Rh2DG301900

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014643
Physical Location & Seq
Reverse (-)
36011581 .. 36013671
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG301900.1

Sequence Viewer

Length: 774 bp
ATGGAAGTTGAGCTCATTGATCACCCATTCAACCCGAGCTTCGATCTCTATGACTGTAGCTTTGAATCTGATTCTTCCTTCCAAACATCAGATTTATTTACTCGTACCACCAATGTTGATCAAAACTCGGACCACGAGTGCCTCAACAGAATGCTCCAAATAGAATCACAGCTCATGGACATAGACTCGTCTATCAGCAGTGGAAGTGATGTAATCGGTCTTGATGGTGTTGTTGATAGTGAATTCGCTTCTTACATAAGTGAAGAAGCACCAGAAAATGTCCGTGTTTCGACAGAAGGCAATTCCCTTTTGAAGGAGATTCAGATAGATTTGCTGGAAGAGAGCAGCTTAACTGAACTTCTATTCGCAGGAGCTGAAGCGGTTGAATCACAGAACTGGGTTTTTGCTTCAAATATAATCGCAAAGCTCAACACTCTTCTATCCGGTAGCGAAAATGGAGACAACTCGTTCAACAAATTGGCCACGTTCTTCACTAAAGGCCTTCATTACAAGACCATTAACTCTATTGAAGTTCCCCATGAGCCAGCTGTTTCTAGAAACACAGAAACAATGTCTTCATTTCAGATGCTCCTAGAGCTCTCTCCTTTTGTTAAGTTTGCGCATTTCACAGCCAACCAAGCGATTCTCGAGGCCACACAAGAGGATGAGGACATTCATGTCATTGACTTTGACATCATGGAGGGTAGCCAGTGGCCTTCATTGATGGTTGATCTTGCAAGGAGGAAAGGTCGCCGCTGTGCTTCTCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

257

Amino Acids

28.71

Weight (kDa)

4.28

Isoelectric Point (pI)

