAGTTACAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005794 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0019538 GO:0031974 GO:0036211 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0070013 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

478

Amino Acids

55.76

Weight (kDa)

5.04

Isoelectric Point (pI)

45.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARC_Ca_bdg	PF10591	236 - 347	8.1e-06	Secreted protein acidic and rich in cysteine Ca binding region
EF-hand_7	PF13499	288 - 349	4.8e-09	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011504)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08580 AT5G08580
fragaria_vesca	FvH4_4g37230
malus_domestica	MD00G1127000.v1.1 MD13G1002500.v1.1
prunus_persica	Prupe.1G349900_v2.0.a1
pyrus_communis	pycom13g00210 pycom16g00130
rosa_chinensis	RchiOBHm_Chr4g0447021
rosa_laevigata	RLG00000005612
rosa_multiflora	Rmu_co8164088.1_g000001 Rmu_sc0001753.1_g000001 Rmu_sc0019356.1_g000002
rosa_roxburghii	Rroxscaffold_5G00387340
rosa_rugosa	Rorug04G0377100
rosa_samantha	Rh4AG438600 Rh4BG447600 Rh4CG464700 Rh4DG446200
rosa_wichuraiana	Rw4G037510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	345
Acc65I	GGTACC	1 cut(s)	1104
AccB1I	GGYRCC	2 cut(s)	1104, 1223
AccBSI	CCGCTC	1 cut(s)	120
AccI	GTMKAC	1 cut(s)	387
AccII	CGCG	2 cut(s)	204, 1120
AcsI	RAATTY	2 cut(s)	770, 868
AfaI	GTAC	1 cut(s)	1106
AfeI	AGCGCT	1 cut(s)	1069
AfiI	CCNNNNNNNGG	8 cut(s)	205, 217, 398, 543, 544, 935, 1215, 1234
AgsI	TTSAA	8 cut(s)	247, 325, 343, 439, 658, 766, 1131, 1206
AjnI	CCWGG	1 cut(s)	441
AjuI	GAANNNNNNNTTGG	2 cut(s)	116, 148
AluBI	AGCT	7 cut(s)	115, 487, 677, 697, 863, 883, 1291
AluI	AGCT	7 cut(s)	115, 487, 677, 697, 863, 883, 1291
Alw21I	GWGCWC	1 cut(s)	417
Alw26I	GTCTC	2 cut(s)	22, 1109
Ama87I	CYCGRG	2 cut(s)	287, 538
Aor51HI	AGCGCT	1 cut(s)	1069
AoxI	GGCC	3 cut(s)	8, 444, 781
ApeKI	GCWGC	3 cut(s)	223, 226, 1291
ApoI	RAATTY	2 cut(s)	770, 868
Asp718I	GGTACC	1 cut(s)	1104
AspLEI	GCGC	2 cut(s)	198, 1070
AspS9I	GGNCC	2 cut(s)	208, 445
AsuC2I	CCSGG	2 cut(s)	87, 392
AsuHPI	GGTGA	7 cut(s)	16, 121, 340, 400, 808, 908, 1355
AvaI	CYCGRG	2 cut(s)	287, 538
AvaII	GGWCC	1 cut(s)	208
AxyI	CCTNAGG	1 cut(s)	375
BaeGI	GKGCMC	1 cut(s)	1228
BanI	GGYRCC	2 cut(s)	1104, 1223
BanII	GRGCYC	1 cut(s)	550
