Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0002941

Physical Location & Seq

Genomic Coordinates Forward (+)

950566 .. 953013

Gene Structure 2448 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g01580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1722 bp

ATGCCGACACTCAAAGCAGGCACCCGACCGCCGTGGGTTGGCCTAGGAGCGGCGGTGTGGGTGCAAATTGCAGCCGGCAATGCCTACACTTTCCCTCTCTACTCCCACTCCCTGAAGTCGGTTCTGGGTTTCAACCAGCAGCAGCTCACCATGCTGGGTGTGGCCAACGATATCGGCGAGAACGTTGGCCTTATCCCCGGCATCGCTTCCAACAGGCTCCCTCCGTGGCTAATTCTTTTGATCGGAGCTCTCGCCTGCTTCACCGGCTACGGCGTCATCTGGCTCACCGTCAGCCGCACCATTCAGCCCCTGCCATACTGGTTGTTATGGCTTGCCCTTTTTGTTGCCACTAATAGTAATGCTTGGTTTTCCACGGCCGTGCTTGTAACCAACATGAGAAACTTCCCTCTAACGCGTGGCACAGTTGCTGGTATTCTGAAAGGTTACGGGGGTCTCAGCGCAGCTGTATTTACAGAAGTTTATAGTGTTCTGCTTCGTAATTCCTCTTCCAGGCTTCTGCTCTTCCTTACACTTGGGATTCCTGTTCTGTGTTTTGCTATGATGTATTTTGTGTGGCCTTGTACTCCGGCTTCAAGTGAAGACCCTGCAGAGCGTGGCAACTTTCTTTTTATCCAAGTTGCAAGCGTTGTGCTTGGATTATACGTTCTCTCAACTACAATATTGGATGATACCATTTCCTTAAGTGCTCCAATTACCAACACTTTTGTCGTCATAATGGTTCTACTTCTCATGGCCCCACTTGCAATCCCTGTAAAAATGACATTGTATCCCACAAGAACCAGCAAATCGGGGATGCTTGGTCAAGCAGTTGGGTCTTCAGGTAGTTTGAACGATGAAGAAGGCACTGCAGACAAAACTGAACCACTAATGAAGCCATCTTCATCGACAGCGAGCCTGGGAAGTTTAAATGAAGGTTATGATTCATCTGACGTGGCTTTGCTTCTTGCTGAGGGCGAGGGGGCAGTGAAAAGAAAGAGACGGCCCAGAAGAGGGGAAGACTTCAAATTTAGTGAAGCTGTGGTGAAGGCTGACTTCTGGCTTCTATTCTTTGTTTACTTTTTCGGGGTTGGGTCGGGGGTAACTGTTCTCAATAATCTAGCCCAAATCGGGATTGCACAAGGATTTCATGATACAACAGTCTTGCTGTCTCTCTTCAGTTTTGGAAATTTTGTTGGTCGTCTTGGTGGAGGAGTTGTTTCCGAACATTTTGTCAGGTCGAAAACAATCCCTCGGACAGTTTGGATGACGTGCACTCAAATTATAATGATCGCCACATATCTGCTGTTTGCTTCTGCCATCAGCGCAACCTTTTATCCTGCAACGGCATTACTTGGAATCTGCTACGGTGTCCAATTTTCTGTCATGATCCCAACTGTCTCGGAACTCTTTGGCTTGAGGGATTTTGGCATATTTTACAATTTCATGTCACTAGGGAATCCTCTAGGTGCACTGCTCTTTTCAGGCCTCCTAGCCGGGTACGTATATGATAACGAGGCAGCGAAGCAGCATGGGGTTCATCTCTACGGGACAAATGTCTCCTGCGTGGGTCCGAATTGCTTTAGGCTCACCTTCCTGGTTCTCGCTGCTGTCTGTGGCGTCGGTACCATCTTAAGCATCGTTCTGTCTTTAAGGATAAAGCCTGTTTACCAAATGCTTTACTCTGGGGGCTCCTTCAGGGTACTTCCAAGTACAAACCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 Nodulin-like

