Rh4AG438600

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011504
Physical Location & Seq
Forward (+)
73052883 .. 73056282
3400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG438600.1

Sequence Viewer

Length: 1125 bp
ATGGGGAGGGCGTCGGTATTGATATACATCACCATAGCTCTCCTGGTGTTGCTGCTCATCTCCTGGTCCCCCAAGAAACCCGCCAACCACCGCCACCGCCGCCTCAAGCTCCGCTCCAACTTCACTTTCGCTCCTCCCCTCCACCATTCTCACAATCCCATCCCCTTCGACCCTCTCGTCGCCGACATCGAGCGCCGCCGCGAGGACCGCAAGTGGGAGAAGGAGCACCTCGACCTCGACCTCGATTTGGATCACCACGACTCCGCCCCCGCCGCCGAGTCCCAGCCCGAGTGGGAGGATTTCATGGACGCCGAGGACTACCTGAATGACGAGGACAAGTTCAATGTCACCAACAGGCTGGTCTTGTTGTTCCCCAAGGTCGATGTGGACCCCGTCGATGGCTTTATCACCGAGCACGAGCTGACTCTCTGGAACCTGAACCAGGCTCAGAGGGAAGTTTTGCATAGGACTCAGAGGGATATGGAGCTTCATGATAAGAATCGTGACGGCTTTGTTTCCTTCCAAGAGTACAAACCCCCTAGCTGGGTTCAGAATGCAGATAACAACTCATTTGGCTATGATATGGGCTGGTGGAAAGAAGAGCATTTTAATGCATCAGATGCAGATGGAGATGGTCTTCTCAATCTAACTGAGTTCAATGACTTCCTGCACCCGGCTGACAGCAAAAACCCAAAGCTACTTCAGTGGCTGTGCAAGGAGGAAGTAAGAGAGAGGGATACAGACAAAGATGGAAAGATTAACTTCAAAGAATTTTTCCATGGCCTCTTTGATTTGGTGAGGAACTATGATGAAGAAGATCACAATTCCACACATCAGTCTGATGATTTGATGGAGGCCCCGGCTAAAACTTTGTTTGCACAGCTTGACAAAGATGGTGACGGATACTTGTCAGATGTTGAGTTGCTTCCCATTATTGGAAAAATACATCCATCAGAACATTACTATGCGAAACAACAAGCAGATTACATCATATCACAGGCAGATACAAATAAAGATGGGGGCTTGTCACTGAGCGAGATGATTGAGAACCCATATGTATTTTACAGCGCCATTTTCAATGATGATGATGAAGAGGACTATGGGTTCCATGACGAGTTCCGTTAA

Protein Analysis

374

Amino Acids

43.8

Weight (kDa)

