Rh2DG282800

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014643
Physical Location & Seq
Reverse (-)
32310597 .. 32311550
954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG282800.1

Sequence Viewer

Length: 954 bp
ATGGAAGTTGAGCTCATTGATCACCCATTCAACCCCAGCTACGACCTCTATGACTGTAGCTTTGAATCTGATTCTTCCGTCCAAACATCAGATTTATTTACTCCTTGTACCACCAATATTGTCGATCAAAACTCGAACCACGAGTGCCTCAACAGATTGCGCCAAATAGAATCACAGCTCATGGACATAGACTCGTCTATCAGCAGTGGAAGTGATGTAATCGGTCTTGATGGAGTTGTTGATAGTGAATTCGCTTCTTACATAAGTGAAGAAGCACCAGAAAATGTCCATGTTTCGACAGAAGACAATTCCCTTTTGAAGGAGATTCAGATAGATTTGTTGGAAGAGAGCTGCTTAACTGAACTTTTATTCGCAGGAGCTGAAGCTGTTGAGGCACAGAACTGGGTTTTTGCTTCAAATATAATCGCAAAGCTCAACACTCTTCTATCCGGCAGCGAAAATGGAGACAACTCGTTCAACAAATTGGCCACGTTCTTCACTCAAGGCCTTCATTACAAGACCATTAACTCTATTGAAGTTCCCCATGAGCTAGCTGTTTCTAGAAACACAGACACAATGTCTTCATTTCAGATGCTCCAAGAGCTCTCTCCTTTTGTTAAGTTTGCGCATTTCACTGCCAACCAAGCGATTCTCGAGGCCACACAAGAGGATGAGGACATTCATGTCATTGACTTTGACATCATGGAGGGTAGCCAGTGGCCTTCATTGATGGTTGACCTTGCAATGAGGAAAGGTCGCCGCTGTGCTTCTCTCAAGGTAACCGCCATCACAGTTGACCAGCAAAGCGCCGTTCTTGCTCAACAAACTGGAAGAAGACTTAAAGAGTTCTCAGAGTCTATCAATTTGTTATTTATTTTTGATCAGATGATGATGGCCACAGAAGAAGATTTTGCTAAAATCGAAGTTGTTGCTGATGCAGTACGTAATAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

317

Amino Acids

35.38

Weight (kDa)

