Rh2AG523300

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0017390
Physical Location & Seq
Reverse (-)
75037595 .. 75039046
1452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG523300.1

Sequence Viewer

Length: 426 bp
ATGGTGGGATATGGTTCAAAGTTTCCAAAGAGAATACACCACCGCGCTTCGGTGCTACCTGCTTCTATGGATCAGCAGCCGGGGAACATTGAATGTCATGCAGGAACGCCATATTTCGAGACTCAAGCTCCAAATCAGAACTTGCTAATAGGCGCGATTGTGGGAGGACCTGCTGAGAACGATTCTTTCGAAGACTCTCGGTACAATGTGTACAATATAGATCTAAGAAAGGACGCGTCAGATGCTGGAAAATGGATTCAGAGATGCTCCGACGTTGGTTTCAGGGAGAAGGCAGCGATGAGACGCTGTTGGCTCCTCATTGATTCGTGGGAGAGTCCTTCGTCTTCTCAAGATTATTCACAAGTTAGTATCAAACACTTGCGTGCATACACTGTAGTTGCAGTGTTTTTTTTGGATATCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

141

Amino Acids

15.92

Weight (kDa)

6.4

Isoelectric Point (pI)

56.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 69 2.8e-13 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017390)

Species Orthologous Gene IDs
malus_domestica MD00G1133600.v1.1
pyrus_communis pycom13g01910
rosa_chinensis RchiOBHm_Chr1g0374621 RchiOBHm_Chr2g0157001
rosa_laevigata RLG00000020957
rosa_multiflora Rmu_co8499365.1_g000001
rosa_rugosa Rorug01G0059700 Rorug07G0215400
rosa_samantha Rh1AG395300 Rh1CG373200 Rh1DG390700 Rh2AG523300 Rh2BG536500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 67, 178
AccII CGCG 3 cut(s) 45, 155, 236
AciI CCGC 1 cut(s) 43
AclWI GGATC 1 cut(s) 78
AfaI GTAC 2 cut(s) 203, 212
AfiI CCNNNNNNNGG 1 cut(s) 49
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 2 cut(s) 18, 92
AleI CACNNNNGTG 1 cut(s) 381
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
Alw26I GTCTC 2 cut(s) 113, 295
AlwI GGATC 1 cut(s) 78
AlwNI CAGNNNCTG 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 76, 293
AspLEI GCGC 2 cut(s) 47, 155
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 81
AsuII TTCGAA 1 cut(s) 189
AvaII GGWCC 1 cut(s) 167
BbsI GAAGAC 2 cut(s) 198, 336
BbvI GCAGC 2 cut(s) 88, 305
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 113, 295
BfmI CTRYAG 1 cut(s) 393
BfuAI ACCTGC 2 cut(s) 67, 178
BglII AGATCT 1 cut(s) 220
BisI GCNGC 2 cut(s) 77, 294
BlsI GCNGC 2 cut(s) 78, 295
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 314
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 232, 254
BpiI GAAGAC 2 cut(s) 198, 336
Bpu14I TTCGAA 1 cut(s) 189
BpuEI CTTGAG 2 cut(s) 108, 333
BpuMI CCSGG 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 80
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 1 cut(s) 49
BseDI CCNNGG 1 cut(s) 80
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMII CTCAG 1 cut(s) 165
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 2 cut(s) 88, 305
Bsh1236I CGCG 3 cut(s) 45, 155, 236
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 2 cut(s) 113, 295
BsmBI CGTCTC 1 cut(s) 295
Bsp119I TTCGAA 1 cut(s) 189
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 2 cut(s) 70, 220
BspACI CCGC 1 cut(s) 43
BspCNI CTCAG 1 cut(s) 166
BspFNI CGCG 3 cut(s) 45, 155, 236
BspLI GGNNCC 1 cut(s) 314
BspMI ACCTGC 2 cut(s) 67, 178
BspPI GGATC 1 cut(s) 78
BspT104I TTCGAA 1 cut(s) 189
BsrGI TGTACA 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 2 cut(s) 70, 220
Bst4CI ACNGT 1 cut(s) 394
BstAUI TGTACA 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 174, 224
BstFNI CGCG 3 cut(s) 45, 155, 236
BstHHI GCGC 2 cut(s) 47, 155
BstKTI GATC 2 cut(s) 73, 223
BstMAI GTCTC 2 cut(s) 113, 295
BstMBI GATC 2 cut(s) 70, 220
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 393
BstUI CGCG 3 cut(s) 45, 155, 236
