Rh1CG373200

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0017390
Physical Location & Seq
Forward (+)
64252353 .. 64259684
7332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG373200.1

Sequence Viewer

Length: 555 bp
ATGAATTTGGAGTCCAATGTAGTCCTAAGAAATGTGGAAGGCCAAGCAGTTGCATACAATGGCGGAGATGTTACCGAATTTGGATGGGATGCAAAGCCTGCTGGCATTAACATCCTTTGGGTGATGACTAACCCCGTCGAGGCCAATCCCTTCATTCCCAAAGCGGATGGGCTTGTTTGCTCCATTGTGCCCCCGAATCACCTGCTCCCAAATCAGTCACATACTCAGGACTCAGGAGTTTATGCTGGCTATATGAAAGCTGCAAGAAAAGTTGTAAATTGTGGGAACAATGTTGTTGTCACTCCAGCTAGGCTGGTGGAACTCACAAAAGGACAGGTTGATTATATACTGGGAAGCAATCCTAATAGCATGTCATACATGGTGGGATATGGTACAAGATTTCCCCAGAAGACACCTCACCACGGATCGGTGATACCTTCGATTGATCAACACCCCAAACACATGCAGTGTCATGAAGGAGACGTATTTCAAAACTCAGGCTCCAAATCCAAACTTGCTAATAGGTGTAATCGTTGGAGGACCAGCTCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

184

Amino Acids

20.04

Weight (kDa)

8.69

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 19 - 151 1.5e-16 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017390)