4.22

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 120 - 303 1.8e-44 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 683
Acc16I TGCGCA 1 cut(s) 627
AciI CCGC 2 cut(s) 760, 783
AcoI YGGCCR 2 cut(s) 486, 894
AcsI RAATTY 1 cut(s) 248
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 2 cut(s) 109, 942
AfiI CCNNNNNNNGG 1 cut(s) 319
AgsI TTSAA 6 cut(s) 31, 65, 319, 417, 478, 536
AhdI GACNNNNNGTC 1 cut(s) 577
Alw21I GWGCWC 2 cut(s) 15, 606
Alw26I GTCTC 1 cut(s) 459
AlwNI CAGNNNCTG 1 cut(s) 380
Ama87I CYCGRG 1 cut(s) 653
AoxI GGCC 5 cut(s) 486, 505, 657, 719, 894
ApeKI GCWGC 2 cut(s) 351, 453
ApoI RAATTY 1 cut(s) 248
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 3 cut(s) 162, 628, 809
AsuHPI GGTGA 1 cut(s) 14
AsuNHI GCTAGC 1 cut(s) 550
AvaI CYCGRG 1 cut(s) 653
BalI TGGCCA 2 cut(s) 488, 896
BanII GRGCYC 2 cut(s) 15, 606
BauI CACGAG 1 cut(s) 140
BbsI GAAGAC 3 cut(s) 309, 573, 841
Bbv12I GWGCWC 2 cut(s) 15, 606
BbvI GCAGC 2 cut(s) 338, 465
BccI CCATC 4 cut(s) 224, 724, 794, 886
BceAI ACGGC 1 cut(s) 794
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BclI TGATCA 2 cut(s) 19, 880
BcoDI GTCTC 1 cut(s) 459
BfaI CTAG 2 cut(s) 551, 561
BfmI CTRYAG 1 cut(s) 55
BfoI RGCGCY 1 cut(s) 810
BisI GCNGC 3 cut(s) 352, 454, 760
BlsI GCNGC 3 cut(s) 353, 455, 761
BmeRI GACNNNNNGTC 1 cut(s) 577
BmeT110I CYCGRG 1 cut(s) 653
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 2 cut(s) 582, 925
BmtI GCTAGC 1 cut(s) 554
BmuI ACTGGG 1 cut(s) 412
BpiI GAAGAC 3 cut(s) 309, 573, 841
BpuEI CTTGAG 2 cut(s) 486, 758
BsaAI YACGTR 1 cut(s) 944
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse1I ACTGG 3 cut(s) 407, 715, 832
Bse3DI GCAATG 1 cut(s) 750
BseGI GGATG 1 cut(s) 676
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 1 cut(s) 864
BseNI ACTGG 3 cut(s) 407, 715, 832
BseXI GCAGC 2 cut(s) 338, 465
BseYI CCCAGC 1 cut(s) 35
BshFI GGCC 5 cut(s) 488, 507, 659, 721, 896
BsiHKAI GWGCWC 2 cut(s) 15, 606
BsiHKCI CYCGRG 1 cut(s) 653
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 1 cut(s) 459
BsnI GGCC 5 cut(s) 488, 507, 659, 721, 896
BsoBI CYCGRG 1 cut(s) 653
Bsp1286I GDGCHC 2 cut(s) 15, 606
Bsp143I GATC 3 cut(s) 19, 124, 880
BspACI CCGC 2 cut(s) 760, 783
BspANI GGCC 5 cut(s) 488, 507, 659, 721, 896
BspCNI CTCAG 1 cut(s) 863
BspOI GCTAGC 1 cut(s) 554
BsrDI GCAATG 1 cut(s) 750
BsrI ACTGG 3 cut(s) 407, 715, 832
BssMI GATC 3 cut(s) 19, 124, 880
BssSI CACGAG 1 cut(s) 140
Bst2BI CACGAG 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 56, 793
Bst6I CTCTTC 2 cut(s) 339, 447
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 1 cut(s) 850
BstEII GGTNACC 1 cut(s) 778
BstENI CCTNNNNNAGG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 676
