Rorug02G0315800

Rhodanese-like domain-containing protein 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001520
Physical Location & Seq
Forward (+)
38433550 .. 38434972
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0315800.1

Sequence Viewer

Length: 678 bp
ATGGGAATTTTGGTGCTTAATTTGCAGCCCGACGTCAAACAAGTAATGGATAATACTCGAGCAACAACATCAGGAACTAAAGTAGAACGGAGGGTTATTGAGAAGAACAGGAGAAACCACATGAAAATCCTCTACTCCCATCTCAACTCTCTCCTCCCAAACCAAAATTCCAAGGAACCTCTGAGTCTGCCTGAACAAATAGATGAGGCAATTAACTACATCAAAACCCTGGAGTCGAATCTGCAGAAATCCAAGCAGAAAAGGGACAACCTAAAGTTTGGTAGGAAAAGATCATATGAATTATGCAGCAGCACCTTTGAGACCAGATCAGCTGCCCCCACAAAATCGCCGCAAATTCAAATCCATGAGAATGGTTCCACCCTAGAGGTTGTTTTGAGTACTGGCCTACACAATCAGTTCATCTTCTACGATATCATTCGCATACTTGACCAAGAACAAGCAGATGTTGTACATGCTAGCTTTTCAGCTTCCGGAGACTCTATACTTCACCTAGTCCGTGCAGAGCTGGGCAAGTCGATGCTTGATTTTGGGGCAGCAAGAATAACTGAGAAGCTGAAAAGAATTGTCAATGGATCCGCAAGCGATGAAGAACTACAGCATGATGATCAAGACTACTTTTGGGACTTCCAAATCCATCCTCAGATGTGGGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

225

Amino Acids

25.83

Weight (kDa)