Protein Analysis

574

Amino Acids

61.78

Weight (kDa)

8.9

Isoelectric Point (pI)

27.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nodulin-like	PF06813	11 - 258	1.6e-88	Nodulin-like
NFD4_C	PF23262	349 - 555	2.5e-26	NFD4, C-terminal domain
MFS_1	PF07690	356 - 551	1.8e-11	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002941)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80530
fragaria_vesca	FvH4_1g01960 FvH4_6g29100 FvH4_6g29100
malus_domestica	MD00G1182300.v1.1 MD02G1017800.v1.1 MD02G1017900.v1.1 MD17G1217900.v1.1
prunus_persica	Prupe.3G093500_v2.0.a1 Prupe.3G093500_v2.0.a1 Prupe.3G093500_v2.0.a1 Prupe.7G257000_v2.0.a1 Prupe.7G257000_v2.0.a1
pyrus_communis	pycom02g01580 pycom02g01590 pycom16g23280 pycom17g22210
rosa_chinensis	RchiOBHm_Chr2g0086971 RchiOBHm_Chr2g0134291
rosa_laevigata	RLG00000015819 RLG00000019384
rosa_multiflora	Rmu_co8300459.1_g000001 Rmu_co8420697.1_g000001 Rmu_sc0000418.1_g000001 Rmu_sc0000418.1_g000002 Rmu_sc0014586.1_g000007
rosa_roxburghii	Rroxscaffold_2G00110360 Rroxscaffold_2G00153980
rosa_rugosa	Rorug01G0471200 Rorug02G0315800 Rorug02G0315900
rosa_samantha	Rh2AG023900 Rh2AG368600 Rh2BG024400 Rh2CG024600 Rh2DG024500
rosa_wichuraiana	Rw2G001880 Rw2G030030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1283
Acc65I	GGTACC	1 cut(s)	1622
AccB1I	GGYRCC	2 cut(s)	20, 1622
AccBSI	CCGCTC	1 cut(s)	50
AccII	CGCG	1 cut(s)	415
AciI	CCGC	4 cut(s)	29, 50, 53, 295
AclI	AACGTT	1 cut(s)	183
AclWI	GGATC	1 cut(s)	1381
AcoI	YGGCCR	2 cut(s)	162, 375
AcsI	RAATTY	2 cut(s)	1025, 1186
AcuI	CTGAAG	4 cut(s)	134, 822, 1159, 1678
AcyI	GRCGYC	2 cut(s)	273, 1617
AfaI	GTAC	5 cut(s)	583, 1499, 1624, 1701, 1711
AfiI	CCNNNNNNNGG	7 cut(s)	38, 49, 118, 510, 1010, 1011, 1128
AflII	CTTAAG	2 cut(s)	700, 1630
AflIII	ACRYGT	1 cut(s)	413
AgsI	TTSAA	4 cut(s)	133, 594, 850, 1024
AjiI	CACGTC	2 cut(s)	952, 1269
AjnI	CCWGG	3 cut(s)	509, 915, 1593
AleI	CACNNNNGTG	1 cut(s)	377
AloI	GAACNNNNNNTCC	2 cut(s)	648, 680
AluBI	AGCT	4 cut(s)	145, 248, 464, 1037
AluI	AGCT	4 cut(s)	145, 248, 464, 1037
