pycom17g22230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001520
Physical Location & Seq
Reverse (-)
20648258 .. 20649237
980 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22230.1

Sequence Viewer

Length: 855 bp
ATGAAATTAATAAGGCCATCTCGCGGCGAACAGCCCGTAAAAAATCTACAATACCAATTCACCCTCCGAAACATCACAAAATTACAAATCACCCACTCTATATATAATTTGCCACCCAACGGCGCTTTCTCGAAACACACAGAAAATTCGAACACTCAATACTCTCTCTCTAGAAAGCATAACCGTCTTATCTGTTTCTCTAACTACCATGCGACAATGGCCGTGGCTTCGTCATCGAGAACAAGATCCGACATGTACAAGCATCGCAAATCGCAGCCGTTCTTTTCCTCTTCTTCGTCGGGCTTCGCGTCGTCCACCTCCTCGAGCTTCTCCTCCGGGTCGTCGACGTTCTTCGCGCGCTCCACCTCCCCGACGCGCATCTCCATGTACGCCCACTCTCCTTCCCCGCCGCCGTCGTCGTCGCCGTCCGTACGATTCGCGAACCGCCCCGGCTCTCCCGGCCGTTCGATTTCCGTGAAGAATCAGTTACCCAACCGCAAGAACAATGACAGCAACAGCGACGCCGGCAAGAGAACGTGCCTGTGTTCCCCTACGACGCACCCTGGCTCGTTCCGGTGCGCCCTGCACAAGAACTCGCCACGTGGCGCCGCCGCTCACCAATCGCATTGTGGCGGCCAAAGTTCGTCGTATCACTCGAGTATCAGCCTGAACTACAGAAGGTCGGCGATGAAAAATTCGCTGGTGAGAATCGGAGGAGTGGAAGGCGATTTAGTGAAGAGTGCTTTGTCGGCTCTGATCAGGCCGTCGTCGCACAGCCAGCGCCGCCGGGTGGATTTTCAGCCCAAGCCGAGCCGGCTCTCCGTAATGTCCAAAGCTTCCGACGACGACGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

31.13

Weight (kDa)

10.99

Isoelectric Point (pI)

