RchiOBHm_Chr2g0157001

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017390
Physical Location & Seq
Reverse (-)
73494221 .. 73494904
684 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52576

Sequence Viewer

Length: 621 bp
ATGCCTAATCTAGGTGGTTTTGTCACTGAATTCGGATGGGATGGAAAGCATGCTGGGATCATCATACTTGTATCTCAGTTGGTAACGACTGATGATAATAGTCCCTTTATTTTAAATTCGGACCAACTTGTATGCTCAATTTTACCCGAATCACCTGCTCCGAAATCCGTCACATTCTCACCCGGTGGGCTATTGTTCAAACCCGGAGGAACTGCCAATAAAGTTGTTAACTGCGGCAACAACGTCGTGATCACTGCGTCTAGGATGATCGACTTCACCAAAGGACAGGTTGACTATATACTAGGAAGCAACCCATTGGGAATGTCATACATGGTGGGATATGGTTCAAAGTTTCCAAAGAGAATACACCACCGCGCTTCGGTGCTACCTTCTATGGATCAGCAGCCGGGCAACATTGAATGTCACGCAGGAACGCCATATTTCGAGACTCAAGCTCCAAATCAGAACTTGCTAATAGGCGCGATTGTGGGAGGACCTGCTGAGAACGATTCTTTCGAAGACTCTCGGTACAATGTGTACAATGTACACAGTCGGAGCCAAACACATACATCAATGCACCTTTTGTTGATGTTTTGGCTTACCTTAAGTCCATATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

206

Amino Acids

22.44

Weight (kDa)

6.12

Isoelectric Point (pI)

57.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 9 - 178 3.2e-34 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017390)

