MD00G1164900.v1.1
ERF Family

HhH-GPD superfamily base excision DNA repair protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007267
Physical Location & Seq
Forward (+)
37523950 .. 37528584
4635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1164900.v1.1.491

Sequence Viewer

Length: 2505 bp
ATGGAGGAAGGCGAGAGACCATTGTGCCCACAGCAGCAGCAGCAGCAGCCGATTAAGAAGAAGAAAAACCAGCCTCAGCCTCCGCCTCCACCTCCGCCTCCACCAGTAATTGAGACGCCGTCGTTGATTGGAGAAGATAGCGGAGGTAAGCGCAGGAAGAAGAAGAAGAAAGAGGAGGAGGGTTGTGCCAATTTCGCCAGTTTACCTGTCGCCGTTCCTGAAGAAGACAATAAGAGTAAGAATAGCGAGGGGAACAAGAAGAAGAGTAAGGGTAAGAGAAAGAACAATGGCAATTCAGTTGCAGATGACGCCGACTGCAAGAGAAGAAGAAAAACCCCCATACTTGTCGGCGACCGGGTGGTTTCTCCGTATTTTCAGAATCATCATGGAGGAAGAGGCGGCGGAGAAAAAGGTAAGACCTTTAGCAGCAATATTGTAAATATACAAATATGTGAAACTGAAATAAAATTCAAGAAACACAAACACAAGAAGAAAAAGGAACCAGTTAATTGTAATGCGGGTTTACTTGAGGCCGAGGCCAATCCCAATGCCAATGACAATGCCAATTCCAAGCCCACCAACAAAAGAAGAAGAAAACTCTCTGAATCCGATGATGGCCACCGGGTGGTTTCTCCGTATTTCCAGAATTTAGGAAAAGAAAAGGGTAACATCTTGCCAGCATCTGCCATAAGTTCAGAGTTGGTTTTTAGTAATGTGGTAGCTGAAACTGAAAGGTCATCTTATGGAAATCAAACCAAGAAAAACAAGAACTCGGAACAGGGAATTTGTAATGTGGATTCACTCGATGTTAATTCCAATGTCAATTCCGATCATAAAAGTAGATGGATAGTGCCTGTATGTGTTGGAGAGCGAGTGGTTTCTCCGTATTTTCTGAATCAACGAGGAGATATCAAAAAGGGTGAAATGTTAGAAACCTCTCTTGACAATTCGGGCATCAATGTAGTAGCTGAAATTGAAAAGGTTTCTCATGAACATAAAACCAAGAAAAAGGAACATGCAGGTTCCGATGGTATTGGTATCAAGAACACGAGGACAGGGGTAACACAACATGATAATGATAAAAGTAGTGACAGCTATGCTCCCAAGCTCGAGGAGATTATACTAAAAGCCATTCACAAAGATGAAAAAATAGAATCTGCAGGTGTAGCAACTGGTGAAGCAGAGATATTCGGTGATAATTGCATTGAAAAGAAGAAAAAGAAGAGGATTACGAAAGAGAAGAAGATGGAAAACCATGAAAGAAGCACTATCATGAAGGTGGTAAAGGAGAAGGAAAGGGATGTTAAAGAGAAGTCGGGGATCAATCACCAAATAAAGGCTAGTCAAAGTAATAATGTGATTTTGAGCCTTGAGGATGTGCTTGCACAGTTTGCATACAACGCTGGTGCTAGCATGAACAACACACGGATTGACAAATATGAAAAGATGGTCAGAAATGTGCAAACTTATTCTCAGATTAGTAGTGCAAAGAAACAGGTAGTGGAAGAAAAGGAGGAGAAAAAGTATCAAAATGAAAGTGAGACAAAACCTCCTTTCCCATTGAATAACATTACCAATTCTTCCATTCTAGTTAGCGAGAGTGGGAGGAGCACAACTGAGGAGCATGCCACTATGCCAAGCACCTGCAGAACCTTCGGAGATGAGAAAGTGAGTCCAAATAGTGCAGAAAATGGACAGGTTTCTTCCAAGAGAAGGAAGAATAATGAGAAGCACAAAAGAAGAGCACGTGTTGAAGTTCGAAAGGTTTCCCCTTACTTTCAAAAATCAGGAGTGGAAGAAGCAATGGTTAACTGGGGTGATGATACAAAAGCTAAGCCACCTAAACGCTGTTCAAAAACTTGTTCCACGTCTGTGAAGGTCTCTCCCTACTTTCAGAAAGAACACAAAGCAGACGGTAATGTGGTCGGACACTTGTTGGGGAGTAAAAAAAAGCGCAATAGATCTCCAGGAATCAAGACTGCTCTTTCTGCTTCCCAGAAGAAAGACGAAGCATACCTAAGACGAACTCCAGATAACACATGGGTTCCTCCTCGCTCTGAACATGGTCTCATCCAAGAGGATCATTATCATGATCCTTGGAGAGTACTGGTGATATGCATGCTCCTTAATCGCACAAGGGGATTGCAGGCAAAAAGAGTTATATCTGATCTCTTTACATTGTGTCCGGATGCAAAAGCTGCGACTGAGGTTGCTACAGAGGATATAGAAAAGACTATAAAAACTCTTGGTTTACATAAGAAGAGGGCATTGATGATACAGCATTTGTCTCGTGAGTATTTGGAGGAAAGCTGGACCCATGTTACTCAGTTGCATGGTGTTGGCAAGTATGCAGCTGACGCTTATGCAATATTTTGCACTGGTAGGTGGGAGCGAGTAAAACCTACAGACCACATGCTAAATTTCTACTGGGAATTTCTGCGCAAAACTGTACCGAGGGACGGTGTAAGAGAGAGTTTGGTTGGAGAGCAGATAGTTGCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000700 GO:0000785 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003696 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006220 GO:0006244 GO:0006259 GO:0006281 GO:0006284 GO:0006285 GO:0006304 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008263 GO:0009056 GO:0009058 GO:0009117 GO:0009166 GO:0009219 GO:0009223 GO:0009262 GO:0009264 GO:0009314 GO:0009394 GO:0009628 GO:0009987 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016798 GO:0016799 GO:0018130 GO:0019104 GO:0019438 GO:0019439 GO:0019637 GO:0019692 GO:0031974 GO:0031981 GO:0033554 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045008 GO:0046386 GO:0046434 GO:0046483 GO:0046700 GO:0050896 GO:0051716 GO:0055086 GO:0070013 GO:0071704 GO:0072527 GO:0072529 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901135 GO:1901136 GO:1901292 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1990837
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
HhH-GPD

Protein Analysis

835

Amino Acids

93.72

Weight (kDa)

9.49

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1151, 1652
Acc16I TGCGCA 1 cut(s) 2441
Acc36I ACCTGC 3 cut(s) 1010, 1151, 1652
AccB7I CCANNNNNTGG 1 cut(s) 625
AccIII TCCGGA 1 cut(s) 2185
AciI CCGC 6 cut(s) 83, 95, 141, 399, 402, 518
AclWI GGATC 3 cut(s) 1328, 2087, 2088
AcoI YGGCCR 1 cut(s) 616
AcsI RAATTY 5 cut(s) 467, 646, 783, 2419, 2432
AcuI CTGAAG 1 cut(s) 240
