RchiOBHm_Chr2g0118801

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014643
Physical Location & Seq
Reverse (-)
30986093 .. 30987163
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49155

Sequence Viewer

Length: 1071 bp
ATGGAAGTTGAGCTCATTGATCACCCATTCAACCCGAGCTTCGATCTCTATGACTGTAGCTTTGAATCTGATTCTTCCTTCCAAACATCAGATTTATTTACTCGTACCACCAATGTTGATCAAAACTCGGACCACGAGTGCCTCAACAGAATGCTCCAAATAGAATCACAGCTCATGGACATAGACTCGTCTATCAGCAGTGGAAGTGATGTAATCGGTCTTGATGGTGTTGTTGATAGTGAATTCGCTTCTTACATAAGTGAAGAAGCACCAGAAAATGTCCGTGTTTCGACAGAAGGCAATTCCCTTTTGAAGGAGATTCAGATAGATTTGCTGGAAGAGAGCAGCTTAACTGAACTTCTATTCGCAGGAGCTGAAGCGGTTGAATCACAGAACTGGGTTTTTGCTTCAAATATAATCGCAAAGCTCAACACTCTTCTATCCGGTAGCGAAAATGGAGACAACTCGTTCAACAAATTGGCCACGTTCTTCACTAAAGGCCTTCATTACAAGACCATTAACTCTATTGAAGTTCCCCATGAGCCAGCTGTTTCTAGAAACACAGAAACAATGTCTTCATTTCAGATGCTCCTAGAGCTCTCTCCTTTTGTTAAGTTTGCGCATTTCACAGCCAACCAAGCGATTCTCGAGGCCACACAAGAGGATGAGGACATTCATGTCATTGACTTTGACATCATGGAGGGTAGCCAGTGGCCTTCATTGATGGTTGATCTTGCAAGGAGGAAAGGTCGCCGCTGTGCTTCTCTCAAGGTAACCGCCATCACAGTTGACCAGCAAAGCACCGCTCTTGCTCAACAAACTGGAAGAAGTAGGGCTGCACACGAATTGGATTGGATCGGTTTTGACCCCAAACCATCTCTATGCCAAAGTGAGCGGTTTATGCATTTTGAACAACCGACCATCAGAAAAAATAAGCATAGTCCAATCCGATCCAATTCAATTAAAGATGGTTTGGTTCGGTCGGTTAGGCGGTTTAACCCTAATAATATTATTTATAAAAAAAAAATCATAGTAAAATTCAATCTCAATAATAAAAATTATGTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

356

Amino Acids

40.18

Weight (kDa)