74.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 605
AccBSI CCGCTC 1 cut(s) 614
AccI GTMKAC 1 cut(s) 344
AccII CGCG 6 cut(s) 24, 308, 356, 358, 376, 440
AciI CCGC 9 cut(s) 24, 407, 410, 445, 496, 609, 612, 633, 784
AclWI GGATC 1 cut(s) 240
AcoI YGGCCR 3 cut(s) 219, 460, 634
AcsI RAATTY 2 cut(s) 145, 694
AcvI CACGTG 1 cut(s) 602
AcyI GRCGYC 2 cut(s) 522, 606
AfaI GTAC 3 cut(s) 257, 389, 432
AfiI CCNNNNNNNGG 3 cut(s) 23, 119, 790
AflIII ACRYGT 1 cut(s) 252
AjnI CCWGG 1 cut(s) 562
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 2 cut(s) 327, 836
AluI AGCT 2 cut(s) 327, 836
AlwI GGATC 1 cut(s) 240
Ama87I CYCGRG 2 cut(s) 322, 655
AoxI GGCC 5 cut(s) 14, 219, 460, 634, 761
ApeKI GCWGC 1 cut(s) 274
ApoI RAATTY 2 cut(s) 145, 694
AseI ATTAAT 1 cut(s) 8
AspLEI GCGC 7 cut(s) 125, 358, 360, 378, 581, 608, 783
AsuC2I CCSGG 4 cut(s) 337, 450, 459, 788
AsuHPI GGTGA 4 cut(s) 52, 82, 608, 715
AsuII TTCGAA 1 cut(s) 149
AvaI CYCGRG 2 cut(s) 322, 655
BaeI ACNNNNGTAYC 2 cut(s) 643, 676
BanI GGYRCC 1 cut(s) 605
BbrPI CACGTG 1 cut(s) 602
BbvI GCAGC 1 cut(s) 286
BccI CCATC 1 cut(s) 25
BceAI ACGGC 7 cut(s) 136, 206, 262, 397, 409, 447, 748
BciT130I CCWGG 1 cut(s) 564
BclI TGATCA 1 cut(s) 756
BcnI CCSGG 4 cut(s) 337, 450, 459, 788
BfaI CTAG 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 673
BfoI RGCGCY 3 cut(s) 126, 609, 784
BglI GCCNNNNNGGC 1 cut(s) 814
BisI GCNGC 7 cut(s) 25, 275, 410, 609, 612, 634, 784
BlsI GCNGC 7 cut(s) 26, 276, 411, 610, 613, 635, 785
Bme1390I CCNGG 5 cut(s) 337, 450, 459, 564, 788
BmeT110I CYCGRG 2 cut(s) 322, 655
BmiI GGNNCC 1 cut(s) 607
BmrFI CCNGG 5 cut(s) 337, 450, 459, 564, 788
BmsI GCATC 2 cut(s) 271, 387
Bpu14I TTCGAA 1 cut(s) 149
BpuMI CCSGG 4 cut(s) 337, 450, 459, 788
BsaAI YACGTR 1 cut(s) 602
BsaHI GRCGYC 2 cut(s) 522, 606
BsaJI CCNNGG 3 cut(s) 222, 448, 562
BsaWI WCCGGW 1 cut(s) 573
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 119, 790
Bse118I RCCGGY 2 cut(s) 524, 813
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 222, 448, 562
BseLI CCNNNNNNNGG 3 cut(s) 23, 119, 790
BsePI GCGCGC 1 cut(s) 356
BseRI GAGGAG 3 cut(s) 310, 322, 729
BseX3I CGGCCG 1 cut(s) 460
BseXI GCAGC 1 cut(s) 286
BsgI GTGCAG 1 cut(s) 569
Bsh1236I CGCG 6 cut(s) 24, 308, 356, 358, 376, 440
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 5 cut(s) 16, 221, 462, 636, 763
BshNI GGYRCC 1 cut(s) 605
BsiEI CGRYCG 1 cut(s) 463
BsiHKCI CYCGRG 2 cut(s) 322, 655
BsiSI CCGG 7 cut(s) 336, 450, 459, 525, 574, 787, 814
BsiWI CGTACG 1 cut(s) 430
BslI CCNNNNNNNGG 3 cut(s) 23, 119, 790
BsnI GGCC 5 cut(s) 16, 221, 462, 636, 763
BsoBI CYCGRG 2 cut(s) 322, 655
Bsp119I TTCGAA 1 cut(s) 149
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 2 cut(s) 245, 756
Bsp68I TCGCGA 1 cut(s) 440
BspACI CCGC 9 cut(s) 24, 407, 410, 445, 496, 609, 612, 633, 784
BspANI GGCC 5 cut(s) 16, 221, 462, 636, 763
BspFNI CGCG 6 cut(s) 24, 308, 356, 358, 376, 440
BspLI GGNNCC 1 cut(s) 607
BspPI GGATC 1 cut(s) 240
BspT104I TTCGAA 1 cut(s) 149
BspT107I GGYRCC 1 cut(s) 605
BsrBI CCGCTC 1 cut(s) 614
BsrFI RCCGGY 2 cut(s) 524, 813
BsrGI TGTACA 1 cut(s) 255
BssAI RCCGGY 2 cut(s) 524, 813
BssECI CCNNGG 3 cut(s) 222, 448, 562
BssHII GCGCGC 1 cut(s) 356
BssMI GATC 2 cut(s) 245, 756
BssNI GRCGYC 2 cut(s) 522, 606
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 1 cut(s) 185
Bst6I CTCTTC 2 cut(s) 295, 731