AcvI CACGTG 1 cut(s) 1748
AcyI GRCGYC 2 cut(s) 116, 309
AdeI CACNNNGTG 1 cut(s) 625
AfaI GTAC 2 cut(s) 2106, 2451
AfiI CCNNNNNNNGG 4 cut(s) 625, 1336, 1713, 2459
AflIII ACRYGT 1 cut(s) 1747
AgsI TTSAA 7 cut(s) 472, 977, 1208, 1564, 1754, 1781, 1854
AjiI CACGTC 1 cut(s) 1869
AjnI CCWGG 1 cut(s) 1966
AleI CACNNNNGTG 1 cut(s) 1871
AluBI AGCT 8 cut(s) 722, 968, 1095, 1108, 1832, 2198, 2310, 2354
AluI AGCT 8 cut(s) 722, 968, 1095, 1108, 1832, 2198, 2310, 2354
Alw21I GWGCWC 2 cut(s) 1613, 1747
Alw26I GTCTC 6 cut(s) 10, 107, 1535, 1885, 2072, 2292
AlwI GGATC 3 cut(s) 1328, 2087, 2088
AlwNI CAGNNNCTG 1 cut(s) 683
Ama87I CYCGRG 1 cut(s) 1109
Aor13HI TCCGGA 1 cut(s) 2185
AoxI GGCC 3 cut(s) 531, 537, 616
ApeKI GCWGC 8 cut(s) 34, 37, 40, 43, 46, 426, 2198, 2351
ApoI RAATTY 5 cut(s) 467, 646, 783, 2419, 2432
Asp700I GAANNNNTTC 1 cut(s) 1765
AspLEI GCGC 3 cut(s) 153, 1956, 2442
AspS9I GGNCC 1 cut(s) 2313
AsuC2I CCSGG 2 cut(s) 356, 623
AsuHPI GGTGA 6 cut(s) 932, 1187, 1205, 1319, 1829, 2122
AsuII TTCGAA 1 cut(s) 1759
AsuNHI GCTAGC 1 cut(s) 1409
AvaI CYCGRG 1 cut(s) 1109
AvaII GGWCC 1 cut(s) 2313
BaeGI GKGCMC 1 cut(s) 29
BalI TGGCCA 1 cut(s) 618
BauI CACGAG 2 cut(s) 1048, 2289
BbrPI CACGTG 1 cut(s) 1748
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 2 cut(s) 1613, 1747
BbvCI CCTCAGC 1 cut(s) 75
BbvI GCAGC 8 cut(s) 46, 49, 52, 55, 58, 438, 2185, 2363
BccI CCATC 5 cut(s) 608, 837, 1022, 1240, 1441
BceAI ACGGC 2 cut(s) 103, 197
BcgI CGANNNNNNTGC 4 cut(s) 1636, 1670, 2270, 2304
BciT130I CCWGG 1 cut(s) 1968
BcnI CCSGG 2 cut(s) 356, 623
BcoDI GTCTC 6 cut(s) 10, 107, 1535, 1885, 2072, 2292
BfaI CTAG 4 cut(s) 1341, 1410, 1589, 2499
BfmI CTRYAG 4 cut(s) 1158, 1645, 2214, 2403
BfuAI ACCTGC 3 cut(s) 1010, 1151, 1652
BglII AGATCT 1 cut(s) 1961
BisI GCNGC 9 cut(s) 35, 38, 41, 44, 47, 400, 427, 2199, 2352
BlpI GCTNAGC 1 cut(s) 1833
BlsI GCNGC 9 cut(s) 36, 39, 42, 45, 48, 401, 428, 2200, 2353
BmcAI AGTACT 1 cut(s) 2106
Bme1390I CCNGG 3 cut(s) 356, 623, 1968
Bme18I GGWCC 1 cut(s) 2313
BmeT110I CYCGRG 1 cut(s) 1109
BmgBI CACGTC 1 cut(s) 1869
BmgT120I GGNCC 1 cut(s) 2313
BmiI GGNNCC 4 cut(s) 501, 1024, 2046, 2315
