MD00G1133600.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0017390
Physical Location & Seq
Reverse (-)
28980468 .. 28980950
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1133600.v1.1.491

Sequence Viewer

Length: 483 bp
ATGAACAAGGGTGCTTCTGGAATTATACGGAACTGTGAGGATAGCATTGTAATTTTGCAATTGAAATTTCTTCAGGTGGACCACATTCTAGGTGATAATCCACTGAGGATGTCGTTCATGGTTGGATACGGTGCATGCTACCCACAGAGGATACACCGCCTTGGCAGGTCATTACCGTCGGTGCATGCCCACCCGGCCCGCATCGGGTGCAAAGTCGGGTCTAGATACTTCTTAAGTCCAAAGCCCAACCCCAATTTGCTGGTGGGAGCCATCGTTGGTGAGCCCAACAGCTCAGACGAGTTCCCGGACTCGAGGGCCTTTCTTCCAAGAATCCAAGCCCACCACTTACATCAATTGGCCGTTGGTGGGCCTACTTTCATATTTTTCAGCCCACTATTGATTCCAACACCCAAATGTAAATTGCGACGAGTGTGGTGGTGCTCTAAGCTCGGTCACCCACTTACCCTGCCCCAATTGTTCTGA

Protein Analysis

161

Amino Acids

17.85

Weight (kDa)

9.91

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 23 - 105 1.4e-17 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017390)

