Rh2AG275700

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014643
Physical Location & Seq
Reverse (-)
32991521 .. 32992399
879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG275700.1

Sequence Viewer

Length: 879 bp
ATGGAAGTTGAGCTCATTGATCACCCATTCAACCCGAGCTACGATCTCTATGACTGTAGCTTTGAATCTGATTCTTCCTTCCAAACATCAGATTTATTTACTCCTTGTGCCACCAATGTTGATCAAAACTCGGACCACGAGTGCCTCAACAGATTGCTCCAAATAGAATCACAGCTCATGGACATAGACTCGTCTATCAGCAGTGGAAGTGATGTAATCGGTCTTGATGGTGTTGTTGATAGTGAATTCGTTTCTTACATAAGTGAAGAAGCACCAGAAAATGTCCATGTTTCGACAGAAGGCAATTCCCTTTTGAAGGAGATTCAGATAGATTTGCTGGAAGAGAGCAGCTTAACTGAACTTTTATTCGCAGGAGCTGAAGCTGTTGAAGCACAGAACTGGGTTTTTGCTTCAAATATAATCGCAAAGCTCAACTCTCTTCTATCCGGTAGCGAAAATGAAGACAACTCGTTCAACAAATTGGCCACGTTCTTCACTCAAGGCCTTCATTACAAGACCATTAACTCTATTGAAGTTCCCCATGAGCCAGCTGTTTCTAGAAACACAGACACAATGTCTTCATTTCAGATGCTCCAAGAGCTCTCTCCTTTTGTTAAGTTTGCTCATTTCACAGCCAACCAAGCGATTCTCGAGGCCACGCAAGAGGATGGGGACATTCATGTCATTGACTTTGACATCATGGAGGGTAGCCAGTGGCCTTCATTGATGGTTGACCTTGCAGTGAGGAAAGGTCGCCGCTGTGCTTCTCTCAAGGTAACCGCCATCACAGTTGACCAGCAAACCGCCGCTCTTGCTCAACAAACTGGAAGAAGGCTTAAAAGAGTTCTCGGAGTCTATCCATTTGTTATTTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

292

Amino Acids

32.45

Weight (kDa)