BauI	CACGAG	1 cut(s)	416
BbsI	GAAGAC	3 cut(s)	303, 629, 1406
Bbv12I	GWGCWC	1 cut(s)	417
BbvCI	CCTCAGC	1 cut(s)	312
BbvI	GCAGC	3 cut(s)	235, 238, 1278
BccI	CCATC	6 cut(s)	170, 392, 626, 844, 887, 958
BceAI	ACGGC	1 cut(s)	523
BcgI	CGANNNNNNTGC	2 cut(s)	1379, 1413
BciT130I	CCWGG	1 cut(s)	443
BcnI	CCSGG	2 cut(s)	87, 392
BcoDI	GTCTC	2 cut(s)	22, 1109
BfaI	CTAG	2 cut(s)	864, 1175
BfoI	RGCGCY	2 cut(s)	199, 1071
BfuAI	ACCTGC	1 cut(s)	345
BisI	GCNGC	7 cut(s)	106, 199, 202, 224, 227, 273, 1292
BlpI	GCTNAGC	1 cut(s)	879
BlsI	GCNGC	7 cut(s)	107, 200, 203, 225, 228, 274, 1293
Bme1390I	CCNGG	3 cut(s)	87, 392, 443
Bme18I	GGWCC	1 cut(s)	208
BmeT110I	CYCGRG	2 cut(s)	287, 538
BmgT120I	GGNCC	2 cut(s)	208, 445
BmiI	GGNNCC	3 cut(s)	1106, 1225, 1256
BmrFI	CCNGG	3 cut(s)	87, 392, 443
BmsI	GCATC	3 cut(s)	472, 610, 623
BoxI	GACNNNNGTC	1 cut(s)	1302
BpiI	GAAGAC	3 cut(s)	303, 629, 1406
BplI	GAGNNNNNCTC	2 cut(s)	532, 564
BpmI	CTGGAG	1 cut(s)	1273
Bpu10I	CCTNAGC	1 cut(s)	312
Bpu1102I	GCTNAGC	1 cut(s)	879
BpuEI	CTTGAG	2 cut(s)	95, 1195
BpuMI	CCSGG	2 cut(s)	87, 392
BsaAI	YACGTR	1 cut(s)	705
BsaJI	CCNNGG	2 cut(s)	778, 1229
BsaXI	ACNNNNNCTCC	2 cut(s)	121, 151
Bsc4I	CCNNNNNNNGG	8 cut(s)	205, 217, 398, 543, 544, 935, 1215, 1234
Bse1I	ACTGG	1 cut(s)	1345
Bse21I	CCTNAGG	1 cut(s)	375
BseBI	CCWGG	1 cut(s)	443
BseDI	CCNNGG	2 cut(s)	778, 1229
BseGI	GGATG	3 cut(s)	162, 919, 946
BseLI	CCNNNNNNNGG	8 cut(s)	205, 217, 398, 543, 544, 935, 1215, 1234
BseMII	CTCAG	9 cut(s)	33, 48, 296, 303, 440, 462, 516, 563, 893
BseNI	ACTGG	1 cut(s)	1345
BseRI	GAGGAG	3 cut(s)	129, 614, 932
BseSI	GKGCMC	1 cut(s)	1228
BseXI	GCAGC	3 cut(s)	235, 238, 1278
BseYI	CCCAGC	3 cut(s)	673, 859, 1030
BsgI	GTGCAG	1 cut(s)	733
Bsh1236I	CGCG	2 cut(s)	204, 1120
BshFI	GGCC	3 cut(s)	10, 446, 783
BshNI	GGYRCC	2 cut(s)	1104, 1223
BsiHKAI	GWGCWC	1 cut(s)	417
BsiHKCI	CYCGRG	2 cut(s)	287, 538
BsiSI	CCGG	2 cut(s)	87, 392
BslFI	GGGAC	2 cut(s)	749, 841
BslI	CCNNNNNNNGG	8 cut(s)	205, 217, 398, 543, 544, 935, 1215, 1234
BsmAI	GTCTC	2 cut(s)	22, 1109
BsmBI	CGTCTC	2 cut(s)	22, 1109
BsmFI	GGGAC	2 cut(s)	749, 841
BsmI	GAATGC	1 cut(s)	559
BsnI	GGCC	3 cut(s)	10, 446, 783
BsoBI	CYCGRG	2 cut(s)	287, 538
Bsp1286I	GDGCHC	3 cut(s)	417, 550, 1228
Bsp143I	GATC	2 cut(s)	235, 817
Bsp1720I	GCTNAGC	1 cut(s)	879
Bsp19I	CCATGG	2 cut(s)	778, 1229