4.98

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 118 - 275 5.1e-38 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
AasI GACNNNNNNGTC 1 cut(s) 677
Acc16I TGCGCA 1 cut(s) 621
AccBSI CCGCTC 2 cut(s) 806, 895
AciI CCGC 6 cut(s) 380, 754, 777, 804, 895, 991
AclWI GGATC 2 cut(s) 863, 945
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 2 cut(s) 242, 1037
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 1 cut(s) 313
AluBI AGCT 9 cut(s) 13, 39, 60, 172, 348, 374, 427, 548, 598
AluI AGCT 9 cut(s) 13, 39, 60, 172, 348, 374, 427, 548, 598
Alw21I GWGCWC 2 cut(s) 15, 600
Alw26I GTCTC 1 cut(s) 453
AlwI GGATC 2 cut(s) 863, 945
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 2 cut(s) 34, 647
AoxI GGCC 4 cut(s) 480, 499, 651, 713
ApeKI GCWGC 2 cut(s) 345, 836
ApoI RAATTY 2 cut(s) 242, 1037
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 14
AvaI CYCGRG 2 cut(s) 34, 647
AvaII GGWCC 1 cut(s) 130
BalI TGGCCA 1 cut(s) 482
BanII GRGCYC 2 cut(s) 15, 600
BauI CACGAG 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 2 cut(s) 15, 600
BbvI GCAGC 2 cut(s) 357, 823
BccI CCATC 6 cut(s) 218, 718, 788, 883, 929, 962
BclI TGATCA 2 cut(s) 19, 118
BcoDI GTCTC 1 cut(s) 453
BfaI CTAG 2 cut(s) 555, 593
BfmI CTRYAG 1 cut(s) 55
BisI GCNGC 3 cut(s) 346, 754, 837
BlsI GCNGC 3 cut(s) 347, 755, 838
Bme18I GGWCC 1 cut(s) 130
BmeT110I CYCGRG 2 cut(s) 34, 647
BmgT120I GGNCC 1 cut(s) 130
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 1 cut(s) 576
BmuI ACTGGG 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 752
BsaWI WCCGGW 1 cut(s) 443
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 3 cut(s) 401, 709, 826
BseGI GGATG 1 cut(s) 670
BseLI CCNNNNNNNGG 1 cut(s) 313
BseNI ACTGG 3 cut(s) 401, 709, 826
BseXI GCAGC 2 cut(s) 357, 823
BsgI GTGCAG 1 cut(s) 822
Bsh1285I CGRYCG 1 cut(s) 983
BshFI GGCC 4 cut(s) 482, 501, 653, 715
BsiEI CGRYCG 1 cut(s) 983
BsiHKAI GWGCWC 2 cut(s) 15, 600
BsiHKCI CYCGRG 2 cut(s) 34, 647
BsiSI CCGG 1 cut(s) 444
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 1 cut(s) 453
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 4 cut(s) 482, 501, 653, 715
BsoBI CYCGRG 2 cut(s) 34, 647
Bsp1286I GDGCHC 2 cut(s) 15, 600
Bsp143I GATC 6 cut(s) 19, 43, 118, 730, 855, 950
BspACI CCGC 6 cut(s) 380, 754, 777, 804, 895, 991
BspANI GGCC 4 cut(s) 482, 501, 653, 715
BspPI GGATC 2 cut(s) 863, 945
BsrBI CCGCTC 2 cut(s) 806, 895
BsrI ACTGG 3 cut(s) 401, 709, 826
BssMI GATC 6 cut(s) 19, 43, 118, 730, 855, 950
BssSI CACGAG 1 cut(s) 134
Bst2BI CACGAG 1 cut(s) 134
Bst4CI ACNGT 2 cut(s) 56, 787
Bst6I CTCTTC 2 cut(s) 333, 441
BstC8I GCNNGC 1 cut(s) 546
BstEII GGTNACC 1 cut(s) 772
BstENI CCTNNNNNAGG 1 cut(s) 311
BstF5I GGATG 1 cut(s) 670
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 6 cut(s) 22, 46, 121, 733, 858, 953
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 6 cut(s) 19, 43, 118, 730, 855, 950
BstMCI CGRYCG 1 cut(s) 983
BstMWI GCNNNNNNNGC 3 cut(s) 595, 638, 901
BstPI GGTNACC 1 cut(s) 772
BstSFI CTRYAG 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 357, 823
BstV2I GAAGAC 1 cut(s) 567
BsuRI GGCC 4 cut(s) 482, 501, 653, 715
BtsCI GGATG 1 cut(s) 670
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 2 cut(s) 205, 716
Cac8I GCNNGC 1 cut(s) 546
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 1 cut(s) 622
Cfr13I GGNCC 1 cut(s) 130
Csp6I GTAC 1 cut(s) 105
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 4 cut(s) 175, 539, 677, 697
CviQI GTAC 1 cut(s) 105
DpnI GATC 6 cut(s) 21, 45, 120, 732, 857, 952
DpnII GATC 6 cut(s) 19, 43, 118, 730, 855, 950
DrdI GACNNNNNNGTC 1 cut(s) 677
DseDI GACNNNNNNGTC 1 cut(s) 677
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 2 cut(s) 333, 441
EarI CTCTTC 2 cut(s) 333, 441
Ecl136II GAGCTC 2 cut(s) 13, 598
Eco147I AGGCCT 1 cut(s) 501
Eco24I GRGCYC 2 cut(s) 15, 600
Eco47I GGWCC 1 cut(s) 130
Eco53kI GAGCTC 2 cut(s) 13, 598
Eco57I CTGAAG 1 cut(s) 396
Eco88I CYCGRG 2 cut(s) 34, 647
Eco91I GGTNACC 1 cut(s) 772
EcoICRI GAGCTC 2 cut(s) 13, 598
EcoNI CCTNNNNNAGG 1 cut(s) 311
EcoO65I GGTNACC 1 cut(s) 772
EcoRI GAATTC 1 cut(s) 242
EcoT22I ATGCAT 1 cut(s) 906
EcoT38I GRGCYC 2 cut(s) 15, 600
