Rorug07G0215400

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0017390
Physical Location & Seq
Forward (+)
19106228 .. 19109472
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0215400.1

Sequence Viewer

Length: 765 bp
ATGTCCATTTCCATGTCCTTGTTCTCTCCGTCAACCCATCTCCCAACTCTCTCCCCCTCCTCCTCCTCCTCCTCATACCCAAAACCCCATTTCACCCTCAAGCCTTGTCTCTCTCTCAGACCCAAGACCCCTCTCTTCACCACCAGAGCCTCAGCTGAAAATGGTGCAGGAGCTGCTGCTGCCACAGCTGTGGAGGCAGAGCCCAAGGTCCCCGAGCCGGTTGCGGCCAAGAGTGAGAGCTCTAATGGGTCAGCTGGGCCGGCTGAGGAGGAGGTGAAGGTGGTCAGTTTGTTTGAGGACGCCAGATGGGTCAATGGGACTTGGGACTTGAAGCAGTTTGAGAAAAGTGGCAAGACTGACTGGGATGCTGTTATTGATGCTGAGGCTAGGAGGAGAAAATGGCTCCAGGATAACCCAGAATCGTCCAGTAATGATAACCCTATACTTTTCGACACCTCAATTGTACCTTGGTGGGCATGGGTAAAGAGATACCATTTACCTGAAGCTGAGCTACTCAATGGTCGTGCTGCCATGGTAGGTTTCTTCAGTGCTTATCTGGTTGATAGCTTGACTGGAGTGGGCCTAGTTGATCAAATGGGCAATTTCGTCTGCAAAACATTACTGTTTGTAGCTGTGGCGGGAGTACTTCTGATCCGCAAGAACGAGGATGTTGAAAAACTAAAGAAACTGCTGGATGAGACAACATTTTACGACAAGCAATGGCAGGCAACCTGGCAGGATGAGACTCCTGGCAATCCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

254

Amino Acids

27.98

Weight (kDa)

5.37

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017390)