6.45

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 29 - 78 1.5e-08 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 2 cut(s) 350, 597
AclWI GGATC 2 cut(s) 588, 601
AcsI RAATTY 3 cut(s) 6, 166, 354
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 2 cut(s) 400, 471
AgsI TTSAA 1 cut(s) 359
AjnI CCWGG 1 cut(s) 228
AjuI GAANNNNNNNTTGG 2 cut(s) 151, 183
AluBI AGCT 5 cut(s) 332, 480, 488, 526, 574
AluI AGCT 5 cut(s) 332, 480, 488, 526, 574
Alw26I GTCTC 2 cut(s) 314, 489
AlwI GGATC 2 cut(s) 588, 601
Ama87I CYCGRG 1 cut(s) 57
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 1 cut(s) 403
ApeKI GCWGC 5 cut(s) 25, 306, 309, 332, 554
ApoI RAATTY 3 cut(s) 6, 166, 354
AsuHPI GGTGA 1 cut(s) 500
AsuNHI GCTAGC 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 57
BamHI GGATCC 1 cut(s) 593
BbvI GCAGC 5 cut(s) 37, 318, 319, 321, 566
BccI CCATC 2 cut(s) 147, 663
BciT130I CCWGG 1 cut(s) 230
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 2 cut(s) 314, 489
BfaI CTAG 3 cut(s) 383, 477, 512
BfmI CTRYAG 2 cut(s) 242, 614
BisI GCNGC 6 cut(s) 26, 307, 310, 333, 350, 555
BlsI GCNGC 6 cut(s) 27, 308, 311, 334, 351, 556
BmcAI AGTACT 1 cut(s) 400
Bme1390I CCNGG 1 cut(s) 230
BmeT110I CYCGRG 1 cut(s) 57
BmiI GGNNCC 3 cut(s) 177, 376, 595
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 528
BmtI GCTAGC 1 cut(s) 480
BpmI CTGGAG 1 cut(s) 251
BsaHI GRCGYC 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 2 cut(s) 171, 228
BsaWI WCCGGW 1 cut(s) 491
Bse1I ACTGG 1 cut(s) 406
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 171, 228
BseGI GGATG 1 cut(s) 655
BseMII CTCAG 3 cut(s) 173, 558, 674
BseNI ACTGG 1 cut(s) 406
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 5 cut(s) 37, 318, 319, 321, 566
BseYI CCCAGC 1 cut(s) 526
BsgI GTGCAG 1 cut(s) 540
BshFI GGCC 1 cut(s) 405
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 492
BslFI GGGAC 2 cut(s) 278, 656
BsmAI GTCTC 2 cut(s) 314, 489
BsmFI GGGAC 2 cut(s) 278, 656
BsnI GGCC 1 cut(s) 405
Bso31I GGTCTC 1 cut(s) 314
BsoBI CYCGRG 1 cut(s) 57
Bsp13I TCCGGA 1 cut(s) 491
Bsp1407I TGTACA 1 cut(s) 469
Bsp143I GATC 4 cut(s) 290, 326, 593, 625
BspACI CCGC 2 cut(s) 350, 597
BspANI GGCC 1 cut(s) 405
BspCNI CTCAG 3 cut(s) 174, 559, 673
BspEI TCCGGA 1 cut(s) 491
BspLI GGNNCC 3 cut(s) 177, 376, 595
BspMAI CTGCAG 1 cut(s) 246
BspOI GCTAGC 1 cut(s) 480
BspPI GGATC 2 cut(s) 588, 601
BspTNI GGTCTC 1 cut(s) 314
BsrGI TGTACA 1 cut(s) 469
BsrI ACTGG 1 cut(s) 406
BssECI CCNNGG 2 cut(s) 171, 228
BssMI GATC 4 cut(s) 290, 326, 593, 625
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 230
BstACI GRCGYC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 469
BstC8I GCNNGC 2 cut(s) 478, 601
BstDEI CTNAG 3 cut(s) 182, 567, 660
BstF5I GGATG 1 cut(s) 655
BstKTI GATC 4 cut(s) 293, 329, 596, 628
BstMAI GTCTC 2 cut(s) 314, 489
BstMBI GATC 4 cut(s) 290, 326, 593, 625
BstMWI GCNNNNNNNGC 1 cut(s) 22
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 228
BstSFI CTRYAG 2 cut(s) 242, 614
BstV1I GCAGC 5 cut(s) 37, 318, 319, 321, 566
BstX2I RGATCY 1 cut(s) 593
BstXI CCANNNNNNTGG 1 cut(s) 371
BstYI RGATCY 1 cut(s) 593
BsuRI GGCC 1 cut(s) 405
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 1 cut(s) 655
Cac8I GCNNGC 2 cut(s) 478, 601
Csp6I GTAC 2 cut(s) 399, 470
CviAII CATG 4 cut(s) 121, 365, 473, 620
CviJI RGCY 7 cut(s) 28, 332, 405, 480, 488, 526, 574
CviKI_1 RGCY 7 cut(s) 28, 332, 405, 480, 488, 526, 574
CviQI GTAC 2 cut(s) 399, 470
DdeI CTNAG 3 cut(s) 182, 567, 660
DpnI GATC 4 cut(s) 292, 328, 595, 627
DpnII GATC 4 cut(s) 290, 326, 593, 625
Eco130I CCWWGG 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 314
Eco32I GATATC 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 228
