MD01G1012300.v1.1

peptidyl-tRNA hydrolase 2, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006608
Physical Location & Seq
Forward (+)
6362546 .. 6364883
2338 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1012300.v1.1.491

Sequence Viewer

Length: 594 bp
ATGATGATGGGGTTGGAGTTGGGGTTGGGGTGGGTGGGTGCCATTTTGGTAGGAGCGGGCTGGCTTGCTTTGGGGTATTTCTTTGGCGCTCGTTACCCTCCTTCTCGCATTATTTTCTCAGCCAGACTCGCTAAGCAGGCTGCACTTGCCAATGATCTTAGCAATGGAAAGAAGAAGAAGAAGAAGAAGAACGAGGACAAGCCCAAAGATCCTCTCCAGATTGAAAACCTCGCCGACATTCTCCAAGATTTCAAAATGGTTTTGGTGGTCAGGAATGATCTAAAGATGGGTAAAGGGAAAATTGCCGCTCAATGCAGCCATGCGACTTTAGGCCTCTATAAAAAGGTCCTCCATCGAGCACCAAAAGCTTTAAACAGGTGGGAGATGTGTGCACAGCCTAAAGTTGTAGTGAAAATAGAAAGTGAAGAAGATATGCTAGTTTTGCAAGAAAGGGCTAAATCGCTGAATTTACCGACACACATTACAATTGATGCTGGCAGGACTCAGATTGCACCAAATTCAAGGACAGTGATGGCTATTCTTGGACCTGTTGAAGTGGTTGATGATGTAACAGGTGGACTGAAGCTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

198

Amino Acids

21.64

Weight (kDa)

