pycom15g14540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001520
Physical Location & Seq
Forward (+)
9947656 .. 9948276
621 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14540.1

Sequence Viewer

Length: 621 bp
ATGGCAGCAACTTCAAGAAGATCCTCAAGCGGCTGCGGCCCAGTCCTCCGCTCCCCTCCACCCGCCGGAAGGTTCCAAAGCACTCAGTATTCCTCTTCCTCCGCCTTTGCCTCCTCCACTTCCGGCTTCTCCTCCCGCTCCTCCAGCTTCTTCAACCGCGCCGTCTCCCCCCCGCGCCTCAACGTCGCCACCGCCTCTCCCTCCGCCCAGTCCGTACGTTTCTCCTTCGACCGCCCAACCTCCCCCAGCCGATCCATCTCCGTCGCACCGCGCGGCACCGGCAGCCACCACCACCACCACACCGCCGTCCCCAGCGGCCGGAATATCAGTCAGCCGAAGCGAACCTGCATGTGTTCCCCTACCACGCACCCCGGGTCCTTCCGCTGCAGCCTCCACAAGAACAACTCCCACGTCACCGCCTCCGCACCGTACTCACAGAACCGACTCAACGCGCGCAGATCCGCCATGACGAACTCGCTTGTGAGAATCGGCGGCGTCGAAGGCGATCTGGTGAGGCGCGCCTTGGCCTCTCTGATCCGCCCCTCCTCCCACAGCCAGAGGCGAAGAGCTGATTTCTGTCCCCGGCCCAGCCGGCTCTCCGCCATGTCCAAGTCCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

22.07

Weight (kDa)

12.0

Isoelectric Point (pI)