BstV1I GCAGC 2 cut(s) 88, 305
BstV2I GAAGAC 2 cut(s) 198, 336
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BtgZI GCGATG 1 cut(s) 311
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 2 cut(s) 390, 408
BveI ACCTGC 2 cut(s) 67, 178
Cac8I GCNNGC 1 cut(s) 384
CaiI CAGNNNCTG 1 cut(s) 245
CfoI GCGC 2 cut(s) 47, 155
Cfr13I GGNCC 1 cut(s) 167
CseI GACGC 3 cut(s) 225, 242, 312
Csp6I GTAC 2 cut(s) 202, 211
CviAII CATG 1 cut(s) 98
CviJI RGCY 3 cut(s) 79, 128, 313
CviKI_1 RGCY 3 cut(s) 79, 128, 313
CviQI GTAC 2 cut(s) 202, 211
DdeI CTNAG 2 cut(s) 174, 224
DpnI GATC 2 cut(s) 72, 222
DpnII GATC 2 cut(s) 70, 220
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 167
EcoRV GATATC 1 cut(s) 418
Esp3I CGTCTC 1 cut(s) 295
FaeI CATG 1 cut(s) 101
FaiI YATR 6 cut(s) 12, 68, 99, 112, 218, 388
FatI CATG 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 77, 294
Fsp4HI GCNGC 2 cut(s) 77, 294
GlaI GCGC 2 cut(s) 46, 154
GluI GCNGC 2 cut(s) 77, 294
HapII CCGG 1 cut(s) 80
HgaI GACGC 3 cut(s) 225, 242, 312
HhaI GCGC 2 cut(s) 47, 155
Hin1II CATG 1 cut(s) 101
Hin6I GCGC 2 cut(s) 45, 153
HinP1I GCGC 2 cut(s) 45, 153
HinfI GANTC 6 cut(s) 121, 182, 194, 256, 323, 334
HpaII CCGG 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 4 cut(s) 138, 241, 261, 271
Hpy188III TCNNGA 2 cut(s) 118, 350
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 2 cut(s) 283, 348
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 3 cut(s) 101, 386, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 2 cut(s) 174, 224
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 1 cut(s) 101
HspAI GCGC 2 cut(s) 45, 153
Kzo9I GATC 2 cut(s) 70, 220
LmnI GCTCC 3 cut(s) 133, 272, 318
LpnPI CCDG 6 cut(s) 72, 87, 93, 183, 231, 268
Lsp1109I GCAGC 2 cut(s) 88, 305
LweI GCATC 2 cut(s) 232, 254
MaeII ACGT 1 cut(s) 273
MalI GATC 2 cut(s) 72, 222
MboI GATC 2 cut(s) 70, 220
MboII GAAGA 2 cut(s) 203, 336
MflI RGATCY 1 cut(s) 220
MluI ACGCGT 1 cut(s) 234
MlyI GAGTC 3 cut(s) 115, 188, 343
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 2 cut(s) 158, 326
MslI CAYNNNNRTG 1 cut(s) 381
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 81
MvnI CGCG 3 cut(s) 45, 155, 236
MwoI GCNNNNNNNGC 1 cut(s) 242
NciI CCSGG 1 cut(s) 81
NdeII GATC 2 cut(s) 70, 220
NlaIII CATG 1 cut(s) 101
NlaIV GGNNCC 1 cut(s) 314
NspV TTCGAA 1 cut(s) 189
OliI CACNNNNGTG 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 3 cut(s) 182, 256, 323
PkrI GCNGC 2 cut(s) 78, 295
PleI GAGTC 3 cut(s) 115, 188, 342
PpsI GAGTC 3 cut(s) 115, 188, 342
PpuMI RGGWCCY 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 314
PspPI GGNCC 1 cut(s) 167
PspPPI RGGWCCY 1 cut(s) 167
PstNI CAGNNNCTG 1 cut(s) 245
PsuI RGATCY 1 cut(s) 220
RsaI GTAC 2 cut(s) 203, 212
RsaNI GTAC 2 cut(s) 202, 211
RseI CAYNNNNRTG 1 cut(s) 381
SatI GCNGC 2 cut(s) 77, 294
Sau3AI GATC 2 cut(s) 70, 220
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 3 cut(s) 115, 188, 343
ScrFI CCNGG 1 cut(s) 81
SetI ASST 4 cut(s) 61, 130, 172, 276
SfaNI GCATC 2 cut(s) 232, 254
SfcI CTRYAG 1 cut(s) 393
SfuI TTCGAA 1 cut(s) 189
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 2 cut(s) 123, 348
SmoI CTYRAG 2 cut(s) 123, 348
SsiI CCGC 1 cut(s) 43
StyD4I CCNGG 1 cut(s) 79
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 117, 189
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 3 cut(s) 182, 256, 323
TscAI CASTG 2 cut(s) 397, 408
TseI GCWGC 2 cut(s) 76, 293
TspRI CASTG 2 cut(s) 397, 408
VpaK11BI GGWCC 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.