Rroxscaffold_2G00125180

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014643
Physical Location & Seq
Forward (+)
59777704 .. 59779020
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00125180.1

Sequence Viewer

Length: 1317 bp
ATGGACATAGACTCGTCTATCAGCAGTGGAAGTGATGTAATCGGTCTTGATGGTGTTGTTGATAGTGAATTCGCTTCTTACATAAGTGAAGAAGCACCAGAAAATGTCCATGTTTCGACAGAAGGCAATTCCCTTTTGAAGGAGATTCAGATAGATTTGCTGGAAGAGAGCAGCTTGACTGAACTTTTATTCGCAGGAGCTGAAGCTGTTGAAGCACAGAACTGGGCTTTTGCTTCAAATATAATCTCAAAGCTCAACACTCTTCTATCCGGTAGCGAAAATGGAGACAACTCGTTCAACAAATTGGCCACGTTCTTCACTCAAGGCCTTCATTACAAGACCATTAACTCTATTGAAGTTCCCCATGAGCCAGCTGTTTCTAGAAACACAGACACAATGTCTTCATTGCAGATGCTCCAAGAGCTCTCTCCTTTTGTTAAGTTTGCGCATTTCACAGCCAACCAAGCGATTCTCGAGGCCACACAAGAGGATGAGGACATTCATGTCATTGACTTTGACATCATGGAGGGTAGCCAGTGGCCTTCATTGATGGTTGACCTTGCAATGAGGAAAGGTCGCCGCTGTGCTTCTCTCAAGGTAACAGCCATCACAGTTGACCAGCAAAGCGCCGCTCTTGCTCAACAAACTGGAAGAAGGCTTAAAGGGTTCTCAGAGTCTATCAATTTGTTATTTATTTTTGATCAGATGATTATGGCCACAGAAGAAGATTTTGCTAAAATCGAAGTTGTTACTGATGCAGTAGTAGCCAATTGCATGATTCACCAGCTTCACATGCCTAACAGAAGTATCTCATTAGTGAAGGCCTTCTTGGGTGGAATGAGCAAATTATCACCTAAACTCATTGTCCTAGTACAAGAAGAATTGTTCAACTTTTCCAAAATGCCATCAATGGTCTTTGCAGAGTTCTTCTCGGAGGCTCTCCATCACTACAATTCTCTTTTCGACTCTGTTGTAACTAGTTTTAGTGGTGAGTATAAAATGGGGTTAAGACAGATAGAGAAAGAGGTTTTGGGGATCAAGATTTTAGAAAGTGTCAGGCAGTTCCCATGTGAGAAGGAGGATAGAATGTTGTGGGAAGATTGTTTTGCTTCGTTGAAGGGCTTCAAGTCTATCCCTTTAAGTTCTTGTAATGTTTCTCAAGCAAAATACTTGGTGAGTCTTTTCAATAGGGGATATTGGGTGCAGCATGAGAAGGGCAGGTTAGCTTTGTGTTGGAAATCAAGACCTTTGACAACAGCTTCCATTTGGGTGCCCTTAACTTACAAAATCAAGGAAAACTATGTCAACCCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

438

Amino Acids

49.09

Weight (kDa)