Alw21I	GWGCWC	4 cut(s)	250, 709, 1274, 1471
Alw26I	GTCTC	5 cut(s)	458, 991, 1173, 1402, 1561
Alw44I	GTGCAC	2 cut(s)	1270, 1467
AlwI	GGATC	1 cut(s)	1381
AlwNI	CAGNNNCTG	2 cut(s)	310, 428
AoxI	GGCC	8 cut(s)	40, 162, 187, 375, 575, 753, 1001, 1483
ApaLI	GTGCAC	2 cut(s)	1270, 1467
ApeKI	GCWGC	7 cut(s)	71, 139, 142, 461, 1517, 1525, 1604
ApoI	RAATTY	2 cut(s)	1025, 1186
ArsI	GACNNNNNNTTYG	2 cut(s)	1215, 1247
Asp718I	GGTACC	1 cut(s)	1622
AspA2I	CCTAGG	1 cut(s)	43
AspLEI	GCGC	2 cut(s)	461, 1325
AspS9I	GGNCC	3 cut(s)	754, 1002, 1568
AsuC2I	CCSGG	2 cut(s)	198, 1495
AsuHPI	GGTGA	5 cut(s)	139, 253, 277, 1054, 1579
AvaII	GGWCC	1 cut(s)	1568
AvrII	CCTAGG	1 cut(s)	43
BaeGI	GKGCMC	2 cut(s)	1274, 1471
BaeI	ACNNNNGTAYC	2 cut(s)	1143, 1176
BalI	TGGCCA	1 cut(s)	164
BanI	GGYRCC	2 cut(s)	20, 1622
BanII	GRGCYC	2 cut(s)	250, 1691
BarI	GAAGNNNNNNTAC	4 cut(s)	490, 522, 574, 606
BbsI	GAAGAC	3 cut(s)	606, 828, 1023
Bbv12I	GWGCWC	4 cut(s)	250, 709, 1274, 1471
BbvCI	CCTCAGC	1 cut(s)	969
BbvI	GCAGC	7 cut(s)	83, 151, 154, 473, 1529, 1537, 1591
BccI	CCATC	3 cut(s)	904, 1325, 1634
BceAI	ACGGC	6 cut(s)	16, 286, 362, 390, 1016, 1359
BciT130I	CCWGG	3 cut(s)	511, 917, 1595
BciVI	GTATCC	1 cut(s)	798
BcnI	CCSGG	2 cut(s)	198, 1495
BcoDI	GTCTC	5 cut(s)	458, 991, 1173, 1402, 1561
BfaI	CTAG	5 cut(s)	44, 1118, 1451, 1463, 1490
BfmI	CTRYAG	2 cut(s)	606, 867
BfrI	CTTAAG	2 cut(s)	700, 1630
BfuI	GTATCC	1 cut(s)	798
BisI	GCNGC	9 cut(s)	51, 72, 140, 143, 295, 462, 1518, 1526, 1605
BlnI	CCTAGG	1 cut(s)	43
BlsI	GCNGC	9 cut(s)	52, 73, 141, 144, 296, 463, 1519, 1527, 1606
Bme1390I	CCNGG	5 cut(s)	198, 511, 917, 1495, 1595
Bme18I	GGWCC	1 cut(s)	1568
BmgBI	CACGTC	2 cut(s)	952, 1269
BmgT120I	GGNCC	3 cut(s)	754, 1002, 1568
BmiI	GGNNCC	6 cut(s)	22, 218, 756, 1569, 1624, 1690
BmrFI	CCNGG	5 cut(s)	198, 511, 917, 1495, 1595
BmsI	GCATC	3 cut(s)	210, 804, 1644
BoxI	GACNNNNGTC	1 cut(s)	1553
BpiI	GAAGAC	3 cut(s)	606, 828, 1023
Bpu10I	CCTNAGC	1 cut(s)	969
BpuEI	CTTGAG	1 cut(s)	1435
BpuMI	CCSGG	2 cut(s)	198, 1495
BsaAI	YACGTR	1 cut(s)	1501
BsaHI	GRCGYC	2 cut(s)	273, 1617