88.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 275
AccBSI CCGCTC 2 cut(s) 51, 138
AccII CGCG 7 cut(s) 159, 175, 271, 273, 452, 454, 519
AclWI GGATC 4 cut(s) 15, 246, 453, 529
AcoI YGGCCR 1 cut(s) 316
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 2 cut(s) 216, 431
AfiI CCNNNNNNNGG 3 cut(s) 65, 69, 318
AgsI TTSAA 2 cut(s) 15, 154
AjiI CACGTC 1 cut(s) 412
AluBI AGCT 2 cut(s) 147, 569
AluI AGCT 2 cut(s) 147, 569
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 4 cut(s) 15, 246, 453, 529
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 4 cut(s) 37, 316, 525, 584
ApeKI GCWGC 5 cut(s) 5, 33, 282, 384, 387
AscI GGCGCGCC 1 cut(s) 517
AspLEI GCGC 7 cut(s) 161, 177, 273, 454, 456, 519, 521
AspS9I GGNCC 3 cut(s) 38, 375, 585
AsuC2I CCSGG 3 cut(s) 372, 373, 583
AsuHPI GGTGA 2 cut(s) 406, 523
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 1 cut(s) 375
BanI GGYRCC 1 cut(s) 275
BbvI GCAGC 5 cut(s) 17, 20, 294, 371, 399
BccI CCATC 1 cut(s) 263
BceAI ACGGC 2 cut(s) 146, 290
BcnI CCSGG 3 cut(s) 372, 373, 583
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 1 cut(s) 619
BfmI CTRYAG 1 cut(s) 385
BfuAI ACCTGC 1 cut(s) 353
BglI GCCNNNNNGGC 1 cut(s) 592
Bme1390I CCNGG 3 cut(s) 372, 373, 583
Bme18I GGWCC 1 cut(s) 375
BmeT110I CYCGRG 1 cut(s) 371
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 3 cut(s) 38, 375, 585
BmiI GGNNCC 3 cut(s) 74, 277, 376
BmrFI CCNGG 3 cut(s) 372, 373, 583
BmrI ACTGGG 2 cut(s) 35, 202
BmuI ACTGGG 2 cut(s) 35, 202
BpmI CTGGAG 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 10
BpuMI CCSGG 3 cut(s) 372, 373, 583
BsaHI GRCGYC 1 cut(s) 495
BsaJI CCNNGG 4 cut(s) 370, 371, 522, 581
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 69, 318
Bse118I RCCGGY 2 cut(s) 278, 591
Bse1I ACTGG 2 cut(s) 41, 208
BseDI CCNNGG 4 cut(s) 370, 371, 522, 581
BseLI CCNNNNNNNGG 3 cut(s) 65, 69, 318
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 2 cut(s) 41, 208
BsePI GCGCGC 2 cut(s) 452, 517
BseRI GAGGAG 4 cut(s) 103, 121, 130, 535
BseX3I CGGCCG 1 cut(s) 316
BseXI GCAGC 5 cut(s) 17, 20, 294, 371, 399
BseYI CCCAGC 3 cut(s) 245, 311, 587
Bsh1236I CGCG 7 cut(s) 159, 175, 271, 273, 452, 454, 519
Bsh1285I CGRYCG 2 cut(s) 232, 319
BshFI GGCC 4 cut(s) 39, 318, 527, 586
BshNI GGYRCC 1 cut(s) 275
BsiEI CGRYCG 2 cut(s) 232, 319
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 7 cut(s) 66, 123, 279, 319, 372, 583, 592
BsiWI CGTACG 1 cut(s) 214
BslFI GGGAC 2 cut(s) 293, 564
BslI CCNNNNNNNGG 3 cut(s) 65, 69, 318
BsmAI GTCTC 1 cut(s) 169
BsmBI CGTCTC 1 cut(s) 169
BsmFI GGGAC 2 cut(s) 293, 564
BsnI GGCC 4 cut(s) 39, 318, 527, 586
BsoBI CYCGRG 1 cut(s) 371
Bsp143I GATC 5 cut(s) 20, 251, 458, 505, 534
BspANI GGCC 4 cut(s) 39, 318, 527, 586
BspCNI CTCAG 1 cut(s) 97
BspFNI CGCG 7 cut(s) 159, 175, 271, 273, 452, 454, 519
BspLI GGNNCC 3 cut(s) 74, 277, 376
BspMAI CTGCAG 1 cut(s) 389
BspMI ACCTGC 1 cut(s) 353
BspPI GGATC 4 cut(s) 15, 246, 453, 529
