RLG00000018421

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014643
Physical Location & Seq
Reverse (-)
29555978 .. 29558225
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018421

Sequence Viewer

Length: 1434 bp
ATGGAAGTTGAGCTCATTGATCGCCCATTCAACCCCAGCTACGACCTCTATGACTGTAGCTTCGAATCTGATTCTTCCTTCCAAACATCAGATTTATTGACTCCTAGTACCACCAATGTTGATCAAAACTCGGTCTCGGACCACGAGTGCCTCAACAGATTGCTCCAAATAGAATCACAGCTCATGGACATAGACTCGTCTATCAGCAGTGGAAGTGATGTAATCTGTCTTGATGGTGTTGTTGATAGTGAATTCGCTTACATAAGTGAAGAAGCACCAGAAAATGTCCATGTTTCGACAGAAGGCAATTCCCTTTTGAAGGAGATTCAGATAGATTTGCTGGAAGAGAGCAGCTTAACTGAACTTTTATTCGCAGGAGCTGATGCTGTTGAAGCACAGAACTGGGTTTTTGCTTCAAATATAATCGCAAAGCTCAACACTCTTCTATCCGGTAGCGAAAATGGAGACAACTCGTTCAACAAATTGGCCACGTTCTTCACTCAAGGCCTTCATTACAAGACCATTAACTATATTGAAGTTCCCCATGAGCCAGCTGTTTCTAGAAACACAGACACAATGTCTTCATTTCAGATGCTCCAAGAGCTCTCTCCTTTTGTTAAGTTTGCGCATTTCACAGCCAACCAAGCGATTCTCGAGGCCACACAAGAGGATGAGGACATTCATGTCATTGACTTTGACATCATGGAGGGTAGCCAGTGGCCTTCATTGATGGGTGACCTTGCAATGAGGAAAGGTCGCCGCTGTGCTTCTCTCAAGGTAACTGCCATCACAGTCGACCAGAAAAGCACCGCTCTTGCTCAACAAACTGGAAGAAGGCTTAAAGAGTTCTCAGAGTCTATCAATTTGTTATTTATTTTTGATCAGATGATGATGGCCACAGAAGAAGATTTTGCTAAAATCGAAGTTGTTGCTGATGCAGTAGTAGCCAATTGCATGATTCACCAGCTTCACATGCCTAACAGAAGTATCTCATTAGTGAAGGCCTTCTTGGGTGGCATGAGCAAATTATCACCTAAACTCGTTGTCCTAGTACAAGAAGAGTTGTTCAACTTTTCCAAAATGCCATCAATGGTCTTTGCAGAGTTCTTCTCGGAGGCTCTCCATCACTACAATTCCCTTTGCGACTCTCTTGTAACTAGTTTTAGTGGTGAGTATAAAATGGGGTTAAGACAGATAGAGAAAGAGGTTTTGGGGATCAAGATTTTAGAAAGTGTCAGGCAGTTCCCATGTGAGAAGGAGGATAGAATGTTGTGGGAAGATTGTTTTGCTTCGTTGAAGGGCTTCAAGTCTATCCCTTTAAGTTCTTGTAATGTTTCTCAAGCAAAATACTTGGTGAGTCTTTTCAATAGGGGATACTGGGTGCAGCATGAGAAGGGCAGGTCTTTACTTGATGAGCTAACATTTTCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

478

Amino Acids

53.59

Weight (kDa)