Species Orthologous Gene IDs
malus_domestica MD00G1133600.v1.1
pyrus_communis pycom13g01910
rosa_chinensis RchiOBHm_Chr1g0374621 RchiOBHm_Chr2g0157001
rosa_laevigata RLG00000020957
rosa_multiflora Rmu_co8499365.1_g000001
rosa_rugosa Rorug01G0059700 Rorug07G0215400
rosa_samantha Rh1AG395300 Rh1CG373200 Rh1DG390700 Rh2AG523300 Rh2BG536500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 163
Acc36I ACCTGC 2 cut(s) 163, 505
AccII CGCG 2 cut(s) 375, 482
AciI CCGC 2 cut(s) 234, 373
AclWI GGATC 2 cut(s) 65, 405
AcsI RAATTY 2 cut(s) 29, 115
AdeI CACNNNGTG 1 cut(s) 185
AfaI GTAC 3 cut(s) 530, 539, 546
AfiI CCNNNNNNNGG 2 cut(s) 11, 379
AflII CTTAAG 1 cut(s) 604
AgsI TTSAA 3 cut(s) 199, 348, 419
AluBI AGCT 1 cut(s) 455
AluI AGCT 1 cut(s) 455
Alw26I GTCTC 1 cut(s) 440
AlwI GGATC 2 cut(s) 65, 405
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 2 cut(s) 29, 115
AspLEI GCGC 2 cut(s) 377, 482
AspS9I GGNCC 2 cut(s) 121, 494
AsuC2I CCSGG 3 cut(s) 183, 204, 408
AsuHPI GGTGA 3 cut(s) 144, 171, 268
AsuII TTCGAA 1 cut(s) 516
AvaII GGWCC 2 cut(s) 121, 494
BbsI GAAGAC 1 cut(s) 525
BbvI GCAGC 1 cut(s) 415
BccI CCATC 2 cut(s) 30, 35
BcgI CGANNNNNNTGC 4 cut(s) 137, 171, 226, 260
BclI TGATCA 1 cut(s) 249
BcnI CCSGG 3 cut(s) 183, 204, 408
BcoDI GTCTC 1 cut(s) 440
BfaI CTAG 3 cut(s) 11, 261, 302
BfrI CTTAAG 1 cut(s) 604
BfuAI ACCTGC 2 cut(s) 163, 505
BisI GCNGC 2 cut(s) 235, 404
BlsI GCNGC 2 cut(s) 236, 405
Bme1390I CCNGG 3 cut(s) 183, 204, 408
Bme18I GGWCC 2 cut(s) 121, 494
BmgT120I GGNCC 2 cut(s) 121, 494
BmiI GGNNCC 1 cut(s) 557
BmrFI CCNGG 3 cut(s) 183, 204, 408
BpiI GAAGAC 1 cut(s) 525
Bpu14I TTCGAA 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 435
BpuMI CCSGG 3 cut(s) 183, 204, 408
BsaXI ACNNNNNCTCC 2 cut(s) 439, 469
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 379
BseGI GGATG 3 cut(s) 41, 46, 270
BseLI CCNNNNNNNGG 2 cut(s) 11, 379
BseMII CTCAG 2 cut(s) 89, 492
BseXI GCAGC 1 cut(s) 415
BseYI CCCAGC 1 cut(s) 53
Bsh1236I CGCG 2 cut(s) 375, 482
BsiSI CCGG 3 cut(s) 183, 204, 407
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 2 cut(s) 11, 379
BsmAI GTCTC 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 87
Bsp119I TTCGAA 1 cut(s) 516
Bsp1407I TGTACA 2 cut(s) 537, 544
Bsp143I GATC 4 cut(s) 57, 249, 267, 397
BspACI CCGC 2 cut(s) 234, 373
BspCNI CTCAG 2 cut(s) 88, 493
BspFNI CGCG 2 cut(s) 375, 482
BspLI GGNNCC 1 cut(s) 557
BspMI ACCTGC 2 cut(s) 163, 505
BspPI GGATC 2 cut(s) 65, 405
BspT104I TTCGAA 1 cut(s) 516
BspTI CTTAAG 1 cut(s) 604
BsrGI TGTACA 2 cut(s) 537, 544
BssMI GATC 4 cut(s) 57, 249, 267, 397
Bst4CI ACNGT 1 cut(s) 551
BstAFI CTTAAG 1 cut(s) 604
BstAUI TGTACA 2 cut(s) 537, 544
BstBI TTCGAA 1 cut(s) 516
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 2 cut(s) 75, 501
BstENI CCTNNNNNAGG 1 cut(s) 9
BstF5I GGATG 3 cut(s) 41, 46, 270
BstFNI CGCG 2 cut(s) 375, 482
BstHHI GCGC 2 cut(s) 377, 482
BstKTI GATC 4 cut(s) 60, 252, 270, 400
BstMAI GTCTC 1 cut(s) 440
BstMBI GATC 4 cut(s) 57, 249, 267, 397
BstNSI RCATGY 1 cut(s) 53
BstSCI CCNGG 3 cut(s) 181, 202, 406
BstUI CGCG 2 cut(s) 375, 482
BstV1I GCAGC 1 cut(s) 415
BstV2I GAAGAC 1 cut(s) 525
BtsCI GGATG 3 cut(s) 41, 46, 270
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 24, 252
BveI ACCTGC 2 cut(s) 163, 505
Cac8I GCNNGC 1 cut(s) 51
CfoI GCGC 2 cut(s) 377, 482
Cfr13I GGNCC 2 cut(s) 121, 494
CseI GACGC 1 cut(s) 246
Csp6I GTAC 3 cut(s) 529, 538, 545
CviAII CATG 2 cut(s) 50, 331
CviJI RGCY 5 cut(s) 190, 406, 455, 558, 598
CviKI_1 RGCY 5 cut(s) 190, 406, 455, 558, 598
CviQI GTAC 3 cut(s) 529, 538, 545
DdeI CTNAG 2 cut(s) 75, 501
DpnI GATC 4 cut(s) 59, 251, 269, 399
DpnII GATC 4 cut(s) 57, 249, 267, 397
DraI TTTAAA 1 cut(s) 114
DraIII CACNNNGTG 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 121, 494
EcoNI CCTNNNNNAGG 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 29
