RLG00000020957

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017390
Physical Location & Seq
Reverse (-)
70483731 .. 70485602
1872 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020957

Sequence Viewer

Length: 600 bp
ATGCCTAATCTAGGTGGTTCTGTCACTGAATTCGGATGGGATGGAAAGCATGCTGGGATCATCATACTTGTATCTCAGTTGGTAACGACTGATGATAATAGTCCCTTTATTTCAAATTCGGACCAACTTCTGAAAGCTGCCAATAAAGTTGTTAACTGCGGCAACAACGTCGTGATCACTGCGTCTAGGATGATCGACTTCACCAAAGGACAGGTTGACTATATACTAGGAAGCAACCCATTGGGAATGTCATACATGGTGGGATATGGTTCAAAGTTTCCAAAGAGAATACACCACCGCTCTTCGGTGCTACCTTCTATGGATCAGCAGCCGGGCAACATTGAATGTCACGCAGGAACGCCATATTTCGAGACTCAAGCTCCAAATCAGAACTTGCTAATAGGCGCGATTGTGGGAGGACCTGCTGAGAACGATTCTTTCGAAGACTCTCGGGAGAAGGCAGCGATGAGACGCCGTTGGCTCCTCAGTGATCAGTGGGAGAGTCCTTCGTCTTCTCCGGATTATTCACAAGTTAGTATCAAACACTTGGGTGCATACACTGTAGTTGCAGTGTTCTTTTTGGATATCTATTGGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

200

Amino Acids

21.83

Weight (kDa)

5.67

Isoelectric Point (pI)

57.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 46 - 154 2e-28 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017390)