BmrFI CCNGG 3 cut(s) 356, 623, 1968
BmrI ACTGGG 2 cut(s) 1822, 2437
BmsI GCATC 3 cut(s) 689, 963, 2179
BmtI GCTAGC 1 cut(s) 1413
BmuI ACTGGG 2 cut(s) 1822, 2437
BpiI GAAGAC 1 cut(s) 231
BpmI CTGGAG 2 cut(s) 1950, 2013
Bpu10I CCTNAGC 1 cut(s) 75
Bpu1102I GCTNAGC 1 cut(s) 1833
Bpu14I TTCGAA 1 cut(s) 1759
BpuEI CTTGAG 2 cut(s) 548, 1391
BpuMI CCSGG 2 cut(s) 356, 623
BsaAI YACGTR 1 cut(s) 1748
BsaBI GATNNNNATC 1 cut(s) 2085
BsaHI GRCGYC 2 cut(s) 116, 309
BsaI GGTCTC 3 cut(s) 10, 1885, 2072
BsaJI CCNNGG 3 cut(s) 534, 2096, 2453
BsaWI WCCGGW 1 cut(s) 2185
BsaXI ACNNNNNCTCC 6 cut(s) 858, 888, 1508, 1534, 1538, 1564
Bsc4I CCNNNNNNNGG 4 cut(s) 625, 1336, 1713, 2459
Bse1I ACTGG 8 cut(s) 104, 198, 503, 1177, 1817, 2112, 2383, 2432
Bse3DI GCAATG 1 cut(s) 1809
Bse8I GATNNNNATC 1 cut(s) 2085
BseAI TCCGGA 1 cut(s) 2185
BseBI CCWGG 1 cut(s) 1968
BseDI CCNNGG 3 cut(s) 534, 2096, 2453
BseGI GGATG 4 cut(s) 1306, 1381, 2070, 2194
BseJI GATNNNNATC 1 cut(s) 2085
BseLI CCNNNNNNNGG 4 cut(s) 625, 1336, 1713, 2459
BseMI GCAATG 1 cut(s) 1809
BseMII CTCAG 5 cut(s) 89, 1487, 1608, 2196, 2339
BseNI ACTGG 8 cut(s) 104, 198, 503, 1177, 1817, 2112, 2383, 2432
BseRI GAGGAG 8 cut(s) 188, 191, 918, 1127, 1529, 1621, 1634, 2040
BseSI GKGCMC 1 cut(s) 29
BseXI GCAGC 8 cut(s) 46, 49, 52, 55, 58, 438, 2185, 2363
BsgI GTGCAG 1 cut(s) 1704
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 3 cut(s) 533, 539, 618
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 2 cut(s) 1613, 1747
BsiHKCI CYCGRG 1 cut(s) 1109
BsiSI CCGG 3 cut(s) 355, 622, 2186
BslFI GGGAC 1 cut(s) 2471
BslI CCNNNNNNNGG 4 cut(s) 625, 1336, 1713, 2459
BsmAI GTCTC 6 cut(s) 10, 107, 1535, 1885, 2072, 2292
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 2471
BsnI GGCC 3 cut(s) 533, 539, 618
Bso31I GGTCTC 3 cut(s) 10, 1885, 2072
BsoBI CYCGRG 1 cut(s) 1109
Bsp119I TTCGAA 1 cut(s) 1759
Bsp1286I GDGCHC 3 cut(s) 29, 1613, 1747
Bsp13I TCCGGA 1 cut(s) 2185
Bsp143I GATC 6 cut(s) 829, 1320, 1961, 2080, 2092, 2167
Bsp1720I GCTNAGC 1 cut(s) 1833
BspACI CCGC 6 cut(s) 83, 95, 141, 399, 402, 518
BspANI GGCC 3 cut(s) 533, 539, 618
BspCNI CTCAG 5 cut(s) 88, 1486, 1609, 2197, 2338
