Rw2G021940

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014643
Physical Location & Seq
Reverse (-)
30602214 .. 30605758
3545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G021940.1

Sequence Viewer

Length: 1224 bp
ATGGAAGTTGAGCTCATTGATCACCCATTCAACCCGAGCTTCGATCTCTATGACTGTAGCTTTGAATCTGATTCTTCCTTCCAAACATCAGATTTATTTACTCCTTGTACCACCAAAGTTGATCAAAACTCGGACCACGAGTGCCTCAACAGATTGCTCCAAATAGAATCACAGCTCATGGACATAGACTCGTCTATCAGCAGTGGAAGTGATGTAATCGGTCTTGATGGTGTTGTTGATAGTGAATTCGCTTCTTACATAAGTGAAGAAGCACCAGAAAATGTCCGTGTTTCGACAGAAGGCAATTCCCTTTTGAAGGAGATTCAGATAGATTTGCTGGAAGAGAGCAGCTTAACTGAACTTCTATTCGCAGGAGCTGAAGCGGTTGAATCACAGAACTGGGTTTTTGCTTCAAATATAATCGCAAAGCTCAACACTCTTCTATCCGGTAGCGAAAATGGAGACAACTCGTTCAACAAATTGGCCACGTTCTTCACTAAAGGCCTTCATTACAAGACCATTAACTCTATTGAAGTTCCCCATGAGCCAGCTGTTTCTAGAAACACAGAAACAATGTCTTCATTTCAGATGCTCCAAGTGCTCTCTCCTTTTGTTAAGTTTGCGCATTTCACAGCCAACCAAGCGATTCTCGAGGCCACACAAGAGGATGAGGACATTCATGTCATTGACTTTGACATCATGGAGGGTAGCCAGTGGCCTTCATTGATGGTTGATCTTGCAAGGAGGAAAGGTCGCCGCTGTGCTTCTCTCAAGGTAACCGCCATCACAGTTGACCAGCAAAGCACCGCTCTTGCTCAACAAACTGGAAGAAGGCTTAAAGAGTTCTCAGAGTCTATCAATTTGTTATTTATTTTTGATCAGATGATGATGGCCACAGAAGAAGATTTTGCTAAAATCGAAGTTGTTGCTGATGCAGTAGTAGCCAATTGCATGATTCACCAGCTTCACATGCCTAGCAGAAGTATCTCATTAATGAAGGCCTTCTTGGGTGGCATGAGCAAATTATCACCTAAACTCATTGTCTTAGTACAAGAAGAGTTGTTCAACTTTTCCAAAAAGCCATCAATGGTCTTTGCAGAGTTCTTCTCGGAGGCTCTCCATCACTACAATTCGCTTTGCGACTTTCTTGTAACTAGTTTTACACCTTATATGATGCCAATTTCGACAGTGGTCCACCGCGGAGCAGAGAGAACGCAGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

407

Amino Acids

45.49

Weight (kDa)

4.6

Isoelectric Point (pI)

