MD15G1161800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001520
Physical Location & Seq
Forward (+)
12186834 .. 12188424
1591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1161800.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGCAGCAACTTCAAGAAGATCCTCAAGCGGCTGCGGCCCAGTCCTCCGCTCCGCTCCACCACCCGCCGGAAGGTTCCAAAGCACTCGGTATTCCTCTTCCTCCGCCTTTGCTTCCTCCACTTCCAGCTTCACCTCTCGCTCCTCCACCTTCTTCAACCGCACCGTCTCCCCCCCGCGCCTCAACGTCGCCACCGCCTCTCCCTCCGCCCAGTCCGTACGTTTCTCCTTCGACCGCCCAACCTCCCCCAGCCGATCCATCTCCGTCTCACCACGCGGCACCGGCAGCCACCACCACCACCACACCGCCGTCCCCAGCGGCCGGAATATCAGTCAGCCGAAGCGAACCTGCATGTGTTCCCCTACCACACACCCCGGCTCCTTCCGCTGCAGCCTCCACAAGAACAACTCCCACGTCACCGCCTCCGCACCGTACTCGCAGAACCGACTCAACGCGCGCAGATCCGCCATGACGAACTCGCTCGTGAGAATCGGCGGCGTCGAAGGCGATCTGGTGAGGCGCGCCTTGGCCGCTCTGATCCGCCCCTCCTCCCACAGCCAGAGGCGAAGAGCCGATTTCTGTCCCCGGCCCAGCCGGCTCTCCGCCATGTCCAAGTCCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

22.25

Weight (kDa)

12.0

Isoelectric Point (pI)