BsaI	GGTCTC	1 cut(s)	458
BsaJI	CCNNGG	7 cut(s)	32, 43, 196, 224, 372, 916, 1250
BsaXI	ACNNNNNCTCC	6 cut(s)	39, 69, 92, 122, 1200, 1230
Bsc4I	CCNNNNNNNGG	7 cut(s)	38, 49, 118, 510, 1010, 1011, 1128
Bse118I	RCCGGY	2 cut(s)	74, 263
Bse1I	ACTGG	1 cut(s)	323
Bse3DI	GCAATG	1 cut(s)	85
BseBI	CCWGG	3 cut(s)	511, 917, 1595
BseDI	CCNNGG	7 cut(s)	32, 43, 196, 224, 372, 916, 1250
BseGI	GGATG	3 cut(s)	691, 819, 1269
BseLI	CCNNNNNNNGG	7 cut(s)	38, 49, 118, 510, 1010, 1011, 1128
BseMI	GCAATG	1 cut(s)	85
BseMII	CTCAG	2 cut(s)	469, 960
BseNI	ACTGG	1 cut(s)	323
BseRI	GAGGAG	1 cut(s)	1224
BseSI	GKGCMC	2 cut(s)	1274, 1471
BseX3I	CGGCCG	1 cut(s)	375
BseXI	GCAGC	7 cut(s)	83, 151, 154, 473, 1529, 1537, 1591
BseYI	CCCAGC	1 cut(s)	154
Bsh1236I	CGCG	1 cut(s)	415
Bsh1285I	CGRYCG	2 cut(s)	29, 378
BshFI	GGCC	8 cut(s)	42, 164, 189, 377, 577, 755, 1003, 1485
BshNI	GGYRCC	2 cut(s)	20, 1622
BsiEI	CGRYCG	2 cut(s)	29, 378
BsiHKAI	GWGCWC	4 cut(s)	250, 709, 1274, 1471
BsiSI	CCGG	5 cut(s)	75, 198, 264, 587, 1494
BslFI	GGGAC	1 cut(s)	1561
BslI	CCNNNNNNNGG	7 cut(s)	38, 49, 118, 510, 1010, 1011, 1128
BsmAI	GTCTC	5 cut(s)	458, 991, 1173, 1402, 1561
BsmBI	CGTCTC	1 cut(s)	991
BsmFI	GGGAC	1 cut(s)	1561
BsnI	GGCC	8 cut(s)	42, 164, 189, 377, 577, 755, 1003, 1485
Bso31I	GGTCTC	1 cut(s)	458
Bsp1286I	GDGCHC	5 cut(s)	250, 709, 1274, 1471, 1691
Bsp143I	GATC	3 cut(s)	240, 1287, 1386
BspACI	CCGC	4 cut(s)	29, 50, 53, 295
BspANI	GGCC	8 cut(s)	42, 164, 189, 377, 577, 755, 1003, 1485
BspCNI	CTCAG	2 cut(s)	468, 961
BspFNI	CGCG	1 cut(s)	415
BspHI	TCATGA	2 cut(s)	1147, 1383
BspLI	GGNNCC	6 cut(s)	22, 218, 756, 1569, 1624, 1690
BspMAI	CTGCAG	2 cut(s)	610, 871
BspPI	GGATC	1 cut(s)	1381
BspQI	GCTCTTC	1 cut(s)	527
BspT107I	GGYRCC	2 cut(s)	20, 1622
BspTI	CTTAAG	2 cut(s)	700, 1630
BspTNI	GGTCTC	1 cut(s)	458
BsrBI	CCGCTC	1 cut(s)	50
BsrDI	GCAATG	1 cut(s)	85
BsrFI	RCCGGY	2 cut(s)	74, 263
BsrI	ACTGG	1 cut(s)	323
BssAI	RCCGGY	2 cut(s)	74, 263
BssECI	CCNNGG	7 cut(s)	32, 43, 196, 224, 372, 916, 1250
BssMI	GATC	3 cut(s)	240, 1287, 1386
BssNI	GRCGYC	2 cut(s)	273, 1617
BssT1I	CCWWGG	1 cut(s)	43