4.69

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 201 - 260 3e-06 EF-hand domain pair
EF-hand_7 PF13499 288 - 349 2e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011504)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08580 AT5G08580
fragaria_vesca FvH4_4g37230
malus_domestica MD00G1127000.v1.1 MD13G1002500.v1.1
prunus_persica Prupe.1G349900_v2.0.a1
pyrus_communis pycom13g00210 pycom16g00130
rosa_chinensis RchiOBHm_Chr4g0447021
rosa_laevigata RLG00000005612
rosa_multiflora Rmu_co8164088.1_g000001 Rmu_sc0001753.1_g000001 Rmu_sc0019356.1_g000002
rosa_roxburghii Rroxscaffold_5G00387340
rosa_rugosa Rorug04G0377100
rosa_samantha Rh4AG438600 Rh4BG447600 Rh4CG464700 Rh4DG446200
rosa_wichuraiana Rw4G037510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 1 cut(s) 201
AclWI GGATC 1 cut(s) 258
AcsI RAATTY 1 cut(s) 770
AcuI CTGAAG 1 cut(s) 686
AcyI GRCGYC 2 cut(s) 11, 309
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 9 cut(s) 202, 214, 247, 398, 442, 543, 544, 673, 935
AgsI TTSAA 4 cut(s) 343, 658, 766, 1078
AjnI CCWGG 3 cut(s) 42, 62, 441
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AloI GAACNNNNNNTCC 2 cut(s) 1088, 1120
AluBI AGCT 7 cut(s) 38, 109, 421, 487, 543, 697, 883
AluI AGCT 7 cut(s) 38, 109, 421, 487, 543, 697, 883
Alw21I GWGCWC 2 cut(s) 228, 417
AlwI GGATC 1 cut(s) 258
AlwNI CAGNNNCTG 1 cut(s) 709
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 2 cut(s) 781, 855
ApeKI GCWGC 1 cut(s) 52
ApoI RAATTY 1 cut(s) 770
AspLEI GCGC 2 cut(s) 195, 1070
AspS9I GGNCC 4 cut(s) 66, 205, 388, 856
AsuC2I CCSGG 2 cut(s) 674, 860
AsuHPI GGTGA 6 cut(s) 22, 245, 340, 400, 808, 908
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 3 cut(s) 66, 205, 388
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 629
Bbv12I GWGCWC 2 cut(s) 228, 417
BbvI GCAGC 1 cut(s) 39
BccI CCATC 9 cut(s) 167, 392, 620, 626, 743, 844, 887, 958, 1010
BceAI ACGGC 1 cut(s) 523
BciT130I CCWGG 3 cut(s) 44, 64, 443
BciVI GTATCC 2 cut(s) 730, 896
BcnI CCSGG 2 cut(s) 674, 860
BfaI CTAG 1 cut(s) 540
BfoI RGCGCY 2 cut(s) 196, 1071
BfuI GTATCC 2 cut(s) 730, 896
BisI GCNGC 5 cut(s) 53, 100, 196, 199, 273
BlsI GCNGC 5 cut(s) 54, 101, 197, 200, 274
Bme1390I CCNGG 5 cut(s) 44, 64, 443, 674, 860
Bme18I GGWCC 3 cut(s) 66, 205, 388
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 4 cut(s) 66, 205, 388, 856
BmiI GGNNCC 5 cut(s) 68, 390, 434, 858, 1106
BmrFI CCNGG 5 cut(s) 44, 64, 443, 674, 860
BmsI GCATC 2 cut(s) 610, 623
BpiI GAAGAC 1 cut(s) 629
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 2 cut(s) 674, 860
BsaBI GATNNNNATC 3 cut(s) 26, 249, 498
BsaHI GRCGYC 2 cut(s) 11, 309
BsaJI CCNNGG 4 cut(s) 312, 375, 778, 858
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 245, 275
Bsc4I CCNNNNNNNGG 9 cut(s) 202, 214, 247, 398, 442, 543, 544, 673, 935
Bse8I GATNNNNATC 3 cut(s) 26, 249, 498
BseBI CCWGG 3 cut(s) 44, 64, 443
BseDI CCNNGG 4 cut(s) 312, 375, 778, 858
BseGI GGATG 2 cut(s) 159, 946
BseJI GATNNNNATC 3 cut(s) 26, 249, 498
BseLI CCNNNNNNNGG 9 cut(s) 202, 214, 247, 398, 442, 543, 544, 673, 935
BseMII CTCAG 4 cut(s) 461, 485, 642, 1022
BseRI GAGGAG 1 cut(s) 123
BseXI GCAGC 1 cut(s) 39
BseYI CCCAGC 2 cut(s) 282, 543
BsgI GTGCAG 1 cut(s) 653
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 2 cut(s) 783, 857
BsiHKAI GWGCWC 2 cut(s) 228, 417
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 2 cut(s) 674, 860
BslFI GGGAC 2 cut(s) 52, 265