BspEI TCCGGA 1 cut(s) 2185
BspHI TCATGA 3 cut(s) 988, 1272, 2089
BspLI GGNNCC 4 cut(s) 501, 1024, 2046, 2315
BspMAI CTGCAG 2 cut(s) 1162, 1649
BspMI ACCTGC 3 cut(s) 1010, 1151, 1652
BspOI GCTAGC 1 cut(s) 1413
BspPI GGATC 3 cut(s) 1328, 2087, 2088
BspQI GCTCTTC 1 cut(s) 1735
BspT104I TTCGAA 1 cut(s) 1759
BspTNI GGTCTC 3 cut(s) 10, 1885, 2072
BsrDI GCAATG 1 cut(s) 1809
BsrI ACTGG 8 cut(s) 104, 198, 503, 1177, 1817, 2112, 2383, 2432
BssECI CCNNGG 3 cut(s) 534, 2096, 2453
BssMI GATC 6 cut(s) 829, 1320, 1961, 2080, 2092, 2167
BssNI GRCGYC 2 cut(s) 116, 309
BssSI CACGAG 2 cut(s) 1048, 2289
BssT1I CCWWGG 1 cut(s) 2096
Bst2BI CACGAG 2 cut(s) 1048, 2289
Bst2UI CCWGG 1 cut(s) 1968
Bst4CI ACNGT 4 cut(s) 1389, 1916, 2449, 2462
Bst6I CTCTTC 5 cut(s) 257, 388, 1217, 1735, 2255
BstACI GRCGYC 2 cut(s) 116, 309
BstAPI GCANNNNNTGC 2 cut(s) 1391, 2198
BstBAI YACGTR 1 cut(s) 1748
BstBI TTCGAA 1 cut(s) 1759
BstC8I GCNNGC 6 cut(s) 678, 1383, 1411, 1626, 2120, 2148
BstDEI CTNAG 7 cut(s) 75, 1473, 1617, 1833, 2018, 2205, 2325
BstF5I GGATG 4 cut(s) 1306, 1381, 2070, 2194
BstHHI GCGC 3 cut(s) 153, 1956, 2442
BstKTI GATC 6 cut(s) 832, 1323, 1964, 2083, 2095, 2170
BstMAI GTCTC 6 cut(s) 10, 107, 1535, 1885, 2072, 2292
BstMBI GATC 6 cut(s) 829, 1320, 1961, 2080, 2092, 2167
BstMCI CGRYCG 1 cut(s) 355
BstNI CCWGG 1 cut(s) 1968
BstNSI RCATGY 4 cut(s) 1019, 1628, 2122, 2416
BstSCI CCNGG 3 cut(s) 354, 621, 1966
BstSFI CTRYAG 4 cut(s) 1158, 1645, 2214, 2403
BstSLI GKGCMC 1 cut(s) 29
BstV1I GCAGC 8 cut(s) 46, 49, 52, 55, 58, 438, 2185, 2363
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 1961
BstYI RGATCY 1 cut(s) 1961
BsuRI GGCC 3 cut(s) 533, 539, 618
BtrI CACGTC 1 cut(s) 1869
BtsCI GGATG 4 cut(s) 1306, 1381, 2070, 2194
BtsIMutI CAGTG 1 cut(s) 2376
BveI ACCTGC 3 cut(s) 1010, 1151, 1652
Cac8I GCNNGC 6 cut(s) 678, 1383, 1411, 1626, 2120, 2148
CaiI CAGNNNCTG 1 cut(s) 683
CciI TCATGA 3 cut(s) 988, 1272, 2089
CfoI GCGC 3 cut(s) 153, 1956, 2442
Cfr13I GGNCC 1 cut(s) 2313
CseI GACGC 3 cut(s) 124, 317, 2366
Csp6I GTAC 2 cut(s) 2105, 2450
CviQI GTAC 2 cut(s) 2105, 2450
DdeI CTNAG 7 cut(s) 75, 1473, 1617, 1833, 2018, 2205, 2325
