pycom02g01570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001520
Physical Location & Seq
Forward (+)
941133 .. 941756
624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01570.1

Sequence Viewer

Length: 624 bp
ATGGCAGCTACTTCTAGAAGATCCACAAGCGCCTGCGGGCCAGTCATTCGATCCGCTCTACCCGCCGGAAGGTTTCAAAGCACTCAGTTCTCGTCTGCTTCCGCCTTCGCCTCCTCCACTTCCAGCTTCTCCTCCCGCTCCTCCACCTTCTTCAACCGCGCCGCTTCCCCCCCGCGCCTCAACGTCGCCACCGCGGCCCCCTCCGCCCAGTCCGTACGATTCTCCTTCGACCGCCCGACCTCCCCCAGCCGATCCATCTCCGTCTCGCCGCGCGGCACCGGCGCCCACCACCACCACCACACCGCCGTCAAGAGCGGCAGGAATATCAGTCAGCCGAAGCGCACCTGTATGTGTTCCCCAACCACGCACCCCGGCTCCTTCCGGTGCAGCCTCCACAAGAACAACTCCCACGTCACCGCCTCCGCGCCCTACTCGCAGAACCGACTGAACGCGCGCAGATCCGCGATGACGAACTCGCTCGTGAGAATCGGCGGCGTCGAAGGCGATCTCGTGAGGCGCGCCTTGTCCGCTCTGATCCGCCCCTCCTCCCACAGCCAGAGGCGAAGAGCCGATTTCCGTCCCCGCCCCAGCCGGCTCTGCACCATGTCTAAGTCCAACGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

22.31

Weight (kDa)

12.0

Isoelectric Point (pI)