5.0

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 60 - 423 5.7e-79 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 503
Acc16I TGCGCA 1 cut(s) 447
Acc36I ACCTGC 1 cut(s) 1209
AccB1I GGYRCC 1 cut(s) 1270
AccBSI CCGCTC 1 cut(s) 632
AciI CCGC 2 cut(s) 580, 630
AclWI GGATC 1 cut(s) 1043
AcoI YGGCCR 2 cut(s) 306, 714
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 222
AfaI GTAC 1 cut(s) 873
AfiI CCNNNNNNNGG 1 cut(s) 139
AgsI TTSAA 9 cut(s) 139, 212, 237, 298, 356, 889, 1117, 1126, 1186
AhdI GACNNNNNGTC 1 cut(s) 397
AhlI ACTAGT 1 cut(s) 977
AjuI GAANNNNNNNTTGG 4 cut(s) 812, 844, 1013, 1045
AluBI AGCT 9 cut(s) 174, 200, 206, 253, 374, 424, 787, 1226, 1259
AluI AGCT 9 cut(s) 174, 200, 206, 253, 374, 424, 787, 1226, 1259
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 1 cut(s) 1043
AlwNI CAGNNNCTG 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 6 cut(s) 306, 325, 477, 539, 714, 822
ApeKI GCWGC 2 cut(s) 171, 1204
ApoI RAATTY 1 cut(s) 68
Asp700I GAANNNNTTC 2 cut(s) 824, 1121
AspLEI GCGC 2 cut(s) 448, 629
AsuHPI GGTGA 4 cut(s) 773, 843, 1001, 1186
AvaI CYCGRG 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 1275
BaeI ACNNNNGTAYC 2 cut(s) 790, 823
BalI TGGCCA 2 cut(s) 308, 716
BanI GGYRCC 1 cut(s) 1270
BanII GRGCYC 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 2 cut(s) 183, 1216
BccI CCATC 5 cut(s) 44, 544, 614, 913, 951
BclI TGATCA 1 cut(s) 700
BcoDI GTCTC 1 cut(s) 279
BcuI ACTAGT 1 cut(s) 977
BfaI CTAG 3 cut(s) 381, 869, 978
BfoI RGCGCY 1 cut(s) 630
BfuAI ACCTGC 1 cut(s) 1209
BisI GCNGC 4 cut(s) 172, 580, 630, 1205
BlsI GCNGC 4 cut(s) 173, 581, 631, 1206
BmeRI GACNNNNNGTC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 1272
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 2 cut(s) 402, 745
BmuI ACTGGG 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 393
BplI GAGNNNNNCTC 2 cut(s) 914, 946
BpuEI CTTGAG 3 cut(s) 306, 578, 1143
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse1I ACTGG 3 cut(s) 227, 535, 652
Bse3DI GCAATG 2 cut(s) 404, 570
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMI GCAATG 2 cut(s) 404, 570
BseMII CTCAG 1 cut(s) 684
BseNI ACTGG 3 cut(s) 227, 535, 652
BseSI GKGCMC 1 cut(s) 1275
BseXI GCAGC 2 cut(s) 183, 1216
BsgI GTGCAG 1 cut(s) 1223
BshFI GGCC 6 cut(s) 308, 327, 479, 541, 716, 824
BshNI GGYRCC 1 cut(s) 1270
BsiHKAI GWGCWC 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 1 cut(s) 270
BslI CCNNNNNNNGG 1 cut(s) 139
BsmAI GTCTC 1 cut(s) 279
BsnI GGCC 6 cut(s) 308, 327, 479, 541, 716, 824
BsoBI CYCGRG 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 426, 1275
Bsp143I GATC 2 cut(s) 700, 1035
BspACI CCGC 2 cut(s) 580, 630
BspANI GGCC 6 cut(s) 308, 327, 479, 541, 716, 824
BspCNI CTCAG 1 cut(s) 683
BspLI GGNNCC 1 cut(s) 1272
BspMI ACCTGC 1 cut(s) 1209
BspPI GGATC 1 cut(s) 1043
BspT107I GGYRCC 1 cut(s) 1270
BsrBI CCGCTC 1 cut(s) 632
BsrDI GCAATG 2 cut(s) 404, 570
BsrI ACTGG 3 cut(s) 227, 535, 652