Bst2UI	CCWGG	3 cut(s)	511, 917, 1595
Bst4CI	ACNGT	7 cut(s)	289, 424, 1105, 1159, 1258, 1367, 1396
Bst6I	CTCTTC	4 cut(s)	511, 527, 1003, 1178
BstACI	GRCGYC	2 cut(s)	273, 1617
BstAFI	CTTAAG	2 cut(s)	700, 1630
BstBAI	YACGTR	1 cut(s)	1501
BstC8I	GCNNGC	6 cut(s)	19, 76, 256, 333, 643, 913
BstDEI	CTNAG	2 cut(s)	455, 969
BstDSI	CCRYGG	3 cut(s)	32, 224, 372
BstENI	CCTNNNNNAGG	1 cut(s)	508
BstF5I	GGATG	3 cut(s)	691, 819, 1269
BstFNI	CGCG	1 cut(s)	415
BstHHI	GCGC	2 cut(s)	461, 1325
BstKTI	GATC	3 cut(s)	243, 1290, 1389
BstMAI	GTCTC	5 cut(s)	458, 991, 1173, 1402, 1561
BstMBI	GATC	3 cut(s)	240, 1287, 1386
BstMCI	CGRYCG	2 cut(s)	29, 378
BstMWI	GCNNNNNNNGC	5 cut(s)	80, 151, 264, 761, 1322
BstNI	CCWGG	3 cut(s)	511, 917, 1595
BstPAI	GACNNNNGTC	1 cut(s)	1553
BstSCI	CCNGG	5 cut(s)	196, 509, 915, 1493, 1593
BstSFI	CTRYAG	2 cut(s)	606, 867
BstSLI	GKGCMC	2 cut(s)	1274, 1471
BstSNI	TACGTA	1 cut(s)	1501
BstUI	CGCG	1 cut(s)	415
BstV1I	GCAGC	7 cut(s)	83, 151, 154, 473, 1529, 1537, 1591
BstV2I	GAAGAC	3 cut(s)	606, 828, 1023
BstZI	CGGCCG	1 cut(s)	375
BsuI	GTATCC	1 cut(s)	798
BsuRI	GGCC	8 cut(s)	42, 164, 189, 377, 577, 755, 1003, 1485
BtgI	CCRYGG	3 cut(s)	32, 224, 372
BtgZI	GCGATG	1 cut(s)	187
BtrI	CACGTC	2 cut(s)	952, 1269
BtsCI	GGATG	3 cut(s)	691, 819, 1269
BtsI	GCAGTG	3 cut(s)	864, 990, 1469
BtsIMutI	CAGTG	3 cut(s)	864, 990, 1469
Cac8I	GCNNGC	6 cut(s)	19, 76, 256, 333, 643, 913
CaiI	CAGNNNCTG	2 cut(s)	310, 428
CciI	TCATGA	2 cut(s)	1147, 1383
CfoI	GCGC	2 cut(s)	461, 1325
Cfr10I	RCCGGY	2 cut(s)	74, 263
Cfr13I	GGNCC	3 cut(s)	754, 1002, 1568
CseI	GACGC	2 cut(s)	262, 1606
Csp6I	GTAC	5 cut(s)	582, 1498, 1623, 1700, 1710
CviAII	CATG	7 cut(s)	151, 394, 751, 1148, 1384, 1444, 1529
CviQI	GTAC	5 cut(s)	582, 1498, 1623, 1700, 1710
DdeI	CTNAG	2 cut(s)	455, 969
DpnI	GATC	3 cut(s)	242, 1289, 1388
DpnII	GATC	3 cut(s)	240, 1287, 1386
DraI	TTTAAA	1 cut(s)	927
EaeI	YGGCCR	2 cut(s)	162, 375
EagI	CGGCCG	1 cut(s)	375
Eam1104I	CTCTTC	4 cut(s)	511, 527, 1003, 1178
EarI	CTCTTC	4 cut(s)	511, 527, 1003, 1178
Ecl136II	GAGCTC	1 cut(s)	248
EclXI	CGGCCG	1 cut(s)	375