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 3 cut(s) 162, 628, 809
BstKTI GATC 3 cut(s) 22, 127, 883
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 3 cut(s) 19, 124, 880
BstMWI GCNNNNNNNGC 4 cut(s) 392, 601, 644, 815
BstPI GGTNACC 1 cut(s) 778
BstSFI CTRYAG 1 cut(s) 55
BstSNI TACGTA 1 cut(s) 944
BstV1I GCAGC 2 cut(s) 338, 465
BstV2I GAAGAC 3 cut(s) 309, 573, 841
BsuRI GGCC 5 cut(s) 488, 507, 659, 721, 896
BtsCI GGATG 1 cut(s) 676
BtsI GCAGTG 2 cut(s) 211, 633
BtsIMutI CAGTG 3 cut(s) 211, 633, 722
Cac8I GCNNGC 1 cut(s) 552
CaiI CAGNNNCTG 1 cut(s) 380
CfoI GCGC 3 cut(s) 162, 628, 809
Csp6I GTAC 2 cut(s) 108, 941
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 5 cut(s) 181, 290, 545, 683, 703
CviQI GTAC 2 cut(s) 108, 941
DdeI CTNAG 1 cut(s) 850
DpnI GATC 3 cut(s) 21, 126, 882
DpnII GATC 3 cut(s) 19, 124, 880
DrdI GACNNNNNNGTC 1 cut(s) 683
DriI GACNNNNNGTC 1 cut(s) 577
DseDI GACNNNNNNGTC 1 cut(s) 683
EaeI YGGCCR 2 cut(s) 486, 894
Eam1104I CTCTTC 2 cut(s) 339, 447
Eam1105I GACNNNNNGTC 1 cut(s) 577
EarI CTCTTC 2 cut(s) 339, 447
Ecl136II GAGCTC 2 cut(s) 13, 604
Eco105I TACGTA 1 cut(s) 944
Eco147I AGGCCT 1 cut(s) 507
Eco24I GRGCYC 2 cut(s) 15, 606
Eco53kI GAGCTC 2 cut(s) 13, 604
Eco57I CTGAAG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 653
Eco91I GGTNACC 1 cut(s) 778
EcoICRI GAGCTC 2 cut(s) 13, 604
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoO65I GGTNACC 1 cut(s) 778
EcoRI GAATTC 1 cut(s) 248
EcoT38I GRGCYC 2 cut(s) 15, 606
FaeI CATG 5 cut(s) 184, 293, 548, 686, 706
FaiI YATR 9 cut(s) 51, 182, 188, 263, 291, 422, 546, 684, 704
FatI CATG 5 cut(s) 180, 289, 544, 682, 702
FbaI TGATCA 2 cut(s) 19, 880
Fnu4HI GCNGC 3 cut(s) 352, 454, 760
FokI GGATG 1 cut(s) 683
FriOI GRGCYC 2 cut(s) 15, 606
Fsp4HI GCNGC 3 cut(s) 352, 454, 760
FspBI CTAG 2 cut(s) 551, 561
FspI TGCGCA 1 cut(s) 627
GlaI GCGC 3 cut(s) 161, 627, 808
GluI GCNGC 3 cut(s) 352, 454, 760
GsaI CCCAGC 1 cut(s) 39
HaeII RGCGCY 1 cut(s) 810
HaeIII GGCC 5 cut(s) 488, 507, 659, 721, 896
HapII CCGG 1 cut(s) 450
HhaI GCGC 3 cut(s) 162, 628, 809
Hin1II CATG 5 cut(s) 184, 293, 548, 686, 706
Hin6I GCGC 3 cut(s) 160, 626, 807
HinP1I GCGC 3 cut(s) 160, 626, 807
HincII GTYRAC 2 cut(s) 736, 796
HindII GTYRAC 2 cut(s) 736, 796
HinfI GANTC 7 cut(s) 65, 71, 170, 191, 325, 649, 854
HpaII CCGG 1 cut(s) 450
HphI GGTGA 1 cut(s) 14
Hpy166II GTNNAC 2 cut(s) 736, 796
Hpy188I TCNGA 6 cut(s) 70, 91, 330, 591, 853, 885
Hpy188III TCNNGA 3 cut(s) 227, 561, 653
Hpy8I GTNNAC 2 cut(s) 736, 796
HpyAV CCTTC 3 cut(s) 313, 518, 732
HpyCH4III ACNGT 2 cut(s) 56, 793
HpyCH4IV ACGT 2 cut(s) 491, 943
HpyCH4V TGCA 2 cut(s) 743, 938
HpyF10VI GCNNNNNNNGC 4 cut(s) 392, 601, 644, 815
HpyF3I CTNAG 1 cut(s) 850
HpySE526I ACGT 2 cut(s) 491, 943
