MD08G1152900.v1.1

Serine-rich protein-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001520
Physical Location & Seq
Reverse (-)
16531067 .. 16532527
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1152900.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGCGACCGCATCTTCCTCATCGAGGGCGAGAACCCGGGCCTACAAAAGCGGCCCTGTGCTCCGGTCGCTCTCGCCTTCCGAAAGGTTCTACACCGCTTCCAATGGCGGGATCTCGTCATCGGCGTCAGGTTTCGCTTCTTCGACGAGCTCAAGCTTCTCGTCGCCGGCGTCAGCTTTCTTCCACCAGGACCACCACTACTACAATCAGCAGATCAACCACCACCACCACCATCACAGATCCGCTTCTCCGACGCGTGTCAACCTTTACACCTCCTCCTCTCCGTCTCGGTACGTCCGGTTCTCCATCGACCACCGTTCGATCTCTCCCAACCACCATTCGAATCGATCCATCGCCGTCTCCAAGAAAACTGGACCGATTTCGATGCCGAAGAAGACCTGTATGTGCTCGCCGACGTCGCATCCGGGATCATTTCGCTGCAGCCTCCACAAAAACCAGGGAGGGAGTCAAAACACCGGATCTTTCCCGTCGAATAGGTTGAATATGCGCAGGTCTGCTATGACGAACTCGCTGGTACGAATCAGTGGAGTCGAAGGCGAGTGGGTGAAGCGAGCCTTGACCGCCCTGATTCGCCCTTGTGCGCACCAGCAGAGGCGGAGAGCCGGGTTCCAGCCACGGCCCAGCAGACTCTCTGCCATGTCCACAGCCGACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

24.78

Weight (kDa)

11.49

Isoelectric Point (pI)

70.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 419
Acc16I TGCGCA 2 cut(s) 509, 603
Acc36I ACCTGC 1 cut(s) 501
AccII CGCG 1 cut(s) 256
AciI CCGC 7 cut(s) 9, 51, 96, 108, 243, 582, 616
AclWI GGATC 5 cut(s) 119, 234, 342, 436, 487
AcyI GRCGYC 3 cut(s) 125, 170, 416
AfaI GTAC 2 cut(s) 293, 537
AfiI CCNNNNNNNGG 2 cut(s) 24, 108
AflIII ACRYGT 1 cut(s) 254
AgsI TTSAA 1 cut(s) 502
AjnI CCWGG 2 cut(s) 186, 456
AluBI AGCT 3 cut(s) 150, 156, 176
AluI AGCT 3 cut(s) 150, 156, 176
Alw21I GWGCWC 3 cut(s) 63, 152, 410
Alw26I GTCTC 2 cut(s) 291, 364
AlwI GGATC 5 cut(s) 119, 234, 342, 436, 487
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 3 cut(s) 39, 52, 638
ApeKI GCWGC 2 cut(s) 438, 441
AspLEI GCGC 2 cut(s) 510, 604
AspS9I GGNCC 5 cut(s) 39, 53, 190, 374, 639
AsuC2I CCSGG 4 cut(s) 37, 38, 426, 625
AsuHPI GGTGA 1 cut(s) 577
AsuII TTCGAA 1 cut(s) 341
AvaI CYCGRG 1 cut(s) 36
AvaII GGWCC 2 cut(s) 190, 374
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 401
Bbv12I GWGCWC 3 cut(s) 63, 152, 410
BbvI GCAGC 2 cut(s) 425, 453
BccI CCATC 3 cut(s) 240, 314, 359
BceAI ACGGC 2 cut(s) 341, 653
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BciT130I CCWGG 2 cut(s) 188, 458
BcnI CCSGG 4 cut(s) 37, 38, 426, 625
BcoDI GTCTC 2 cut(s) 291, 364
BfaI CTAG 1 cut(s) 676
BfmI CTRYAG 1 cut(s) 439
BfuAI ACCTGC 1 cut(s) 501
BisI GCNGC 3 cut(s) 52, 439, 442
BlsI GCNGC 3 cut(s) 53, 440, 443
Bme1390I CCNGG 6 cut(s) 37, 38, 188, 426, 458, 625
Bme18I GGWCC 2 cut(s) 190, 374
BmeT110I CYCGRG 1 cut(s) 36
BmgT120I GGNCC 5 cut(s) 39, 53, 190, 374, 639
BmiI GGNNCC 1 cut(s) 629