87.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 278
AccBSI CCGCTC 3 cut(s) 51, 56, 533
AccII CGCG 5 cut(s) 178, 276, 455, 457, 522
AclWI GGATC 4 cut(s) 15, 249, 456, 532
AcoI YGGCCR 2 cut(s) 319, 528
AcyI GRCGYC 1 cut(s) 498
AfaI GTAC 2 cut(s) 219, 434
AfiI CCNNNNNNNGG 3 cut(s) 68, 72, 321
AgsI TTSAA 2 cut(s) 15, 157
AjiI CACGTC 1 cut(s) 415
AluBI AGCT 1 cut(s) 129
AluI AGCT 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 172, 271
AlwI GGATC 4 cut(s) 15, 249, 456, 532
AoxI GGCC 4 cut(s) 37, 319, 528, 587
ApeKI GCWGC 5 cut(s) 5, 33, 285, 387, 390
AscI GGCGCGCC 1 cut(s) 520
AspLEI GCGC 5 cut(s) 180, 457, 459, 522, 524
AspS9I GGNCC 2 cut(s) 38, 588
AsuC2I CCSGG 2 cut(s) 375, 586
AsuHPI GGTGA 4 cut(s) 124, 261, 409, 526
BanI GGYRCC 1 cut(s) 278
BauI CACGAG 1 cut(s) 482
BbvI GCAGC 5 cut(s) 17, 20, 297, 374, 402
BccI CCATC 1 cut(s) 266
BceAI ACGGC 1 cut(s) 293
BcgI CGANNNNNNTGC 2 cut(s) 417, 451
BcnI CCSGG 2 cut(s) 375, 586
BcoDI GTCTC 2 cut(s) 172, 271
BfaI CTAG 1 cut(s) 622
BfmI CTRYAG 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 356
BglI GCCNNNNNGGC 1 cut(s) 595
Bme1390I CCNGG 2 cut(s) 375, 586
BmgBI CACGTC 1 cut(s) 415
BmgT120I GGNCC 2 cut(s) 38, 588
BmiI GGNNCC 3 cut(s) 77, 280, 379
BmrFI CCNGG 2 cut(s) 375, 586
BmrI ACTGGG 2 cut(s) 35, 205
BmuI ACTGGG 2 cut(s) 35, 205
BpuEI CTTGAG 1 cut(s) 10
BpuMI CCSGG 2 cut(s) 375, 586
BsaHI GRCGYC 1 cut(s) 498
BsaJI CCNNGG 3 cut(s) 373, 525, 584
BsaXI ACNNNNNCTCC 4 cut(s) 184, 214, 362, 392
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 72, 321
Bse118I RCCGGY 2 cut(s) 281, 594
Bse1I ACTGG 2 cut(s) 41, 211
BseDI CCNNGG 3 cut(s) 373, 525, 584
BseLI CCNNNNNNNGG 3 cut(s) 68, 72, 321
BseNI ACTGG 2 cut(s) 41, 211
BsePI GCGCGC 2 cut(s) 455, 520
BseRI GAGGAG 2 cut(s) 133, 538
BseX3I CGGCCG 1 cut(s) 319
BseXI GCAGC 5 cut(s) 17, 20, 297, 374, 402
BseYI CCCAGC 3 cut(s) 248, 314, 590
Bsh1236I CGCG 5 cut(s) 178, 276, 455, 457, 522
Bsh1285I CGRYCG 2 cut(s) 235, 322
BshFI GGCC 4 cut(s) 39, 321, 530, 589
BshNI GGYRCC 1 cut(s) 278
BsiEI CGRYCG 2 cut(s) 235, 322
BsiSI CCGG 6 cut(s) 69, 282, 322, 375, 586, 595
BsiWI CGTACG 1 cut(s) 217
BslFI GGGAC 2 cut(s) 296, 567
BslI CCNNNNNNNGG 3 cut(s) 68, 72, 321
BsmAI GTCTC 2 cut(s) 172, 271
BsmBI CGTCTC 2 cut(s) 172, 271
BsmFI GGGAC 2 cut(s) 296, 567
BsnI GGCC 4 cut(s) 39, 321, 530, 589
Bsp143I GATC 5 cut(s) 20, 254, 461, 508, 537
BspANI GGCC 4 cut(s) 39, 321, 530, 589
BspFNI CGCG 5 cut(s) 178, 276, 455, 457, 522
BspLI GGNNCC 3 cut(s) 77, 280, 379
BspMAI CTGCAG 1 cut(s) 392
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 4 cut(s) 15, 249, 456, 532
BspQI GCTCTTC 1 cut(s) 562
BspT107I GGYRCC 1 cut(s) 278
BsrBI CCGCTC 3 cut(s) 51, 56, 533
BsrFI RCCGGY 2 cut(s) 281, 594
BsrI ACTGG 2 cut(s) 41, 211
BssAI RCCGGY 2 cut(s) 281, 594
BssECI CCNNGG 3 cut(s) 373, 525, 584
BssHII GCGCGC 2 cut(s) 455, 520
BssMI GATC 5 cut(s) 20, 254, 461, 508, 537
BssNI GRCGYC 1 cut(s) 498
BssSI CACGAG 1 cut(s) 482
BssT1I CCWWGG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 482
Bst4CI ACNGT 2 cut(s) 166, 432
Bst6I CTCTTC 2 cut(s) 103, 562
BstACI GRCGYC 1 cut(s) 498
BstC8I GCNNGC 3 cut(s) 457, 522, 596
BstFNI CGCG 5 cut(s) 178, 276, 455, 457, 522
BstHHI GCGC 5 cut(s) 180, 457, 459, 522, 524
BstKTI GATC 5 cut(s) 23, 257, 464, 511, 540
BstMAI GTCTC 2 cut(s) 172, 271
BstMBI GATC 5 cut(s) 20, 254, 461, 508, 537
BstMCI CGRYCG 2 cut(s) 235, 322
BstMWI GCNNNNNNNGC 7 cut(s) 36, 282, 285, 384, 504, 530, 595
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 2 cut(s) 373, 584
BstSFI CTRYAG 1 cut(s) 388
BstUI CGCG 5 cut(s) 178, 276, 455, 457, 522
BstV1I GCAGC 5 cut(s) 17, 20, 297, 374, 402
BstX2I RGATCY 2 cut(s) 20, 461
BstYI RGATCY 2 cut(s) 20, 461
BstZI CGGCCG 1 cut(s) 319
BsuRI GGCC 4 cut(s) 39, 321, 530, 589
BtrI CACGTC 1 cut(s) 415
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 3 cut(s) 457, 522, 596
CfoI GCGC 5 cut(s) 180, 457, 459, 522, 524
Cfr10I RCCGGY 2 cut(s) 281, 594
Cfr13I GGNCC 2 cut(s) 38, 588
CseI GACGC 1 cut(s) 487