Eco105I	TACGTA	1 cut(s)	1501
Eco130I	CCWWGG	1 cut(s)	43
Eco147I	AGGCCT	1 cut(s)	1485
Eco24I	GRGCYC	2 cut(s)	250, 1691
Eco31I	GGTCTC	1 cut(s)	458
Eco32I	GATATC	1 cut(s)	172
Eco47I	GGWCC	1 cut(s)	1568
Eco52I	CGGCCG	1 cut(s)	375
Eco53kI	GAGCTC	1 cut(s)	248
Eco57I	CTGAAG	4 cut(s)	134, 822, 1159, 1678
EcoICRI	GAGCTC	1 cut(s)	248
EcoNI	CCTNNNNNAGG	1 cut(s)	508
EcoRII	CCWGG	3 cut(s)	509, 915, 1593
EcoRV	GATATC	1 cut(s)	172
EcoT14I	CCWWGG	1 cut(s)	43
EcoT38I	GRGCYC	2 cut(s)	250, 1691
ErhI	CCWWGG	1 cut(s)	43
Esp3I	CGTCTC	1 cut(s)	991
FaeI	CATG	7 cut(s)	154, 397, 754, 1151, 1387, 1447, 1532
FalI	AAGNNNNNCTT	2 cut(s)	1037, 1069
FaqI	GGGAC	1 cut(s)	1561
FatI	CATG	7 cut(s)	150, 393, 750, 1147, 1383, 1443, 1528
Fnu4HI	GCNGC	9 cut(s)	51, 72, 140, 143, 295, 462, 1518, 1526, 1605
FokI	GGATG	3 cut(s)	698, 826, 1276
FriOI	GRGCYC	2 cut(s)	250, 1691
Fsp4HI	GCNGC	9 cut(s)	51, 72, 140, 143, 295, 462, 1518, 1526, 1605
FspBI	CTAG	5 cut(s)	44, 1118, 1451, 1463, 1490
GlaI	GCGC	2 cut(s)	460, 1324
GluI	GCNGC	9 cut(s)	51, 72, 140, 143, 295, 462, 1518, 1526, 1605
GsaI	CCCAGC	1 cut(s)	158
HaeIII	GGCC	8 cut(s)	42, 164, 189, 377, 577, 755, 1003, 1485
HapII	CCGG	5 cut(s)	75, 198, 264, 587, 1494
HgaI	GACGC	2 cut(s)	262, 1606
HhaI	GCGC	2 cut(s)	461, 1325
Hin1I	GRCGYC	2 cut(s)	273, 1617
Hin1II	CATG	7 cut(s)	154, 397, 754, 1151, 1387, 1447, 1532
Hin6I	GCGC	2 cut(s)	459, 1323
HinP1I	GCGC	2 cut(s)	459, 1323
HinfI	GANTC	4 cut(s)	538, 941, 1356, 1456
HpaII	CCGG	5 cut(s)	75, 198, 264, 587, 1494
HphI	GGTGA	5 cut(s)	139, 253, 277, 1054, 1579
Hpy166II	GTNNAC	4 cut(s)	1075, 1272, 1469, 1666
Hpy188I	TCNGA	7 cut(s)	245, 438, 949, 1222, 1254, 1402, 1572
Hpy188III	TCNNGA	3 cut(s)	1129, 1148, 1384
Hpy8I	GTNNAC	4 cut(s)	1075, 1272, 1469, 1666
Hpy99I	CGWCG	1 cut(s)	1622
HpyAV	CCTTC	5 cut(s)	854, 926, 1039, 1600, 1702
HpyCH4III	ACNGT	7 cut(s)	289, 424, 1105, 1159, 1258, 1367, 1396
HpyCH4IV	ACGT	5 cut(s)	183, 663, 951, 1268, 1500
HpyF10VI	GCNNNNNNNGC	5 cut(s)	80, 151, 264, 761, 1322
HpyF3I	CTNAG	2 cut(s)	455, 969
HpySE526I	ACGT	5 cut(s)	183, 663, 951, 1268, 1500