BspQI GCTCTTC 1 cut(s) 559
BspT107I GGYRCC 1 cut(s) 275
BsrBI CCGCTC 2 cut(s) 51, 138
BsrFI RCCGGY 2 cut(s) 278, 591
BsrI ACTGG 2 cut(s) 41, 208
BssAI RCCGGY 2 cut(s) 278, 591
BssECI CCNNGG 4 cut(s) 370, 371, 522, 581
BssHII GCGCGC 2 cut(s) 452, 517
BssMI GATC 5 cut(s) 20, 251, 458, 505, 534
BssNI GRCGYC 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 522
Bst4CI ACNGT 1 cut(s) 429
Bst6I CTCTTC 2 cut(s) 100, 559
BstACI GRCGYC 1 cut(s) 495
BstC8I GCNNGC 3 cut(s) 454, 519, 593
BstDEI CTNAG 1 cut(s) 84
BstFNI CGCG 7 cut(s) 159, 175, 271, 273, 452, 454, 519
BstHHI GCGC 7 cut(s) 161, 177, 273, 454, 456, 519, 521
BstKTI GATC 5 cut(s) 23, 254, 461, 508, 537
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 5 cut(s) 20, 251, 458, 505, 534
BstMCI CGRYCG 2 cut(s) 232, 319
BstMWI GCNNNNNNNGC 6 cut(s) 36, 144, 279, 282, 501, 592
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 3 cut(s) 370, 371, 581
BstSFI CTRYAG 1 cut(s) 385
BstUI CGCG 7 cut(s) 159, 175, 271, 273, 452, 454, 519
BstV1I GCAGC 5 cut(s) 17, 20, 294, 371, 399
BstX2I RGATCY 2 cut(s) 20, 458
BstYI RGATCY 2 cut(s) 20, 458
BstZI CGGCCG 1 cut(s) 316
BsuRI GGCC 4 cut(s) 39, 318, 527, 586
BtrI CACGTC 1 cut(s) 412
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 3 cut(s) 454, 519, 593
CfoI GCGC 7 cut(s) 161, 177, 273, 454, 456, 519, 521
Cfr10I RCCGGY 2 cut(s) 278, 591
Cfr13I GGNCC 3 cut(s) 38, 375, 585
Cfr9I CCCGGG 1 cut(s) 371
CseI GACGC 1 cut(s) 484
Csp6I GTAC 2 cut(s) 215, 430
CviAII CATG 3 cut(s) 349, 466, 604
CviQI GTAC 2 cut(s) 215, 430
DdeI CTNAG 1 cut(s) 84
DpnI GATC 5 cut(s) 22, 253, 460, 507, 536
DpnII GATC 5 cut(s) 20, 251, 458, 505, 534
EaeI YGGCCR 1 cut(s) 316
EagI CGGCCG 1 cut(s) 316
Eam1104I CTCTTC 2 cut(s) 100, 559
EarI CTCTTC 2 cut(s) 100, 559
EciI GGCGGA 5 cut(s) 91, 193, 451, 527, 589
EclXI CGGCCG 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 375
Eco52I CGGCCG 1 cut(s) 316
Eco88I CYCGRG 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 522
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 3 cut(s) 352, 469, 607
FaiI YATR 3 cut(s) 350, 467, 605
FaqI GGGAC 2 cut(s) 293, 564
FatI CATG 3 cut(s) 348, 465, 603
FauI CCCGC 3 cut(s) 70, 143, 180
FspBI CTAG 1 cut(s) 619
GlaI GCGC 7 cut(s) 160, 176, 272, 453, 455, 518, 520
GsaI CCCAGC 3 cut(s) 249, 315, 591
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 4 cut(s) 39, 318, 527, 586
HapII CCGG 7 cut(s) 66, 123, 279, 319, 372, 583, 592
HgaI GACGC 1 cut(s) 484
HhaI GCGC 7 cut(s) 161, 177, 273, 454, 456, 519, 521
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 3 cut(s) 352, 469, 607
Hin6I GCGC 7 cut(s) 159, 175, 271, 452, 454, 517, 519
HinP1I GCGC 7 cut(s) 159, 175, 271, 452, 454, 517, 519
HinfI GANTC 2 cut(s) 444, 486
HpaII CCGG 7 cut(s) 66, 123, 279, 319, 372, 583, 592
HphI GGTGA 2 cut(s) 406, 523
Hpy188I TCNGA 2 cut(s) 534, 616
Hpy188III TCNNGA 1 cut(s) 15
Hpy99I CGWCG 3 cut(s) 188, 266, 500
HpyAV CCTTC 4 cut(s) 63, 235, 388, 494