Species Orthologous Gene IDs
malus_domestica MD00G1133600.v1.1
pyrus_communis pycom13g01910
rosa_chinensis RchiOBHm_Chr1g0374621 RchiOBHm_Chr2g0157001
rosa_laevigata RLG00000020957
rosa_multiflora Rmu_co8499365.1_g000001
rosa_rugosa Rorug01G0059700 Rorug07G0215400
rosa_samantha Rh1AG395300 Rh1CG373200 Rh1DG390700 Rh2AG523300 Rh2BG536500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 156
AciI CCGC 2 cut(s) 157, 199
AcoI YGGCCR 1 cut(s) 357
AcsI RAATTY 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 204, 366
AflII CTTAAG 1 cut(s) 232
AgsI TTSAA 1 cut(s) 64
AluBI AGCT 2 cut(s) 291, 448
AluI AGCT 2 cut(s) 291, 448
Alw21I GWGCWC 1 cut(s) 443
Ama87I CYCGRG 1 cut(s) 310
AoxI GGCC 4 cut(s) 195, 315, 357, 368
ApoI RAATTY 1 cut(s) 65
AspS9I GGNCC 4 cut(s) 79, 196, 315, 368
AsuC2I CCSGG 2 cut(s) 194, 305
AsuHPI GGTGA 3 cut(s) 104, 290, 446
AvaI CYCGRG 1 cut(s) 310
AvaII GGWCC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 443
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 344
BciVI GTATCC 2 cut(s) 119, 144
BcnI CCSGG 2 cut(s) 194, 305
BfaI CTAG 2 cut(s) 89, 222
BfrI CTTAAG 1 cut(s) 232
BfuAI ACCTGC 1 cut(s) 156
BfuI GTATCC 2 cut(s) 119, 144
BglI GCCNNNNNGGC 1 cut(s) 194
Bme1390I CCNGG 2 cut(s) 194, 305
Bme18I GGWCC 1 cut(s) 79
BmeT110I CYCGRG 1 cut(s) 310
BmgT120I GGNCC 4 cut(s) 79, 196, 315, 368
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 2 cut(s) 194, 305
BmsI GCATC 1 cut(s) 210
BpuMI CCSGG 2 cut(s) 194, 305
BsaJI CCNNGG 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 366
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 2 cut(s) 204, 366
BseMII CTCAG 2 cut(s) 95, 306
BshFI GGCC 4 cut(s) 197, 317, 359, 370
BsiHKAI GWGCWC 1 cut(s) 443
BsiHKCI CYCGRG 1 cut(s) 310
BsiSI CCGG 2 cut(s) 194, 305
BslI CCNNNNNNNGG 2 cut(s) 204, 366
BsnI GGCC 4 cut(s) 197, 317, 359, 370
BsoBI CYCGRG 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 285, 443
BspACI CCGC 2 cut(s) 157, 199
BspANI GGCC 4 cut(s) 197, 317, 359, 370
BspCNI CTCAG 2 cut(s) 96, 305
BspLI GGNNCC 1 cut(s) 268
BspMI ACCTGC 1 cut(s) 156
BspTI CTTAAG 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 160
BssT1I CCWWGG 1 cut(s) 160
Bst4CI ACNGT 3 cut(s) 35, 131, 177
BstAFI CTTAAG 1 cut(s) 232
BstAPI GCANNNNNTGC 1 cut(s) 207
BstC8I GCNNGC 3 cut(s) 136, 186, 199
BstDEI CTNAG 3 cut(s) 104, 292, 444
BstEII GGTNACC 1 cut(s) 452
BstF5I GGATG 1 cut(s) 114
BstMWI GCNNNNNNNGC 2 cut(s) 194, 207
BstNSI RCATGY 2 cut(s) 138, 188
BstPI GGTNACC 1 cut(s) 452
BstSCI CCNGG 2 cut(s) 192, 303
BstXI CCANNNNNNTGG 1 cut(s) 259
BsuI GTATCC 2 cut(s) 119, 144
BsuRI GGCC 4 cut(s) 197, 317, 359, 370
BtsCI GGATG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 101
BveI ACCTGC 1 cut(s) 156
Cac8I GCNNGC 3 cut(s) 136, 186, 199
Cfr13I GGNCC 4 cut(s) 79, 196, 315, 368
CviAII CATG 3 cut(s) 118, 135, 185
DdeI CTNAG 3 cut(s) 104, 292, 444
EaeI YGGCCR 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 310
Eco91I GGTNACC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 315
EcoO65I GGTNACC 1 cut(s) 452
EcoT14I CCWWGG 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 3 cut(s) 121, 138, 188
FaiI YATR 5 cut(s) 26, 119, 136, 186, 380
FatI CATG 3 cut(s) 117, 134, 184
FauI CCCGC 1 cut(s) 206
FokI GGATG 1 cut(s) 121
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 2 cut(s) 89, 222
HaeIII GGCC 4 cut(s) 197, 317, 359, 370
HapII CCGG 2 cut(s) 194, 305
Hin1II CATG 3 cut(s) 121, 138, 188
HinfI GANTC 3 cut(s) 308, 330, 400
HpaII CCGG 2 cut(s) 194, 305
HphI GGTGA 3 cut(s) 104, 290, 446
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 2 cut(s) 295, 482
Hpy188III TCNNGA 2 cut(s) 18, 222
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 181, 429
HpyCH4III ACNGT 3 cut(s) 35, 131, 177
HpyCH4V TGCA 4 cut(s) 58, 134, 184, 210
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 207
HpyF3I CTNAG 3 cut(s) 104, 292, 444
Hsp92II CATG 3 cut(s) 121, 138, 188
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 7 cut(s) 3, 59, 151, 207, 245, 318, 479
LweI GCATC 1 cut(s) 210
MaeI CTAG 2 cut(s) 89, 222
MaeIII GTNAC 1 cut(s) 452
MboII GAAGA 2 cut(s) 62, 314
MfeI CAATTG 3 cut(s) 59, 353, 473
MhlI GDGCHC 2 cut(s) 285, 443
MluCI AATT 8 cut(s) 21, 51, 59, 65, 253, 353, 419, 473
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 2 cut(s) 103, 428
MnlI CCTC 4 cut(s) 31, 99, 141, 306
MseI TTAA 1 cut(s) 233
MslI CAYNNNNRTG 1 cut(s) 412
MspCI CTTAAG 1 cut(s) 232
MspI CCGG 2 cut(s) 194, 305
MspR9I CCNGG 2 cut(s) 194, 305
MunI CAATTG 3 cut(s) 59, 353, 473
MwoI GCNNNNNNNGC 2 cut(s) 194, 207
NciI CCSGG 2 cut(s) 194, 305
NlaIII CATG 3 cut(s) 121, 138, 188
NlaIV GGNNCC 1 cut(s) 268
NmuCI GTSAC 1 cut(s) 452
NspI RCATGY 2 cut(s) 138, 188
PaeI GCATGC 2 cut(s) 138, 188
PaeR7I CTCGAG 1 cut(s) 310
PfeI GAWTC 2 cut(s) 330, 400
PfoI TCCNGGA 1 cut(s) 303
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PspEI GGTNACC 1 cut(s) 452
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 4 cut(s) 79, 196, 315, 368
PspXI VCTCGAGB 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 412
SaqAI TTAA 1 cut(s) 233
Sau96I GGNCC 4 cut(s) 79, 196, 315, 368
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 2 cut(s) 194, 305
SduI GDGCHC 2 cut(s) 285, 443
SetI ASST 5 cut(s) 78, 94, 170, 293, 450
SfaNI GCATC 1 cut(s) 210
Sfr274I CTCGAG 1 cut(s) 310
SinI GGWCC 1 cut(s) 79
SlaI CTCGAG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 412
SmlI CTYRAG 2 cut(s) 232, 310
SmoI CTYRAG 2 cut(s) 232, 310
SphI GCATGC 2 cut(s) 138, 188
Sse9I AATT 8 cut(s) 21, 51, 59, 65, 253, 353, 419, 473
SsiI CCGC 2 cut(s) 157, 199
SspMI CTAG 2 cut(s) 89, 222
StyD4I CCNGG 2 cut(s) 192, 303
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 3 cut(s) 35, 131, 177
TaqI TCGA 1 cut(s) 311
TaqII GACCGA 1 cut(s) 440
TasI AATT 8 cut(s) 21, 51, 59, 65, 253, 353, 419, 473
TfiI GAWTC 2 cut(s) 330, 400
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TscAI CASTG 1 cut(s) 108
TseFI GTSAC 1 cut(s) 452
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 3 cut(s) 17, 106, 367
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 1 cut(s) 65
XbaI TCTAGA 1 cut(s) 221
XceI RCATGY 2 cut(s) 138, 188
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 310
XspI CTAG 2 cut(s) 89, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.