BssMI GATC 2 cut(s) 700, 1035
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 2 cut(s) 159, 267
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 670
BstENI CCTNNNNNAGG 1 cut(s) 137
BstF5I GGATG 1 cut(s) 496
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 2 cut(s) 448, 629
BstKTI GATC 2 cut(s) 703, 1038
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 2 cut(s) 700, 1035
BstMWI GCNNNNNNNGC 6 cut(s) 212, 421, 464, 635, 764, 793
BstNSI RCATGY 1 cut(s) 796
BstSLI GKGCMC 1 cut(s) 1275
BstV1I GCAGC 2 cut(s) 183, 1216
BstV2I GAAGAC 1 cut(s) 393
BsuRI GGCC 6 cut(s) 308, 327, 479, 541, 716, 824
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 2 cut(s) 31, 542
BveI ACCTGC 1 cut(s) 1209
Cac8I GCNNGC 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 200
CfoI GCGC 2 cut(s) 448, 629
Csp6I GTAC 1 cut(s) 872
CviAII CATG 8 cut(s) 110, 365, 503, 523, 775, 793, 1068, 1208
CviQI GTAC 1 cut(s) 872
DdeI CTNAG 1 cut(s) 670
DpnI GATC 2 cut(s) 702, 1037
DpnII GATC 2 cut(s) 700, 1035
DrdI GACNNNNNNGTC 1 cut(s) 503
DriI GACNNNNNGTC 1 cut(s) 397
DseDI GACNNNNNNGTC 1 cut(s) 503
EaeI YGGCCR 2 cut(s) 306, 714
Eam1104I CTCTTC 2 cut(s) 159, 267
Eam1105I GACNNNNNGTC 1 cut(s) 397
EarI CTCTTC 2 cut(s) 159, 267
Ecl136II GAGCTC 1 cut(s) 424
Eco147I AGGCCT 2 cut(s) 327, 824
Eco24I GRGCYC 1 cut(s) 426
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 473
EcoICRI GAGCTC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 68
EcoT38I GRGCYC 1 cut(s) 426
FaeI CATG 8 cut(s) 113, 368, 506, 526, 778, 796, 1071, 1211
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FatI CATG 8 cut(s) 109, 364, 502, 522, 774, 792, 1067, 1207
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 4 cut(s) 172, 580, 630, 1205
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 4 cut(s) 172, 580, 630, 1205
FspBI CTAG 3 cut(s) 381, 869, 978
FspI TGCGCA 1 cut(s) 447
GlaI GCGC 2 cut(s) 447, 628
GluI GCNGC 4 cut(s) 172, 580, 630, 1205
HaeII RGCGCY 1 cut(s) 630
HaeIII GGCC 6 cut(s) 308, 327, 479, 541, 716, 824
HapII CCGG 1 cut(s) 270
HhaI GCGC 2 cut(s) 448, 629
Hin1II CATG 8 cut(s) 113, 368, 506, 526, 778, 796, 1071, 1211
Hin6I GCGC 2 cut(s) 446, 627
HinP1I GCGC 2 cut(s) 446, 627
HincII GTYRAC 3 cut(s) 556, 616, 1306
HindII GTYRAC 3 cut(s) 556, 616, 1306
HinfI GANTC 7 cut(s) 11, 145, 469, 674, 778, 965, 1177
HpaII CCGG 1 cut(s) 270
HphI GGTGA 4 cut(s) 773, 843, 1001, 1186
Hpy166II GTNNAC 3 cut(s) 556, 616, 1306
Hpy188I TCNGA 4 cut(s) 150, 673, 705, 934
Hpy188III TCNNGA 5 cut(s) 47, 381, 473, 1039, 1242
Hpy8I GTNNAC 3 cut(s) 556, 616, 1306
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 6 cut(s) 409, 563, 758, 774, 920, 1204
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 421, 464, 635, 764, 793
HpyF3I CTNAG 1 cut(s) 670
HpySE526I ACGT 1 cut(s) 311
Hsp92II CATG 8 cut(s) 113, 368, 506, 526, 778, 796, 1071, 1211
HspAI GCGC 2 cut(s) 446, 627
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 2 cut(s) 700, 1035