Species Orthologous Gene IDs
malus_domestica MD00G1133600.v1.1
pyrus_communis pycom13g01910
rosa_chinensis RchiOBHm_Chr1g0374621 RchiOBHm_Chr2g0157001
rosa_laevigata RLG00000020957
rosa_multiflora Rmu_co8499365.1_g000001
rosa_rugosa Rorug01G0059700 Rorug07G0215400
rosa_samantha Rh1AG395300 Rh1CG373200 Rh1DG390700 Rh2AG523300 Rh2BG536500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 210
AciI CCGC 2 cut(s) 63, 164
AclWI GGATC 1 cut(s) 433
AcsI RAATTY 2 cut(s) 4, 77
AfaI GTAC 1 cut(s) 394
AfiI CCNNNNNNNGG 3 cut(s) 139, 422, 427
AgsI TTSAA 1 cut(s) 491
AluBI AGCT 3 cut(s) 260, 308, 546
AluI AGCT 3 cut(s) 260, 308, 546
Alw26I GTCTC 1 cut(s) 474
AlwI GGATC 1 cut(s) 433
AoxI GGCC 2 cut(s) 40, 141
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 2 cut(s) 4, 77
AspS9I GGNCC 1 cut(s) 540
AsuHPI GGTGA 4 cut(s) 133, 191, 410, 442
AvaII GGWCC 1 cut(s) 540
BaeGI GKGCMC 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 416
BbvI GCAGC 1 cut(s) 247
BccI CCATC 2 cut(s) 78, 161
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BclI TGATCA 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 474
BfaI CTAG 1 cut(s) 309
BfuAI ACCTGC 1 cut(s) 210
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme18I GGWCC 1 cut(s) 540
BmgT120I GGNCC 1 cut(s) 540
BmiI GGNNCC 1 cut(s) 502
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 1 cut(s) 79
BmuI ACTGGG 1 cut(s) 359
BpiI GAAGAC 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 421
BsaXI ACNNNNNCTCC 2 cut(s) 485, 515
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 422, 427
Bse1I ACTGG 1 cut(s) 354
BseDI CCNNGG 1 cut(s) 421
BseGI GGATG 4 cut(s) 89, 94, 111, 172
BseLI CCNNNNNNNGG 3 cut(s) 139, 422, 427
BseMII CTCAG 3 cut(s) 239, 246, 510
BseNI ACTGG 1 cut(s) 354
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 1 cut(s) 247
BshFI GGCC 2 cut(s) 42, 143
BslI CCNNNNNNNGG 3 cut(s) 139, 422, 427
BsmAI GTCTC 1 cut(s) 474
BsmBI CGTCTC 1 cut(s) 474
BsnI GGCC 2 cut(s) 42, 143
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 2 cut(s) 425, 445
BspACI CCGC 2 cut(s) 63, 164
BspANI GGCC 2 cut(s) 42, 143
BspCNI CTCAG 3 cut(s) 238, 245, 509
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 1 cut(s) 502
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 1 cut(s) 433
BsrI ACTGG 1 cut(s) 354
BssECI CCNNGG 1 cut(s) 421
BssMI GATC 2 cut(s) 425, 445
BstAPI GCANNNNNTGC 1 cut(s) 98
BstC8I GCNNGC 3 cut(s) 99, 103, 247
BstDEI CTNAG 4 cut(s) 26, 225, 232, 496
BstDSI CCRYGG 1 cut(s) 421
BstF5I GGATG 4 cut(s) 89, 94, 111, 172
BstKTI GATC 2 cut(s) 428, 448
BstMAI GTCTC 1 cut(s) 474
BstMBI GATC 2 cut(s) 425, 445
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNSI RCATGY 2 cut(s) 373, 466
BstSLI GKGCMC 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 247
BstV2I GAAGAC 1 cut(s) 416
BsuRI GGCC 2 cut(s) 42, 143
BtgI CCRYGG 1 cut(s) 421
BtsCI GGATG 4 cut(s) 89, 94, 111, 172
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 473
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 99, 103, 247
CciI TCATGA 1 cut(s) 472
Cfr13I GGNCC 1 cut(s) 540
Csp6I GTAC 1 cut(s) 393
CviAII CATG 4 cut(s) 370, 379, 463, 473
CviQI GTAC 1 cut(s) 393
DdeI CTNAG 4 cut(s) 26, 225, 232, 496
DpnI GATC 2 cut(s) 427, 447
DpnII GATC 2 cut(s) 425, 445
EciI GGCGGA 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 540
Esp3I CGTCTC 1 cut(s) 474
FaeI CATG 4 cut(s) 373, 382, 466, 476
FatI CATG 4 cut(s) 369, 378, 462, 472
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 1 cut(s) 261
FokI GGATG 4 cut(s) 96, 98, 101, 179
Fsp4HI GCNGC 1 cut(s) 261
FspBI CTAG 1 cut(s) 309
GluI GCNGC 1 cut(s) 261
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 2 cut(s) 42, 143
Hin1II CATG 4 cut(s) 373, 382, 466, 476
HinfI GANTC 3 cut(s) 11, 196, 230
HphI GGTGA 4 cut(s) 133, 191, 410, 442
Hpy188III TCNNGA 4 cut(s) 227, 234, 473, 549
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 4 cut(s) 32, 160, 447, 470
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 4 cut(s) 53, 92, 263, 466
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 4 cut(s) 26, 225, 232, 496
HpySE526I ACGT 1 cut(s) 483
Hsp92II CATG 4 cut(s) 373, 382, 466, 476
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 2 cut(s) 425, 445
LmnI GCTCC 3 cut(s) 185, 210, 506
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 1 cut(s) 79
MaeI CTAG 1 cut(s) 309
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 3 cut(s) 70, 216, 298
MalI GATC 2 cut(s) 427, 447
MboI GATC 2 cut(s) 425, 445
MboII GAAGA 1 cut(s) 421
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 3 cut(s) 4, 77, 277
MlyI GAGTC 2 cut(s) 20, 224
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 3 cut(s) 133, 426, 531
MseI TTAA 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 98
NdeII GATC 2 cut(s) 425, 445
NlaIII CATG 4 cut(s) 373, 382, 466, 476
NlaIV GGNNCC 1 cut(s) 502
NmuCI GTSAC 2 cut(s) 216, 298
NspI RCATGY 2 cut(s) 373, 466
PagI TCATGA 1 cut(s) 472
PaqCI CACCTGC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 196
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 2 cut(s) 19, 224
PpsI GAGTC 2 cut(s) 19, 224
PspN4I GGNNCC 1 cut(s) 502
PspPI GGNCC 1 cut(s) 540
RsaI GTAC 1 cut(s) 394
RsaNI GTAC 1 cut(s) 393
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 2 cut(s) 425, 445
Sau96I GGNCC 1 cut(s) 540
SchI GAGTC 2 cut(s) 20, 224
SduI GDGCHC 1 cut(s) 192
SetI ASST 9 cut(s) 204, 262, 310, 339, 418, 439, 486, 527, 548
SfaNI GCATC 1 cut(s) 79
SinI GGWCC 1 cut(s) 540
Sse9I AATT 3 cut(s) 4, 77, 277
SsiI CCGC 2 cut(s) 63, 164
SspMI CTAG 1 cut(s) 309
TaiI ACGT 1 cut(s) 486
TaqI TCGA 2 cut(s) 138, 440
TasI AATT 3 cut(s) 4, 77, 277
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 1 cut(s) 473
TseFI GTSAC 2 cut(s) 216, 298
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 2 cut(s) 216, 298
TspDTI ATGAA 4 cut(s) 17, 142, 269, 489
TspGWI ACGGA 1 cut(s) 438
TspRI CASTG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 540
XapI RAATTY 2 cut(s) 4, 77
XceI RCATGY 2 cut(s) 373, 466
XspI CTAG 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.