DpnI GATC 6 cut(s) 831, 1322, 1963, 2082, 2094, 2169
DpnII GATC 6 cut(s) 829, 1320, 1961, 2080, 2092, 2167
DraIII CACNNNGTG 1 cut(s) 625
EaeI YGGCCR 1 cut(s) 616
Eam1104I CTCTTC 5 cut(s) 257, 388, 1217, 1735, 2255
EarI CTCTTC 5 cut(s) 257, 388, 1217, 1735, 2255
EciI GGCGGA 3 cut(s) 72, 84, 417
Eco130I CCWWGG 1 cut(s) 2096
Eco31I GGTCTC 3 cut(s) 10, 1885, 2072
Eco32I GATATC 1 cut(s) 910
Eco47I GGWCC 1 cut(s) 2313
Eco57I CTGAAG 1 cut(s) 240
Eco72I CACGTG 1 cut(s) 1748
Eco88I CYCGRG 1 cut(s) 1109
EcoRII CCWGG 1 cut(s) 1966
EcoRV GATATC 1 cut(s) 910
EcoT14I CCWWGG 1 cut(s) 2096
EcoT22I ATGCAT 1 cut(s) 2120
ErhI CCWWGG 1 cut(s) 2096
Esp3I CGTCTC 1 cut(s) 107
FalI AAGNNNNNCTT 2 cut(s) 724, 756
FaqI GGGAC 1 cut(s) 2471
FauI CCCGC 1 cut(s) 511
Fnu4HI GCNGC 9 cut(s) 35, 38, 41, 44, 47, 400, 427, 2199, 2352
FokI GGATG 4 cut(s) 1313, 1388, 2057, 2201
Fsp4HI GCNGC 9 cut(s) 35, 38, 41, 44, 47, 400, 427, 2199, 2352
FspBI CTAG 4 cut(s) 1341, 1410, 1589, 2499
FspI TGCGCA 1 cut(s) 2441
GlaI GCGC 3 cut(s) 152, 1955, 2441
GluI GCNGC 9 cut(s) 35, 38, 41, 44, 47, 400, 427, 2199, 2352
GsuI CTGGAG 2 cut(s) 1950, 2013
HaeIII GGCC 3 cut(s) 533, 539, 618
HapII CCGG 3 cut(s) 355, 622, 2186
HgaI GACGC 3 cut(s) 124, 317, 2366
HhaI GCGC 3 cut(s) 153, 1956, 2442
Hin1I GRCGYC 2 cut(s) 116, 309
Hin6I GCGC 3 cut(s) 151, 1954, 2440
HinP1I GCGC 3 cut(s) 151, 1954, 2440
HincII GTYRAC 1 cut(s) 1810
HindII GTYRAC 1 cut(s) 1810
HinfI GANTC 7 cut(s) 379, 605, 797, 895, 1154, 1672, 1971
HpaI GTTAAC 1 cut(s) 1810
HpaII CCGG 3 cut(s) 355, 622, 2186
HphI GGTGA 6 cut(s) 932, 1187, 1205, 1319, 1829, 2122
Hpy166II GTNNAC 4 cut(s) 203, 524, 1810, 2252
Hpy8I GTNNAC 4 cut(s) 203, 524, 1810, 2252
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 5 cut(s) 1270, 1285, 1663, 1707, 1870
HpyCH4III ACNGT 4 cut(s) 1389, 1916, 2449, 2462
HpyCH4IV ACGT 2 cut(s) 1747, 1868
HpyF3I CTNAG 7 cut(s) 75, 1473, 1617, 1833, 2018, 2205, 2325
HpySE526I ACGT 2 cut(s) 1747, 1868
Hsp92I GRCGYC 2 cut(s) 116, 309
HspAI GCGC 3 cut(s) 151, 1954, 2440
Kpn2I TCCGGA 1 cut(s) 2185
KspAI GTTAAC 1 cut(s) 1810
Kzo9I GATC 6 cut(s) 829, 1320, 1961, 2080, 2092, 2167