54.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 119 - 387 3.8e-55 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
Acc16I TGCGCA 1 cut(s) 624
AccBSI CCGCTC 1 cut(s) 809
AccII CGCG 1 cut(s) 1200
AciI CCGC 6 cut(s) 383, 757, 780, 807, 1198, 1200
AcoI YGGCCR 2 cut(s) 483, 891
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 2 cut(s) 109, 1050
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 8 cut(s) 31, 65, 316, 389, 414, 475, 533, 1066
AhlI ACTAGT 1 cut(s) 1154
AjuI GAANNNNNNNTTGG 2 cut(s) 989, 1021
AluBI AGCT 9 cut(s) 13, 39, 60, 175, 351, 377, 430, 551, 964
AluI AGCT 9 cut(s) 13, 39, 60, 175, 351, 377, 430, 551, 964
Alw21I GWGCWC 2 cut(s) 15, 603
Alw26I GTCTC 1 cut(s) 456
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 2 cut(s) 34, 650
AoxI GGCC 6 cut(s) 483, 502, 654, 716, 891, 999
ApeKI GCWGC 1 cut(s) 348
ApoI RAATTY 1 cut(s) 245
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AseI ATTAAT 1 cut(s) 992
Asp700I GAANNNNTTC 1 cut(s) 1001
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 2 cut(s) 133, 1192
AsuHPI GGTGA 3 cut(s) 14, 950, 1020
AvaI CYCGRG 2 cut(s) 34, 650
AvaII GGWCC 2 cut(s) 133, 1192
BalI TGGCCA 2 cut(s) 485, 893
BanII GRGCYC 1 cut(s) 15
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 570
Bbv12I GWGCWC 2 cut(s) 15, 603
BbvI GCAGC 1 cut(s) 360
BccI CCATC 6 cut(s) 221, 721, 791, 883, 1090, 1128
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BclI TGATCA 3 cut(s) 19, 121, 877
BcoDI GTCTC 1 cut(s) 456
BcuI ACTAGT 1 cut(s) 1154
BfaI CTAG 3 cut(s) 558, 975, 1155
BfmI CTRYAG 1 cut(s) 55
BisI GCNGC 2 cut(s) 349, 757
BlsI GCNGC 2 cut(s) 350, 758
Bme18I GGWCC 2 cut(s) 133, 1192
BmeT110I CYCGRG 2 cut(s) 34, 650
BmgT120I GGNCC 2 cut(s) 133, 1192
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 3 cut(s) 579, 922, 1164
BmuI ACTGGG 1 cut(s) 409
BoxI GACNNNNGTC 1 cut(s) 1190
BpiI GAAGAC 1 cut(s) 570
BplI GAGNNNNNCTC 2 cut(s) 1091, 1123
BpuEI CTTGAG 1 cut(s) 755
BsaJI CCNNGG 1 cut(s) 1198
BsaWI WCCGGW 1 cut(s) 446
BsaXI ACNNNNNCTCC 2 cut(s) 736, 766
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 3 cut(s) 404, 712, 829
BseDI CCNNGG 1 cut(s) 1198
BseGI GGATG 1 cut(s) 673
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 861
BseNI ACTGG 3 cut(s) 404, 712, 829
BseXI GCAGC 1 cut(s) 360
Bsh1236I CGCG 1 cut(s) 1200
BshFI GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
BsiHKAI GWGCWC 2 cut(s) 15, 603
BsiHKCI CYCGRG 2 cut(s) 34, 650
BsiSI CCGG 1 cut(s) 447
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 456
BsnI GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
BsoBI CYCGRG 2 cut(s) 34, 650
Bsp1286I GDGCHC 2 cut(s) 15, 603
Bsp143I GATC 5 cut(s) 19, 43, 121, 733, 877
BspACI CCGC 6 cut(s) 383, 757, 780, 807, 1198, 1200
BspANI GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
BspCNI CTCAG 1 cut(s) 860
BspFNI CGCG 1 cut(s) 1200
BsrBI CCGCTC 1 cut(s) 809
BsrI ACTGG 3 cut(s) 404, 712, 829
BssECI CCNNGG 1 cut(s) 1198
BssMI GATC 5 cut(s) 19, 43, 121, 733, 877
BssSI CACGAG 1 cut(s) 137
Bst2BI CACGAG 1 cut(s) 137
Bst4CI ACNGT 3 cut(s) 56, 790, 1189
Bst6I CTCTTC 3 cut(s) 336, 444, 1050
BstC8I GCNNGC 1 cut(s) 549
BstDEI CTNAG 2 cut(s) 847, 1045