4.6

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 120 - 469 5.8e-69 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 683
Acc16I TGCGCA 1 cut(s) 627
Acc36I ACCTGC 1 cut(s) 1389
AccBSI CCGCTC 1 cut(s) 812
AccI GTMKAC 1 cut(s) 795
AciI CCGC 2 cut(s) 760, 810
AclWI GGATC 1 cut(s) 1223
AcoI YGGCCR 2 cut(s) 486, 894
AcsI RAATTY 1 cut(s) 251
AfaI GTAC 2 cut(s) 109, 1053
AfiI CCNNNNNNNGG 1 cut(s) 319
AhdI GACNNNNNGTC 1 cut(s) 577
AhlI ACTAGT 1 cut(s) 1157
AjuI GAANNNNNNNTTGG 4 cut(s) 992, 1024, 1193, 1225
Alw21I GWGCWC 2 cut(s) 15, 606
Alw26I GTCTC 2 cut(s) 139, 459
AlwI GGATC 1 cut(s) 1223
AlwNI CAGNNNCTG 1 cut(s) 380
Ama87I CYCGRG 1 cut(s) 653
AoxI GGCC 6 cut(s) 486, 505, 657, 719, 894, 1002
ApeKI GCWGC 2 cut(s) 351, 1384
ApoI RAATTY 1 cut(s) 251
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 2 cut(s) 1004, 1301
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 5 cut(s) 746, 953, 1023, 1181, 1366
AsuII TTCGAA 1 cut(s) 63
AvaI CYCGRG 1 cut(s) 653
AvaII GGWCC 1 cut(s) 139
BaeI ACNNNNGTAYC 2 cut(s) 970, 1003
BalI TGGCCA 2 cut(s) 488, 896
BanII GRGCYC 2 cut(s) 15, 606
BauI CACGAG 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 573
Bbv12I GWGCWC 2 cut(s) 15, 606
BbvI GCAGC 2 cut(s) 363, 1396
BccI CCATC 6 cut(s) 227, 724, 794, 886, 1093, 1131
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciVI GTATCC 1 cut(s) 1367
BclI TGATCA 2 cut(s) 121, 880
BcoDI GTCTC 2 cut(s) 139, 459
BcuI ACTAGT 1 cut(s) 1157
BfaI CTAG 4 cut(s) 105, 561, 1049, 1158
BfmI CTRYAG 1 cut(s) 55
BfuAI ACCTGC 1 cut(s) 1389
BfuI GTATCC 1 cut(s) 1367
BisI GCNGC 3 cut(s) 352, 760, 1385
BlsI GCNGC 3 cut(s) 353, 761, 1386
Bme18I GGWCC 1 cut(s) 139
BmeRI GACNNNNNGTC 1 cut(s) 577
BmeT110I CYCGRG 1 cut(s) 653
BmgT120I GGNCC 1 cut(s) 139
BmrI ACTGGG 2 cut(s) 412, 1387
BmsI GCATC 3 cut(s) 373, 582, 925
BmuI ACTGGG 2 cut(s) 412, 1387
BpiI GAAGAC 1 cut(s) 573
BplI GAGNNNNNCTC 2 cut(s) 1094, 1126
Bpu14I TTCGAA 1 cut(s) 63
BpuEI CTTGAG 3 cut(s) 486, 758, 1323
BsaI GGTCTC 1 cut(s) 139
BsaWI WCCGGW 1 cut(s) 449
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse1I ACTGG 4 cut(s) 407, 715, 832, 1382
Bse3DI GCAATG 1 cut(s) 750
BseGI GGATG 1 cut(s) 676
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 1 cut(s) 864
BseNI ACTGG 4 cut(s) 407, 715, 832, 1382
BseXI GCAGC 2 cut(s) 363, 1396
BseYI CCCAGC 1 cut(s) 35
BsgI GTGCAG 1 cut(s) 1403
BshFI GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
BsiHKAI GWGCWC 2 cut(s) 15, 606
BsiHKCI CYCGRG 1 cut(s) 653
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 2 cut(s) 139, 459
BsnI GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 653
Bsp119I TTCGAA 1 cut(s) 63
Bsp1286I GDGCHC 2 cut(s) 15, 606
Bsp143I GATC 4 cut(s) 19, 121, 880, 1215
BspACI CCGC 2 cut(s) 760, 810
BspANI GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
BspCNI CTCAG 1 cut(s) 863
BspHI TCATGA 1 cut(s) 1430
BspMI ACCTGC 1 cut(s) 1389
BspPI GGATC 1 cut(s) 1223
BspT104I TTCGAA 1 cut(s) 63
BspTNI GGTCTC 1 cut(s) 139
BsrBI CCGCTC 1 cut(s) 812
BsrDI GCAATG 1 cut(s) 750
BsrI ACTGG 4 cut(s) 407, 715, 832, 1382
