MD00G1182300.v1.1

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002941
Physical Location & Seq
Reverse (-)
42918136 .. 42921099
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1182300.v1.1.491

Sequence Viewer

Length: 384 bp
ATGGGTTTTCTCCCTGGAATCGCCTGTAACAAGTTTCCTCCATGGGCTGTGCTTCTGATATGGATCGCACTTTGCATTGCGACTAATAGCAATGCTTGGTTCGGCACAGCTGTGCTTGTAACAACCATGAGAAACTTCCCTCTCAGTAGAGGCACTGTGGCTGGCATTCTCAAAGGTTATATCGGGCTTAGCACTGCTGTTTATACAGTGATATACAGTACGGTGCTTGAAGATTCTGCTCTGAATCTATTGTTGCTCCTCGCTCCTGGGCTTCCTGTTTTGTATTTAGCCTTGATGTACTTTATCCGGCCATGTACTGCAGTTCTGGAGAAGATTCTTCGGAGCATGCCCATTTTCTCTTCACCCCTGTCAGTTTTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

128

Amino Acids

13.8

Weight (kDa)

9.07

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 19 - 107 2.5e-29 Nodulin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 71
AcoI YGGCCR 1 cut(s) 308
AfaI GTAC 3 cut(s) 220, 299, 316
AgsI TTSAA 1 cut(s) 230
AjnI CCWGG 2 cut(s) 13, 265
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
AlwI GGATC 1 cut(s) 71
AoxI GGCC 1 cut(s) 308
AsuHPI GGTGA 1 cut(s) 354
BciT130I CCWGG 2 cut(s) 15, 267
BfaI CTAG 1 cut(s) 382
BfmI CTRYAG 1 cut(s) 318
BlpI GCTNAGC 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 15, 267
BmrFI CCNGG 2 cut(s) 15, 267
BpmI CTGGAG 1 cut(s) 347
Bpu1102I GCTNAGC 1 cut(s) 188
BsaBI GATNNNNATC 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 13, 41, 266
Bse3DI GCAATG 2 cut(s) 75, 97
Bse8I GATNNNNATC 1 cut(s) 62
BseBI CCWGG 2 cut(s) 15, 267
BseDI CCNNGG 3 cut(s) 13, 41, 266
BseJI GATNNNNATC 1 cut(s) 62
BseMI GCAATG 2 cut(s) 75, 97
BseMII CTCAG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 248
BshFI GGCC 1 cut(s) 310
BsiSI CCGG 1 cut(s) 307
BsmI GAATGC 1 cut(s) 165
BsnI GGCC 1 cut(s) 310
Bsp143I GATC 1 cut(s) 63
Bsp1720I GCTNAGC 1 cut(s) 188
Bsp19I CCATGG 1 cut(s) 41
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 1 cut(s) 156
BspMAI CTGCAG 1 cut(s) 322
BspPI GGATC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 75, 97
BssECI CCNNGG 3 cut(s) 13, 41, 266
BssMI GATC 1 cut(s) 63
BssT1I CCWWGG 1 cut(s) 41
Bst2UI CCWGG 2 cut(s) 15, 267
Bst4CI ACNGT 4 cut(s) 157, 208, 218, 223
Bst6I CTCTTC 1 cut(s) 364
BstC8I GCNNGC 2 cut(s) 163, 347
BstDEI CTNAG 2 cut(s) 143, 188
BstDSI CCRYGG 1 cut(s) 41
BstKTI GATC 1 cut(s) 66
BstMBI GATC 1 cut(s) 63
BstNI CCWGG 2 cut(s) 15, 267
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 2 cut(s) 13, 265
BstSFI CTRYAG 1 cut(s) 318
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 41
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 3 cut(s) 153, 192, 213
Cac8I GCNNGC 2 cut(s) 163, 347
Csp6I GTAC 3 cut(s) 219, 298, 315
CviAII CATG 4 cut(s) 42, 127, 312, 346
CviJI RGCY 7 cut(s) 47, 110, 161, 187, 271, 290, 310
CviKI_1 RGCY 7 cut(s) 47, 110, 161, 187, 271, 290, 310
CviQI GTAC 3 cut(s) 219, 298, 315
DdeI CTNAG 2 cut(s) 143, 188
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
EaeI YGGCCR 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 41
EcoRII CCWGG 2 cut(s) 13, 265
EcoT14I CCWWGG 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 41
FaeI CATG 4 cut(s) 45, 130, 315, 349
FaiI YATR 8 cut(s) 43, 61, 128, 180, 204, 214, 313, 347
FatI CATG 4 cut(s) 41, 126, 311, 345
FspBI CTAG 1 cut(s) 382
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 1 cut(s) 310
HapII CCGG 1 cut(s) 307
Hin1II CATG 4 cut(s) 45, 130, 315, 349
HinfI GANTC 4 cut(s) 18, 233, 244, 334
HpaII CCGG 1 cut(s) 307
HphI GGTGA 1 cut(s) 354
Hpy188I TCNGA 3 cut(s) 57, 243, 342
Hpy188III TCNNGA 1 cut(s) 326
HpyCH4III ACNGT 4 cut(s) 157, 208, 218, 223
HpyCH4V TGCA 2 cut(s) 75, 320
HpyF3I CTNAG 2 cut(s) 143, 188
Hsp92II CATG 4 cut(s) 45, 130, 315, 349
Kzo9I GATC 1 cut(s) 63
LmnI GCTCC 3 cut(s) 261, 268, 342
LpnPI CCDG 9 cut(s) 27, 37, 147, 252, 279, 288, 311, 320, 380
MaeI CTAG 1 cut(s) 382
MaeIII GTNAC 2 cut(s) 26, 118
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 4 cut(s) 242, 329, 343, 351
MnlI CCTC 4 cut(s) 48, 143, 150, 269
MslI CAYNNNNRTG 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 1 cut(s) 307
MspR9I CCNGG 2 cut(s) 15, 267
Mva1269I GAATGC 1 cut(s) 165
MvaI CCWGG 2 cut(s) 15, 267
NcoI CCATGG 1 cut(s) 41
NdeII GATC 1 cut(s) 63
NlaIII CATG 4 cut(s) 45, 130, 315, 349
NspI RCATGY 1 cut(s) 349
OliI CACNNNNGTG 1 cut(s) 110
PaeI GCATGC 1 cut(s) 349
PctI GAATGC 1 cut(s) 165
PfeI GAWTC 4 cut(s) 18, 233, 244, 334
Psp6I CCWGG 2 cut(s) 13, 265
PspGI CCWGG 2 cut(s) 13, 265
PstI CTGCAG 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 3 cut(s) 220, 299, 316
RsaNI GTAC 3 cut(s) 219, 298, 315
RseI CAYNNNNRTG 1 cut(s) 110
Sau3AI GATC 1 cut(s) 63
ScrFI CCNGG 2 cut(s) 15, 267
SetI ASST 2 cut(s) 112, 178
SfcI CTRYAG 1 cut(s) 318
SmiMI CAYNNNNRTG 1 cut(s) 110
SphI GCATGC 1 cut(s) 349
SspMI CTAG 1 cut(s) 382
StyD4I CCNGG 2 cut(s) 13, 265
StyI CCWWGG 1 cut(s) 41
TaaI ACNGT 4 cut(s) 157, 208, 218, 223
TatI WGTACW 2 cut(s) 297, 314
TfiI GAWTC 4 cut(s) 18, 233, 244, 334
TscAI CASTG 3 cut(s) 160, 199, 213
TspRI CASTG 3 cut(s) 160, 199, 213
XceI RCATGY 1 cut(s) 349
XspI CTAG 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.