Csp6I GTAC 2 cut(s) 218, 433
CviAII CATG 3 cut(s) 352, 469, 607
CviQI GTAC 2 cut(s) 218, 433
DpnI GATC 5 cut(s) 22, 256, 463, 510, 539
DpnII GATC 5 cut(s) 20, 254, 461, 508, 537
EaeI YGGCCR 2 cut(s) 319, 528
EagI CGGCCG 1 cut(s) 319
Eam1104I CTCTTC 2 cut(s) 103, 562
EarI CTCTTC 2 cut(s) 103, 562
EciI GGCGGA 5 cut(s) 94, 196, 454, 530, 592
EclXI CGGCCG 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 525
Eco52I CGGCCG 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 2 cut(s) 172, 271
FaeI CATG 3 cut(s) 355, 472, 610
FaiI YATR 3 cut(s) 353, 470, 608
FaqI GGGAC 2 cut(s) 296, 567
FatI CATG 3 cut(s) 351, 468, 606
FauI CCCGC 2 cut(s) 73, 183
FspBI CTAG 1 cut(s) 622
GlaI GCGC 5 cut(s) 179, 456, 458, 521, 523
GsaI CCCAGC 3 cut(s) 252, 318, 594
HaeIII GGCC 4 cut(s) 39, 321, 530, 589
HapII CCGG 6 cut(s) 69, 282, 322, 375, 586, 595
HgaI GACGC 1 cut(s) 487
HhaI GCGC 5 cut(s) 180, 457, 459, 522, 524
Hin1I GRCGYC 1 cut(s) 498
Hin1II CATG 3 cut(s) 355, 472, 610
Hin6I GCGC 5 cut(s) 178, 455, 457, 520, 522
HinP1I GCGC 5 cut(s) 178, 455, 457, 520, 522
HinfI GANTC 2 cut(s) 447, 489
HpaII CCGG 6 cut(s) 69, 282, 322, 375, 586, 595
HphI GGTGA 4 cut(s) 124, 261, 409, 526
Hpy188I TCNGA 2 cut(s) 537, 619
Hpy188III TCNNGA 2 cut(s) 15, 484
Hpy99I CGWCG 2 cut(s) 191, 503
HpyAV CCTTC 5 cut(s) 66, 160, 238, 391, 497
HpyCH4III ACNGT 2 cut(s) 166, 432
HpyCH4IV ACGT 3 cut(s) 186, 220, 414
HpyCH4V TGCA 2 cut(s) 351, 390
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 282, 285, 384, 504, 530, 595
HpySE526I ACGT 3 cut(s) 186, 220, 414
Hsp92I GRCGYC 1 cut(s) 498
Hsp92II CATG 3 cut(s) 355, 472, 610
HspAI GCGC 5 cut(s) 178, 455, 457, 520, 522
KroI GCCGGC 1 cut(s) 594
KroNI GCCGGC 1 cut(s) 596
Kzo9I GATC 5 cut(s) 20, 254, 461, 508, 537
LguI GCTCTTC 1 cut(s) 562
LmnI GCTCC 4 cut(s) 56, 61, 146, 383
Lsp1109I GCAGC 5 cut(s) 17, 20, 297, 374, 402
MaeI CTAG 1 cut(s) 622
MaeII ACGT 3 cut(s) 186, 220, 414
MaeIII GTNAC 1 cut(s) 415
MalI GATC 5 cut(s) 22, 256, 463, 510, 539
MbiI CCGCTC 3 cut(s) 51, 56, 533
MboI GATC 5 cut(s) 20, 254, 461, 508, 537
MboII GAAGA 4 cut(s) 30, 90, 145, 579
MflI RGATCY 2 cut(s) 20, 461
MlyI GAGTC 1 cut(s) 441
MroNI GCCGGC 1 cut(s) 594
MspA1I CMGCKG 2 cut(s) 318, 387
MspI CCGG 6 cut(s) 69, 282, 322, 375, 586, 595
MspR9I CCNGG 2 cut(s) 375, 586
MvnI CGCG 5 cut(s) 178, 276, 455, 457, 522
MwoI GCNNNNNNNGC 7 cut(s) 36, 282, 285, 384, 504, 530, 595
NaeI GCCGGC 1 cut(s) 596
NciI CCSGG 2 cut(s) 375, 586
NdeII GATC 5 cut(s) 20, 254, 461, 508, 537
NgoMIV GCCGGC 1 cut(s) 594
NlaIII CATG 3 cut(s) 355, 472, 610
NlaIV GGNNCC 3 cut(s) 77, 280, 379
NmuCI GTSAC 1 cut(s) 415
NspI RCATGY 1 cut(s) 355
PalAI GGCGCGCC 1 cut(s) 520
PauI GCGCGC 2 cut(s) 455, 520
PciSI GCTCTTC 1 cut(s) 562
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PdiI GCCGGC 1 cut(s) 596
PfeI GAWTC 1 cut(s) 489
Pfl23II CGTACG 1 cut(s) 217
PleI GAGTC 1 cut(s) 441
PpsI GAGTC 1 cut(s) 441
PspFI CCCAGC 3 cut(s) 248, 314, 590
PspLI CGTACG 1 cut(s) 217
PspN4I GGNNCC 3 cut(s) 77, 280, 379
PspPI GGNCC 2 cut(s) 38, 588
PstI CTGCAG 1 cut(s) 392
PsuI RGATCY 2 cut(s) 20, 461
PteI GCGCGC 2 cut(s) 455, 520
RsaI GTAC 2 cut(s) 219, 434
RsaNI GTAC 2 cut(s) 218, 433
SapI GCTCTTC 1 cut(s) 562
Sau3AI GATC 5 cut(s) 20, 254, 461, 508, 537
Sau96I GGNCC 2 cut(s) 38, 588
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 2 cut(s) 375, 586
SetI ASST 9 cut(s) 77, 131, 137, 152, 189, 223, 245, 350, 417
SfcI CTRYAG 1 cut(s) 388
SgsI GGCGCGCC 1 cut(s) 520
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
SspMI CTAG 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 373, 584
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 2 cut(s) 166, 432
TaiI ACGT 3 cut(s) 189, 223, 417
TaqI TCGA 2 cut(s) 231, 501
TauI GCSGC 6 cut(s) 33, 39, 279, 321, 498, 533
TfiI GAWTC 1 cut(s) 489
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 5 cut(s) 5, 33, 285, 387, 390
Tsp45I GTSAC 1 cut(s) 415
TspGWI ACGGA 2 cut(s) 205, 253
XceI RCATGY 1 cut(s) 355
XspI CTAG 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.