BmrFI CCNGG 6 cut(s) 37, 38, 188, 426, 458, 625
BmsI GCATC 3 cut(s) 20, 375, 430
BoxI GACNNNNGTC 1 cut(s) 257
BpiI GAAGAC 1 cut(s) 401
Bpu14I TTCGAA 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 136
BpuMI CCSGG 4 cut(s) 37, 38, 426, 625
Bsa29I ATCGAT 1 cut(s) 346
BsaHI GRCGYC 3 cut(s) 125, 170, 416
BsaJI CCNNGG 3 cut(s) 36, 457, 635
BsaWI WCCGGW 3 cut(s) 63, 297, 476
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 108
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 1 cut(s) 376
BseBI CCWGG 2 cut(s) 188, 458
BseCI ATCGAT 1 cut(s) 346
BseDI CCNNGG 3 cut(s) 36, 457, 635
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 2 cut(s) 24, 108
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 2 cut(s) 265, 268
BseXI GCAGC 2 cut(s) 425, 453
BseYI CCCAGC 1 cut(s) 641
Bsh1236I CGCG 1 cut(s) 256
Bsh1285I CGRYCG 2 cut(s) 9, 68
BshFI GGCC 3 cut(s) 41, 54, 640
BshVI ATCGAT 1 cut(s) 346
BsiEI CGRYCG 2 cut(s) 9, 68
BsiHKAI GWGCWC 3 cut(s) 63, 152, 410
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 7 cut(s) 37, 64, 167, 298, 425, 477, 624
BslI CCNNNNNNNGG 2 cut(s) 24, 108
BsmAI GTCTC 2 cut(s) 291, 364
BsmBI CGTCTC 2 cut(s) 291, 364
BsnI GGCC 3 cut(s) 41, 54, 640
BsoBI CYCGRG 1 cut(s) 36
Bsp119I TTCGAA 1 cut(s) 341
Bsp1286I GDGCHC 3 cut(s) 63, 152, 410
Bsp143I GATC 7 cut(s) 111, 213, 239, 321, 347, 428, 479
BspACI CCGC 7 cut(s) 9, 51, 96, 108, 243, 582, 616
BspANI GGCC 3 cut(s) 41, 54, 640
BspDI ATCGAT 1 cut(s) 346
BspFNI CGCG 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 629
BspMAI CTGCAG 1 cut(s) 443
BspMI ACCTGC 1 cut(s) 501
BspPI GGATC 5 cut(s) 119, 234, 342, 436, 487
BspT104I TTCGAA 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 166
BsrI ACTGG 1 cut(s) 376
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 3 cut(s) 36, 457, 635
BssMI GATC 7 cut(s) 111, 213, 239, 321, 347, 428, 479
BssNI GRCGYC 3 cut(s) 125, 170, 416
Bst2UI CCWGG 2 cut(s) 188, 458
Bst4CI ACNGT 1 cut(s) 317
BstACI GRCGYC 3 cut(s) 125, 170, 416
BstBI TTCGAA 1 cut(s) 341
BstC8I GCNNGC 3 cut(s) 168, 410, 573
BstDSI CCRYGG 1 cut(s) 635
BstENI CCTNNNNNAGG 1 cut(s) 22
BstF5I GGATG 1 cut(s) 421
BstFNI CGCG 1 cut(s) 256
BstHHI GCGC 2 cut(s) 510, 604
BstKTI GATC 7 cut(s) 114, 216, 242, 324, 350, 431, 482
BstMAI GTCTC 2 cut(s) 291, 364
BstMBI GATC 7 cut(s) 111, 213, 239, 321, 347, 428, 479
BstMCI CGRYCG 2 cut(s) 9, 68
BstMWI GCNNNNNNNGC 3 cut(s) 67, 418, 581
BstNI CCWGG 2 cut(s) 188, 458
BstPAI GACNNNNGTC 1 cut(s) 257
BstSCI CCNGG 6 cut(s) 35, 36, 186, 424, 456, 623
BstSFI CTRYAG 1 cut(s) 439
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 2 cut(s) 425, 453
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 3 cut(s) 111, 239, 479
