pycom16g23280

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002941
Physical Location & Seq
Forward (+)
23966416 .. 23966832
417 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g23280.1

Sequence Viewer

Length: 417 bp
ATGCTGGGTGTGGCCAACGATATCGGCGAGAACTTTGGCCTTATCCCCGGATTCCTTTCCAAGAAGCACCCGCCGTGGCTAATTCTTTCGATCGGCGCTCTCGCCTGCTTCTTCGGCTACGGCATCATCTGGCTCACCGTCAGCACCACCATTATGCCCCTGCCTTACTGGATGCTATGGATTGCCCTTTGTGTTGCCGCTAATAGTAGTGCTTGGTTTACCACGGCTGTGATTGTAACCAACATCAAAAACTTCCCTCTACAGCGTGGCATAGTTGCTGGTATTCTGAAAGGTTACGCGGGTCTCGGCGCAGCTGTATTTACAGAAATTTATCGTGTTCTGCTTGGTAATTCCTCTTCCAGGCTTCTGCTGTTCCTTGCACTTGGAATTCCCGTTCTTCCGTCGCTTGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

139

Amino Acids

14.87

Weight (kDa)

9.1

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 1 - 133 1.3e-50 Nodulin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 299
AciI CCGC 3 cut(s) 71, 198, 299
AcoI YGGCCR 1 cut(s) 12
AcsI RAATTY 2 cut(s) 327, 387
AfiI CCNNNNNNNGG 1 cut(s) 360
AjnI CCWGG 1 cut(s) 359
AleI CACNNNNGTG 1 cut(s) 227
AloI GAACNNNNNNTCC 2 cut(s) 378, 410
AluBI AGCT 1 cut(s) 314
AluI AGCT 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 308
AoxI GGCC 2 cut(s) 12, 37
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 327, 387
AspLEI GCGC 2 cut(s) 98, 311
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 1 cut(s) 127
BalI TGGCCA 1 cut(s) 14
BarI GAAGNNNNNNTAC 2 cut(s) 340, 372
BbvI GCAGC 1 cut(s) 323
BceAI ACGGC 3 cut(s) 58, 136, 240
BciT130I CCWGG 1 cut(s) 361
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 308
BfmI CTRYAG 1 cut(s) 260
BfoI RGCGCY 1 cut(s) 99
BisI GCNGC 2 cut(s) 198, 312
BlsI GCNGC 2 cut(s) 199, 313
Bme1390I CCNGG 2 cut(s) 48, 361
BmrFI CCNGG 2 cut(s) 48, 361
BmsI GCATC 2 cut(s) 132, 162
BpuMI CCSGG 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 3 cut(s) 46, 74, 222
Bsc4I CCNNNNNNNGG 1 cut(s) 360
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 361
BseDI CCNNGG 3 cut(s) 46, 74, 222
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 323
BseYI CCCAGC 1 cut(s) 4
Bsh1236I CGCG 1 cut(s) 299
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 14, 39
BsiEI CGRYCG 1 cut(s) 93
BsiSI CCGG 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 360
BsmAI GTCTC 1 cut(s) 308
BsnI GGCC 2 cut(s) 14, 39
Bso31I GGTCTC 1 cut(s) 308
Bsp143I GATC 1 cut(s) 90
BspACI CCGC 3 cut(s) 71, 198, 299
BspANI GGCC 2 cut(s) 14, 39
BspFNI CGCG 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 308
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 3 cut(s) 46, 74, 222
BssMI GATC 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 361
BstC8I GCNNGC 1 cut(s) 106
BstDSI CCRYGG 2 cut(s) 74, 222
BstENI CCTNNNNNAGG 1 cut(s) 358
BstF5I GGATG 1 cut(s) 177
BstFNI CGCG 1 cut(s) 299
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 2 cut(s) 98, 311
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 1 cut(s) 90
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 1 cut(s) 361