4.28

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 119 - 281 2.8e-39 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
AccBSI CCGCTC 1 cut(s) 809
AciI CCGC 4 cut(s) 757, 780, 804, 807
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 399
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 7 cut(s) 31, 65, 316, 389, 414, 475, 533
AhdI GACNNNNNGTC 1 cut(s) 574
Alw21I GWGCWC 2 cut(s) 15, 603
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 2 cut(s) 34, 650
AoxI GGCC 4 cut(s) 483, 502, 654, 716
ApeKI GCWGC 1 cut(s) 348
ApoI RAATTY 1 cut(s) 245
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 14
AvaI CYCGRG 2 cut(s) 34, 650
AvaII GGWCC 1 cut(s) 133
BalI TGGCCA 1 cut(s) 485
BanII GRGCYC 2 cut(s) 15, 603
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 2 cut(s) 468, 570
Bbv12I GWGCWC 2 cut(s) 15, 603
BbvI GCAGC 1 cut(s) 360
BccI CCATC 4 cut(s) 221, 662, 721, 791
BclI TGATCA 2 cut(s) 19, 121
BfaI CTAG 1 cut(s) 558
BfmI CTRYAG 1 cut(s) 55
BisI GCNGC 3 cut(s) 349, 757, 807
BlsI GCNGC 3 cut(s) 350, 758, 808
Bme18I GGWCC 1 cut(s) 133
BmeRI GACNNNNNGTC 1 cut(s) 574
BmeT110I CYCGRG 2 cut(s) 34, 650
BmgT120I GGNCC 1 cut(s) 133
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 1 cut(s) 579
BmuI ACTGGG 1 cut(s) 409
BpiI GAAGAC 2 cut(s) 468, 570
BpuEI CTTGAG 2 cut(s) 483, 755
BsaWI WCCGGW 1 cut(s) 446
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 3 cut(s) 404, 712, 829
BseGI GGATG 1 cut(s) 673
BseLI CCNNNNNNNGG 1 cut(s) 316
BseNI ACTGG 3 cut(s) 404, 712, 829
BseXI GCAGC 1 cut(s) 360
BshFI GGCC 4 cut(s) 485, 504, 656, 718
BsiHKAI GWGCWC 2 cut(s) 15, 603
BsiHKCI CYCGRG 2 cut(s) 34, 650
BsiSI CCGG 1 cut(s) 447
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 686
BsnI GGCC 4 cut(s) 485, 504, 656, 718
BsoBI CYCGRG 2 cut(s) 34, 650
Bsp1286I GDGCHC 2 cut(s) 15, 603
Bsp143I GATC 3 cut(s) 19, 43, 121
BspACI CCGC 4 cut(s) 757, 780, 804, 807
BspANI GGCC 4 cut(s) 485, 504, 656, 718
BsrBI CCGCTC 1 cut(s) 809
BsrI ACTGG 3 cut(s) 404, 712, 829
BssMI GATC 3 cut(s) 19, 43, 121
BssSI CACGAG 1 cut(s) 137
Bst2BI CACGAG 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 56, 790
Bst6I CTCTTC 2 cut(s) 336, 444
BstC8I GCNNGC 1 cut(s) 549
BstEII GGTNACC 1 cut(s) 775
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 673
BstKTI GATC 3 cut(s) 22, 46, 124
BstMBI GATC 3 cut(s) 19, 43, 121
BstMWI GCNNNNNNNGC 4 cut(s) 389, 598, 641, 812
BstPI GGTNACC 1 cut(s) 775
BstSFI CTRYAG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 360
BstV2I GAAGAC 2 cut(s) 468, 570
BsuRI GGCC 4 cut(s) 485, 504, 656, 718
BtsCI GGATG 1 cut(s) 673
BtsI GCAGTG 2 cut(s) 208, 747
BtsIMutI CAGTG 3 cut(s) 208, 719, 747
Cac8I GCNNGC 1 cut(s) 549
CaiI CAGNNNCTG 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 133
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 5 cut(s) 178, 287, 542, 680, 700
DpnI GATC 3 cut(s) 21, 45, 123
DpnII GATC 3 cut(s) 19, 43, 121
DrdI GACNNNNNNGTC 1 cut(s) 680
DriI GACNNNNNGTC 1 cut(s) 574
DseDI GACNNNNNNGTC 1 cut(s) 680
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 2 cut(s) 336, 444
Eam1105I GACNNNNNGTC 1 cut(s) 574
EarI CTCTTC 2 cut(s) 336, 444
Ecl136II GAGCTC 2 cut(s) 13, 601
Eco147I AGGCCT 1 cut(s) 504
Eco24I GRGCYC 2 cut(s) 15, 603
Eco47I GGWCC 1 cut(s) 133
Eco53kI GAGCTC 2 cut(s) 13, 601
Eco57I CTGAAG 1 cut(s) 399
Eco88I CYCGRG 2 cut(s) 34, 650
Eco91I GGTNACC 1 cut(s) 775
EcoICRI GAGCTC 2 cut(s) 13, 601
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO65I GGTNACC 1 cut(s) 775
EcoRI GAATTC 1 cut(s) 245
EcoT38I GRGCYC 2 cut(s) 15, 603
FaeI CATG 5 cut(s) 181, 290, 545, 683, 703