BstACI GRCGYC 2 cut(s) 522, 606
BstAUI TGTACA 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 149
BstC8I GCNNGC 4 cut(s) 358, 526, 779, 815
BstDSI CCRYGG 1 cut(s) 222
BstFNI CGCG 6 cut(s) 24, 308, 356, 358, 376, 440
BstH2I RGCGCY 3 cut(s) 126, 609, 784
BstHHI GCGC 7 cut(s) 125, 358, 360, 378, 581, 608, 783
BstKTI GATC 2 cut(s) 248, 759
BstMBI GATC 2 cut(s) 245, 756
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 8 cut(s) 218, 459, 525, 749, 769, 778, 783, 814
BstNI CCWGG 1 cut(s) 564
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 5 cut(s) 335, 448, 457, 562, 786
BstSFI CTRYAG 1 cut(s) 673
BstUI CGCG 6 cut(s) 24, 308, 356, 358, 376, 440
BstV1I GCAGC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BstZI CGGCCG 1 cut(s) 460
BsuRI GGCC 5 cut(s) 16, 221, 462, 636, 763
BtgI CCRYGG 1 cut(s) 222
BtgZI GCGATG 2 cut(s) 248, 701
BtuMI TCGCGA 1 cut(s) 440
Cac8I GCNNGC 4 cut(s) 358, 526, 779, 815
CfoI GCGC 7 cut(s) 125, 358, 360, 378, 581, 608, 783
Cfr10I RCCGGY 2 cut(s) 524, 813
CseI GACGC 4 cut(s) 297, 382, 530, 565
Csp6I GTAC 3 cut(s) 256, 388, 431
CspCI CAANNNNNGTGG 2 cut(s) 204, 239
CviAII CATG 3 cut(s) 209, 253, 385
CviQI GTAC 3 cut(s) 256, 388, 431
DinI GGCGCC 1 cut(s) 607
DpnI GATC 2 cut(s) 247, 758
DpnII GATC 2 cut(s) 245, 756
EaeI YGGCCR 3 cut(s) 219, 460, 634
EagI CGGCCG 1 cut(s) 460
Eam1104I CTCTTC 2 cut(s) 295, 731
EarI CTCTTC 2 cut(s) 295, 731
EclXI CGGCCG 1 cut(s) 460
Eco52I CGGCCG 1 cut(s) 460
Eco72I CACGTG 1 cut(s) 602
Eco88I CYCGRG 2 cut(s) 322, 655
EcoRII CCWGG 1 cut(s) 562
EgeI GGCGCC 1 cut(s) 607
EheI GGCGCC 1 cut(s) 607
FaeI CATG 3 cut(s) 212, 256, 388
FaiI YATR 7 cut(s) 101, 103, 105, 180, 210, 254, 386
FatI CATG 3 cut(s) 208, 252, 384
FauI CCCGC 1 cut(s) 414
FbaI TGATCA 1 cut(s) 756
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 7 cut(s) 25, 275, 410, 609, 612, 634, 784
Fsp4HI GCNGC 7 cut(s) 25, 275, 410, 609, 612, 634, 784
FspBI CTAG 1 cut(s) 171
GlaI GCGC 7 cut(s) 124, 357, 359, 377, 580, 607, 782
GluI GCNGC 7 cut(s) 25, 275, 410, 609, 612, 634, 784
HaeII RGCGCY 3 cut(s) 126, 609, 784
HaeIII GGCC 5 cut(s) 16, 221, 462, 636, 763
HapII CCGG 7 cut(s) 336, 450, 459, 525, 574, 787, 814
HgaI GACGC 4 cut(s) 297, 382, 530, 565
HhaI GCGC 7 cut(s) 125, 358, 360, 378, 581, 608, 783
Hin1I GRCGYC 2 cut(s) 522, 606
Hin1II CATG 3 cut(s) 212, 256, 388
Hin6I GCGC 7 cut(s) 123, 356, 358, 376, 579, 606, 781
HinP1I GCGC 7 cut(s) 123, 356, 358, 376, 579, 606, 781
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 3 cut(s) 435, 481, 708
HpaII CCGG 7 cut(s) 336, 450, 459, 525, 574, 787, 814
HphI GGTGA 4 cut(s) 52, 82, 608, 715
Hpy166II GTNNAC 2 cut(s) 315, 345
Hpy188I TCNGA 5 cut(s) 68, 250, 713, 756, 841
Hpy188III TCNNGA 4 cut(s) 130, 171, 237, 439
Hpy8I GTNNAC 2 cut(s) 315, 345
HpyAV CCTTC 3 cut(s) 411, 672, 716
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4IV ACGT 4 cut(s) 347, 536, 601, 849
HpyCH4V TGCA 1 cut(s) 586
HpyF10VI GCNNNNNNNGC 8 cut(s) 218, 459, 525, 749, 769, 778, 783, 814
HpySE526I ACGT 4 cut(s) 347, 536, 601, 849
Hsp92I GRCGYC 2 cut(s) 522, 606
Hsp92II CATG 3 cut(s) 212, 256, 388
HspAI GCGC 7 cut(s) 123, 356, 358, 376, 579, 606, 781
KasI GGCGCC 1 cut(s) 605
KroI GCCGGC 2 cut(s) 524, 813
KroNI GCCGGC 2 cut(s) 526, 815
Ksp22I TGATCA 1 cut(s) 756
Kzo9I GATC 2 cut(s) 245, 756
LmnI GCTCC 1 cut(s) 365
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 2 cut(s) 271, 387