FaeI CATG 4 cut(s) 178, 542, 680, 700
FatI CATG 4 cut(s) 174, 538, 676, 696
FbaI TGATCA 2 cut(s) 19, 118
Fnu4HI GCNGC 3 cut(s) 346, 754, 837
FokI GGATG 1 cut(s) 677
FriOI GRGCYC 2 cut(s) 15, 600
Fsp4HI GCNGC 3 cut(s) 346, 754, 837
FspBI CTAG 2 cut(s) 555, 593
FspI TGCGCA 1 cut(s) 621
GlaI GCGC 1 cut(s) 621
GluI GCNGC 3 cut(s) 346, 754, 837
HaeIII GGCC 4 cut(s) 482, 501, 653, 715
HapII CCGG 1 cut(s) 444
HhaI GCGC 1 cut(s) 622
Hin1II CATG 4 cut(s) 178, 542, 680, 700
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HinfI GANTC 7 cut(s) 65, 71, 164, 185, 319, 386, 643
HpaII CCGG 1 cut(s) 444
HphI GGTGA 1 cut(s) 14
Hpy166II GTNNAC 1 cut(s) 790
Hpy188I TCNGA 7 cut(s) 70, 91, 130, 324, 585, 926, 950
Hpy188III TCNNGA 3 cut(s) 221, 555, 647
Hpy8I GTNNAC 1 cut(s) 790
HpyAV CCTTC 5 cut(s) 88, 290, 307, 512, 726
HpyCH4III ACNGT 2 cut(s) 56, 787
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 3 cut(s) 737, 839, 904
HpyF10VI GCNNNNNNNGC 3 cut(s) 595, 638, 901
HpySE526I ACGT 1 cut(s) 485
Hsp92II CATG 4 cut(s) 178, 542, 680, 700
HspAI GCGC 1 cut(s) 620
Ksp22I TGATCA 2 cut(s) 19, 118
Kzo9I GATC 6 cut(s) 19, 43, 118, 730, 855, 950
LmnI GCTCC 3 cut(s) 159, 371, 594
LpnPI CCDG 9 cut(s) 285, 320, 354, 382, 457, 558, 722, 806, 807
Lsp1109I GCAGC 2 cut(s) 357, 823
LweI GCATC 1 cut(s) 576
MaeI CTAG 2 cut(s) 555, 593
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 1 cut(s) 772
MalI GATC 6 cut(s) 21, 45, 120, 732, 857, 952
MbiI CCGCTC 2 cut(s) 806, 895
MboI GATC 6 cut(s) 19, 43, 118, 730, 855, 950
MboII GAAGA 7 cut(s) 66, 275, 350, 428, 481, 567, 837
MhlI GDGCHC 2 cut(s) 15, 600
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 8 cut(s) 242, 301, 476, 845, 955, 960, 1037, 1057
MluNI TGGCCA 1 cut(s) 482
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 6 cut(s) 152, 643, 655, 661, 694, 735
Mox20I TGGCCA 1 cut(s) 482
Mph1103I ATGCAT 1 cut(s) 906
MscI TGGCCA 1 cut(s) 482
MseI TTAA 6 cut(s) 350, 519, 612, 963, 996, 1065
MslI CAYNNNNRTG 1 cut(s) 880
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 2 cut(s) 548, 756
MspI CCGG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 595, 638, 901
NdeII GATC 6 cut(s) 19, 43, 118, 730, 855, 950
NlaIII CATG 4 cut(s) 178, 542, 680, 700
NsbI TGCGCA 1 cut(s) 621
NsiI ATGCAT 1 cut(s) 906
PaeR7I CTCGAG 1 cut(s) 647
PceI AGGCCT 1 cut(s) 501
PctI GAATGC 1 cut(s) 156
PfeI GAWTC 6 cut(s) 65, 71, 164, 319, 386, 643
PkrI GCNGC 3 cut(s) 347, 755, 838
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PsiI TTATAA 1 cut(s) 1017
Psp124BI GAGCTC 2 cut(s) 15, 600
PspEI GGTNACC 1 cut(s) 772
PspPI GGNCC 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
RseI CAYNNNNRTG 1 cut(s) 880
SacI GAGCTC 2 cut(s) 15, 600
SaqAI TTAA 6 cut(s) 350, 519, 612, 963, 996, 1065
SatI GCNGC 3 cut(s) 346, 754, 837
Sau3AI GATC 6 cut(s) 19, 43, 118, 730, 855, 950
Sau96I GGNCC 1 cut(s) 130
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 2 cut(s) 15, 600
SfaNI GCATC 1 cut(s) 576
SfcI CTRYAG 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 647
SinI GGWCC 1 cut(s) 130
SlaI CTCGAG 1 cut(s) 647
SmiMI CAYNNNNRTG 1 cut(s) 880
SmlI CTYRAG 2 cut(s) 647, 767
SmoI CTYRAG 2 cut(s) 647, 767
Sse9I AATT 8 cut(s) 242, 301, 476, 845, 955, 960, 1037, 1057
SseBI AGGCCT 1 cut(s) 501
SsiI CCGC 6 cut(s) 380, 754, 777, 804, 895, 991
SspI AATATT 1 cut(s) 1009
SspMI CTAG 2 cut(s) 555, 593
SstI GAGCTC 2 cut(s) 15, 600
StuI AGGCCT 1 cut(s) 501
TaaI ACNGT 2 cut(s) 56, 787
TaiI ACGT 1 cut(s) 488
TaqI TCGA 3 cut(s) 42, 290, 648
TaqII GACCGA 2 cut(s) 206, 969
TasI AATT 8 cut(s) 242, 301, 476, 845, 955, 960, 1037, 1057
TauI GCSGC 1 cut(s) 756
TfiI GAWTC 6 cut(s) 65, 71, 164, 319, 386, 643
Tru1I TTAA 6 cut(s) 350, 519, 612, 963, 996, 1065
Tru9I TTAA 6 cut(s) 350, 519, 612, 963, 996, 1065
TscAI CASTG 2 cut(s) 205, 716
TseI GCWGC 2 cut(s) 345, 836
TspDTI ATGAA 4 cut(s) 494, 567, 665, 708
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 2 cut(s) 205, 716
VpaK11BI GGWCC 1 cut(s) 130
XagI CCTNNNNNAGG 1 cut(s) 311
XapI RAATTY 2 cut(s) 242, 1037
XbaI TCTAGA 1 cut(s) 554
XhoI CTCGAG 1 cut(s) 647
XspI CTAG 2 cut(s) 555, 593
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.