Species Orthologous Gene IDs
malus_domestica MD00G1133600.v1.1
pyrus_communis pycom13g01910
rosa_chinensis RchiOBHm_Chr1g0374621 RchiOBHm_Chr2g0157001
rosa_laevigata RLG00000020957
rosa_multiflora Rmu_co8499365.1_g000001
rosa_rugosa Rorug01G0059700 Rorug07G0215400
rosa_samantha Rh1AG395300 Rh1CG373200 Rh1DG390700 Rh2AG523300 Rh2BG536500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 430
AccBSI CCGCTC 1 cut(s) 300
AccII CGCG 1 cut(s) 407
AccIII TCCGGA 1 cut(s) 517
AciI CCGC 2 cut(s) 159, 298
AclWI GGATC 2 cut(s) 65, 330
AcsI RAATTY 2 cut(s) 29, 115
AcyI GRCGYC 1 cut(s) 472
AfiI CCNNNNNNNGG 2 cut(s) 11, 304
AgsI TTSAA 3 cut(s) 114, 273, 344
AleI CACNNNNGTG 1 cut(s) 549
AluBI AGCT 2 cut(s) 137, 380
AluI AGCT 2 cut(s) 137, 380
Alw26I GTCTC 2 cut(s) 365, 463
AlwI GGATC 2 cut(s) 65, 330
Ama87I CYCGRG 1 cut(s) 450
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 1 cut(s) 594
ApeKI GCWGC 3 cut(s) 137, 328, 461
ApoI RAATTY 2 cut(s) 29, 115
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 3 cut(s) 121, 419, 594
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 1 cut(s) 193
AsuII TTCGAA 1 cut(s) 441
AvaI CYCGRG 1 cut(s) 450
AvaII GGWCC 2 cut(s) 121, 419
BbsI GAAGAC 2 cut(s) 450, 504
BbvI GCAGC 3 cut(s) 124, 340, 473
BccI CCATC 2 cut(s) 30, 35
BceAI ACGGC 1 cut(s) 459
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BclI TGATCA 2 cut(s) 174, 490
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 2 cut(s) 365, 463
BfaI CTAG 3 cut(s) 11, 186, 227
BfmI CTRYAG 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 430
BisI GCNGC 4 cut(s) 138, 160, 329, 462
BlsI GCNGC 4 cut(s) 139, 161, 330, 463
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 2 cut(s) 121, 419
BmeT110I CYCGRG 1 cut(s) 450
BmgT120I GGNCC 3 cut(s) 121, 419, 594
BmiI GGNNCC 1 cut(s) 482
BmrFI CCNGG 1 cut(s) 333
BpiI GAAGAC 2 cut(s) 450, 504
Bpu14I TTCGAA 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 472
BsaWI WCCGGW 1 cut(s) 517
BsaXI ACNNNNNCTCC 2 cut(s) 364, 394
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 304
BseAI TCCGGA 1 cut(s) 517
BseGI GGATG 3 cut(s) 41, 46, 195
BseLI CCNNNNNNNGG 2 cut(s) 11, 304
BseMII CTCAG 3 cut(s) 89, 417, 499
BseRI GAGGAG 1 cut(s) 473
BseXI GCAGC 3 cut(s) 124, 340, 473
BseYI CCCAGC 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 407
BshFI GGCC 1 cut(s) 596
BsiHKCI CYCGRG 1 cut(s) 450
BsiSI CCGG 2 cut(s) 332, 518
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 2 cut(s) 11, 304
BsmAI GTCTC 2 cut(s) 365, 463
BsmBI CGTCTC 1 cut(s) 463
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 1 cut(s) 596
BsoBI CYCGRG 1 cut(s) 450
Bsp119I TTCGAA 1 cut(s) 441
Bsp13I TCCGGA 1 cut(s) 517
Bsp143I GATC 5 cut(s) 57, 174, 192, 322, 490
BspACI CCGC 2 cut(s) 159, 298
BspANI GGCC 1 cut(s) 596
BspCNI CTCAG 3 cut(s) 88, 418, 498
BspEI TCCGGA 1 cut(s) 517
BspFNI CGCG 1 cut(s) 407
BspLI GGNNCC 1 cut(s) 482
BspMI ACCTGC 1 cut(s) 430
BspPI GGATC 2 cut(s) 65, 330
BspQI GCTCTTC 1 cut(s) 307
BspT104I TTCGAA 1 cut(s) 441
BsrBI CCGCTC 1 cut(s) 300
BssMI GATC 5 cut(s) 57, 174, 192, 322, 490
BssNI GRCGYC 1 cut(s) 472
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 1 cut(s) 307
BstACI GRCGYC 1 cut(s) 472
BstBI TTCGAA 1 cut(s) 441
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 3 cut(s) 75, 426, 485
BstENI CCTNNNNNAGG 1 cut(s) 9
BstF5I GGATG 3 cut(s) 41, 46, 195
BstFNI CGCG 1 cut(s) 407
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 5 cut(s) 60, 177, 195, 325, 493
BstMAI GTCTC 2 cut(s) 365, 463
BstMBI GATC 5 cut(s) 57, 174, 192, 322, 490
BstNSI RCATGY 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 331
BstSFI CTRYAG 1 cut(s) 561
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 3 cut(s) 124, 340, 473
BstV2I GAAGAC 2 cut(s) 450, 504
BsuRI GGCC 1 cut(s) 596
BtgZI GCGATG 1 cut(s) 479
BtsCI GGATG 3 cut(s) 41, 46, 195
BtsI GCAGTG 2 cut(s) 177, 576
BtsIMutI CAGTG 6 cut(s) 24, 177, 493, 500, 558, 576
BveI ACCTGC 1 cut(s) 430
Cac8I GCNNGC 1 cut(s) 51
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 3 cut(s) 121, 419, 594
CseI GACGC 2 cut(s) 171, 480
CviAII CATG 2 cut(s) 50, 256
CviJI RGCY 5 cut(s) 137, 331, 380, 481, 596
CviKI_1 RGCY 5 cut(s) 137, 331, 380, 481, 596
DdeI CTNAG 3 cut(s) 75, 426, 485