MaeI CTAG 1 cut(s) 171
MaeII ACGT 4 cut(s) 347, 536, 601, 849
MaeIII GTNAC 1 cut(s) 486
MalI GATC 2 cut(s) 247, 758
MbiI CCGCTC 1 cut(s) 614
MboI GATC 2 cut(s) 245, 756
MboII GAAGA 5 cut(s) 282, 285, 343, 490, 748
MflI RGATCY 1 cut(s) 245
MluCI AATT 6 cut(s) 5, 56, 80, 106, 145, 694
Mly113I GGCGCC 1 cut(s) 606
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 7 cut(s) 74, 298, 328, 331, 343, 376, 707
MroNI GCCGGC 2 cut(s) 524, 813
MseI TTAA 2 cut(s) 8, 853
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 7 cut(s) 336, 450, 459, 525, 574, 787, 814
MspR9I CCNGG 5 cut(s) 337, 450, 459, 564, 788
MvaI CCWGG 1 cut(s) 564
MvnI CGCG 6 cut(s) 24, 308, 356, 358, 376, 440
MwoI GCNNNNNNNGC 8 cut(s) 218, 459, 525, 749, 769, 778, 783, 814
NaeI GCCGGC 2 cut(s) 526, 815
NarI GGCGCC 1 cut(s) 606
NciI CCSGG 4 cut(s) 337, 450, 459, 788
NdeII GATC 2 cut(s) 245, 756
NgoMIV GCCGGC 2 cut(s) 524, 813
NlaIII CATG 3 cut(s) 212, 256, 388
NlaIV GGNNCC 1 cut(s) 607
NmeAIII GCCGAG 1 cut(s) 834
NruI TCGCGA 1 cut(s) 440
NspI RCATGY 1 cut(s) 256
NspV TTCGAA 1 cut(s) 149
PaeR7I CTCGAG 2 cut(s) 322, 655
PauI GCGCGC 1 cut(s) 356
PciI ACATGT 1 cut(s) 252
PcsI WCGNNNNNNNCGW 3 cut(s) 305, 422, 653
PdiI GCCGGC 2 cut(s) 526, 815
PfeI GAWTC 3 cut(s) 435, 481, 708
Pfl23II CGTACG 1 cut(s) 430
PkrI GCNGC 7 cut(s) 26, 276, 411, 610, 613, 635, 785
PluTI GGCGCC 1 cut(s) 609
PmaCI CACGTG 1 cut(s) 602
PmlI CACGTG 1 cut(s) 602
Ppu21I YACGTR 1 cut(s) 602
PscI ACATGT 1 cut(s) 252
PshBI ATTAAT 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 562
PspCI CACGTG 1 cut(s) 602
PspGI CCWGG 1 cut(s) 562
PspLI CGTACG 1 cut(s) 430
PspN4I GGNNCC 1 cut(s) 607
PspXI VCTCGAGB 2 cut(s) 322, 655
PsrI GAACNNNNNNTAC 2 cut(s) 143, 175
PsuI RGATCY 1 cut(s) 245
PteI GCGCGC 1 cut(s) 356
RruI TCGCGA 1 cut(s) 440
RsaI GTAC 3 cut(s) 257, 389, 432
RsaNI GTAC 3 cut(s) 256, 388, 431
RseI CAYNNNNRTG 1 cut(s) 383
SalI GTCGAC 1 cut(s) 343
SaqAI TTAA 2 cut(s) 8, 853
SatI GCNGC 7 cut(s) 25, 275, 410, 609, 612, 634, 784
Sau3AI GATC 2 cut(s) 245, 756
ScrFI CCNGG 5 cut(s) 337, 450, 459, 564, 788
SetI ASST 9 cut(s) 320, 329, 350, 368, 539, 604, 683, 838, 852
SfaNI GCATC 2 cut(s) 271, 387
SfcI CTRYAG 1 cut(s) 673
SfoI GGCGCC 1 cut(s) 607
Sfr274I CTCGAG 2 cut(s) 322, 655
SfuI TTCGAA 1 cut(s) 149
SgrDI CGTCGACG 1 cut(s) 343
SlaI CTCGAG 2 cut(s) 322, 655
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 2 cut(s) 322, 655
SmoI CTYRAG 2 cut(s) 322, 655
Sse9I AATT 6 cut(s) 5, 56, 80, 106, 145, 694
SsiI CCGC 9 cut(s) 24, 407, 410, 445, 496, 609, 612, 633, 784
SspDI GGCGCC 1 cut(s) 605
SspMI CTAG 1 cut(s) 171
StyD4I CCNGG 5 cut(s) 335, 448, 457, 562, 786
TaaI ACNGT 1 cut(s) 185
TaiI ACGT 4 cut(s) 350, 539, 604, 852
TaqI TCGA 7 cut(s) 131, 149, 236, 323, 344, 467, 656
TasI AATT 6 cut(s) 5, 56, 80, 106, 145, 694
TatI WGTACW 1 cut(s) 255
TauI GCSGC 6 cut(s) 27, 412, 611, 614, 636, 786
TfiI GAWTC 3 cut(s) 435, 481, 708
Tru1I TTAA 2 cut(s) 8, 853
Tru9I TTAA 2 cut(s) 8, 853
TseI GCWGC 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 17, 704
TspGWI ACGGA 3 cut(s) 418, 463, 811
VspI ATTAAT 1 cut(s) 8
XapI RAATTY 2 cut(s) 145, 694
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 1 cut(s) 256
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
XhoI CTCGAG 2 cut(s) 322, 655
XmiI GTMKAC 1 cut(s) 344
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.