HpyCH4III ACNGT 1 cut(s) 429
HpyCH4IV ACGT 3 cut(s) 183, 217, 411
HpyCH4V TGCA 2 cut(s) 348, 387
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 144, 279, 282, 501, 592
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 3 cut(s) 183, 217, 411
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 3 cut(s) 352, 469, 607
HspAI GCGC 7 cut(s) 159, 175, 271, 452, 454, 517, 519
KroI GCCGGC 1 cut(s) 591
KroNI GCCGGC 1 cut(s) 593
Kzo9I GATC 5 cut(s) 20, 251, 458, 505, 534
LguI GCTCTTC 1 cut(s) 559
LmnI GCTCC 2 cut(s) 56, 143
Lsp1109I GCAGC 5 cut(s) 17, 20, 294, 371, 399
MaeI CTAG 1 cut(s) 619
MaeII ACGT 3 cut(s) 183, 217, 411
MaeIII GTNAC 1 cut(s) 412
MalI GATC 5 cut(s) 22, 253, 460, 507, 536
MbiI CCGCTC 2 cut(s) 51, 138
MboI GATC 5 cut(s) 20, 251, 458, 505, 534
MboII GAAGA 4 cut(s) 30, 87, 142, 576
MflI RGATCY 2 cut(s) 20, 458
MlyI GAGTC 1 cut(s) 438
MroNI GCCGGC 1 cut(s) 591
MspA1I CMGCKG 2 cut(s) 315, 384
MspI CCGG 7 cut(s) 66, 123, 279, 319, 372, 583, 592
MspR9I CCNGG 3 cut(s) 372, 373, 583
MvnI CGCG 7 cut(s) 159, 175, 271, 273, 452, 454, 519
MwoI GCNNNNNNNGC 6 cut(s) 36, 144, 279, 282, 501, 592
NaeI GCCGGC 1 cut(s) 593
NciI CCSGG 3 cut(s) 372, 373, 583
NdeII GATC 5 cut(s) 20, 251, 458, 505, 534
NgoMIV GCCGGC 1 cut(s) 591
NlaIII CATG 3 cut(s) 352, 469, 607
NlaIV GGNNCC 3 cut(s) 74, 277, 376
NmuCI GTSAC 1 cut(s) 412
NspI RCATGY 1 cut(s) 352
PalAI GGCGCGCC 1 cut(s) 517
PauI GCGCGC 2 cut(s) 452, 517
PciSI GCTCTTC 1 cut(s) 559
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PdiI GCCGGC 1 cut(s) 593
PfeI GAWTC 1 cut(s) 486
Pfl23II CGTACG 1 cut(s) 214
PleI GAGTC 1 cut(s) 438
PpsI GAGTC 1 cut(s) 438
PpuMI RGGWCCY 1 cut(s) 375
Psp5II RGGWCCY 1 cut(s) 375
PspFI CCCAGC 3 cut(s) 245, 311, 587
PspLI CGTACG 1 cut(s) 214
PspN4I GGNNCC 3 cut(s) 74, 277, 376
PspPI GGNCC 3 cut(s) 38, 375, 585
PspPPI RGGWCCY 1 cut(s) 375
PstI CTGCAG 1 cut(s) 389
PsuI RGATCY 2 cut(s) 20, 458
PteI GCGCGC 2 cut(s) 452, 517
RsaI GTAC 2 cut(s) 216, 431
RsaNI GTAC 2 cut(s) 215, 430
SapI GCTCTTC 1 cut(s) 559
Sau3AI GATC 5 cut(s) 20, 251, 458, 505, 534
Sau96I GGNCC 3 cut(s) 38, 375, 585
SchI GAGTC 1 cut(s) 438
ScrFI CCNGG 3 cut(s) 372, 373, 583
SetI ASST 8 cut(s) 74, 149, 186, 220, 242, 347, 414, 571
SfcI CTRYAG 1 cut(s) 385
SgsI GGCGCGCC 1 cut(s) 517
SinI GGWCC 1 cut(s) 375
SmaI CCCGGG 1 cut(s) 373
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
SspMI CTAG 1 cut(s) 619
StyD4I CCNGG 3 cut(s) 370, 371, 581
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 1 cut(s) 429
TaiI ACGT 3 cut(s) 186, 220, 414
TaqI TCGA 2 cut(s) 228, 498
TauI GCSGC 5 cut(s) 33, 39, 276, 318, 495
TfiI GAWTC 1 cut(s) 486
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 5 cut(s) 5, 33, 282, 384, 387
Tsp45I GTSAC 1 cut(s) 412
TspGWI ACGGA 2 cut(s) 202, 250
TspMI CCCGGG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 375
XceI RCATGY 1 cut(s) 352
XmaI CCCGGG 1 cut(s) 371
XspI CTAG 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.