53.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 118 - 251 8.8e-35 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 677
Acc16I TGCGCA 1 cut(s) 621
AciI CCGC 2 cut(s) 380, 754
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 1 cut(s) 313
AgsI TTSAA 7 cut(s) 31, 65, 313, 386, 411, 472, 530
AluBI AGCT 9 cut(s) 13, 39, 60, 172, 348, 374, 427, 548, 598
AluI AGCT 9 cut(s) 13, 39, 60, 172, 348, 374, 427, 548, 598
Alw21I GWGCWC 2 cut(s) 15, 600
Alw26I GTCTC 1 cut(s) 453
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 2 cut(s) 34, 647
AoxI GGCC 4 cut(s) 480, 499, 651, 713
ApeKI GCWGC 1 cut(s) 345
ApoI RAATTY 1 cut(s) 242
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 14
AvaI CYCGRG 2 cut(s) 34, 647
AvaII GGWCC 1 cut(s) 130
BalI TGGCCA 1 cut(s) 482
BanII GRGCYC 2 cut(s) 15, 600
BauI CACGAG 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 2 cut(s) 15, 600
BbvI GCAGC 1 cut(s) 357
BccI CCATC 2 cut(s) 218, 718
BclI TGATCA 2 cut(s) 19, 118
BcoDI GTCTC 1 cut(s) 453
BfaI CTAG 2 cut(s) 555, 593
BfmI CTRYAG 1 cut(s) 55
BisI GCNGC 2 cut(s) 346, 754
BlsI GCNGC 2 cut(s) 347, 755
Bme18I GGWCC 1 cut(s) 130
BmeT110I CYCGRG 2 cut(s) 34, 647
BmgT120I GGNCC 1 cut(s) 130
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 1 cut(s) 576
BmuI ACTGGG 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 752
BsaWI WCCGGW 1 cut(s) 443
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 2 cut(s) 401, 709
BseGI GGATG 1 cut(s) 670
BseLI CCNNNNNNNGG 1 cut(s) 313
BseNI ACTGG 2 cut(s) 401, 709
BseXI GCAGC 1 cut(s) 357
BshFI GGCC 4 cut(s) 482, 501, 653, 715
BsiHKAI GWGCWC 2 cut(s) 15, 600
BsiHKCI CYCGRG 2 cut(s) 34, 647
BsiSI CCGG 1 cut(s) 444
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 1 cut(s) 453
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 4 cut(s) 482, 501, 653, 715
BsoBI CYCGRG 2 cut(s) 34, 647
Bsp1286I GDGCHC 2 cut(s) 15, 600
Bsp143I GATC 4 cut(s) 19, 43, 118, 730
BspACI CCGC 2 cut(s) 380, 754
BspANI GGCC 4 cut(s) 482, 501, 653, 715
BsrI ACTGG 2 cut(s) 401, 709
BssMI GATC 4 cut(s) 19, 43, 118, 730
BssSI CACGAG 1 cut(s) 134
Bst2BI CACGAG 1 cut(s) 134
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 2 cut(s) 333, 441
BstC8I GCNNGC 1 cut(s) 546
BstENI CCTNNNNNAGG 1 cut(s) 311
BstF5I GGATG 1 cut(s) 670
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 4 cut(s) 22, 46, 121, 733
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 4 cut(s) 19, 43, 118, 730
BstMWI GCNNNNNNNGC 2 cut(s) 595, 638
BstSFI CTRYAG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 357
BstV2I GAAGAC 1 cut(s) 567
BsuRI GGCC 4 cut(s) 482, 501, 653, 715
BtsCI GGATG 1 cut(s) 670
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 2 cut(s) 205, 716
Cac8I GCNNGC 1 cut(s) 546
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 1 cut(s) 622
Cfr13I GGNCC 1 cut(s) 130
Csp6I GTAC 1 cut(s) 105
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 4 cut(s) 175, 539, 677, 697
CviQI GTAC 1 cut(s) 105
DpnI GATC 4 cut(s) 21, 45, 120, 732
DpnII GATC 4 cut(s) 19, 43, 118, 730
DrdI GACNNNNNNGTC 1 cut(s) 677
DseDI GACNNNNNNGTC 1 cut(s) 677
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 2 cut(s) 333, 441
EarI CTCTTC 2 cut(s) 333, 441
Ecl136II GAGCTC 2 cut(s) 13, 598
Eco147I AGGCCT 1 cut(s) 501
Eco24I GRGCYC 2 cut(s) 15, 600
Eco47I GGWCC 1 cut(s) 130
Eco53kI GAGCTC 2 cut(s) 13, 598
Eco57I CTGAAG 1 cut(s) 396
Eco88I CYCGRG 2 cut(s) 34, 647
EcoICRI GAGCTC 2 cut(s) 13, 598
EcoNI CCTNNNNNAGG 1 cut(s) 311
EcoRI GAATTC 1 cut(s) 242
EcoT38I GRGCYC 2 cut(s) 15, 600
FaeI CATG 4 cut(s) 178, 542, 680, 700