BspANI	GGCC	3 cut(s)	10, 446, 783
BspCNI	CTCAG	9 cut(s)	32, 47, 295, 304, 439, 463, 517, 562, 892
BspFNI	CGCG	2 cut(s)	204, 1120
BspLI	GGNNCC	3 cut(s)	1106, 1225, 1256
BspMI	ACCTGC	1 cut(s)	345
BspT107I	GGYRCC	2 cut(s)	1104, 1223
BsrBI	CCGCTC	1 cut(s)	120
BsrI	ACTGG	1 cut(s)	1345
BssECI	CCNNGG	2 cut(s)	778, 1229
BssMI	GATC	2 cut(s)	235, 817
BssSI	CACGAG	1 cut(s)	416
BssT1I	CCWWGG	2 cut(s)	778, 1229
Bst2BI	CACGAG	1 cut(s)	416
Bst2UI	CCWGG	1 cut(s)	443
Bst4CI	ACNGT	4 cut(s)	566, 902, 1410, 1429
Bst6I	CTCTTC	2 cut(s)	1086, 1118
BstAPI	GCANNNNNTGC	1 cut(s)	620
BstBAI	YACGTR	1 cut(s)	705
BstC8I	GCNNGC	1 cut(s)	1236
BstDSI	CCRYGG	2 cut(s)	778, 1229
BstF5I	GGATG	3 cut(s)	162, 919, 946
BstFNI	CGCG	2 cut(s)	204, 1120
BstH2I	RGCGCY	2 cut(s)	199, 1071
BstHHI	GCGC	2 cut(s)	198, 1070
BstKTI	GATC	2 cut(s)	238, 820
BstMAI	GTCTC	2 cut(s)	22, 1109
BstMBI	GATC	2 cut(s)	235, 817
BstMWI	GCNNNNNNNGC	7 cut(s)	96, 105, 248, 272, 554, 620, 1027
BstNI	CCWGG	1 cut(s)	443
BstPAI	GACNNNNGTC	1 cut(s)	1302
BstSCI	CCNGG	3 cut(s)	85, 390, 441
BstSLI	GKGCMC	1 cut(s)	1228
BstUI	CGCG	2 cut(s)	204, 1120
BstV1I	GCAGC	3 cut(s)	235, 238, 1278
BstV2I	GAAGAC	3 cut(s)	303, 629, 1406
Bsu36I	CCTNAGG	1 cut(s)	375
BsuRI	GGCC	3 cut(s)	10, 446, 783
BtgI	CCRYGG	2 cut(s)	778, 1229
BtsCI	GGATG	3 cut(s)	162, 919, 946
BtsI	GCAGTG	1 cut(s)	51
BtsIMutI	CAGTG	3 cut(s)	51, 434, 1084
BveI	ACCTGC	1 cut(s)	345
Cac8I	GCNNGC	1 cut(s)	1236
CfoI	GCGC	2 cut(s)	198, 1070
Cfr13I	GGNCC	2 cut(s)	208, 445
CseI	GACGC	2 cut(s)	317, 1126
Csp6I	GTAC	1 cut(s)	1105
CviAII	CATG	5 cut(s)	239, 304, 779, 1109, 1230
CviQI	GTAC	1 cut(s)	1105
DpnI	GATC	2 cut(s)	237, 819
DpnII	GATC	2 cut(s)	235, 817
Eam1104I	CTCTTC	2 cut(s)	1086, 1118
EarI	CTCTTC	2 cut(s)	1086, 1118
EciI	GGCGGA	1 cut(s)	253
Eco130I	CCWWGG	2 cut(s)	778, 1229
Eco24I	GRGCYC	1 cut(s)	550
Eco47I	GGWCC	1 cut(s)	208
Eco47III	AGCGCT	1 cut(s)	1069
Eco81I	CCTNAGG	1 cut(s)	375
Eco88I	CYCGRG	2 cut(s)	287, 538
EcoRI	GAATTC	1 cut(s)	770
EcoRII	CCWGG	1 cut(s)	441
EcoT14I	CCWWGG	2 cut(s)	778, 1229
EcoT22I	ATGCAT	1 cut(s)	616
EcoT38I	GRGCYC	1 cut(s)	550
ErhI	CCWWGG	2 cut(s)	778, 1229
Esp3I	CGTCTC	2 cut(s)	22, 1109
FaeI	CATG	5 cut(s)	242, 307, 782, 1112, 1233
FaqI	GGGAC	2 cut(s)	749, 841
FatI	CATG	5 cut(s)	238, 303, 778, 1108, 1229
FauI	CCCGC	1 cut(s)	277
FauNDI	CATATG	1 cut(s)	1054