BssMI GATC 4 cut(s) 19, 121, 880, 1215
BssSI CACGAG 1 cut(s) 143
Bst2BI CACGAG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 56, 793
Bst6I CTCTTC 3 cut(s) 339, 447, 1053
BstBI TTCGAA 1 cut(s) 63
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 1 cut(s) 850
BstEII GGTNACC 1 cut(s) 734
BstENI CCTNNNNNAGG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 676
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 4 cut(s) 22, 124, 883, 1218
BstMAI GTCTC 2 cut(s) 139, 459
BstMBI GATC 4 cut(s) 19, 121, 880, 1215
BstMWI GCNNNNNNNGC 5 cut(s) 392, 601, 644, 944, 973
BstNSI RCATGY 1 cut(s) 976
BstPI GGTNACC 1 cut(s) 734
BstSFI CTRYAG 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 363, 1396
BstV2I GAAGAC 1 cut(s) 573
BsuI GTATCC 1 cut(s) 1367
BsuRI GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
BtsCI GGATG 1 cut(s) 676
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 214, 722
BveI ACCTGC 1 cut(s) 1389
Cac8I GCNNGC 1 cut(s) 552
CaiI CAGNNNCTG 1 cut(s) 380
CciI TCATGA 1 cut(s) 1430
CfoI GCGC 1 cut(s) 628
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 108, 1052
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviQI GTAC 2 cut(s) 108, 1052
DdeI CTNAG 1 cut(s) 850
DpnI GATC 4 cut(s) 21, 123, 882, 1217
DpnII GATC 4 cut(s) 19, 121, 880, 1215
DrdI GACNNNNNNGTC 1 cut(s) 683
DriI GACNNNNNGTC 1 cut(s) 577
DseDI GACNNNNNNGTC 1 cut(s) 683
EaeI YGGCCR 2 cut(s) 486, 894
Eam1104I CTCTTC 3 cut(s) 339, 447, 1053
Eam1105I GACNNNNNGTC 1 cut(s) 577
EarI CTCTTC 3 cut(s) 339, 447, 1053
Ecl136II GAGCTC 2 cut(s) 13, 604
Eco147I AGGCCT 2 cut(s) 507, 1004
Eco24I GRGCYC 2 cut(s) 15, 606
Eco31I GGTCTC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 139
Eco53kI GAGCTC 2 cut(s) 13, 604
Eco88I CYCGRG 1 cut(s) 653
Eco91I GGTNACC 1 cut(s) 734
EcoICRI GAGCTC 2 cut(s) 13, 604
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoO65I GGTNACC 1 cut(s) 734
EcoRI GAATTC 1 cut(s) 251
EcoT38I GRGCYC 2 cut(s) 15, 606
FalI AAGNNNNNCTT 2 cut(s) 992, 1024
FbaI TGATCA 2 cut(s) 121, 880
FblI GTMKAC 1 cut(s) 795
Fnu4HI GCNGC 3 cut(s) 352, 760, 1385
FokI GGATG 1 cut(s) 683
FriOI GRGCYC 2 cut(s) 15, 606
Fsp4HI GCNGC 3 cut(s) 352, 760, 1385
FspBI CTAG 4 cut(s) 105, 561, 1049, 1158
FspI TGCGCA 1 cut(s) 627
GlaI GCGC 1 cut(s) 627
GluI GCNGC 3 cut(s) 352, 760, 1385
GsaI CCCAGC 1 cut(s) 39
HaeIII GGCC 6 cut(s) 488, 507, 659, 721, 896, 1004
HapII CCGG 1 cut(s) 450
HhaI GCGC 1 cut(s) 628
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HpaII CCGG 1 cut(s) 450
HphI GGTGA 5 cut(s) 746, 953, 1023, 1181, 1366
Hpy166II GTNNAC 1 cut(s) 796
Hpy188I TCNGA 8 cut(s) 70, 91, 139, 330, 591, 853, 885, 1114
Hpy188III TCNNGA 5 cut(s) 230, 561, 653, 1219, 1431
Hpy8I GTNNAC 1 cut(s) 796
HpyCH4III ACNGT 2 cut(s) 56, 793
HpyCH4IV ACGT 1 cut(s) 491
HpyCH4V TGCA 5 cut(s) 743, 938, 954, 1100, 1384
HpyF10VI GCNNNNNNNGC 5 cut(s) 392, 601, 644, 944, 973
HpyF3I CTNAG 1 cut(s) 850
HpySE526I ACGT 1 cut(s) 491
HspAI GCGC 1 cut(s) 626
Ksp22I TGATCA 2 cut(s) 121, 880
Kzo9I GATC 4 cut(s) 19, 121, 880, 1215
LmnI GCTCC 3 cut(s) 168, 377, 600
Lsp1109I GCAGC 2 cut(s) 363, 1396