Hsp92I	GRCGYC	2 cut(s)	273, 1617
Hsp92II	CATG	7 cut(s)	154, 397, 754, 1151, 1387, 1447, 1532
HspAI	GCGC	2 cut(s)	459, 1323
KpnI	GGTACC	1 cut(s)	1626
KroI	GCCGGC	1 cut(s)	74
KroNI	GCCGGC	1 cut(s)	76
Kzo9I	GATC	3 cut(s)	240, 1287, 1386
LguI	GCTCTTC	1 cut(s)	527
LmnI	GCTCC	5 cut(s)	47, 222, 245, 712, 1694
Lsp1109I	GCAGC	7 cut(s)	83, 151, 154, 473, 1529, 1537, 1591
LweI	GCATC	3 cut(s)	210, 804, 1644
MaeI	CTAG	5 cut(s)	44, 1118, 1451, 1463, 1490
MaeII	ACGT	5 cut(s)	183, 663, 951, 1268, 1500
MaeIII	GTNAC	4 cut(s)	385, 443, 1099, 1446
MalI	GATC	3 cut(s)	242, 1289, 1388
MbiI	CCGCTC	1 cut(s)	50
MboI	GATC	3 cut(s)	240, 1287, 1386
MboII	GAAGA	9 cut(s)	498, 514, 611, 828, 869, 891, 1020, 1028, 1165
MhlI	GDGCHC	5 cut(s)	250, 709, 1274, 1471, 1691
MlsI	TGGCCA	1 cut(s)	164
MluI	ACGCGT	1 cut(s)	413
MluNI	TGGCCA	1 cut(s)	164
MmeI	TCCRAC	1 cut(s)	234
Mox20I	TGGCCA	1 cut(s)	164
MroNI	GCCGGC	1 cut(s)	74
MscI	TGGCCA	1 cut(s)	164
MseI	TTAA	5 cut(s)	701, 926, 1631, 1649, 1720
MslI	CAYNNNNRTG	1 cut(s)	377
Msp20I	TGGCCA	1 cut(s)	164
MspA1I	CMGCKG	1 cut(s)	464
MspCI	CTTAAG	2 cut(s)	700, 1630
MspI	CCGG	5 cut(s)	75, 198, 264, 587, 1494
MspR9I	CCNGG	5 cut(s)	198, 511, 917, 1495, 1595
MvaI	CCWGG	3 cut(s)	511, 917, 1595
MvnI	CGCG	1 cut(s)	415
MwoI	GCNNNNNNNGC	5 cut(s)	80, 151, 264, 761, 1322
NaeI	GCCGGC	1 cut(s)	76
NciI	CCSGG	2 cut(s)	198, 1495
NdeII	GATC	3 cut(s)	240, 1287, 1386
NgoMIV	GCCGGC	1 cut(s)	74
NlaIII	CATG	7 cut(s)	154, 397, 754, 1151, 1387, 1447, 1532
NlaIV	GGNNCC	6 cut(s)	22, 218, 756, 1569, 1624, 1690
NmuCI	GTSAC	1 cut(s)	1446
OliI	CACNNNNGTG	1 cut(s)	377
PagI	TCATGA	2 cut(s)	1147, 1383
PceI	AGGCCT	1 cut(s)	1485
PciSI	GCTCTTC	1 cut(s)	527
PcsI	WCGNNNNNNNCGW	2 cut(s)	174, 180
PdiI	GCCGGC	1 cut(s)	76
PfeI	GAWTC	4 cut(s)	538, 941, 1356, 1456
PkrI	GCNGC	9 cut(s)	52, 73, 141, 144, 296, 463, 1519, 1527, 1606
Ppu21I	YACGTR	1 cut(s)	1501
PshAI	GACNNNNGTC	1 cut(s)	1553
PsiI	TTATAA	1 cut(s)	1283
Psp124BI	GAGCTC	1 cut(s)	250
Psp1406I	AACGTT	1 cut(s)	183
Psp6I	CCWGG	3 cut(s)	509, 915, 1593
PspFI	CCCAGC	1 cut(s)	154
PspGI	CCWGG	3 cut(s)	509, 915, 1593