FblI	GTMKAC	1 cut(s)	387
Fnu4HI	GCNGC	7 cut(s)	106, 199, 202, 224, 227, 273, 1292
FokI	GGATG	3 cut(s)	149, 926, 933
FriOI	GRGCYC	1 cut(s)	550
Fsp4HI	GCNGC	7 cut(s)	106, 199, 202, 224, 227, 273, 1292
FspBI	CTAG	2 cut(s)	864, 1175
GlaI	GCGC	2 cut(s)	197, 1069
GluI	GCNGC	7 cut(s)	106, 199, 202, 224, 227, 273, 1292
GsaI	CCCAGC	3 cut(s)	677, 863, 1034
GsuI	CTGGAG	1 cut(s)	1273
HaeII	RGCGCY	2 cut(s)	199, 1071
HaeIII	GGCC	3 cut(s)	10, 446, 783
HapII	CCGG	2 cut(s)	87, 392
HgaI	GACGC	2 cut(s)	317, 1126
HhaI	GCGC	2 cut(s)	198, 1070
Hin1II	CATG	5 cut(s)	242, 307, 782, 1112, 1233
Hin6I	GCGC	2 cut(s)	196, 1068
HinP1I	GCGC	2 cut(s)	196, 1068
HincII	GTYRAC	2 cut(s)	388, 423
HindII	GTYRAC	2 cut(s)	388, 423
HinfI	GANTC	7 cut(s)	278, 402, 424, 499, 845, 854, 1202
HpaII	CCGG	2 cut(s)	87, 392
HphI	GGTGA	7 cut(s)	16, 121, 340, 400, 808, 908, 1355
Hpy166II	GTNNAC	3 cut(s)	388, 423, 1319
Hpy188I	TCNGA	7 cut(s)	468, 552, 619, 841, 913, 955, 1185
Hpy188III	TCNNGA	3 cut(s)	491, 503, 1252
Hpy8I	GTNNAC	3 cut(s)	388, 423, 1319
Hpy99I	CGWCG	3 cut(s)	332, 335, 1088
HpyAV	CCTTC	5 cut(s)	142, 178, 253, 1262, 1340
HpyCH4III	ACNGT	4 cut(s)	566, 902, 1410, 1429
HpyCH4IV	ACGT	2 cut(s)	345, 704
HpyCH4V	TGCA	7 cut(s)	44, 463, 557, 614, 623, 714, 968
HpyF10VI	GCNNNNNNNGC	7 cut(s)	96, 105, 248, 272, 554, 620, 1027
HpySE526I	ACGT	2 cut(s)	345, 704
Hsp92II	CATG	5 cut(s)	242, 307, 782, 1112, 1233
HspAI	GCGC	2 cut(s)	196, 1068
KpnI	GGTACC	1 cut(s)	1108
Kzo9I	GATC	2 cut(s)	235, 817
LmnI	GCTCC	6 cut(s)	120, 125, 220, 484, 601, 1254
Lsp1109I	GCAGC	3 cut(s)	235, 238, 1278
LweI	GCATC	3 cut(s)	472, 610, 623
MaeI	CTAG	2 cut(s)	864, 1175
MaeII	ACGT	2 cut(s)	345, 704
MaeIII	GTNAC	5 cut(s)	346, 406, 896, 1079, 1423
MalI	GATC	2 cut(s)	237, 819
MbiI	CCGCTC	1 cut(s)	120
MboI	GATC	2 cut(s)	235, 817
MfeI	CAATTG	1 cut(s)	39
MhlI	GDGCHC	3 cut(s)	417, 550, 1228
MluCI	AATT	6 cut(s)	39, 643, 756, 770, 823, 868
MlyI	GAGTC	2 cut(s)	418, 863
MmeI	TCCRAC	2 cut(s)	147, 891
Mph1103I	ATGCAT	1 cut(s)	616
MseI	TTAA	6 cut(s)	609, 642, 647, 759, 1377, 1432
MslI	CAYNNNNRTG	2 cut(s)	609, 963
MspA1I	CMGCKG	1 cut(s)	677
MspI	CCGG	2 cut(s)	87, 392
MspR9I	CCNGG	3 cut(s)	87, 392, 443
MunI	CAATTG	1 cut(s)	39
Mva1269I	GAATGC	1 cut(s)	559
MvaI	CCWGG	1 cut(s)	443
MvnI	CGCG	2 cut(s)	204, 1120