81.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 275, 281
AccBSI CCGCTC 4 cut(s) 56, 138, 315, 530
AclWI GGATC 5 cut(s) 15, 45, 246, 453, 529
AcyI GRCGYC 2 cut(s) 282, 495
AfaI GTAC 1 cut(s) 216
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 2 cut(s) 77, 154
AjiI CACGTC 1 cut(s) 412
AluBI AGCT 2 cut(s) 8, 126
AluI AGCT 2 cut(s) 8, 126
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 5 cut(s) 15, 45, 246, 453, 529
AoxI GGCC 2 cut(s) 38, 195
ApeKI GCWGC 2 cut(s) 5, 387
AscI GGCGCGCC 1 cut(s) 517
AspS9I GGNCC 2 cut(s) 38, 196
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 1 cut(s) 406
BanI GGYRCC 2 cut(s) 275, 281
BauI CACGAG 2 cut(s) 479, 509
BbvI GCAGC 2 cut(s) 17, 399
BccI CCATC 1 cut(s) 263
BceAI ACGGC 1 cut(s) 290
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 1 cut(s) 15
BfoI RGCGCY 2 cut(s) 33, 285
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 8 cut(s) 6, 162, 195, 269, 274, 316, 388, 493
BlsI GCNGC 8 cut(s) 7, 163, 196, 270, 275, 317, 389, 494
Bme1390I CCNGG 1 cut(s) 372
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 2 cut(s) 38, 196
BmiI GGNNCC 4 cut(s) 198, 277, 283, 376
BmrFI CCNGG 1 cut(s) 372
BmrI ACTGGG 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 202
BpuMI CCSGG 1 cut(s) 372
BsaHI GRCGYC 2 cut(s) 282, 495
BsaJI CCNNGG 2 cut(s) 192, 370
BsaWI WCCGGW 1 cut(s) 381
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse118I RCCGGY 2 cut(s) 278, 591
Bse1I ACTGG 2 cut(s) 41, 208
BseDI CCNNGG 2 cut(s) 192, 370
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 2 cut(s) 41, 208
BsePI GCGCGC 2 cut(s) 452, 517
BseRI GAGGAG 4 cut(s) 103, 121, 130, 535
BseXI GCAGC 2 cut(s) 17, 399
BseYI CCCAGC 2 cut(s) 245, 587
BsgI GTGCAG 2 cut(s) 406, 583
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 2 cut(s) 40, 197
BshNI GGYRCC 2 cut(s) 275, 281
BsiEI CGRYCG 1 cut(s) 232
BsiSI CCGG 5 cut(s) 66, 279, 372, 382, 592
BsiWI CGTACG 1 cut(s) 214
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 1 cut(s) 69
BsmAI GTCTC 1 cut(s) 268
BsmBI CGTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 2 cut(s) 40, 197
Bsp143I GATC 6 cut(s) 20, 50, 251, 458, 505, 534
BspANI GGCC 2 cut(s) 40, 197
BspCNI CTCAG 1 cut(s) 97
BspLI GGNNCC 4 cut(s) 198, 277, 283, 376
BspPI GGATC 5 cut(s) 15, 45, 246, 453, 529
BspQI GCTCTTC 1 cut(s) 559
BspT107I GGYRCC 2 cut(s) 275, 281
BsrBI CCGCTC 4 cut(s) 56, 138, 315, 530
BsrFI RCCGGY 2 cut(s) 278, 591
BsrI ACTGG 2 cut(s) 41, 208
BssAI RCCGGY 2 cut(s) 278, 591
BssECI CCNNGG 2 cut(s) 192, 370
BssHII GCGCGC 2 cut(s) 452, 517
BssMI GATC 6 cut(s) 20, 50, 251, 458, 505, 534
BssNI GRCGYC 2 cut(s) 282, 495
BssSI CACGAG 2 cut(s) 479, 509
Bst2BI CACGAG 2 cut(s) 479, 509
Bst6I CTCTTC 1 cut(s) 559
BstACI GRCGYC 2 cut(s) 282, 495
BstC8I GCNNGC 5 cut(s) 34, 38, 454, 519, 593
BstDEI CTNAG 2 cut(s) 84, 609
BstDSI CCRYGG 1 cut(s) 192
BstH2I RGCGCY 2 cut(s) 33, 285
BstKTI GATC 6 cut(s) 23, 53, 254, 461, 508, 537
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 6 cut(s) 20, 50, 251, 458, 505, 534
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 8 cut(s) 62, 194, 203, 279, 433, 501, 527, 597
BstSCI CCNGG 1 cut(s) 370
BstV1I GCAGC 2 cut(s) 17, 399
BstX2I RGATCY 2 cut(s) 20, 458
BstYI RGATCY 2 cut(s) 20, 458
BsuRI GGCC 2 cut(s) 40, 197
BtgI CCRYGG 1 cut(s) 192
BtgZI GCGATG 1 cut(s) 479
BtrI CACGTC 1 cut(s) 412
Cac8I GCNNGC 5 cut(s) 34, 38, 454, 519, 593
Cfr10I RCCGGY 2 cut(s) 278, 591
Cfr13I GGNCC 2 cut(s) 38, 196
Cfr42I CCGCGG 1 cut(s) 195
CseI GACGC 1 cut(s) 484
Csp6I GTAC 1 cut(s) 215
CviAII CATG 1 cut(s) 604
CviQI GTAC 1 cut(s) 215
DdeI CTNAG 2 cut(s) 84, 609
DinI GGCGCC 1 cut(s) 283
DpnI GATC 6 cut(s) 22, 52, 253, 460, 507, 536
DpnII GATC 6 cut(s) 20, 50, 251, 458, 505, 534