Species Orthologous Gene IDs
malus_domestica MD00G1133600.v1.1
pyrus_communis pycom13g01910
rosa_chinensis RchiOBHm_Chr1g0374621 RchiOBHm_Chr2g0157001
rosa_laevigata RLG00000020957
rosa_multiflora Rmu_co8499365.1_g000001
rosa_rugosa Rorug01G0059700 Rorug07G0215400
rosa_samantha Rh1AG395300 Rh1CG373200 Rh1DG390700 Rh2AG523300 Rh2BG536500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 224, 638, 655
AclWI GGATC 1 cut(s) 646
AcoI YGGCCR 1 cut(s) 225
AcuI CTGAAG 2 cut(s) 522, 529
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 2 cut(s) 465, 645
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 2 cut(s) 331, 674
AjnI CCWGG 3 cut(s) 405, 731, 748
AleI CACNNNNGTG 1 cut(s) 188
AluBI AGCT 9 cut(s) 155, 173, 188, 240, 254, 506, 511, 567, 632
AluI AGCT 9 cut(s) 155, 173, 188, 240, 254, 506, 511, 567, 632
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 3 cut(s) 113, 692, 737
AlwI GGATC 1 cut(s) 646
AlwNI CAGNNNCTG 1 cut(s) 173
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 3 cut(s) 225, 257, 580
ApeKI GCWGC 4 cut(s) 173, 176, 179, 527
AspS9I GGNCC 3 cut(s) 208, 257, 580
AsuHPI GGTGA 3 cut(s) 85, 130, 286
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 1 cut(s) 208
BanII GRGCYC 2 cut(s) 204, 242
BarI GAAGNNNNNNTAC 2 cut(s) 495, 527
Bbv12I GWGCWC 1 cut(s) 242
BbvCI CCTCAGC 3 cut(s) 151, 264, 381
BbvI GCAGC 4 cut(s) 160, 163, 166, 514
BccI CCATC 2 cut(s) 45, 300
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 3 cut(s) 407, 733, 750
BclI TGATCA 1 cut(s) 589
BcoDI GTCTC 3 cut(s) 113, 692, 737
BfaI CTAG 2 cut(s) 387, 584
BisI GCNGC 5 cut(s) 174, 177, 180, 225, 528
BlpI GCTNAGC 1 cut(s) 507
BlsI GCNGC 5 cut(s) 175, 178, 181, 226, 529
BmcAI AGTACT 1 cut(s) 645
Bme1390I CCNGG 3 cut(s) 407, 733, 750
Bme18I GGWCC 1 cut(s) 208
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 3 cut(s) 208, 257, 580
BmiI GGNNCC 2 cut(s) 210, 404
BmrFI CCNGG 3 cut(s) 407, 733, 750
BmrI ACTGGG 1 cut(s) 370
BmsI GCATC 2 cut(s) 355, 367
BmuI ACTGGG 1 cut(s) 370
BpmI CTGGAG 2 cut(s) 389, 594
Bpu10I CCTNAGC 3 cut(s) 151, 264, 381
Bpu1102I GCTNAGC 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 83
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 3 cut(s) 204, 467, 531
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 2 cut(s) 217, 259
Bse1I ACTGG 3 cut(s) 365, 426, 577
Bse3DI GCAATG 1 cut(s) 725
BseBI CCWGG 3 cut(s) 407, 733, 750
BseDI CCNNGG 3 cut(s) 204, 467, 531
BseGI GGATG 4 cut(s) 370, 673, 700, 745
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 725
BseMII CTCAG 5 cut(s) 130, 165, 255, 372, 498
BseNI ACTGG 3 cut(s) 365, 426, 577
BseRI GAGGAG 8 cut(s) 49, 52, 55, 58, 61, 281, 284, 406
BseXI GCAGC 4 cut(s) 160, 163, 166, 514
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 3 cut(s) 227, 259, 582
BsiHKAI GWGCWC 1 cut(s) 242
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 2 cut(s) 218, 260
BslFI GGGAC 3 cut(s) 194, 331, 338
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 3 cut(s) 113, 692, 737
BsmFI GGGAC 3 cut(s) 194, 331, 338
BsnI GGCC 3 cut(s) 227, 259, 582
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 204, 242
Bsp143I GATC 2 cut(s) 589, 651
Bsp1720I GCTNAGC 1 cut(s) 507
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 3 cut(s) 224, 638, 655
BspANI GGCC 3 cut(s) 227, 259, 582
BspCNI CTCAG 5 cut(s) 129, 164, 256, 373, 499
BspLI GGNNCC 2 cut(s) 210, 404
BspPI GGATC 1 cut(s) 646
BsrDI GCAATG 1 cut(s) 725
BsrFI RCCGGY 2 cut(s) 217, 259
BsrI ACTGG 3 cut(s) 365, 426, 577
BssAI RCCGGY 2 cut(s) 217, 259
BssECI CCNNGG 3 cut(s) 204, 467, 531
BssMI GATC 2 cut(s) 589, 651
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 3 cut(s) 204, 467, 531
Bst2UI CCWGG 3 cut(s) 407, 733, 750
Bst4CI ACNGT 1 cut(s) 624
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 300
BstAPI GCANNNNNTGC 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 261, 726
BstDEI CTNAG 5 cut(s) 116, 151, 264, 381, 507
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 4 cut(s) 370, 673, 700, 745
BstKTI GATC 2 cut(s) 592, 654
BstMAI GTCTC 3 cut(s) 113, 692, 737
BstMBI GATC 2 cut(s) 589, 651
BstMWI GCNNNNNNNGC 5 cut(s) 173, 179, 185, 194, 260
BstNI CCWGG 3 cut(s) 407, 733, 750
BstSCI CCNGG 3 cut(s) 405, 731, 748
BstV1I GCAGC 4 cut(s) 160, 163, 166, 514
BstXI CCANNNNNNTGG 1 cut(s) 190
BsuRI GGCC 3 cut(s) 227, 259, 582
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 4 cut(s) 370, 673, 700, 745
BtsIMutI CAGTG 1 cut(s) 553
Cac8I GCNNGC 2 cut(s) 261, 726
