MD01G1002300.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014643
Physical Location & Seq
Forward (+)
949449 .. 949940
492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1002300.v1.1.491

Sequence Viewer

Length: 492 bp
ATGATCCACCTGCTTCACATGCCCAACAGAAGCTTCGCATCGGTGAGGACATTCTTGGATCATATGAGAAAACTATTACCTAAGATTGTTGTCCTAGTAGAAGAAGAGCTGTTGAATTTTTCGAGAGTATCTTCAATGTCCTTCGTAGAGTTCTTCTGCGAGGCTGTCCACCATTACACTGTGCTTTCTGACTCATTCGGGACTAGTTTTCGGGGTTTAGGGTACAAAACCGGATTGAAACAGATAGAGGAGGAGAGTTTGGGGATTAAGATTGTCGATAGTGCGATGCGGCTCCCGTGTGACAAAGAGGAAAGGATGTTGATGTGGGGGGATGGTTTTTTTGCCTCCTTGAAGGGCGGTTTTAAACCCATCCCGTTCACTTGTTACAATATTCCTCTGGCCAACTACTTGGCCAACCTGTTCGATAAGGGTTACTCTGTTCAGCATCACAATTGCTGGTTAGTTTGTGTTGGAAATCAAGGCCTATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

164

Amino Acids

18.6

Weight (kDa)