BstDSI CCRYGG 1 cut(s) 1198
BstEII GGTNACC 1 cut(s) 775
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 673
BstFNI CGCG 1 cut(s) 1200
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 5 cut(s) 22, 46, 124, 736, 880
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 5 cut(s) 19, 43, 121, 733, 877
BstMWI GCNNNNNNNGC 4 cut(s) 598, 641, 941, 970
BstNSI RCATGY 1 cut(s) 973
BstPAI GACNNNNGTC 1 cut(s) 1190
BstPI GGTNACC 1 cut(s) 775
BstSFI CTRYAG 1 cut(s) 55
BstUI CGCG 1 cut(s) 1200
BstV1I GCAGC 1 cut(s) 360
BstV2I GAAGAC 1 cut(s) 570
BsuRI GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
BtgI CCRYGG 1 cut(s) 1198
BtsCI GGATG 1 cut(s) 673
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 3 cut(s) 208, 719, 1194
Cac8I GCNNGC 1 cut(s) 549
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 1 cut(s) 625
Cfr13I GGNCC 2 cut(s) 133, 1192
Cfr42I CCGCGG 1 cut(s) 1201
Csp6I GTAC 2 cut(s) 108, 1049
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 7 cut(s) 178, 542, 680, 700, 952, 970, 1015
CviQI GTAC 2 cut(s) 108, 1049
DdeI CTNAG 2 cut(s) 847, 1045
DpnI GATC 5 cut(s) 21, 45, 123, 735, 879
DpnII GATC 5 cut(s) 19, 43, 121, 733, 877
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
EaeI YGGCCR 2 cut(s) 483, 891
Eam1104I CTCTTC 3 cut(s) 336, 444, 1050
EarI CTCTTC 3 cut(s) 336, 444, 1050
Ecl136II GAGCTC 1 cut(s) 13
Eco147I AGGCCT 2 cut(s) 504, 1001
Eco24I GRGCYC 1 cut(s) 15
Eco47I GGWCC 2 cut(s) 133, 1192
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 399
Eco88I CYCGRG 2 cut(s) 34, 650
Eco91I GGTNACC 1 cut(s) 775
EcoICRI GAGCTC 1 cut(s) 13
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO65I GGTNACC 1 cut(s) 775
EcoRI GAATTC 1 cut(s) 245
EcoT38I GRGCYC 1 cut(s) 15
FaeI CATG 7 cut(s) 181, 545, 683, 703, 955, 973, 1018
FalI AAGNNNNNCTT 2 cut(s) 989, 1021
FatI CATG 7 cut(s) 177, 541, 679, 699, 951, 969, 1014
FbaI TGATCA 3 cut(s) 19, 121, 877
Fnu4HI GCNGC 2 cut(s) 349, 757
FokI GGATG 1 cut(s) 680
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 2 cut(s) 349, 757
FspBI CTAG 3 cut(s) 558, 975, 1155
FspI TGCGCA 1 cut(s) 624
GlaI GCGC 1 cut(s) 624
GluI GCNGC 2 cut(s) 349, 757
HaeIII GGCC 6 cut(s) 485, 504, 656, 718, 893, 1001
HapII CCGG 1 cut(s) 447
HhaI GCGC 1 cut(s) 625
Hin1II CATG 7 cut(s) 181, 545, 683, 703, 955, 973, 1018
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HinfI GANTC 9 cut(s) 65, 71, 167, 188, 322, 389, 646, 851, 955
HpaII CCGG 1 cut(s) 447
HphI GGTGA 3 cut(s) 14, 950, 1020
Hpy166II GTNNAC 2 cut(s) 793, 1195
Hpy188I TCNGA 8 cut(s) 70, 91, 133, 327, 588, 850, 882, 1111
Hpy188III TCNNGA 3 cut(s) 224, 558, 650
Hpy8I GTNNAC 2 cut(s) 793, 1195
HpyAV CCTTC 8 cut(s) 88, 293, 310, 515, 729, 825, 991, 1012
HpyCH4III ACNGT 3 cut(s) 56, 790, 1189
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 4 cut(s) 740, 935, 951, 1097
HpyF10VI GCNNNNNNNGC 4 cut(s) 598, 641, 941, 970
HpyF3I CTNAG 2 cut(s) 847, 1045
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 7 cut(s) 181, 545, 683, 703, 955, 973, 1018
HspAI GCGC 1 cut(s) 623
Ksp22I TGATCA 3 cut(s) 19, 121, 877
KspI CCGCGG 1 cut(s) 1201
Kzo9I GATC 5 cut(s) 19, 43, 121, 733, 877
LmnI GCTCC 4 cut(s) 162, 374, 597, 1202