LmnI GCTCC 2 cut(s) 197, 420
Lsp1109I GCAGC 2 cut(s) 183, 1216
LweI GCATC 2 cut(s) 402, 745
MaeI CTAG 3 cut(s) 381, 869, 978
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 3 cut(s) 598, 748, 973
MalI GATC 2 cut(s) 702, 1037
MbiI CCGCTC 1 cut(s) 632
MboI GATC 2 cut(s) 700, 1035
MfeI CAATTG 1 cut(s) 769
MhlI GDGCHC 2 cut(s) 426, 1275
MlsI TGGCCA 2 cut(s) 308, 716
MluCI AATT 8 cut(s) 68, 127, 302, 682, 769, 845, 881, 952
MluNI TGGCCA 2 cut(s) 308, 716
MlyI GAGTC 4 cut(s) 5, 683, 959, 1186
MmeI TCCRAC 1 cut(s) 1214
MnlI CCTC 8 cut(s) 469, 481, 487, 520, 561, 928, 1018, 1072
Mox20I TGGCCA 2 cut(s) 308, 716
MroXI GAANNNNTTC 2 cut(s) 824, 1121
MscI TGGCCA 2 cut(s) 308, 716
MseI TTAA 6 cut(s) 345, 438, 660, 1007, 1139, 1277
MslI CAYNNNNRTG 1 cut(s) 1268
Msp20I TGGCCA 2 cut(s) 308, 716
MspA1I CMGCKG 2 cut(s) 374, 582
MspI CCGG 1 cut(s) 270
MunI CAATTG 1 cut(s) 769
MwoI GCNNNNNNNGC 6 cut(s) 212, 421, 464, 635, 764, 793
NdeII GATC 2 cut(s) 700, 1035
NlaIII CATG 8 cut(s) 113, 368, 506, 526, 778, 796, 1071, 1211
NlaIV GGNNCC 1 cut(s) 1272
NsbI TGCGCA 1 cut(s) 447
NspI RCATGY 1 cut(s) 796
PaeR7I CTCGAG 1 cut(s) 473
PceI AGGCCT 2 cut(s) 327, 824
PdmI GAANNNNTTC 2 cut(s) 824, 1121
PfeI GAWTC 3 cut(s) 145, 469, 778
PkrI GCNGC 4 cut(s) 173, 581, 631, 1206
PleI GAGTC 4 cut(s) 5, 682, 959, 1185
PpsI GAGTC 4 cut(s) 5, 682, 959, 1185
Psp124BI GAGCTC 1 cut(s) 426
PspN4I GGNNCC 1 cut(s) 1272
PstNI CAGNNNCTG 1 cut(s) 200
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 1 cut(s) 873
RsaNI GTAC 1 cut(s) 872
RseI CAYNNNNRTG 1 cut(s) 1268
SacI GAGCTC 1 cut(s) 426
SaqAI TTAA 6 cut(s) 345, 438, 660, 1007, 1139, 1277
SatI GCNGC 4 cut(s) 172, 580, 630, 1205
Sau3AI GATC 2 cut(s) 700, 1035
SchI GAGTC 4 cut(s) 5, 683, 959, 1186
SduI GDGCHC 2 cut(s) 426, 1275
SfaNI GCATC 2 cut(s) 402, 745
Sfr274I CTCGAG 1 cut(s) 473
SlaI CTCGAG 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 1268
SmlI CTYRAG 4 cut(s) 321, 473, 593, 1158
SmoI CTYRAG 4 cut(s) 321, 473, 593, 1158
SpeI ACTAGT 1 cut(s) 977
Sse9I AATT 8 cut(s) 68, 127, 302, 682, 769, 845, 881, 952
SseBI AGGCCT 2 cut(s) 327, 824
SsiI CCGC 2 cut(s) 580, 630
SspMI CTAG 3 cut(s) 381, 869, 978
SstI GAGCTC 1 cut(s) 426
StuI AGGCCT 2 cut(s) 327, 824
TaaI ACNGT 1 cut(s) 613
TaiI ACGT 1 cut(s) 314
TaqI TCGA 4 cut(s) 116, 474, 741, 963
TaqII GACCGA 1 cut(s) 32
TasI AATT 8 cut(s) 68, 127, 302, 682, 769, 845, 881, 952
TatI WGTACW 1 cut(s) 871
TauI GCSGC 2 cut(s) 582, 632
TfiI GAWTC 3 cut(s) 145, 469, 778
Tru1I TTAA 6 cut(s) 345, 438, 660, 1007, 1139, 1277
Tru9I TTAA 6 cut(s) 345, 438, 660, 1007, 1139, 1277
TscAI CASTG 2 cut(s) 31, 542
TseI GCWGC 2 cut(s) 171, 1204
TspDTI ATGAA 4 cut(s) 320, 393, 491, 534
TspRI CASTG 2 cut(s) 31, 542
XagI CCTNNNNNAGG 1 cut(s) 137
XapI RAATTY 1 cut(s) 68
XbaI TCTAGA 1 cut(s) 380
XceI RCATGY 1 cut(s) 796
XhoI CTCGAG 1 cut(s) 473
XmnI GAANNNNTTC 2 cut(s) 824, 1121
XspI CTAG 3 cut(s) 381, 869, 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.