LweI GCATC 3 cut(s) 373, 582, 925
MaeI CTAG 4 cut(s) 105, 561, 1049, 1158
MaeII ACGT 1 cut(s) 491
MaeIII GTNAC 3 cut(s) 734, 778, 1153
MalI GATC 4 cut(s) 21, 123, 882, 1217
MbiI CCGCTC 1 cut(s) 812
MboI GATC 4 cut(s) 19, 121, 880, 1215
MfeI CAATTG 1 cut(s) 949
MhlI GDGCHC 2 cut(s) 15, 606
MlsI TGGCCA 2 cut(s) 488, 896
MluCI AATT 7 cut(s) 251, 307, 482, 862, 949, 1025, 1132
MluNI TGGCCA 2 cut(s) 488, 896
MlyI GAGTC 5 cut(s) 94, 188, 863, 1139, 1366
Mox20I TGGCCA 2 cut(s) 488, 896
MroXI GAANNNNTTC 2 cut(s) 1004, 1301
MscI TGGCCA 2 cut(s) 488, 896
MseI TTAA 6 cut(s) 356, 525, 618, 840, 1187, 1319
Msp20I TGGCCA 2 cut(s) 488, 896
MspA1I CMGCKG 2 cut(s) 554, 762
MspI CCGG 1 cut(s) 450
MunI CAATTG 1 cut(s) 949
MwoI GCNNNNNNNGC 5 cut(s) 392, 601, 644, 944, 973
NdeII GATC 4 cut(s) 19, 121, 880, 1215
NmuCI GTSAC 1 cut(s) 734
NsbI TGCGCA 1 cut(s) 627
NspI RCATGY 1 cut(s) 976
NspV TTCGAA 1 cut(s) 63
PaeR7I CTCGAG 1 cut(s) 653
PagI TCATGA 1 cut(s) 1430
PceI AGGCCT 2 cut(s) 507, 1004
PdmI GAANNNNTTC 2 cut(s) 1004, 1301
PfeI GAWTC 6 cut(s) 65, 71, 173, 325, 649, 958
PkrI GCNGC 3 cut(s) 353, 761, 1386
PleI GAGTC 5 cut(s) 94, 188, 862, 1139, 1365
PpsI GAGTC 5 cut(s) 94, 188, 862, 1139, 1365
Psp124BI GAGCTC 2 cut(s) 15, 606
PspEI GGTNACC 1 cut(s) 734
PspFI CCCAGC 1 cut(s) 35
PspPI GGNCC 1 cut(s) 139
PstNI CAGNNNCTG 1 cut(s) 380
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 2 cut(s) 109, 1053
RsaNI GTAC 2 cut(s) 108, 1052
SacI GAGCTC 2 cut(s) 15, 606
SalI GTCGAC 1 cut(s) 794
SaqAI TTAA 6 cut(s) 356, 525, 618, 840, 1187, 1319
SatI GCNGC 3 cut(s) 352, 760, 1385
Sau3AI GATC 4 cut(s) 19, 121, 880, 1215
Sau96I GGNCC 1 cut(s) 139
SchI GAGTC 5 cut(s) 94, 188, 863, 1139, 1366
SduI GDGCHC 2 cut(s) 15, 606
SfaNI GCATC 3 cut(s) 373, 582, 925
SfcI CTRYAG 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 653
SfuI TTCGAA 1 cut(s) 63
SinI GGWCC 1 cut(s) 139
SlaI CTCGAG 1 cut(s) 653
SmlI CTYRAG 4 cut(s) 501, 653, 773, 1338
SmoI CTYRAG 4 cut(s) 501, 653, 773, 1338
SpeI ACTAGT 1 cut(s) 1157
Sse9I AATT 7 cut(s) 251, 307, 482, 862, 949, 1025, 1132
SseBI AGGCCT 2 cut(s) 507, 1004
SsiI CCGC 2 cut(s) 760, 810
SspMI CTAG 4 cut(s) 105, 561, 1049, 1158
SstI GAGCTC 2 cut(s) 15, 606
StuI AGGCCT 2 cut(s) 507, 1004
TaaI ACNGT 2 cut(s) 56, 793
TaiI ACGT 1 cut(s) 494
TaqI TCGA 5 cut(s) 63, 296, 654, 795, 921
TaqII GACCGA 1 cut(s) 121
TasI AATT 7 cut(s) 251, 307, 482, 862, 949, 1025, 1132
TatI WGTACW 1 cut(s) 1051
TauI GCSGC 1 cut(s) 762
TfiI GAWTC 6 cut(s) 65, 71, 173, 325, 649, 958
Tru1I TTAA 6 cut(s) 356, 525, 618, 840, 1187, 1319
Tru9I TTAA 6 cut(s) 356, 525, 618, 840, 1187, 1319
TscAI CASTG 2 cut(s) 214, 722
TseFI GTSAC 1 cut(s) 734
TseI GCWGC 2 cut(s) 351, 1384
Tsp45I GTSAC 1 cut(s) 734
TspDTI ATGAA 5 cut(s) 500, 573, 671, 714, 1416
TspRI CASTG 2 cut(s) 214, 722
VpaK11BI GGWCC 1 cut(s) 139
XagI CCTNNNNNAGG 1 cut(s) 317
XapI RAATTY 1 cut(s) 251
XbaI TCTAGA 1 cut(s) 560
XceI RCATGY 1 cut(s) 976
XhoI CTCGAG 1 cut(s) 653
XmiI GTMKAC 1 cut(s) 795
XmnI GAANNNNTTC 2 cut(s) 1004, 1301
XspI CTAG 4 cut(s) 105, 561, 1049, 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.