Lsp1109I GCAGC 1 cut(s) 360
LweI GCATC 3 cut(s) 579, 922, 1164
MaeI CTAG 3 cut(s) 558, 975, 1155
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 775, 1150
MalI GATC 5 cut(s) 21, 45, 123, 735, 879
MbiI CCGCTC 1 cut(s) 809
MboI GATC 5 cut(s) 19, 43, 121, 733, 877
MfeI CAATTG 1 cut(s) 946
MhlI GDGCHC 2 cut(s) 15, 603
MlsI TGGCCA 2 cut(s) 485, 893
MluCI AATT 8 cut(s) 245, 304, 479, 859, 946, 1022, 1129, 1179
MluNI TGGCCA 2 cut(s) 485, 893
MlyI GAGTC 2 cut(s) 182, 860
MnlI CCTC 7 cut(s) 155, 646, 658, 664, 697, 738, 1105
Mox20I TGGCCA 2 cut(s) 485, 893
MroXI GAANNNNTTC 1 cut(s) 1001
MscI TGGCCA 2 cut(s) 485, 893
MseI TTAA 5 cut(s) 353, 522, 615, 837, 992
Msp20I TGGCCA 2 cut(s) 485, 893
MspA1I CMGCKG 3 cut(s) 551, 759, 1200
MspI CCGG 1 cut(s) 447
MunI CAATTG 1 cut(s) 946
MvnI CGCG 1 cut(s) 1200
MwoI GCNNNNNNNGC 4 cut(s) 598, 641, 941, 970
NdeII GATC 5 cut(s) 19, 43, 121, 733, 877
NlaIII CATG 7 cut(s) 181, 545, 683, 703, 955, 973, 1018
NsbI TGCGCA 1 cut(s) 624
NspI RCATGY 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 650
PceI AGGCCT 2 cut(s) 504, 1001
PdmI GAANNNNTTC 1 cut(s) 1001
PfeI GAWTC 7 cut(s) 65, 71, 167, 322, 389, 646, 955
PkrI GCNGC 2 cut(s) 350, 758
PleI GAGTC 2 cut(s) 182, 859
PpsI GAGTC 2 cut(s) 182, 859
PshAI GACNNNNGTC 1 cut(s) 1190
PshBI ATTAAT 1 cut(s) 992
Psp124BI GAGCTC 1 cut(s) 15
PspEI GGTNACC 1 cut(s) 775
PspPI GGNCC 2 cut(s) 133, 1192
PstNI CAGNNNCTG 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 2 cut(s) 109, 1050
RsaNI GTAC 2 cut(s) 108, 1049
SacI GAGCTC 1 cut(s) 15
SacII CCGCGG 1 cut(s) 1201
SaqAI TTAA 5 cut(s) 353, 522, 615, 837, 992
SatI GCNGC 2 cut(s) 349, 757
Sau3AI GATC 5 cut(s) 19, 43, 121, 733, 877
Sau96I GGNCC 2 cut(s) 133, 1192
SchI GAGTC 2 cut(s) 182, 860
SduI GDGCHC 2 cut(s) 15, 603
SfaNI GCATC 3 cut(s) 579, 922, 1164
SfcI CTRYAG 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 650
Sfr303I CCGCGG 1 cut(s) 1201
SgrBI CCGCGG 1 cut(s) 1201
SinI GGWCC 2 cut(s) 133, 1192
SlaI CTCGAG 1 cut(s) 650
SmlI CTYRAG 2 cut(s) 650, 770
SmoI CTYRAG 2 cut(s) 650, 770
SpeI ACTAGT 1 cut(s) 1154
Sse9I AATT 8 cut(s) 245, 304, 479, 859, 946, 1022, 1129, 1179
SseBI AGGCCT 2 cut(s) 504, 1001
SsiI CCGC 6 cut(s) 383, 757, 780, 807, 1198, 1200
SspMI CTAG 3 cut(s) 558, 975, 1155
SstI GAGCTC 1 cut(s) 15
StuI AGGCCT 2 cut(s) 504, 1001
TaaI ACNGT 3 cut(s) 56, 790, 1189
TaiI ACGT 1 cut(s) 491
TaqI TCGA 5 cut(s) 42, 293, 651, 918, 1184
TaqII GACCGA 1 cut(s) 209
TasI AATT 8 cut(s) 245, 304, 479, 859, 946, 1022, 1129, 1179
TatI WGTACW 1 cut(s) 1048
TauI GCSGC 1 cut(s) 759
TfiI GAWTC 7 cut(s) 65, 71, 167, 322, 389, 646, 955
Tru1I TTAA 5 cut(s) 353, 522, 615, 837, 992
Tru9I TTAA 5 cut(s) 353, 522, 615, 837, 992
TscAI CASTG 3 cut(s) 208, 719, 1194
TseI GCWGC 1 cut(s) 348
TspDTI ATGAA 5 cut(s) 497, 570, 668, 711, 1010
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 3 cut(s) 208, 719, 1194
VpaK11BI GGWCC 2 cut(s) 133, 1192
VspI ATTAAT 1 cut(s) 992
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 1 cut(s) 245
XbaI TCTAGA 1 cut(s) 557
XceI RCATGY 1 cut(s) 973
XhoI CTCGAG 1 cut(s) 650
XmnI GAANNNNTTC 1 cut(s) 1001
XspI CTAG 3 cut(s) 558, 975, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.