BslI CCNNNNNNNGG 9 cut(s) 202, 214, 247, 398, 442, 543, 544, 673, 935
BsmFI GGGAC 2 cut(s) 52, 265
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 2 cut(s) 783, 857
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 228, 417
Bsp143I GATC 2 cut(s) 250, 817
Bsp19I CCATGG 1 cut(s) 778
BspANI GGCC 2 cut(s) 783, 857
BspCNI CTCAG 4 cut(s) 460, 484, 643, 1023
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 5 cut(s) 68, 390, 434, 858, 1106
BspPI GGATC 1 cut(s) 258
BspQI GCTCTTC 1 cut(s) 594
BsrBI CCGCTC 1 cut(s) 114
BssECI CCNNGG 4 cut(s) 312, 375, 778, 858
BssMI GATC 2 cut(s) 250, 817
BssNI GRCGYC 2 cut(s) 11, 309
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 2 cut(s) 375, 778
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 44, 64, 443
Bst6I CTCTTC 2 cut(s) 594, 1086
BstACI GRCGYC 2 cut(s) 11, 309
BstAPI GCANNNNNTGC 1 cut(s) 620
BstDEI CTNAG 4 cut(s) 447, 471, 651, 1031
BstDSI CCRYGG 1 cut(s) 778
BstENI CCTNNNNNAGG 1 cut(s) 440
BstF5I GGATG 2 cut(s) 159, 946
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 2 cut(s) 196, 1071
BstHHI GCGC 2 cut(s) 195, 1070
BstKTI GATC 2 cut(s) 253, 820
BstMBI GATC 2 cut(s) 250, 817
BstMWI GCNNNNNNNGC 4 cut(s) 99, 207, 272, 620
BstNI CCWGG 3 cut(s) 44, 64, 443
BstSCI CCNGG 5 cut(s) 42, 62, 441, 672, 858
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 39
BstV2I GAAGAC 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 358
BsuI GTATCC 2 cut(s) 730, 896
BsuRI GGCC 2 cut(s) 783, 857
BtgI CCRYGG 1 cut(s) 778
BtsCI GGATG 2 cut(s) 159, 946
BtsIMutI CAGTG 2 cut(s) 710, 1028
CaiI CAGNNNCTG 1 cut(s) 709
CciI TCATGA 1 cut(s) 490
CfoI GCGC 2 cut(s) 195, 1070
Cfr13I GGNCC 4 cut(s) 66, 205, 388, 856
CseI GACGC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 529
CviAII CATG 4 cut(s) 304, 491, 779, 1109
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 4 cut(s) 447, 471, 651, 1031
DpnI GATC 2 cut(s) 252, 819
DpnII GATC 2 cut(s) 250, 817
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
Eam1104I CTCTTC 2 cut(s) 594, 1086
EarI CTCTTC 2 cut(s) 594, 1086
EciI GGCGGA 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 375, 778
Eco47I GGWCC 3 cut(s) 66, 205, 388
Eco57I CTGAAG 1 cut(s) 686
Eco88I CYCGRG 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 440
EcoO109I RGGNCCY 1 cut(s) 856
EcoRII CCWGG 3 cut(s) 42, 62, 441
EcoT14I CCWWGG 2 cut(s) 375, 778
EcoT22I ATGCAT 1 cut(s) 616
ErhI CCWWGG 2 cut(s) 375, 778
FaeI CATG 4 cut(s) 307, 494, 782, 1112
FalI AAGNNNNNCTT 2 cut(s) 746, 778
FaqI GGGAC 2 cut(s) 52, 265
FatI CATG 4 cut(s) 303, 490, 778, 1108
FauI CCCGC 2 cut(s) 88, 277
FauNDI CATATG 1 cut(s) 1054
Fnu4HI GCNGC 5 cut(s) 53, 100, 196, 199, 273
FokI GGATG 2 cut(s) 146, 933
Fsp4HI GCNGC 5 cut(s) 53, 100, 196, 199, 273
FspBI CTAG 1 cut(s) 540
GlaI GCGC 2 cut(s) 194, 1069
GluI GCNGC 5 cut(s) 53, 100, 196, 199, 273
GsaI CCCAGC 2 cut(s) 286, 547
HaeII RGCGCY 2 cut(s) 196, 1071
HaeIII GGCC 2 cut(s) 783, 857
HapII CCGG 2 cut(s) 674, 860
HgaI GACGC 1 cut(s) 317
HhaI GCGC 2 cut(s) 195, 1070
Hin1I GRCGYC 2 cut(s) 11, 309
Hin1II CATG 4 cut(s) 307, 494, 782, 1112
Hin6I GCGC 2 cut(s) 193, 1068
HinP1I GCGC 2 cut(s) 193, 1068
HinfI GANTC 5 cut(s) 260, 278, 424, 469, 499
HpaII CCGG 2 cut(s) 674, 860
HphI GGTGA 6 cut(s) 22, 245, 340, 400, 808, 908
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 7 cut(s) 450, 474, 552, 619, 841, 913, 955
Hpy188III TCNNGA 3 cut(s) 430, 491, 503
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 3 cut(s) 16, 182, 398