DpnI GATC 5 cut(s) 59, 176, 194, 324, 492
DpnII GATC 5 cut(s) 57, 174, 192, 322, 490
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 121, 419
Eco88I CYCGRG 1 cut(s) 450
EcoNI CCTNNNNNAGG 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 419
EcoRI GAATTC 1 cut(s) 29
EcoRV GATATC 1 cut(s) 586
Esp3I CGTCTC 1 cut(s) 463
FaeI CATG 2 cut(s) 53, 259
FaqI GGGAC 1 cut(s) 87
FatI CATG 2 cut(s) 49, 255
FbaI TGATCA 2 cut(s) 174, 490
Fnu4HI GCNGC 4 cut(s) 138, 160, 329, 462
FokI GGATG 3 cut(s) 48, 53, 202
Fsp4HI GCNGC 4 cut(s) 138, 160, 329, 462
FspBI CTAG 3 cut(s) 11, 186, 227
GlaI GCGC 1 cut(s) 406
GluI GCNGC 4 cut(s) 138, 160, 329, 462
GsaI CCCAGC 1 cut(s) 57
HaeIII GGCC 1 cut(s) 596
HapII CCGG 2 cut(s) 332, 518
HgaI GACGC 2 cut(s) 171, 480
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 1 cut(s) 472
Hin1II CATG 2 cut(s) 53, 259
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HincII GTYRAC 2 cut(s) 154, 217
HindII GTYRAC 2 cut(s) 154, 217
HinfI GANTC 4 cut(s) 373, 434, 446, 502
HpaI GTTAAC 1 cut(s) 154
HpaII CCGG 2 cut(s) 332, 518
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 2 cut(s) 154, 217
Hpy188I TCNGA 4 cut(s) 35, 121, 132, 390
Hpy188III TCNNGA 4 cut(s) 172, 370, 452, 518
Hpy8I GTNNAC 2 cut(s) 154, 217
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 3 cut(s) 324, 451, 516
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 2 cut(s) 554, 569
HpyF3I CTNAG 3 cut(s) 75, 426, 485
HpySE526I ACGT 1 cut(s) 168
Hsp92I GRCGYC 1 cut(s) 472
Hsp92II CATG 2 cut(s) 53, 259
HspAI GCGC 1 cut(s) 405
Kpn2I TCCGGA 1 cut(s) 517
Ksp22I TGATCA 2 cut(s) 174, 490
KspAI GTTAAC 1 cut(s) 154
Kzo9I GATC 5 cut(s) 57, 174, 192, 322, 490
LguI GCTCTTC 1 cut(s) 307
LmnI GCTCC 2 cut(s) 385, 486
LpnPI CCDG 6 cut(s) 39, 197, 339, 345, 435, 531
Lsp1109I GCAGC 3 cut(s) 124, 340, 473
MaeI CTAG 3 cut(s) 11, 186, 227
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 3 cut(s) 22, 82, 347
MalI GATC 5 cut(s) 59, 176, 194, 324, 492
MbiI CCGCTC 1 cut(s) 300
MboI GATC 5 cut(s) 57, 174, 192, 322, 490
MboII GAAGA 3 cut(s) 294, 455, 504
MluCI AATT 2 cut(s) 29, 115
MlyI GAGTC 3 cut(s) 367, 440, 511
MnlI CCTC 2 cut(s) 410, 494
MroI TCCGGA 1 cut(s) 517
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 549
MspI CCGG 2 cut(s) 332, 518
MspR9I CCNGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 407
NciI CCSGG 1 cut(s) 333
NdeII GATC 5 cut(s) 57, 174, 192, 322, 490
NlaIII CATG 2 cut(s) 53, 259
NlaIV GGNNCC 1 cut(s) 482
NmuCI GTSAC 2 cut(s) 22, 347
NspI RCATGY 1 cut(s) 53
NspV TTCGAA 1 cut(s) 441
OliI CACNNNNGTG 1 cut(s) 549
PaeI GCATGC 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PfeI GAWTC 1 cut(s) 434
PkrI GCNGC 4 cut(s) 139, 161, 330, 463
PleI GAGTC 3 cut(s) 367, 440, 510
PpsI GAGTC 3 cut(s) 367, 440, 510
PpuMI RGGWCCY 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 419
PspFI CCCAGC 1 cut(s) 53
PspN4I GGNNCC 1 cut(s) 482
PspPI GGNCC 3 cut(s) 121, 419, 594
PspPPI RGGWCCY 1 cut(s) 419
RseI CAYNNNNRTG 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 307
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 4 cut(s) 138, 160, 329, 462
Sau3AI GATC 5 cut(s) 57, 174, 192, 322, 490
Sau96I GGNCC 3 cut(s) 121, 419, 594
SchI GAGTC 3 cut(s) 367, 440, 511
ScrFI CCNGG 1 cut(s) 333
SetI ASST 7 cut(s) 16, 139, 171, 216, 316, 382, 424
SfcI CTRYAG 1 cut(s) 561
SfuI TTCGAA 1 cut(s) 441
SinI GGWCC 2 cut(s) 121, 419
SmiMI CAYNNNNRTG 1 cut(s) 549
SmlI CTYRAG 1 cut(s) 375
SmoI CTYRAG 1 cut(s) 375
SphI GCATGC 1 cut(s) 53
Sse9I AATT 2 cut(s) 29, 115
SsiI CCGC 2 cut(s) 159, 298
SspMI CTAG 3 cut(s) 11, 186, 227
StyD4I CCNGG 1 cut(s) 331
TaaI ACNGT 1 cut(s) 562
TaiI ACGT 1 cut(s) 171
TaqI TCGA 3 cut(s) 195, 369, 441
TasI AATT 2 cut(s) 29, 115
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 434
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 6 cut(s) 31, 184, 493, 500, 565, 576
TseFI GTSAC 2 cut(s) 22, 347
TseI GCWGC 3 cut(s) 137, 328, 461
Tsp45I GTSAC 2 cut(s) 22, 347
TspRI CASTG 6 cut(s) 31, 184, 493, 500, 565, 576
VpaK11BI GGWCC 2 cut(s) 121, 419
XagI CCTNNNNNAGG 1 cut(s) 9
XapI RAATTY 2 cut(s) 29, 115
XceI RCATGY 1 cut(s) 53
XspI CTAG 3 cut(s) 11, 186, 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.