LguI GCTCTTC 1 cut(s) 1735
LmnI GCTCC 5 cut(s) 1105, 1608, 1621, 2127, 2389
Lsp1109I GCAGC 8 cut(s) 46, 49, 52, 55, 58, 438, 2185, 2363
LweI GCATC 3 cut(s) 689, 963, 2179
MaeI CTAG 4 cut(s) 1341, 1410, 1589, 2499
MaeII ACGT 2 cut(s) 1747, 1868
MaeIII GTNAC 4 cut(s) 665, 1060, 1088, 2320
MalI GATC 6 cut(s) 831, 1322, 1963, 2082, 2094, 2169
MboI GATC 6 cut(s) 829, 1320, 1961, 2080, 2092, 2167
MflI RGATCY 1 cut(s) 1961
MhlI GDGCHC 3 cut(s) 29, 1613, 1747
MlsI TGGCCA 1 cut(s) 618
MluNI TGGCCA 1 cut(s) 618
MlyI GAGTC 1 cut(s) 1681
MmeI TCCRAC 3 cut(s) 844, 1906, 2461
Mox20I TGGCCA 1 cut(s) 618
Mph1103I ATGCAT 1 cut(s) 2120
MroI TCCGGA 1 cut(s) 2185
MroXI GAANNNNTTC 1 cut(s) 1765
MscI TGGCCA 1 cut(s) 618
MseI TTAA 6 cut(s) 54, 507, 810, 1305, 1809, 2127
MslI CAYNNNNRTG 6 cut(s) 1074, 1140, 1271, 1277, 1871, 2088
Msp20I TGGCCA 1 cut(s) 618
MspA1I CMGCKG 1 cut(s) 2354
MspI CCGG 3 cut(s) 355, 622, 2186
MspR9I CCNGG 3 cut(s) 356, 623, 1968
MvaI CCWGG 1 cut(s) 1968
NciI CCSGG 2 cut(s) 356, 623
NdeII GATC 6 cut(s) 829, 1320, 1961, 2080, 2092, 2167
NheI GCTAGC 1 cut(s) 1409
NlaIV GGNNCC 4 cut(s) 501, 1024, 2046, 2315
NmeAIII GCCGAG 1 cut(s) 559
NmuCI GTSAC 1 cut(s) 1088
NsbI TGCGCA 1 cut(s) 2441
NsiI ATGCAT 1 cut(s) 2120
NspI RCATGY 4 cut(s) 1019, 1628, 2122, 2416
NspV TTCGAA 1 cut(s) 1759
OliI CACNNNNGTG 1 cut(s) 1871
PaeI GCATGC 2 cut(s) 1628, 2122
PaeR7I CTCGAG 1 cut(s) 1109
PagI TCATGA 3 cut(s) 988, 1272, 2089
PaqCI CACCTGC 2 cut(s) 1151, 1652
PciSI GCTCTTC 1 cut(s) 1735
PdmI GAANNNNTTC 1 cut(s) 1765
PfeI GAWTC 6 cut(s) 379, 605, 797, 895, 1154, 1971
PflFI GACNNNGTC 1 cut(s) 118
PflMI CCANNNNNTGG 1 cut(s) 625
PfoI TCCNGGA 1 cut(s) 1966
PkrI GCNGC 9 cut(s) 36, 39, 42, 45, 48, 401, 428, 2200, 2353
PleI GAGTC 1 cut(s) 1680
PmaCI CACGTG 1 cut(s) 1748
PmlI CACGTG 1 cut(s) 1748
PpsI GAGTC 1 cut(s) 1680
Ppu21I YACGTR 1 cut(s) 1748
Psp6I CCWGG 1 cut(s) 1966
PspCI CACGTG 1 cut(s) 1748
PspGI CCWGG 1 cut(s) 1966
PspN4I GGNNCC 4 cut(s) 501, 1024, 2046, 2315
PspPI GGNCC 1 cut(s) 2313
PspXI VCTCGAGB 1 cut(s) 1109
PstI CTGCAG 2 cut(s) 1162, 1649
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 1 cut(s) 1961