MwoI	GCNNNNNNNGC	7 cut(s)	96, 105, 248, 272, 554, 620, 1027
NciI	CCSGG	2 cut(s)	87, 392
NcoI	CCATGG	2 cut(s)	778, 1229
NdeI	CATATG	1 cut(s)	1054
NdeII	GATC	2 cut(s)	235, 817
NlaIII	CATG	5 cut(s)	242, 307, 782, 1112, 1233
NlaIV	GGNNCC	3 cut(s)	1106, 1225, 1256
NmuCI	GTSAC	4 cut(s)	346, 406, 896, 1079
NsiI	ATGCAT	1 cut(s)	616
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PctI	GAATGC	1 cut(s)	559
PfeI	GAWTC	5 cut(s)	278, 402, 499, 845, 1202
PkrI	GCNGC	7 cut(s)	107, 200, 203, 225, 228, 274, 1293
PleI	GAGTC	2 cut(s)	418, 862
PpsI	GAGTC	2 cut(s)	418, 862
Ppu21I	YACGTR	1 cut(s)	705
PshAI	GACNNNNGTC	1 cut(s)	1302
Psp6I	CCWGG	1 cut(s)	441
PspFI	CCCAGC	3 cut(s)	673, 859, 1030
PspGI	CCWGG	1 cut(s)	441
PspN4I	GGNNCC	3 cut(s)	1106, 1225, 1256
PspPI	GGNCC	2 cut(s)	208, 445
PvuII	CAGCTG	1 cut(s)	677
RsaI	GTAC	1 cut(s)	1106
RsaNI	GTAC	1 cut(s)	1105
RseI	CAYNNNNRTG	2 cut(s)	609, 963
SalI	GTCGAC	1 cut(s)	386
SaqAI	TTAA	6 cut(s)	609, 642, 647, 759, 1377, 1432
SatI	GCNGC	7 cut(s)	106, 199, 202, 224, 227, 273, 1292
Sau3AI	GATC	2 cut(s)	235, 817
Sau96I	GGNCC	2 cut(s)	208, 445
SchI	GAGTC	2 cut(s)	418, 863
ScrFI	CCNGG	3 cut(s)	87, 392, 443
SduI	GDGCHC	3 cut(s)	417, 550, 1228
SfaNI	GCATC	3 cut(s)	472, 610, 623
SinI	GGWCC	1 cut(s)	208
SmiMI	CAYNNNNRTG	2 cut(s)	609, 963
SmlI	CTYRAG	2 cut(s)	110, 1210
SmoI	CTYRAG	2 cut(s)	110, 1210
Sse9I	AATT	6 cut(s)	39, 643, 756, 770, 823, 868
SspMI	CTAG	2 cut(s)	864, 1175
StyD4I	CCNGG	3 cut(s)	85, 390, 441
StyI	CCWWGG	2 cut(s)	778, 1229
TaaI	ACNGT	4 cut(s)	566, 902, 1410, 1429
TaiI	ACGT	2 cut(s)	348, 707
TaqI	TCGA	5 cut(s)	171, 234, 387, 848, 1399
TaqII	GACCGA	1 cut(s)	225
TasI	AATT	6 cut(s)	39, 643, 756, 770, 823, 868
TauI	GCSGC	4 cut(s)	108, 201, 204, 275
TfiI	GAWTC	5 cut(s)	278, 402, 499, 845, 1202
Tru1I	TTAA	6 cut(s)	609, 642, 647, 759, 1377, 1432
Tru9I	TTAA	6 cut(s)	609, 642, 647, 759, 1377, 1432
TscAI	CASTG	3 cut(s)	51, 434, 1084
TseFI	GTSAC	4 cut(s)	346, 406, 896, 1079
TseI	GCWGC	3 cut(s)	223, 226, 1291
Tsp45I	GTSAC	4 cut(s)	346, 406, 896, 1079
TspDTI	ATGAA	3 cut(s)	292, 765, 825
TspGWI	ACGGA	2 cut(s)	4, 1022
TspRI	CASTG	3 cut(s)	51, 434, 1084
VpaK11BI	GGWCC	1 cut(s)	208
XapI	RAATTY	2 cut(s)	770, 868
XmiI	GTMKAC	1 cut(s)	387
XspI	CTAG	2 cut(s)	864, 1175
Zsp2I	ATGCAT	1 cut(s)	616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.