Rh2BG536500

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017390
Physical Location & Seq
Reverse (-)
74524101 .. 74525644
1544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG536500.1

Sequence Viewer

Length: 495 bp
ATGATCGACTTCACCAAAGGACAGGTTGACTATATACTAGGAAGCAACCCATTGGGAATGTCATACATGGTGGGATATGGTTCAAAGTTTCCAAAGAGAATACACCACCGCGCTTCGGTGCTACGTTCTATGGATCAGCAGCCGGGCAACATTGAATGTCACGCAGGAACGCCATATTTCGAGACTCAAGCTCCAAATCAGAGCTTGCTAATAGGCGCGATTGTGGGAGGACCTGCTGAGAACGATTCTTTCGAAGACTCTCGGTACAATGTGTACAATATAGATCTAAGAGAGGACGTGTCAGATGCTGGAAAATGGATTCAGAGATGCTCCGACGTTGGTTTCAGGGAGAAGGCAGCGATGAGACGCCGTTGGCTCCTCAGTGATCAGTGGGAGAGTCCTTCGTCTTCTCCGGATTATTCACAAGTTAGTATCAAACACTTGCGTGCATACACTGTAGTTGCAGTGTTCTTTTTGGATATCTATTGGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

164

Amino Acids

18.64

Weight (kDa)

6.06

Isoelectric Point (pI)

62.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 3 - 90 4.4e-26 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017390)