7.03

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 3 - 155 1.5e-13 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 18
Acc36I ACCTGC 1 cut(s) 18
AciI CCGC 2 cut(s) 289, 357
AclWI GGATC 1 cut(s) 66
AcoI YGGCCR 2 cut(s) 399, 411
AcsI RAATTY 1 cut(s) 115
AfaI GTAC 1 cut(s) 224
AgsI TTSAA 4 cut(s) 115, 135, 238, 352
AhlI ACTAGT 1 cut(s) 203
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 2 cut(s) 33, 109
AluI AGCT 2 cut(s) 33, 109
AlwI GGATC 1 cut(s) 66
AoxI GGCC 3 cut(s) 399, 411, 481
ApoI RAATTY 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 55
BalI TGGCCA 2 cut(s) 401, 413
BccI CCATC 2 cut(s) 326, 377
BcuI ACTAGT 1 cut(s) 203
BfaI CTAG 2 cut(s) 95, 204
BfuAI ACCTGC 1 cut(s) 18
BisI GCNGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 291
BmiI GGNNCC 1 cut(s) 293
BmsI GCATC 3 cut(s) 47, 276, 454
BsaWI WCCGGW 1 cut(s) 230
BseGI GGATG 3 cut(s) 321, 337, 369
BseRI GAGGAG 2 cut(s) 263, 266
BshFI GGCC 3 cut(s) 401, 413, 483
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 214
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 3 cut(s) 401, 413, 483
Bsp143I GATC 2 cut(s) 3, 58
BspACI CCGC 2 cut(s) 289, 357
BspANI GGCC 3 cut(s) 401, 413, 483
BspLI GGNNCC 1 cut(s) 293
BspMI ACCTGC 1 cut(s) 18
BspPI GGATC 1 cut(s) 66
BspQI GCTCTTC 1 cut(s) 99
BssMI GATC 2 cut(s) 3, 58
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 81
BstF5I GGATG 3 cut(s) 321, 337, 369
BstKTI GATC 2 cut(s) 6, 61
BstMBI GATC 2 cut(s) 3, 58
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstNSI RCATGY 1 cut(s) 22
BstXI CCANNNNNNTGG 1 cut(s) 409
BsuRI GGCC 3 cut(s) 401, 413, 483
BtgZI GCGATG 1 cut(s) 299
BtsCI GGATG 3 cut(s) 321, 337, 369
BtsIMutI CAGTG 1 cut(s) 177
BveI ACCTGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 223
CviAII CATG 1 cut(s) 19
CviJI RGCY 7 cut(s) 33, 109, 164, 292, 401, 413, 483
CviKI_1 RGCY 7 cut(s) 33, 109, 164, 292, 401, 413, 483
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 81
DpnI GATC 2 cut(s) 5, 60
DpnII GATC 2 cut(s) 3, 58
DraI TTTAAA 1 cut(s) 364
EaeI YGGCCR 2 cut(s) 399, 411
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 483
FaeI CATG 1 cut(s) 22
FaiI YATR 4 cut(s) 20, 63, 65, 487
FaqI GGGAC 1 cut(s) 214
FatI CATG 1 cut(s) 18
FauNDI CATATG 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 290
FokI GGATG 3 cut(s) 328, 344, 356
Fsp4HI GCNGC 1 cut(s) 290
FspBI CTAG 2 cut(s) 95, 204
GluI GCNGC 1 cut(s) 290
HaeIII GGCC 3 cut(s) 401, 413, 483
HapII CCGG 1 cut(s) 231
Hin1II CATG 1 cut(s) 22
HindIII AAGCTT 1 cut(s) 31
HinfI GANTC 1 cut(s) 191
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 2 cut(s) 169, 378
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 2 cut(s) 123, 199
Hpy8I GTNNAC 2 cut(s) 169, 378
HpyAV CCTTC 2 cut(s) 151, 346
HpyCH4III ACNGT 1 cut(s) 181
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpyF3I CTNAG 1 cut(s) 81
Hsp92II CATG 1 cut(s) 22
Kzo9I GATC 2 cut(s) 3, 58
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 297
LpnPI CCDG 5 cut(s) 23, 244, 383, 431, 442
LweI GCATC 3 cut(s) 47, 276, 454
MaeI CTAG 2 cut(s) 95, 204
MaeIII GTNAC 3 cut(s) 299, 383, 431
MalI GATC 2 cut(s) 5, 60
MboI GATC 2 cut(s) 3, 58
MboII GAAGA 4 cut(s) 113, 116, 123, 145
MfeI CAATTG 1 cut(s) 451
MlsI TGGCCA 2 cut(s) 401, 413
MluCI AATT 2 cut(s) 115, 451
MluNI TGGCCA 2 cut(s) 401, 413
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 451
MnlI CCTC 7 cut(s) 39, 154, 241, 244, 301, 355, 405
Mox20I TGGCCA 2 cut(s) 401, 413
MscI TGGCCA 2 cut(s) 401, 413
MseI TTAA 3 cut(s) 267, 363, 490
Msp20I TGGCCA 2 cut(s) 401, 413
MspI CCGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 451
MwoI GCNNNNNNNGC 1 cut(s) 19
NdeI CATATG 1 cut(s) 63
NdeII GATC 2 cut(s) 3, 58
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 293
NmuCI GTSAC 1 cut(s) 299
NspI RCATGY 1 cut(s) 22
PaqCI CACCTGC 1 cut(s) 18
PceI AGGCCT 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 99
PkrI GCNGC 1 cut(s) 291
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 293
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 3 cut(s) 267, 363, 490
SatI GCNGC 1 cut(s) 290
Sau3AI GATC 2 cut(s) 3, 58
SchI GAGTC 1 cut(s) 185
SetI ASST 5 cut(s) 12, 35, 82, 111, 420
SfaNI GCATC 3 cut(s) 47, 276, 454
SpeI ACTAGT 1 cut(s) 203
Sse9I AATT 2 cut(s) 115, 451
SseBI AGGCCT 1 cut(s) 483
SsiI CCGC 2 cut(s) 289, 357
SspI AATATT 1 cut(s) 391
SspMI CTAG 2 cut(s) 95, 204
StuI AGGCCT 1 cut(s) 483
TaaI ACNGT 1 cut(s) 181
TaqI TCGA 3 cut(s) 122, 276, 423
TasI AATT 2 cut(s) 115, 451
TauI GCSGC 1 cut(s) 292
Tru1I TTAA 3 cut(s) 267, 363, 490
Tru9I TTAA 3 cut(s) 267, 363, 490
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 299
Tsp45I GTSAC 1 cut(s) 299
TspRI CASTG 1 cut(s) 184
XapI RAATTY 1 cut(s) 115
XceI RCATGY 1 cut(s) 22
XspI CTAG 2 cut(s) 95, 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.