BstYI RGATCY 3 cut(s) 111, 239, 479
Bsu15I ATCGAT 1 cut(s) 346
BsuRI GGCC 3 cut(s) 41, 54, 640
BsuTUI ATCGAT 1 cut(s) 346
BtgI CCRYGG 1 cut(s) 635
BtgZI GCGATG 1 cut(s) 337
BtsCI GGATG 1 cut(s) 421
BtsIMutI CAGTG 1 cut(s) 550
BveI ACCTGC 1 cut(s) 501
Cac8I GCNNGC 3 cut(s) 168, 410, 573
CfoI GCGC 2 cut(s) 510, 604
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 5 cut(s) 39, 53, 190, 374, 639
Cfr9I CCCGGG 1 cut(s) 36
ClaI ATCGAT 1 cut(s) 346
CseI GACGC 3 cut(s) 114, 159, 262
Csp6I GTAC 2 cut(s) 292, 536
CviAII CATG 1 cut(s) 658
CviQI GTAC 2 cut(s) 292, 536
DpnI GATC 7 cut(s) 113, 215, 241, 323, 349, 430, 481
DpnII GATC 7 cut(s) 111, 213, 239, 321, 347, 428, 479
EciI GGCGGA 1 cut(s) 631
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 190, 374
Eco53kI GAGCTC 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 36
EcoICRI GAGCTC 1 cut(s) 150
EcoNI CCTNNNNNAGG 1 cut(s) 22
EcoRII CCWGG 2 cut(s) 186, 456
EcoT38I GRGCYC 1 cut(s) 152
Esp3I CGTCTC 2 cut(s) 291, 364
FaeI CATG 1 cut(s) 661
FaiI YATR 4 cut(s) 404, 506, 521, 659
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FatI CATG 1 cut(s) 657
FauI CCCGC 1 cut(s) 101
Fnu4HI GCNGC 3 cut(s) 52, 439, 442
FokI GGATG 1 cut(s) 408
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 3 cut(s) 52, 439, 442
FspAI RTGCGCAY 1 cut(s) 603
FspBI CTAG 1 cut(s) 676
FspI TGCGCA 2 cut(s) 509, 603
GlaI GCGC 2 cut(s) 509, 603
GluI GCNGC 3 cut(s) 52, 439, 442
GsaI CCCAGC 1 cut(s) 645
HaeIII GGCC 3 cut(s) 41, 54, 640
HapII CCGG 7 cut(s) 37, 64, 167, 298, 425, 477, 624
HgaI GACGC 3 cut(s) 114, 159, 262
HhaI GCGC 2 cut(s) 510, 604
Hin1I GRCGYC 3 cut(s) 125, 170, 416
Hin1II CATG 1 cut(s) 661
Hin6I GCGC 2 cut(s) 508, 602
HinP1I GCGC 2 cut(s) 508, 602
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 6 cut(s) 343, 466, 540, 549, 589, 648
HpaII CCGG 7 cut(s) 37, 64, 167, 298, 425, 477, 624
HphI GGTGA 1 cut(s) 577
Hpy166II GTNNAC 2 cut(s) 262, 663
Hpy188I TCNGA 2 cut(s) 82, 252
Hpy8I GTNNAC 2 cut(s) 262, 663
Hpy99I CGWCG 6 cut(s) 148, 166, 256, 418, 421, 493
HpyAV CCTTC 2 cut(s) 87, 548
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 2 cut(s) 294, 416
HpyCH4V TGCA 1 cut(s) 441
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 418, 581
HpySE526I ACGT 2 cut(s) 294, 416
Hsp92I GRCGYC 3 cut(s) 125, 170, 416
Hsp92II CATG 1 cut(s) 661
HspAI GCGC 2 cut(s) 508, 602
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
Kzo9I GATC 7 cut(s) 111, 213, 239, 321, 347, 428, 479
LmnI GCTCC 1 cut(s) 66
Lsp1109I GCAGC 2 cut(s) 425, 453
LweI GCATC 3 cut(s) 20, 375, 430
MaeI CTAG 1 cut(s) 676
MaeII ACGT 2 cut(s) 294, 416
MalI GATC 7 cut(s) 113, 215, 241, 323, 349, 430, 481