Species Orthologous Gene IDs
malus_domestica MD00G1133600.v1.1
pyrus_communis pycom13g01910
rosa_chinensis RchiOBHm_Chr1g0374621 RchiOBHm_Chr2g0157001
rosa_laevigata RLG00000020957
rosa_multiflora Rmu_co8499365.1_g000001
rosa_rugosa Rorug01G0059700 Rorug07G0215400
rosa_samantha Rh1AG395300 Rh1CG373200 Rh1DG390700 Rh2AG523300 Rh2BG536500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 241
AccII CGCG 2 cut(s) 111, 218
AccIII TCCGGA 1 cut(s) 412
AciI CCGC 1 cut(s) 109
AclWI GGATC 1 cut(s) 141
AcyI GRCGYC 1 cut(s) 367
AfaI GTAC 2 cut(s) 266, 275
AfiI CCNNNNNNNGG 1 cut(s) 115
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 2 cut(s) 84, 155
AjiI CACGTC 1 cut(s) 298
AleI CACNNNNGTG 1 cut(s) 444
AluBI AGCT 2 cut(s) 191, 204
AluI AGCT 2 cut(s) 191, 204
Alw26I GTCTC 2 cut(s) 176, 358
AlwI GGATC 1 cut(s) 141
AlwNI CAGNNNCTG 1 cut(s) 308
Aor13HI TCCGGA 1 cut(s) 412
AoxI GGCC 1 cut(s) 489
ApeKI GCWGC 2 cut(s) 139, 356
AspLEI GCGC 2 cut(s) 113, 218
AspS9I GGNCC 2 cut(s) 230, 489
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 4
AsuII TTCGAA 1 cut(s) 252
AvaII GGWCC 1 cut(s) 230
BbsI GAAGAC 2 cut(s) 261, 399
BbvI GCAGC 2 cut(s) 151, 368
BceAI ACGGC 1 cut(s) 354
BclI TGATCA 1 cut(s) 385
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 2 cut(s) 176, 358
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 456
BfuAI ACCTGC 1 cut(s) 241
BglII AGATCT 1 cut(s) 283
BisI GCNGC 2 cut(s) 140, 357
BlsI GCNGC 2 cut(s) 141, 358
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 230
BmgBI CACGTC 1 cut(s) 298
BmgT120I GGNCC 2 cut(s) 230, 489
BmiI GGNNCC 1 cut(s) 377
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 2 cut(s) 295, 317
BpiI GAAGAC 2 cut(s) 261, 399
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 367
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 1 cut(s) 115
BseAI TCCGGA 1 cut(s) 412
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMII CTCAG 2 cut(s) 228, 394
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 2 cut(s) 151, 368
Bsh1236I CGCG 2 cut(s) 111, 218
BshFI GGCC 1 cut(s) 491
BsiSI CCGG 2 cut(s) 143, 413
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 2 cut(s) 176, 358
BsmBI CGTCTC 1 cut(s) 358
BsnI GGCC 1 cut(s) 491
Bsp119I TTCGAA 1 cut(s) 252
Bsp13I TCCGGA 1 cut(s) 412
Bsp1407I TGTACA 1 cut(s) 273
Bsp143I GATC 4 cut(s) 3, 133, 283, 385
BspACI CCGC 1 cut(s) 109
BspANI GGCC 1 cut(s) 491
BspCNI CTCAG 2 cut(s) 229, 393
BspEI TCCGGA 1 cut(s) 412
BspFNI CGCG 2 cut(s) 111, 218
BspLI GGNNCC 1 cut(s) 377
BspMI ACCTGC 1 cut(s) 241
BspPI GGATC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 252
BsrGI TGTACA 1 cut(s) 273
BssMI GATC 4 cut(s) 3, 133, 283, 385
BssNI GRCGYC 1 cut(s) 367
Bst4CI ACNGT 1 cut(s) 457
BstACI GRCGYC 1 cut(s) 367
BstAUI TGTACA 1 cut(s) 273
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 2 cut(s) 206, 447
BstDEI CTNAG 3 cut(s) 237, 287, 380
BstFNI CGCG 2 cut(s) 111, 218
BstHHI GCGC 2 cut(s) 113, 218
BstKTI GATC 4 cut(s) 6, 136, 286, 388
BstMAI GTCTC 2 cut(s) 176, 358
BstMBI GATC 4 cut(s) 3, 133, 283, 385
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 2 cut(s) 111, 218
BstV1I GCAGC 2 cut(s) 151, 368
BstV2I GAAGAC 2 cut(s) 261, 399
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 1 cut(s) 491
BtgZI GCGATG 1 cut(s) 374
BtrI CACGTC 1 cut(s) 298
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 4 cut(s) 388, 395, 453, 471
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 2 cut(s) 206, 447
CaiI CAGNNNCTG 1 cut(s) 308
CfoI GCGC 2 cut(s) 113, 218
Cfr13I GGNCC 2 cut(s) 230, 489
CseI GACGC 1 cut(s) 375
Csp6I GTAC 2 cut(s) 265, 274
CviAII CATG 1 cut(s) 67
CviJI RGCY 5 cut(s) 142, 191, 204, 376, 491