BstSCI CCNGG 2 cut(s) 46, 359
BstSFI CTRYAG 1 cut(s) 260
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 1 cut(s) 323
BsuRI GGCC 2 cut(s) 14, 39
BtgI CCRYGG 2 cut(s) 74, 222
BtsCI GGATG 1 cut(s) 177
Cac8I GCNNGC 1 cut(s) 106
CfoI GCGC 2 cut(s) 98, 311
CviJI RGCY 8 cut(s) 14, 39, 79, 117, 133, 227, 314, 364
CviKI_1 RGCY 8 cut(s) 14, 39, 79, 117, 133, 227, 314, 364
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 361
EarI CTCTTC 1 cut(s) 361
Eco31I GGTCTC 1 cut(s) 308
Eco32I GATATC 1 cut(s) 22
EcoNI CCTNNNNNAGG 1 cut(s) 358
EcoRI GAATTC 1 cut(s) 387
EcoRII CCWGG 1 cut(s) 359
EcoRV GATATC 1 cut(s) 22
FaiI YATR 3 cut(s) 155, 178, 272
FauI CCCGC 2 cut(s) 78, 292
Fnu4HI GCNGC 2 cut(s) 198, 312
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 2 cut(s) 198, 312
GlaI GCGC 2 cut(s) 97, 310
GluI GCNGC 2 cut(s) 198, 312
GsaI CCCAGC 1 cut(s) 8
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 2 cut(s) 14, 39
HapII CCGG 1 cut(s) 48
HhaI GCGC 2 cut(s) 98, 311
Hin6I GCGC 2 cut(s) 96, 309
HinP1I GCGC 2 cut(s) 96, 309
HinfI GANTC 1 cut(s) 51
HpaII CCGG 1 cut(s) 48
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 1 cut(s) 219
Hpy188I TCNGA 1 cut(s) 288
Hpy8I GTNNAC 1 cut(s) 219
Hpy99I CGWCG 1 cut(s) 406
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 1 cut(s) 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HspAI GCGC 2 cut(s) 96, 309
Kzo9I GATC 1 cut(s) 90
LpnPI CCDG 8 cut(s) 61, 115, 118, 154, 173, 264, 346, 373
Lsp1109I GCAGC 1 cut(s) 323
LweI GCATC 2 cut(s) 132, 162
MaeIII GTNAC 2 cut(s) 235, 293
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 3 cut(s) 103, 348, 389
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 4 cut(s) 81, 327, 349, 387
MluNI TGGCCA 1 cut(s) 14
MnlI CCTC 2 cut(s) 267, 364
Mox20I TGGCCA 1 cut(s) 14
MscI TGGCCA 1 cut(s) 14
MslI CAYNNNNRTG 2 cut(s) 152, 227
Msp20I TGGCCA 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 48, 361
MvaI CCWGG 1 cut(s) 361
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 48
NdeII GATC 1 cut(s) 90
NmeAIII GCCGAG 1 cut(s) 285
OliI CACNNNNGTG 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 2 cut(s) 199, 313
Ple19I CGATCG 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 359
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 1 cut(s) 359
PvuI CGATCG 1 cut(s) 93
PvuII CAGCTG 1 cut(s) 314
RseI CAYNNNNRTG 2 cut(s) 152, 227
SatI GCNGC 2 cut(s) 198, 312
Sau3AI GATC 1 cut(s) 90
ScrFI CCNGG 2 cut(s) 48, 361
SetI ASST 2 cut(s) 295, 316
SfaNI GCATC 2 cut(s) 132, 162
SfcI CTRYAG 1 cut(s) 260
SmiMI CAYNNNNRTG 2 cut(s) 152, 227
Sse9I AATT 4 cut(s) 81, 327, 349, 387
SsiI CCGC 3 cut(s) 71, 198, 299
StyD4I CCNGG 2 cut(s) 46, 359
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 89
TasI AATT 4 cut(s) 81, 327, 349, 387
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 1 cut(s) 51
TseI GCWGC 1 cut(s) 311
TspGWI ACGGA 1 cut(s) 390
XagI CCTNNNNNAGG 1 cut(s) 358
XapI RAATTY 2 cut(s) 327, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.