FaiI YATR 9 cut(s) 51, 179, 185, 260, 288, 419, 543, 681, 701
FaqI GGGAC 1 cut(s) 686
FatI CATG 5 cut(s) 177, 286, 541, 679, 699
FbaI TGATCA 2 cut(s) 19, 121
Fnu4HI GCNGC 3 cut(s) 349, 757, 807
FokI GGATG 1 cut(s) 680
FriOI GRGCYC 2 cut(s) 15, 603
Fsp4HI GCNGC 3 cut(s) 349, 757, 807
FspBI CTAG 1 cut(s) 558
GluI GCNGC 3 cut(s) 349, 757, 807
HaeIII GGCC 4 cut(s) 485, 504, 656, 718
HapII CCGG 1 cut(s) 447
Hin1II CATG 5 cut(s) 181, 290, 545, 683, 703
HincII GTYRAC 2 cut(s) 733, 793
HindII GTYRAC 2 cut(s) 733, 793
HinfI GANTC 7 cut(s) 65, 71, 167, 188, 322, 646, 852
HpaII CCGG 1 cut(s) 447
HphI GGTGA 1 cut(s) 14
Hpy166II GTNNAC 2 cut(s) 733, 793
Hpy188I TCNGA 6 cut(s) 70, 91, 133, 327, 588, 851
Hpy188III TCNNGA 3 cut(s) 224, 558, 650
Hpy8I GTNNAC 2 cut(s) 733, 793
HpyAV CCTTC 6 cut(s) 88, 293, 310, 515, 729, 825
HpyCH4III ACNGT 2 cut(s) 56, 790
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 1 cut(s) 740
HpyF10VI GCNNNNNNNGC 4 cut(s) 389, 598, 641, 812
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 5 cut(s) 181, 290, 545, 683, 703
Ksp22I TGATCA 2 cut(s) 19, 121
Kzo9I GATC 3 cut(s) 19, 43, 121
LmnI GCTCC 3 cut(s) 162, 374, 597
LpnPI CCDG 9 cut(s) 288, 323, 357, 385, 460, 561, 725, 809, 810
Lsp1109I GCAGC 1 cut(s) 360
LweI GCATC 1 cut(s) 579
MaeI CTAG 1 cut(s) 558
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 1 cut(s) 775
MalI GATC 3 cut(s) 21, 45, 123
MbiI CCGCTC 1 cut(s) 809
MboI GATC 3 cut(s) 19, 43, 121
MboII GAAGA 8 cut(s) 66, 278, 353, 431, 473, 484, 570, 840
MhlI GDGCHC 2 cut(s) 15, 603
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 3 cut(s) 245, 304, 479
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 2 cut(s) 182, 861
MnlI CCTC 5 cut(s) 155, 646, 658, 697, 738
Mox20I TGGCCA 1 cut(s) 485
MscI TGGCCA 1 cut(s) 485
MseI TTAA 4 cut(s) 353, 522, 615, 837
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 2 cut(s) 551, 759
MspI CCGG 1 cut(s) 447
MwoI GCNNNNNNNGC 4 cut(s) 389, 598, 641, 812
NdeII GATC 3 cut(s) 19, 43, 121
NlaIII CATG 5 cut(s) 181, 290, 545, 683, 703
PaeR7I CTCGAG 1 cut(s) 650
PceI AGGCCT 1 cut(s) 504
PfeI GAWTC 5 cut(s) 65, 71, 167, 322, 646
PkrI GCNGC 3 cut(s) 350, 758, 808
PleI GAGTC 2 cut(s) 182, 860
PpsI GAGTC 2 cut(s) 182, 860
Psp124BI GAGCTC 2 cut(s) 15, 603
PspEI GGTNACC 1 cut(s) 775
PspPI GGNCC 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 551
SacI GAGCTC 2 cut(s) 15, 603
SaqAI TTAA 4 cut(s) 353, 522, 615, 837
SatI GCNGC 3 cut(s) 349, 757, 807
Sau3AI GATC 3 cut(s) 19, 43, 121
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 2 cut(s) 182, 861
SduI GDGCHC 2 cut(s) 15, 603
SfaNI GCATC 1 cut(s) 579
SfcI CTRYAG 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 650
SinI GGWCC 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 650
SmlI CTYRAG 3 cut(s) 498, 650, 770
SmoI CTYRAG 3 cut(s) 498, 650, 770
Sse9I AATT 3 cut(s) 245, 304, 479
SseBI AGGCCT 1 cut(s) 504
SsiI CCGC 4 cut(s) 757, 780, 804, 807
SspMI CTAG 1 cut(s) 558
SstI GAGCTC 2 cut(s) 15, 603
StuI AGGCCT 1 cut(s) 504
TaaI ACNGT 2 cut(s) 56, 790
TaiI ACGT 1 cut(s) 491
TaqI TCGA 2 cut(s) 293, 651
TaqII GACCGA 1 cut(s) 209
TasI AATT 3 cut(s) 245, 304, 479
TauI GCSGC 2 cut(s) 759, 809
TfiI GAWTC 5 cut(s) 65, 71, 167, 322, 646
Tru1I TTAA 4 cut(s) 353, 522, 615, 837
Tru9I TTAA 4 cut(s) 353, 522, 615, 837
TscAI CASTG 3 cut(s) 208, 719, 747
TseI GCWGC 1 cut(s) 348
TspDTI ATGAA 5 cut(s) 474, 497, 570, 668, 711
TspRI CASTG 3 cut(s) 208, 719, 747
VpaK11BI GGWCC 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 1 cut(s) 245
XbaI TCTAGA 1 cut(s) 557
XhoI CTCGAG 1 cut(s) 650
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.