Hsp92II CATG 5 cut(s) 184, 293, 548, 686, 706
HspAI GCGC 3 cut(s) 160, 626, 807
Ksp22I TGATCA 2 cut(s) 19, 880
Kzo9I GATC 3 cut(s) 19, 124, 880
LmnI GCTCC 2 cut(s) 377, 600
LpnPI CCDG 8 cut(s) 49, 291, 360, 388, 463, 728, 812, 813
Lsp1109I GCAGC 2 cut(s) 338, 465
LweI GCATC 2 cut(s) 582, 925
MaeI CTAG 2 cut(s) 551, 561
MaeII ACGT 2 cut(s) 491, 943
MaeIII GTNAC 1 cut(s) 778
MalI GATC 3 cut(s) 21, 126, 882
MboI GATC 3 cut(s) 19, 124, 880
MhlI GDGCHC 2 cut(s) 15, 606
MlsI TGGCCA 2 cut(s) 488, 896
MluCI AATT 4 cut(s) 248, 307, 482, 862
MluNI TGGCCA 2 cut(s) 488, 896
MlyI GAGTC 2 cut(s) 185, 863
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 8 cut(s) 56, 158, 385, 649, 661, 667, 700, 741
Mox20I TGGCCA 2 cut(s) 488, 896
MscI TGGCCA 2 cut(s) 488, 896
MseI TTAA 4 cut(s) 356, 525, 618, 840
Msp20I TGGCCA 2 cut(s) 488, 896
MspA1I CMGCKG 1 cut(s) 762
MspI CCGG 1 cut(s) 450
MwoI GCNNNNNNNGC 4 cut(s) 392, 601, 644, 815
NdeII GATC 3 cut(s) 19, 124, 880
NheI GCTAGC 1 cut(s) 550
NlaIII CATG 5 cut(s) 184, 293, 548, 686, 706
NsbI TGCGCA 1 cut(s) 627
PaeR7I CTCGAG 1 cut(s) 653
PceI AGGCCT 1 cut(s) 507
PfeI GAWTC 5 cut(s) 65, 71, 170, 325, 649
PkrI GCNGC 3 cut(s) 353, 455, 761
PleI GAGTC 2 cut(s) 185, 862
PpsI GAGTC 2 cut(s) 185, 862
Ppu21I YACGTR 1 cut(s) 944
Psp124BI GAGCTC 2 cut(s) 15, 606
PspEI GGTNACC 1 cut(s) 778
PspFI CCCAGC 1 cut(s) 35
PstNI CAGNNNCTG 1 cut(s) 380
RsaI GTAC 2 cut(s) 109, 942
RsaNI GTAC 2 cut(s) 108, 941
SacI GAGCTC 2 cut(s) 15, 606
SaqAI TTAA 4 cut(s) 356, 525, 618, 840
SatI GCNGC 3 cut(s) 352, 454, 760
Sau3AI GATC 3 cut(s) 19, 124, 880
SchI GAGTC 2 cut(s) 185, 863
SduI GDGCHC 2 cut(s) 15, 606
SfaNI GCATC 2 cut(s) 582, 925
SfcI CTRYAG 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 653
SlaI CTCGAG 1 cut(s) 653
SmlI CTYRAG 3 cut(s) 501, 653, 773
SmoI CTYRAG 3 cut(s) 501, 653, 773
SnaBI TACGTA 1 cut(s) 944
Sse9I AATT 4 cut(s) 248, 307, 482, 862
SseBI AGGCCT 1 cut(s) 507
SsiI CCGC 2 cut(s) 760, 783
SspI AATATT 1 cut(s) 118
SspMI CTAG 2 cut(s) 551, 561
SstI GAGCTC 2 cut(s) 15, 606
StuI AGGCCT 1 cut(s) 507
TaaI ACNGT 2 cut(s) 56, 793
TaiI ACGT 2 cut(s) 494, 946
TaqI TCGA 5 cut(s) 123, 134, 296, 654, 921
TaqII GACCGA 1 cut(s) 212
TasI AATT 4 cut(s) 248, 307, 482, 862
TauI GCSGC 1 cut(s) 762
TfiI GAWTC 5 cut(s) 65, 71, 170, 325, 649
Tru1I TTAA 4 cut(s) 356, 525, 618, 840
Tru9I TTAA 4 cut(s) 356, 525, 618, 840
TscAI CASTG 3 cut(s) 211, 640, 722
TseI GCWGC 2 cut(s) 351, 453
TspDTI ATGAA 4 cut(s) 500, 573, 671, 714
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 3 cut(s) 211, 640, 722
XagI CCTNNNNNAGG 1 cut(s) 317
XapI RAATTY 1 cut(s) 248
XbaI TCTAGA 1 cut(s) 560
XhoI CTCGAG 1 cut(s) 653
XspI CTAG 2 cut(s) 551, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.