PspN4I	GGNNCC	6 cut(s)	22, 218, 756, 1569, 1624, 1690
PspPI	GGNCC	3 cut(s)	754, 1002, 1568
PstI	CTGCAG	2 cut(s)	610, 871
PstNI	CAGNNNCTG	2 cut(s)	310, 428
PvuII	CAGCTG	1 cut(s)	464
RsaI	GTAC	5 cut(s)	583, 1499, 1624, 1701, 1711
RsaNI	GTAC	5 cut(s)	582, 1498, 1623, 1700, 1710
RseI	CAYNNNNRTG	1 cut(s)	377
SacI	GAGCTC	1 cut(s)	250
SapI	GCTCTTC	1 cut(s)	527
SaqAI	TTAA	5 cut(s)	701, 926, 1631, 1649, 1720
SatI	GCNGC	9 cut(s)	51, 72, 140, 143, 295, 462, 1518, 1526, 1605
Sau3AI	GATC	3 cut(s)	240, 1287, 1386
Sau96I	GGNCC	3 cut(s)	754, 1002, 1568
ScrFI	CCNGG	5 cut(s)	198, 511, 917, 1495, 1595
SduI	GDGCHC	5 cut(s)	250, 709, 1274, 1471, 1691
SfaNI	GCATC	3 cut(s)	210, 804, 1644
SfcI	CTRYAG	2 cut(s)	606, 867
SinI	GGWCC	1 cut(s)	1568
SmiMI	CAYNNNNRTG	1 cut(s)	377
SmlI	CTYRAG	3 cut(s)	700, 1414, 1630
SmoI	CTYRAG	3 cut(s)	700, 1414, 1630
SnaBI	TACGTA	1 cut(s)	1501
SseBI	AGGCCT	1 cut(s)	1485
SsiI	CCGC	4 cut(s)	29, 50, 53, 295
SspI	AATATT	1 cut(s)	681
SspMI	CTAG	5 cut(s)	44, 1118, 1451, 1463, 1490
SstI	GAGCTC	1 cut(s)	250
StuI	AGGCCT	1 cut(s)	1485
StyD4I	CCNGG	5 cut(s)	196, 509, 915, 1493, 1593
StyI	CCWWGG	1 cut(s)	43
TaaI	ACNGT	7 cut(s)	289, 424, 1105, 1159, 1258, 1367, 1396
TaiI	ACGT	5 cut(s)	186, 666, 954, 1271, 1503
TaqI	TCGA	2 cut(s)	905, 1238
TatI	WGTACW	2 cut(s)	581, 1709
TauI	GCSGC	2 cut(s)	53, 297
TfiI	GAWTC	4 cut(s)	538, 941, 1356, 1456
Tru1I	TTAA	5 cut(s)	701, 926, 1631, 1649, 1720
Tru9I	TTAA	5 cut(s)	701, 926, 1631, 1649, 1720
TscAI	CASTG	3 cut(s)	871, 990, 1476
TseFI	GTSAC	1 cut(s)	1446
TseI	GCWGC	7 cut(s)	71, 139, 142, 461, 1517, 1525, 1604
Tsp45I	GTSAC	1 cut(s)	1446
TspDTI	ATGAA	8 cut(s)	870, 891, 905, 933, 945, 1136, 1432, 1526
TspGWI	ACGGA	1 cut(s)	213
TspRI	CASTG	3 cut(s)	871, 990, 1476
Vha464I	CTTAAG	2 cut(s)	700, 1630
VneI	GTGCAC	2 cut(s)	1270, 1467
VpaK11BI	GGWCC	1 cut(s)	1568
XagI	CCTNNNNNAGG	1 cut(s)	508
XapI	RAATTY	2 cut(s)	1025, 1186
XcmI	CCANNNNNNNNNTGG	1 cut(s)	157
XmaJI	CCTAGG	1 cut(s)	43
XspI	CTAG	5 cut(s)	44, 1118, 1451, 1463, 1490

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.