CaiI CAGNNNCTG 1 cut(s) 173
Cfr10I RCCGGY 2 cut(s) 217, 259
Cfr13I GGNCC 3 cut(s) 208, 257, 580
CseI GACGC 1 cut(s) 308
Csp6I GTAC 2 cut(s) 464, 644
CviAII CATG 3 cut(s) 13, 477, 532
CviQI GTAC 2 cut(s) 464, 644
DdeI CTNAG 5 cut(s) 116, 151, 264, 381, 507
DpnI GATC 2 cut(s) 591, 653
DpnII GATC 2 cut(s) 589, 651
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Ecl136II GAGCTC 1 cut(s) 240
Eco130I CCWWGG 3 cut(s) 204, 467, 531
Eco24I GRGCYC 2 cut(s) 204, 242
Eco47I GGWCC 1 cut(s) 208
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 2 cut(s) 522, 529
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 240
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 3 cut(s) 405, 731, 748
EcoT14I CCWWGG 3 cut(s) 204, 467, 531
EcoT38I GRGCYC 2 cut(s) 204, 242
ErhI CCWWGG 3 cut(s) 204, 467, 531
FaeI CATG 3 cut(s) 16, 480, 535
FaiI YATR 5 cut(s) 14, 76, 443, 478, 533
FaqI GGGAC 3 cut(s) 194, 331, 338
FatI CATG 3 cut(s) 12, 476, 531
FauI CCCGC 1 cut(s) 631
FbaI TGATCA 1 cut(s) 589
Fnu4HI GCNGC 5 cut(s) 174, 177, 180, 225, 528
FokI GGATG 4 cut(s) 377, 680, 707, 752
FriOI GRGCYC 2 cut(s) 204, 242
Fsp4HI GCNGC 5 cut(s) 174, 177, 180, 225, 528
FspBI CTAG 2 cut(s) 387, 584
GluI GCNGC 5 cut(s) 174, 177, 180, 225, 528
GsaI CCCAGC 1 cut(s) 258
GsuI CTGGAG 2 cut(s) 389, 594
HaeIII GGCC 3 cut(s) 227, 259, 582
HapII CCGG 2 cut(s) 218, 260
HgaI GACGC 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 3 cut(s) 16, 480, 535
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HinfI GANTC 2 cut(s) 419, 745
HpaII CCGG 2 cut(s) 218, 260
HphI GGTGA 3 cut(s) 85, 130, 286
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 2 cut(s) 119, 651
Hpy8I GTNNAC 1 cut(s) 33
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 1 cut(s) 624
HpyCH4V TGCA 2 cut(s) 167, 612
HpyF10VI GCNNNNNNNGC 5 cut(s) 173, 179, 185, 194, 260
HpyF3I CTNAG 5 cut(s) 116, 151, 264, 381, 507
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 3 cut(s) 16, 480, 535
KroI GCCGGC 1 cut(s) 259
KroNI GCCGGC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 2 cut(s) 589, 651
LmnI GCTCC 2 cut(s) 170, 408
Lsp1109I GCAGC 4 cut(s) 160, 163, 166, 514
LweI GCATC 2 cut(s) 355, 367
MaeI CTAG 2 cut(s) 387, 584
MalI GATC 2 cut(s) 591, 653
MboI GATC 2 cut(s) 589, 651
MboII GAAGA 2 cut(s) 127, 535
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 204, 242
MluCI AATT 2 cut(s) 459, 601
MlyI GAGTC 1 cut(s) 739
MroNI GCCGGC 1 cut(s) 259
MslI CAYNNNNRTG 2 cut(s) 11, 188
MspA1I CMGCKG 3 cut(s) 155, 188, 254
MspI CCGG 2 cut(s) 218, 260
MspR9I CCNGG 3 cut(s) 407, 733, 750
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 3 cut(s) 407, 733, 750
MwoI GCNNNNNNNGC 5 cut(s) 173, 179, 185, 194, 260
NaeI GCCGGC 1 cut(s) 261
NcoI CCATGG 1 cut(s) 531
NdeII GATC 2 cut(s) 589, 651
NgoMIV GCCGGC 1 cut(s) 259
NlaIII CATG 3 cut(s) 16, 480, 535
NlaIV GGNNCC 2 cut(s) 210, 404
OliI CACNNNNGTG 1 cut(s) 188
PdiI GCCGGC 1 cut(s) 261
PfeI GAWTC 1 cut(s) 419
PfoI TCCNGGA 1 cut(s) 405
PkrI GCNGC 5 cut(s) 175, 178, 181, 226, 529
PleI GAGTC 1 cut(s) 739
PpsI GAGTC 1 cut(s) 739
PpuMI RGGWCCY 1 cut(s) 208
Psp124BI GAGCTC 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 208
Psp6I CCWGG 3 cut(s) 405, 731, 748
PspFI CCCAGC 1 cut(s) 254
PspGI CCWGG 3 cut(s) 405, 731, 748
PspN4I GGNNCC 2 cut(s) 210, 404
PspPI GGNCC 3 cut(s) 208, 257, 580
PspPPI RGGWCCY 1 cut(s) 208
PstNI CAGNNNCTG 1 cut(s) 173
PvuII CAGCTG 3 cut(s) 155, 188, 254
RsaI GTAC 2 cut(s) 465, 645
RsaNI GTAC 2 cut(s) 464, 644
RseI CAYNNNNRTG 2 cut(s) 11, 188
SacI GAGCTC 1 cut(s) 242
SatI GCNGC 5 cut(s) 174, 177, 180, 225, 528
Sau3AI GATC 2 cut(s) 589, 651
Sau96I GGNCC 3 cut(s) 208, 257, 580
ScaI AGTACT 1 cut(s) 645
SchI GAGTC 1 cut(s) 739
ScrFI CCNGG 3 cut(s) 407, 733, 750
SduI GDGCHC 2 cut(s) 204, 242
SfaNI GCATC 2 cut(s) 355, 367
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 11, 188
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 2 cut(s) 459, 601
SsiI CCGC 3 cut(s) 224, 638, 655
SspMI CTAG 2 cut(s) 387, 584
SstI GAGCTC 1 cut(s) 242
StyD4I CCNGG 3 cut(s) 405, 731, 748
StyI CCWWGG 3 cut(s) 204, 467, 531
TaaI ACNGT 1 cut(s) 624
TaqI TCGA 1 cut(s) 450
TasI AATT 2 cut(s) 459, 601
TatI WGTACW 1 cut(s) 643
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 419
TscAI CASTG 1 cut(s) 553
TseI GCWGC 4 cut(s) 173, 176, 179, 527
TspGWI ACGGA 1 cut(s) 18
TspRI CASTG 1 cut(s) 553
VpaK11BI GGWCC 1 cut(s) 208
XspI CTAG 2 cut(s) 387, 584
ZrmI AGTACT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.