HpyAV CCTTC 3 cut(s) 175, 214, 529
HpyCH4V TGCA 7 cut(s) 463, 557, 614, 623, 670, 714, 878
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 207, 272, 620
HpyF3I CTNAG 4 cut(s) 447, 471, 651, 1031
Hsp92I GRCGYC 2 cut(s) 11, 309
Hsp92II CATG 4 cut(s) 307, 494, 782, 1112
HspAI GCGC 2 cut(s) 193, 1068
Kzo9I GATC 2 cut(s) 250, 817
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 5 cut(s) 114, 119, 136, 223, 484
Lsp1109I GCAGC 1 cut(s) 39
LweI GCATC 2 cut(s) 610, 623
MaeI CTAG 1 cut(s) 540
MaeIII GTNAC 4 cut(s) 346, 503, 896, 1026
MalI GATC 2 cut(s) 252, 819
MbiI CCGCTC 1 cut(s) 114
MboI GATC 2 cut(s) 250, 817
MboII GAAGA 5 cut(s) 611, 629, 824, 827, 1103
MhlI GDGCHC 2 cut(s) 228, 417
MluCI AATT 2 cut(s) 770, 823
MlyI GAGTC 4 cut(s) 254, 287, 418, 463
MmeI TCCRAC 1 cut(s) 141
Mph1103I ATGCAT 1 cut(s) 616
MseI TTAA 3 cut(s) 609, 759, 1123
MslI CAYNNNNRTG 2 cut(s) 609, 963
MspI CCGG 2 cut(s) 674, 860
MspR9I CCNGG 5 cut(s) 44, 64, 443, 674, 860
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 3 cut(s) 44, 64, 443
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 99, 207, 272, 620
NciI CCSGG 2 cut(s) 674, 860
NcoI CCATGG 1 cut(s) 778
NdeI CATATG 1 cut(s) 1054
NdeII GATC 2 cut(s) 250, 817
NlaIII CATG 4 cut(s) 307, 494, 782, 1112
NlaIV GGNNCC 5 cut(s) 68, 390, 434, 858, 1106
NmeAIII GCCGAG 2 cut(s) 301, 337
NmuCI GTSAC 4 cut(s) 346, 503, 896, 1026
NsiI ATGCAT 1 cut(s) 616
PagI TCATGA 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 594
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 186
PctI GAATGC 1 cut(s) 559
PfeI GAWTC 1 cut(s) 499
PflFI GACNNNGTC 1 cut(s) 392
PkrI GCNGC 5 cut(s) 54, 101, 197, 200, 274
PleI GAGTC 4 cut(s) 254, 286, 418, 463
PpsI GAGTC 4 cut(s) 254, 286, 418, 463
Psp6I CCWGG 3 cut(s) 42, 62, 441
PspFI CCCAGC 2 cut(s) 282, 543
PspGI CCWGG 3 cut(s) 42, 62, 441
PspN4I GGNNCC 5 cut(s) 68, 390, 434, 858, 1106
PspPI GGNCC 4 cut(s) 66, 205, 388, 856
PstNI CAGNNNCTG 1 cut(s) 709
PsyI GACNNNGTC 1 cut(s) 392
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
RseI CAYNNNNRTG 2 cut(s) 609, 963
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 3 cut(s) 609, 759, 1123
SatI GCNGC 5 cut(s) 53, 100, 196, 199, 273
Sau3AI GATC 2 cut(s) 250, 817
Sau96I GGNCC 4 cut(s) 66, 205, 388, 856
SchI GAGTC 4 cut(s) 254, 287, 418, 463
ScrFI CCNGG 5 cut(s) 44, 64, 443, 674, 860
SduI GDGCHC 2 cut(s) 228, 417
SfaNI GCATC 2 cut(s) 610, 623
SinI GGWCC 3 cut(s) 66, 205, 388
SmiMI CAYNNNNRTG 2 cut(s) 609, 963
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 2 cut(s) 770, 823
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 5 cut(s) 42, 62, 441, 672, 858
StyI CCWWGG 2 cut(s) 375, 778
TaqI TCGA 7 cut(s) 168, 189, 231, 237, 243, 381, 396
TasI AATT 2 cut(s) 770, 823
TatI WGTACW 1 cut(s) 528
TauI GCSGC 4 cut(s) 102, 198, 201, 275
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 3 cut(s) 609, 759, 1123
Tru9I TTAA 3 cut(s) 609, 759, 1123
TscAI CASTG 2 cut(s) 710, 1035
TseFI GTSAC 4 cut(s) 346, 503, 896, 1026
TseI GCWGC 1 cut(s) 52
Tsp45I GTSAC 4 cut(s) 346, 503, 896, 1026
TspDTI ATGAA 4 cut(s) 292, 479, 825, 1104
TspGWI ACGGA 2 cut(s) 915, 1109
TspRI CASTG 2 cut(s) 710, 1035
Tth111I GACNNNGTC 1 cut(s) 392
VpaK11BI GGWCC 3 cut(s) 66, 205, 388
XagI CCTNNNNNAGG 1 cut(s) 440
XapI RAATTY 1 cut(s) 770
XcmI CCANNNNNNNNNTGG 2 cut(s) 40, 382
XspI CTAG 1 cut(s) 540
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.