CviKI_1 RGCY 5 cut(s) 142, 191, 204, 376, 491
CviQI GTAC 2 cut(s) 265, 274
DdeI CTNAG 3 cut(s) 237, 287, 380
DpnI GATC 4 cut(s) 5, 135, 285, 387
DpnII GATC 4 cut(s) 3, 133, 283, 385
Eco32I GATATC 1 cut(s) 481
Eco47I GGWCC 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 230
EcoRV GATATC 1 cut(s) 481
Esp3I CGTCTC 1 cut(s) 358
FaeI CATG 1 cut(s) 70
FaiI YATR 9 cut(s) 33, 35, 64, 68, 78, 131, 175, 281, 451
FatI CATG 1 cut(s) 66
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 2 cut(s) 140, 357
Fsp4HI GCNGC 2 cut(s) 140, 357
FspBI CTAG 1 cut(s) 38
GlaI GCGC 2 cut(s) 112, 217
GluI GCNGC 2 cut(s) 140, 357
HaeIII GGCC 1 cut(s) 491
HapII CCGG 2 cut(s) 143, 413
HgaI GACGC 1 cut(s) 375
HhaI GCGC 2 cut(s) 113, 218
Hin1I GRCGYC 1 cut(s) 367
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 2 cut(s) 111, 216
HinP1I GCGC 2 cut(s) 111, 216
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 5 cut(s) 184, 245, 257, 319, 397
HpaII CCGG 2 cut(s) 143, 413
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 2 cut(s) 28, 274
Hpy188I TCNGA 4 cut(s) 201, 304, 324, 334
Hpy188III TCNNGA 2 cut(s) 181, 413
Hpy8I GTNNAC 2 cut(s) 28, 274
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 2 cut(s) 346, 411
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 3 cut(s) 124, 297, 336
HpyCH4V TGCA 2 cut(s) 449, 464
HpyF3I CTNAG 3 cut(s) 237, 287, 380
HpySE526I ACGT 3 cut(s) 124, 297, 336
Hsp92I GRCGYC 1 cut(s) 367
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 2 cut(s) 111, 216
Kpn2I TCCGGA 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 4 cut(s) 3, 133, 283, 385
LmnI GCTCC 3 cut(s) 196, 335, 381
LpnPI CCDG 7 cut(s) 8, 150, 156, 246, 294, 331, 426
Lsp1109I GCAGC 2 cut(s) 151, 368
LweI GCATC 2 cut(s) 295, 317
MaeI CTAG 1 cut(s) 38
MaeII ACGT 3 cut(s) 124, 297, 336
MaeIII GTNAC 1 cut(s) 158
MalI GATC 4 cut(s) 5, 135, 285, 387
MboI GATC 4 cut(s) 3, 133, 283, 385
MboII GAAGA 2 cut(s) 266, 399
MflI RGATCY 1 cut(s) 283
MlyI GAGTC 3 cut(s) 178, 251, 406
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 3 cut(s) 221, 286, 389
MroI TCCGGA 1 cut(s) 412
MslI CAYNNNNRTG 1 cut(s) 444
MspI CCGG 2 cut(s) 143, 413
MspR9I CCNGG 1 cut(s) 144
MvnI CGCG 2 cut(s) 111, 218
NciI CCSGG 1 cut(s) 144
NdeII GATC 4 cut(s) 3, 133, 283, 385
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 377
NmuCI GTSAC 1 cut(s) 158
NspV TTCGAA 1 cut(s) 252
OliI CACNNNNGTG 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 2 cut(s) 245, 319
PkrI GCNGC 2 cut(s) 141, 358
PleI GAGTC 3 cut(s) 178, 251, 405
PpsI GAGTC 3 cut(s) 178, 251, 405
PpuMI RGGWCCY 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 230
PspN4I GGNNCC 1 cut(s) 377
PspPI GGNCC 2 cut(s) 230, 489
PspPPI RGGWCCY 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 1 cut(s) 283
RsaI GTAC 2 cut(s) 266, 275
RsaNI GTAC 2 cut(s) 265, 274
RseI CAYNNNNRTG 1 cut(s) 444
SatI GCNGC 2 cut(s) 140, 357
Sau3AI GATC 4 cut(s) 3, 133, 283, 385
Sau96I GGNCC 2 cut(s) 230, 489
SchI GAGTC 3 cut(s) 178, 251, 406
ScrFI CCNGG 1 cut(s) 144
SetI ASST 7 cut(s) 27, 127, 193, 206, 235, 300, 339
SfaNI GCATC 2 cut(s) 295, 317
SfcI CTRYAG 1 cut(s) 456
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 444
SmlI CTYRAG 1 cut(s) 186
SmoI CTYRAG 1 cut(s) 186
SsiI CCGC 1 cut(s) 109
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 3 cut(s) 127, 300, 339
TaqI TCGA 3 cut(s) 6, 180, 252
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 2 cut(s) 245, 319
TscAI CASTG 4 cut(s) 388, 395, 460, 471
TseFI GTSAC 1 cut(s) 158
TseI GCWGC 2 cut(s) 139, 356
Tsp45I GTSAC 1 cut(s) 158
TspRI CASTG 4 cut(s) 388, 395, 460, 471
VpaK11BI GGWCC 1 cut(s) 230
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.