9.72

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 84 - 197 1.6e-42 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AccBSI CCGCTC 2 cut(s) 56, 308
AciI CCGC 2 cut(s) 56, 306
AclWI GGATC 1 cut(s) 203
AcsI RAATTY 2 cut(s) 466, 517
AgsI TTSAA 4 cut(s) 224, 253, 522, 554
AluBI AGCT 2 cut(s) 368, 586
AluI AGCT 2 cut(s) 368, 586
Alw21I GWGCWC 2 cut(s) 361, 394
Alw44I GTGCAC 1 cut(s) 390
AlwI GGATC 1 cut(s) 203
AoxI GGCC 1 cut(s) 331
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 2 cut(s) 140, 315
ApoI RAATTY 2 cut(s) 466, 517
AspLEI GCGC 1 cut(s) 89
AspS9I GGNCC 2 cut(s) 346, 545
AvaII GGWCC 2 cut(s) 346, 545
BaeGI GKGCMC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 361, 394
BbvI GCAGC 2 cut(s) 127, 327
BccI CCATC 3 cut(s) 280, 360, 526
BfaI CTAG 1 cut(s) 437
BfoI RGCGCY 1 cut(s) 90
BisI GCNGC 3 cut(s) 141, 306, 316
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 3 cut(s) 142, 307, 317
Bme18I GGWCC 2 cut(s) 346, 545
BmgT120I GGNCC 2 cut(s) 346, 545
BmiI GGNNCC 1 cut(s) 40
BmsI GCATC 1 cut(s) 481
BpmI CTGGAG 1 cut(s) 200
Bpu1102I GCTNAGC 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bse3DI GCAATG 1 cut(s) 169
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 2 cut(s) 132, 518
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 2 cut(s) 127, 327
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 1 cut(s) 333
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 361, 394
BsnI GGCC 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 361, 394
Bsp143I GATC 3 cut(s) 154, 208, 277
Bsp1720I GCTNAGC 1 cut(s) 132
BspACI CCGC 2 cut(s) 56, 306
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 2 cut(s) 131, 517
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 1 cut(s) 203
BspT107I GGYRCC 1 cut(s) 38
BsrBI CCGCTC 2 cut(s) 56, 308
BsrDI GCAATG 1 cut(s) 169
BssMI GATC 3 cut(s) 154, 208, 277
Bst4CI ACNGT 1 cut(s) 529
BstC8I GCNNGC 5 cut(s) 58, 62, 66, 138, 496
BstDEI CTNAG 4 cut(s) 118, 132, 158, 504
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 3 cut(s) 157, 211, 280
BstMBI GATC 3 cut(s) 154, 208, 277
BstMWI GCNNNNNNNGC 5 cut(s) 128, 137, 146, 365, 442
BstSLI GKGCMC 1 cut(s) 394
BstV1I GCAGC 2 cut(s) 127, 327
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuRI GGCC 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 534
Cac8I GCNNGC 5 cut(s) 58, 62, 66, 138, 496
CfoI GCGC 1 cut(s) 89
Cfr13I GGNCC 2 cut(s) 346, 545
CviAII CATG 1 cut(s) 320
DdeI CTNAG 4 cut(s) 118, 132, 158, 504
DpnI GATC 3 cut(s) 156, 210, 279
DpnII GATC 3 cut(s) 154, 208, 277
DraI TTTAAA 1 cut(s) 372
Eco147I AGGCCT 1 cut(s) 333
Eco47I GGWCC 2 cut(s) 346, 545
EcoO109I RGGNCCY 1 cut(s) 346
FaeI CATG 1 cut(s) 323
FaiI YATR 3 cut(s) 321, 339, 434
FatI CATG 1 cut(s) 319
FauI CCCGC 1 cut(s) 49
Fnu4HI GCNGC 3 cut(s) 141, 306, 316
Fsp4HI GCNGC 3 cut(s) 141, 306, 316
FspBI CTAG 1 cut(s) 437
GlaI GCGC 1 cut(s) 88
GluI GCNGC 3 cut(s) 141, 306, 316
GsuI CTGGAG 1 cut(s) 200
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 1 cut(s) 333
HhaI GCGC 1 cut(s) 89
Hin1II CATG 1 cut(s) 323
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 2 cut(s) 126, 502
Hpy166II GTNNAC 2 cut(s) 392, 578
Hpy188I TCNGA 1 cut(s) 507
Hpy188III TCNNGA 2 cut(s) 217, 271
Hpy8I GTNNAC 2 cut(s) 392, 578
HpyAV CCTTC 1 cut(s) 111
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4V TGCA 5 cut(s) 143, 315, 392, 445, 512
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 137, 146, 365, 442
HpyF3I CTNAG 4 cut(s) 118, 132, 158, 504
Hsp92II CATG 1 cut(s) 323
HspAI GCGC 1 cut(s) 87
Kzo9I GATC 3 cut(s) 154, 208, 277
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 2 cut(s) 127, 327
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 437
MaeIII GTNAC 2 cut(s) 92, 568
MalI GATC 3 cut(s) 156, 210, 279
MbiI CCGCTC 2 cut(s) 56, 308
MboI GATC 3 cut(s) 154, 208, 277
MboII GAAGA 8 cut(s) 184, 187, 190, 193, 196, 199, 437, 440
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 2 cut(s) 361, 394
MluCI AATT 4 cut(s) 300, 466, 486, 517
MlyI GAGTC 2 cut(s) 120, 496
MnlI CCTC 6 cut(s) 108, 187, 222, 239, 344, 359
MseI TTAA 1 cut(s) 371
MunI CAATTG 1 cut(s) 486
MwoI GCNNNNNNNGC 5 cut(s) 128, 137, 146, 365, 442
NdeII GATC 3 cut(s) 154, 208, 277
NlaIII CATG 1 cut(s) 323
NlaIV GGNNCC 1 cut(s) 40
PceI AGGCCT 1 cut(s) 333
PkrI GCNGC 3 cut(s) 142, 307, 317
PleI GAGTC 2 cut(s) 120, 496
PpsI GAGTC 2 cut(s) 120, 496
PpuMI RGGWCCY 1 cut(s) 346
Psp5II RGGWCCY 1 cut(s) 346
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 2 cut(s) 346, 545
PspPPI RGGWCCY 1 cut(s) 346
PsuI RGATCY 1 cut(s) 208
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 3 cut(s) 141, 306, 316
Sau3AI GATC 3 cut(s) 154, 208, 277
Sau96I GGNCC 2 cut(s) 346, 545
SchI GAGTC 2 cut(s) 120, 496
SduI GDGCHC 2 cut(s) 361, 394
SetI ASST 7 cut(s) 231, 348, 370, 380, 550, 577, 588
SfaNI GCATC 1 cut(s) 481
SinI GGWCC 2 cut(s) 346, 545
Sse9I AATT 4 cut(s) 300, 466, 486, 517
SseBI AGGCCT 1 cut(s) 333
SsiI CCGC 2 cut(s) 56, 306
SspMI CTAG 1 cut(s) 437
StuI AGGCCT 1 cut(s) 333
TaaI ACNGT 1 cut(s) 529
TaqI TCGA 1 cut(s) 355
TasI AATT 4 cut(s) 300, 466, 486, 517
TauI GCSGC 1 cut(s) 308
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TscAI CASTG 1 cut(s) 534
TseI GCWGC 2 cut(s) 140, 315
TspRI CASTG 1 cut(s) 534
VneI GTGCAC 1 cut(s) 390
VpaK11BI GGWCC 2 cut(s) 346, 545
XapI RAATTY 2 cut(s) 466, 517
XspI CTAG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.