MboI GATC 7 cut(s) 111, 213, 239, 321, 347, 428, 479
MboII GAAGA 5 cut(s) 6, 132, 172, 403, 406
MflI RGATCY 3 cut(s) 111, 239, 479
MhlI GDGCHC 3 cut(s) 63, 152, 410
MluI ACGCGT 1 cut(s) 254
MlyI GAGTC 3 cut(s) 475, 558, 642
MmeI TCCRAC 1 cut(s) 275
MnlI CCTC 8 cut(s) 18, 28, 283, 286, 289, 455, 455, 606
MreI CGCCGGCG 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 166
MspI CCGG 7 cut(s) 37, 64, 167, 298, 425, 477, 624
MspR9I CCNGG 6 cut(s) 37, 38, 188, 426, 458, 625
MvaI CCWGG 2 cut(s) 188, 458
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 3 cut(s) 67, 418, 581
NaeI GCCGGC 1 cut(s) 168
NciI CCSGG 4 cut(s) 37, 38, 426, 625
NdeII GATC 7 cut(s) 111, 213, 239, 321, 347, 428, 479
NgoMIV GCCGGC 1 cut(s) 166
NlaIII CATG 1 cut(s) 661
NlaIV GGNNCC 1 cut(s) 629
NsbI TGCGCA 2 cut(s) 509, 603
NspV TTCGAA 1 cut(s) 341
PcsI WCGNNNNNNNCGW 2 cut(s) 122, 167
PdiI GCCGGC 1 cut(s) 168
PfeI GAWTC 3 cut(s) 343, 540, 589
PfoI TCCNGGA 1 cut(s) 424
PkrI GCNGC 3 cut(s) 53, 440, 443
PleI GAGTC 3 cut(s) 474, 557, 642
PpsI GAGTC 3 cut(s) 474, 557, 642
PshAI GACNNNNGTC 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 152
Psp6I CCWGG 2 cut(s) 186, 456
PspFI CCCAGC 1 cut(s) 641
PspGI CCWGG 2 cut(s) 186, 456
PspN4I GGNNCC 1 cut(s) 629
PspPI GGNCC 5 cut(s) 39, 53, 190, 374, 639
PsrI GAACNNNNNNTAC 2 cut(s) 284, 316
PstI CTGCAG 1 cut(s) 443
PsuI RGATCY 3 cut(s) 111, 239, 479
RsaI GTAC 2 cut(s) 293, 537
RsaNI GTAC 2 cut(s) 292, 536
SacI GAGCTC 1 cut(s) 152
SatI GCNGC 3 cut(s) 52, 439, 442
Sau3AI GATC 7 cut(s) 111, 213, 239, 321, 347, 428, 479
Sau96I GGNCC 5 cut(s) 39, 53, 190, 374, 639
SchI GAGTC 3 cut(s) 475, 558, 642
ScrFI CCNGG 6 cut(s) 37, 38, 188, 426, 458, 625
SduI GDGCHC 3 cut(s) 63, 152, 410
SfaNI GCATC 3 cut(s) 20, 375, 430
SfcI CTRYAG 1 cut(s) 439
SfuI TTCGAA 1 cut(s) 341
SgrAI CRCCGGYG 1 cut(s) 166
SinI GGWCC 2 cut(s) 190, 374
SmaI CCCGGG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
SsiI CCGC 7 cut(s) 9, 51, 96, 108, 243, 582, 616
SspMI CTAG 1 cut(s) 676
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 6 cut(s) 35, 36, 186, 424, 456, 623
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 2 cut(s) 297, 419
TaqI TCGA 9 cut(s) 23, 143, 309, 320, 341, 346, 383, 491, 552
TaqII GACCGA 1 cut(s) 391
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 3 cut(s) 343, 540, 589
TscAI CASTG 1 cut(s) 550
TseI GCWGC 2 cut(s) 438, 441
TspGWI ACGGA 1 cut(s) 273
TspMI CCCGGG 1 cut(s) 36
TspRI CASTG 1 cut(s) 550
VpaK11BI GGWCC 2 cut(s) 190, 374
XagI CCTNNNNNAGG 1 cut(s) 22
XmaI CCCGGG 1 cut(s) 36
XspI CTAG 1 cut(s) 676
ZraI GACGTC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.