PsyI GACNNNGTC 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 2354
RsaI GTAC 2 cut(s) 2106, 2451
RsaNI GTAC 2 cut(s) 2105, 2450
RseI CAYNNNNRTG 6 cut(s) 1074, 1140, 1271, 1277, 1871, 2088
SapI GCTCTTC 1 cut(s) 1735
SaqAI TTAA 6 cut(s) 54, 507, 810, 1305, 1809, 2127
SatI GCNGC 9 cut(s) 35, 38, 41, 44, 47, 400, 427, 2199, 2352
Sau3AI GATC 6 cut(s) 829, 1320, 1961, 2080, 2092, 2167
Sau96I GGNCC 1 cut(s) 2313
ScaI AGTACT 1 cut(s) 2106
SchI GAGTC 1 cut(s) 1681
ScrFI CCNGG 3 cut(s) 356, 623, 1968
SduI GDGCHC 3 cut(s) 29, 1613, 1747
SfaNI GCATC 3 cut(s) 689, 963, 2179
SfcI CTRYAG 4 cut(s) 1158, 1645, 2214, 2403
Sfr274I CTCGAG 1 cut(s) 1109
SfuI TTCGAA 1 cut(s) 1759
SinI GGWCC 1 cut(s) 2313
SlaI CTCGAG 1 cut(s) 1109
SmiMI CAYNNNNRTG 6 cut(s) 1074, 1140, 1271, 1277, 1871, 2088
SmlI CTYRAG 3 cut(s) 527, 1109, 1370
SmoI CTYRAG 3 cut(s) 527, 1109, 1370
SphI GCATGC 2 cut(s) 1628, 2122
SsiI CCGC 6 cut(s) 83, 95, 141, 399, 402, 518
SspI AATATT 2 cut(s) 433, 2370
SspMI CTAG 4 cut(s) 1341, 1410, 1589, 2499
StyD4I CCNGG 3 cut(s) 354, 621, 1966
StyI CCWWGG 1 cut(s) 2096
TaaI ACNGT 4 cut(s) 1389, 1916, 2449, 2462
TaiI ACGT 2 cut(s) 1750, 1871
TaqI TCGA 3 cut(s) 804, 1110, 1759
TatI WGTACW 1 cut(s) 2104
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 6 cut(s) 379, 605, 797, 895, 1154, 1971
Tru1I TTAA 6 cut(s) 54, 507, 810, 1305, 1809, 2127
Tru9I TTAA 6 cut(s) 54, 507, 810, 1305, 1809, 2127
TscAI CASTG 1 cut(s) 2383
TseFI GTSAC 1 cut(s) 1088
TseI GCWGC 8 cut(s) 34, 37, 40, 43, 46, 426, 2198, 2351
Tsp45I GTSAC 1 cut(s) 1088
TspDTI ATGAA 7 cut(s) 1005, 1158, 1272, 1289, 1430, 1455, 1548
TspGWI ACGGA 4 cut(s) 357, 624, 873, 1441
TspRI CASTG 1 cut(s) 2383
Tth111I GACNNNGTC 1 cut(s) 118
Van91I CCANNNNNTGG 1 cut(s) 625
VpaK11BI GGWCC 1 cut(s) 2313
XapI RAATTY 5 cut(s) 467, 646, 783, 2419, 2432
XceI RCATGY 4 cut(s) 1019, 1628, 2122, 2416
XcmI CCANNNNNNNNNTGG 1 cut(s) 2037
XhoI CTCGAG 1 cut(s) 1109
XmnI GAANNNNTTC 1 cut(s) 1765
XspI CTAG 4 cut(s) 1341, 1410, 1589, 2499
ZrmI AGTACT 1 cut(s) 2106
Zsp2I ATGCAT 1 cut(s) 2120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.