Eam1104I CTCTTC 1 cut(s) 559
EarI CTCTTC 1 cut(s) 559
EciI GGCGGA 3 cut(s) 91, 193, 527
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 1 cut(s) 607
FaiI YATR 2 cut(s) 350, 605
FaqI GGGAC 1 cut(s) 564
FatI CATG 1 cut(s) 603
FauI CCCGC 5 cut(s) 29, 70, 143, 180, 590
Fnu4HI GCNGC 8 cut(s) 6, 162, 195, 269, 274, 316, 388, 493
Fsp4HI GCNGC 8 cut(s) 6, 162, 195, 269, 274, 316, 388, 493
FspBI CTAG 1 cut(s) 15
GluI GCNGC 8 cut(s) 6, 162, 195, 269, 274, 316, 388, 493
GsaI CCCAGC 2 cut(s) 249, 591
HaeII RGCGCY 2 cut(s) 33, 285
HaeIII GGCC 2 cut(s) 40, 197
HapII CCGG 5 cut(s) 66, 279, 372, 382, 592
HgaI GACGC 1 cut(s) 484
Hin1I GRCGYC 2 cut(s) 282, 495
Hin1II CATG 1 cut(s) 607
HinfI GANTC 2 cut(s) 219, 486
HpaII CCGG 5 cut(s) 66, 279, 372, 382, 592
HphI GGTGA 1 cut(s) 406
Hpy188I TCNGA 1 cut(s) 534
Hpy188III TCNNGA 4 cut(s) 15, 310, 481, 511
Hpy99I CGWCG 2 cut(s) 188, 500
HpyAV CCTTC 6 cut(s) 63, 115, 157, 235, 388, 494
HpyCH4IV ACGT 2 cut(s) 183, 411
HpyCH4V TGCA 2 cut(s) 387, 600
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 194, 203, 279, 433, 501, 527, 597
HpyF3I CTNAG 2 cut(s) 84, 609
HpySE526I ACGT 2 cut(s) 183, 411
Hsp92I GRCGYC 2 cut(s) 282, 495
Hsp92II CATG 1 cut(s) 607
KasI GGCGCC 1 cut(s) 281
KroI GCCGGC 1 cut(s) 591
KroNI GCCGGC 1 cut(s) 593
KspI CCGCGG 1 cut(s) 195
Kzo9I GATC 6 cut(s) 20, 50, 251, 458, 505, 534
LguI GCTCTTC 1 cut(s) 559
LmnI GCTCC 2 cut(s) 143, 380
Lsp1109I GCAGC 2 cut(s) 17, 399
MaeI CTAG 1 cut(s) 15
MaeII ACGT 2 cut(s) 183, 411
MaeIII GTNAC 1 cut(s) 412
MalI GATC 6 cut(s) 22, 52, 253, 460, 507, 536
MbiI CCGCTC 4 cut(s) 56, 138, 315, 530
MboI GATC 6 cut(s) 20, 50, 251, 458, 505, 534
MboII GAAGA 3 cut(s) 30, 142, 576
MflI RGATCY 2 cut(s) 20, 458
Mly113I GGCGCC 1 cut(s) 282
MroNI GCCGGC 1 cut(s) 591
MslI CAYNNNNRTG 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 5 cut(s) 66, 279, 372, 382, 592
MspR9I CCNGG 1 cut(s) 372
MwoI GCNNNNNNNGC 8 cut(s) 62, 194, 203, 279, 433, 501, 527, 597
NaeI GCCGGC 1 cut(s) 593
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 1 cut(s) 372
NdeII GATC 6 cut(s) 20, 50, 251, 458, 505, 534
NgoMIV GCCGGC 1 cut(s) 591
NlaIII CATG 1 cut(s) 607
NlaIV GGNNCC 4 cut(s) 198, 277, 283, 376
NmuCI GTSAC 1 cut(s) 412
PalAI GGCGCGCC 1 cut(s) 517
PauI GCGCGC 2 cut(s) 452, 517
PciSI GCTCTTC 1 cut(s) 559
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PdiI GCCGGC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 219, 486
Pfl23II CGTACG 1 cut(s) 214
PkrI GCNGC 8 cut(s) 7, 163, 196, 270, 275, 317, 389, 494
PluTI GGCGCC 1 cut(s) 285
PspFI CCCAGC 2 cut(s) 245, 587
PspLI CGTACG 1 cut(s) 214
PspN4I GGNNCC 4 cut(s) 198, 277, 283, 376
PspPI GGNCC 2 cut(s) 38, 196
PsuI RGATCY 2 cut(s) 20, 458
PteI GCGCGC 2 cut(s) 452, 517
RsaI GTAC 1 cut(s) 216
RsaNI GTAC 1 cut(s) 215
RseI CAYNNNNRTG 1 cut(s) 347
SacII CCGCGG 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 559
SatI GCNGC 8 cut(s) 6, 162, 195, 269, 274, 316, 388, 493
Sau3AI GATC 6 cut(s) 20, 50, 251, 458, 505, 534
Sau96I GGNCC 2 cut(s) 38, 196
ScrFI CCNGG 1 cut(s) 372
SetI ASST 8 cut(s) 10, 74, 128, 149, 186, 242, 347, 414
SfoI GGCGCC 1 cut(s) 283
Sfr303I CCGCGG 1 cut(s) 195
SgrAI CRCCGGYG 1 cut(s) 278
SgrBI CCGCGG 1 cut(s) 195
SgsI GGCGCGCC 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 347
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 370
TaiI ACGT 2 cut(s) 186, 414
TaqI TCGA 3 cut(s) 49, 228, 498
TauI GCSGC 6 cut(s) 164, 197, 271, 276, 318, 495
TfiI GAWTC 2 cut(s) 219, 486
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 2 cut(s) 5, 387
Tsp45I GTSAC 1 cut(s) 412
TspGWI ACGGA 3 cut(s) 202, 250, 566
XbaI TCTAGA 1 cut(s) 14
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.