FaeI CATG 2 cut(s) 53, 334
FaqI GGGAC 1 cut(s) 87
FatI CATG 2 cut(s) 49, 330
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 2 cut(s) 235, 404
FokI GGATG 3 cut(s) 48, 53, 277
Fsp4HI GCNGC 2 cut(s) 235, 404
FspBI CTAG 3 cut(s) 11, 261, 302
GlaI GCGC 2 cut(s) 376, 481
GluI GCNGC 2 cut(s) 235, 404
GsaI CCCAGC 1 cut(s) 57
HapII CCGG 3 cut(s) 183, 204, 407
HgaI GACGC 1 cut(s) 246
HhaI GCGC 2 cut(s) 377, 482
Hin1II CATG 2 cut(s) 53, 334
Hin6I GCGC 2 cut(s) 375, 480
HinP1I GCGC 2 cut(s) 375, 480
HincII GTYRAC 2 cut(s) 229, 292
HindII GTYRAC 2 cut(s) 229, 292
HinfI GANTC 4 cut(s) 149, 448, 509, 521
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 3 cut(s) 183, 204, 407
HphI GGTGA 3 cut(s) 144, 171, 268
Hpy166II GTNNAC 4 cut(s) 229, 292, 538, 547
Hpy188I TCNGA 6 cut(s) 35, 121, 162, 465, 555, 620
Hpy188III TCNNGA 2 cut(s) 247, 445
Hpy8I GTNNAC 4 cut(s) 229, 292, 538, 547
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 1 cut(s) 399
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 1 cut(s) 577
HpyF3I CTNAG 2 cut(s) 75, 501
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 2 cut(s) 53, 334
HspAI GCGC 2 cut(s) 375, 480
Ksp22I TGATCA 1 cut(s) 249
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 4 cut(s) 57, 249, 267, 397
LmnI GCTCC 3 cut(s) 163, 460, 555
LpnPI CCDG 8 cut(s) 39, 168, 196, 217, 272, 414, 420, 510
Lsp1109I GCAGC 1 cut(s) 415
MaeI CTAG 3 cut(s) 11, 261, 302
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 4 cut(s) 22, 82, 169, 422
MalI GATC 4 cut(s) 59, 251, 269, 399
MboI GATC 4 cut(s) 57, 249, 267, 397
MboII GAAGA 1 cut(s) 530
MluCI AATT 3 cut(s) 29, 115, 138
MlyI GAGTC 2 cut(s) 442, 515
MmeI TCCRAC 1 cut(s) 533
MnlI CCTC 2 cut(s) 200, 485
MseI TTAA 3 cut(s) 113, 228, 605
MspCI CTTAAG 1 cut(s) 604
MspI CCGG 3 cut(s) 183, 204, 407
MspR9I CCNGG 3 cut(s) 183, 204, 408
MvnI CGCG 2 cut(s) 375, 482
NciI CCSGG 3 cut(s) 183, 204, 408
NdeII GATC 4 cut(s) 57, 249, 267, 397
NlaIII CATG 2 cut(s) 53, 334
NlaIV GGNNCC 1 cut(s) 557
NmuCI GTSAC 3 cut(s) 22, 169, 422
NspI RCATGY 1 cut(s) 53
NspV TTCGAA 1 cut(s) 516
PaeI GCATGC 1 cut(s) 53
PaqCI CACCTGC 1 cut(s) 163
PcsI WCGNNNNNNNCGW 1 cut(s) 513
PfeI GAWTC 2 cut(s) 149, 509
PkrI GCNGC 2 cut(s) 236, 405
PleI GAGTC 2 cut(s) 442, 515
PpsI GAGTC 2 cut(s) 442, 515
PpuMI RGGWCCY 1 cut(s) 494
Psp5II RGGWCCY 1 cut(s) 494
PspFI CCCAGC 1 cut(s) 53
PspN4I GGNNCC 1 cut(s) 557
PspPI GGNCC 2 cut(s) 121, 494
PspPPI RGGWCCY 1 cut(s) 494
RsaI GTAC 3 cut(s) 530, 539, 546
RsaNI GTAC 3 cut(s) 529, 538, 545
SaqAI TTAA 3 cut(s) 113, 228, 605
SatI GCNGC 2 cut(s) 235, 404
Sau3AI GATC 4 cut(s) 57, 249, 267, 397
Sau96I GGNCC 2 cut(s) 121, 494
SchI GAGTC 2 cut(s) 442, 515
ScrFI CCNGG 3 cut(s) 183, 204, 408
SetI ASST 9 cut(s) 16, 157, 246, 291, 391, 457, 499, 582, 605
SfuI TTCGAA 1 cut(s) 516
SinI GGWCC 2 cut(s) 121, 494
SmlI CTYRAG 2 cut(s) 450, 604
SmoI CTYRAG 2 cut(s) 450, 604
SphI GCATGC 1 cut(s) 53
Sse9I AATT 3 cut(s) 29, 115, 138
SsiI CCGC 2 cut(s) 234, 373
SspMI CTAG 3 cut(s) 11, 261, 302
StyD4I CCNGG 3 cut(s) 181, 202, 406
TaaI ACNGT 1 cut(s) 551
TaiI ACGT 1 cut(s) 246
TaqI TCGA 3 cut(s) 270, 444, 516
TasI AATT 3 cut(s) 29, 115, 138
TatI WGTACW 2 cut(s) 537, 544
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 2 cut(s) 149, 509
Tru1I TTAA 3 cut(s) 113, 228, 605
Tru9I TTAA 3 cut(s) 113, 228, 605
TscAI CASTG 2 cut(s) 31, 259
TseFI GTSAC 3 cut(s) 22, 169, 422
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 3 cut(s) 22, 169, 422
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 2 cut(s) 31, 259
Vha464I CTTAAG 1 cut(s) 604
VpaK11BI GGWCC 2 cut(s) 121, 494
XagI CCTNNNNNAGG 1 cut(s) 9
XapI RAATTY 2 cut(s) 29, 115
XceI RCATGY 1 cut(s) 53
XspI CTAG 3 cut(s) 11, 261, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.