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 4 cut(s) 124, 368, 476, 623
FaiI YATR 9 cut(s) 122, 295, 297, 304, 366, 443, 474, 503, 621
FaqI GGGAC 2 cut(s) 278, 656
FatI CATG 4 cut(s) 120, 364, 472, 619
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 6 cut(s) 26, 307, 310, 333, 350, 555
FokI GGATG 1 cut(s) 642
Fsp4HI GCNGC 6 cut(s) 26, 307, 310, 333, 350, 555
FspBI CTAG 3 cut(s) 383, 477, 512
GluI GCNGC 6 cut(s) 26, 307, 310, 333, 350, 555
GsaI CCCAGC 1 cut(s) 530
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 1 cut(s) 405
HapII CCGG 1 cut(s) 492
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 4 cut(s) 124, 368, 476, 623
HinfI GANTC 4 cut(s) 184, 233, 238, 497
HpaII CCGG 1 cut(s) 492
HphI GGTGA 1 cut(s) 500
Hpy188I TCNGA 3 cut(s) 183, 663, 677
Hpy188III TCNNGA 3 cut(s) 72, 492, 629
Hpy99I CGWCG 1 cut(s) 35
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 4 cut(s) 25, 244, 306, 521
HpyF10VI GCNNNNNNNGC 1 cut(s) 22
HpyF3I CTNAG 3 cut(s) 182, 567, 660
HpySE526I ACGT 1 cut(s) 33
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 4 cut(s) 124, 368, 476, 623
Kpn2I TCCGGA 1 cut(s) 491
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 4 cut(s) 290, 326, 593, 625
LpnPI CCDG 9 cut(s) 57, 94, 204, 215, 242, 337, 387, 505, 512
Lsp1109I GCAGC 5 cut(s) 37, 318, 319, 321, 566
LweI GCATC 1 cut(s) 528
MaeI CTAG 3 cut(s) 383, 477, 512
MaeII ACGT 1 cut(s) 33
MalI GATC 4 cut(s) 292, 328, 595, 627
MboI GATC 4 cut(s) 290, 326, 593, 625
MboII GAAGA 3 cut(s) 115, 415, 620
MflI RGATCY 1 cut(s) 593
MluCI AATT 7 cut(s) 6, 19, 166, 210, 299, 354, 582
MlyI GAGTC 3 cut(s) 193, 242, 491
MnlI CCTC 7 cut(s) 84, 140, 164, 189, 199, 379, 669
MroI TCCGGA 1 cut(s) 491
MseI TTAA 2 cut(s) 18, 213
MslI CAYNNNNRTG 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 1 cut(s) 230
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 1 cut(s) 22
NdeI CATATG 1 cut(s) 295
NdeII GATC 4 cut(s) 290, 326, 593, 625
NheI GCTAGC 1 cut(s) 476
NlaIII CATG 4 cut(s) 124, 368, 476, 623
NlaIV GGNNCC 3 cut(s) 177, 376, 595
NspI RCATGY 1 cut(s) 476
PaeR7I CTCGAG 1 cut(s) 57
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 6 cut(s) 27, 308, 311, 334, 351, 556
PleI GAGTC 3 cut(s) 192, 241, 491
PpsI GAGTC 3 cut(s) 192, 241, 491
Psp6I CCWGG 1 cut(s) 228
PspFI CCCAGC 1 cut(s) 526
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 3 cut(s) 177, 376, 595
PspXI VCTCGAGB 1 cut(s) 57
PstI CTGCAG 1 cut(s) 246
PsuI RGATCY 1 cut(s) 593
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 400, 471
RsaNI GTAC 2 cut(s) 399, 470
RseI CAYNNNNRTG 1 cut(s) 369
SaqAI TTAA 2 cut(s) 18, 213
SatI GCNGC 6 cut(s) 26, 307, 310, 333, 350, 555
Sau3AI GATC 4 cut(s) 290, 326, 593, 625
ScaI AGTACT 1 cut(s) 400
SchI GAGTC 3 cut(s) 193, 242, 491
ScrFI CCNGG 1 cut(s) 230
SfaNI GCATC 1 cut(s) 528
SfcI CTRYAG 2 cut(s) 242, 614
Sfr274I CTCGAG 1 cut(s) 57
SlaI CTCGAG 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 7 cut(s) 6, 19, 166, 210, 299, 354, 582
SsiI CCGC 2 cut(s) 350, 597
SspMI CTAG 3 cut(s) 383, 477, 512
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 1 cut(s) 171
TaiI ACGT 1 cut(s) 36
TaqI TCGA 3 cut(s) 58, 236, 536
TasI AATT 7 cut(s) 6, 19, 166, 210, 299, 354, 582
TatI WGTACW 2 cut(s) 398, 469
TauI GCSGC 1 cut(s) 352
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 2 cut(s) 18, 213
Tru9I TTAA 2 cut(s) 18, 213
TseI GCWGC 5 cut(s) 25, 306, 309, 332, 554
TspDTI ATGAA 4 cut(s) 137, 312, 409, 621
TspGWI ACGGA 2 cut(s) 103, 506
XapI RAATTY 3 cut(s) 6, 166, 354
XceI RCATGY 1 cut(s) 476
XhoI CTCGAG 1 cut(s) 57
XspI CTAG 3 cut(s) 383, 477, 512
ZraI GACGTC 1 cut(s) 34
ZrmI AGTACT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.