FaiI YATR 8 cut(s) 51, 176, 182, 257, 416, 540, 678, 698
FatI CATG 4 cut(s) 174, 538, 676, 696
FbaI TGATCA 2 cut(s) 19, 118
Fnu4HI GCNGC 2 cut(s) 346, 754
FokI GGATG 1 cut(s) 677
FriOI GRGCYC 2 cut(s) 15, 600
Fsp4HI GCNGC 2 cut(s) 346, 754
FspBI CTAG 2 cut(s) 555, 593
FspI TGCGCA 1 cut(s) 621
GlaI GCGC 1 cut(s) 621
GluI GCNGC 2 cut(s) 346, 754
HaeIII GGCC 4 cut(s) 482, 501, 653, 715
HapII CCGG 1 cut(s) 444
HhaI GCGC 1 cut(s) 622
Hin1II CATG 4 cut(s) 178, 542, 680, 700
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HinfI GANTC 7 cut(s) 65, 71, 164, 185, 319, 386, 643
HpaII CCGG 1 cut(s) 444
HphI GGTGA 1 cut(s) 14
Hpy188I TCNGA 5 cut(s) 70, 91, 130, 324, 585
Hpy188III TCNNGA 3 cut(s) 221, 555, 647
HpyAV CCTTC 5 cut(s) 88, 290, 307, 512, 726
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 1 cut(s) 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 595, 638
HpySE526I ACGT 1 cut(s) 485
Hsp92II CATG 4 cut(s) 178, 542, 680, 700
HspAI GCGC 1 cut(s) 620
Ksp22I TGATCA 2 cut(s) 19, 118
Kzo9I GATC 4 cut(s) 19, 43, 118, 730
LmnI GCTCC 3 cut(s) 159, 371, 594
LpnPI CCDG 7 cut(s) 285, 320, 354, 382, 457, 558, 722
Lsp1109I GCAGC 1 cut(s) 357
LweI GCATC 1 cut(s) 576
MaeI CTAG 2 cut(s) 555, 593
MaeII ACGT 1 cut(s) 485
MalI GATC 4 cut(s) 21, 45, 120, 732
MboI GATC 4 cut(s) 19, 43, 118, 730
MboII GAAGA 6 cut(s) 66, 275, 350, 428, 481, 567
MhlI GDGCHC 2 cut(s) 15, 600
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 3 cut(s) 242, 301, 476
MluNI TGGCCA 1 cut(s) 482
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 6 cut(s) 152, 643, 655, 661, 694, 735
Mox20I TGGCCA 1 cut(s) 482
MscI TGGCCA 1 cut(s) 482
MseI TTAA 3 cut(s) 350, 519, 612
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 2 cut(s) 548, 756
MspI CCGG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 156
MwoI GCNNNNNNNGC 2 cut(s) 595, 638
NdeII GATC 4 cut(s) 19, 43, 118, 730
NlaIII CATG 4 cut(s) 178, 542, 680, 700
NsbI TGCGCA 1 cut(s) 621
PaeR7I CTCGAG 1 cut(s) 647
PceI AGGCCT 1 cut(s) 501
PctI GAATGC 1 cut(s) 156
PfeI GAWTC 6 cut(s) 65, 71, 164, 319, 386, 643
PkrI GCNGC 2 cut(s) 347, 755
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Psp124BI GAGCTC 2 cut(s) 15, 600
PspPI GGNCC 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
SacI GAGCTC 2 cut(s) 15, 600
SaqAI TTAA 3 cut(s) 350, 519, 612
SatI GCNGC 2 cut(s) 346, 754
Sau3AI GATC 4 cut(s) 19, 43, 118, 730
Sau96I GGNCC 1 cut(s) 130
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 2 cut(s) 15, 600
SfaNI GCATC 1 cut(s) 576
SfcI CTRYAG 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 647
SinI GGWCC 1 cut(s) 130
SlaI CTCGAG 1 cut(s) 647
SmlI CTYRAG 2 cut(s) 647, 767
SmoI CTYRAG 2 cut(s) 647, 767
Sse9I AATT 3 cut(s) 242, 301, 476
SseBI AGGCCT 1 cut(s) 501
SsiI CCGC 2 cut(s) 380, 754
SspMI CTAG 2 cut(s) 555, 593
SstI GAGCTC 2 cut(s) 15, 600
StuI AGGCCT 1 cut(s) 501
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 1 cut(s) 488
TaqI TCGA 3 cut(s) 42, 290, 648
TaqII GACCGA 1 cut(s) 206
TasI AATT 3 cut(s) 242, 301, 476
TauI GCSGC 1 cut(s) 756
TfiI GAWTC 6 cut(s) 65, 71, 164, 319, 386, 643
Tru1I TTAA 3 cut(s) 350, 519, 612
Tru9I TTAA 3 cut(s) 350, 519, 612
TscAI CASTG 2 cut(s) 205, 716
TseI GCWGC 1 cut(s) 345
TspDTI ATGAA 4 cut(s) 494, 567, 665, 708
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 2 cut(s) 205, 716
VpaK11BI GGWCC 1 cut(s) 130
XagI CCTNNNNNAGG 1 cut(s) 311
XapI RAATTY 1 cut(s) 242
XbaI TCTAGA 1 cut(s) 554
XhoI CTCGAG 1 cut(s) 647
XspI CTAG 2 cut(s) 555, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.