MD15G1337800.v1.1

peptidyl-tRNA hydrolase 2, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006608
Physical Location & Seq
Reverse (-)
38014749 .. 38018481
3733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1337800.v1.1.491

Sequence Viewer

Length: 588 bp
ATGCTGATGGGGTTGGGATGGGTGGGCGCCATTTTGGTAGGAGCTGGTTGGCTTGCTTTGGGGTATTGCTTTGGCGTGCGTTACCCTCCTGCTCGCATTATTTTCTCAGCCAGACTCGCTAAGCAAGCTGCACTTGCCAATGATTATAATAATGGCAAGAATAAGACGAAGAAGAATAAGCAGAACAAGGACAAGCCCAAAGACCCTCTAGAGATTGAAAACCTCGCCGACATTCTTGAAGATTTCAAAATGGTTTTGGTGGTCAGGAATGATCTAAAGATGGGTAAAGGGAAAATTGCCGCTCAATGCAGCCATGCAACTTTAGGCCTCTATAAAAAGGTCCTCCATCGAGCACCAAAAGCTTTAAACAGGTGGGAGATGTGTGCCCAGCCTAAAGTTGTAGTGAAAATAGAAAGTGAAAAAGATATGCTAGTTTTGCAAGAAAGGGCTAAATCGCTAAATTTACCGACACACATTACAATTGATGCTGGCAGGACTCAGATTGCACCAAATTCAAGGACAGTGATGGCTATTCTTGGACCTGTTGAAGTAGTTGATGATGTAACAGGTGGACTGAAGCTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

196

Amino Acids

21.46

Weight (kDa)

9.71

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 82 - 195 2.4e-42 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 147
AccB1I GGYRCC 1 cut(s) 26
AccBSI CCGCTC 1 cut(s) 302
AciI CCGC 1 cut(s) 300
AcsI RAATTY 2 cut(s) 460, 511
AcyI GRCGYC 1 cut(s) 27
AgsI TTSAA 5 cut(s) 218, 239, 247, 516, 548
AluBI AGCT 4 cut(s) 44, 128, 362, 580
AluI AGCT 4 cut(s) 44, 128, 362, 580
Alw21I GWGCWC 1 cut(s) 355
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 2 cut(s) 128, 309
ApoI RAATTY 2 cut(s) 460, 511
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 340, 539
AvaII GGWCC 2 cut(s) 340, 539
BaeGI GKGCMC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 355
BbvI GCAGC 2 cut(s) 115, 321
BccI CCATC 4 cut(s) 12, 274, 354, 520
BfaI CTAG 2 cut(s) 209, 431
BfoI RGCGCY 1 cut(s) 30
BisI GCNGC 3 cut(s) 129, 300, 310
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 3 cut(s) 130, 301, 311
Bme18I GGWCC 2 cut(s) 340, 539
BmgT120I GGNCC 2 cut(s) 340, 539
BmiI GGNNCC 1 cut(s) 28
BmsI GCATC 1 cut(s) 475
Bpu1102I GCTNAGC 1 cut(s) 120
BsaHI GRCGYC 1 cut(s) 27
BseGI GGATG 1 cut(s) 23
BseMII CTCAG 2 cut(s) 120, 512
BseSI GKGCMC 1 cut(s) 388
BseXI GCAGC 2 cut(s) 115, 321
BseYI CCCAGC 1 cut(s) 387
BsgI GTGCAG 1 cut(s) 114
BshFI GGCC 1 cut(s) 327
BshNI GGYRCC 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 355
BsnI GGCC 1 cut(s) 327
Bsp1286I GDGCHC 2 cut(s) 355, 388
Bsp143I GATC 1 cut(s) 271
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 1 cut(s) 300
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 2 cut(s) 119, 511
BspLI GGNNCC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 26
BsrBI CCGCTC 1 cut(s) 302
BssMI GATC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 27
Bst4CI ACNGT 1 cut(s) 523
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 5 cut(s) 54, 77, 94, 126, 490
BstDEI CTNAG 3 cut(s) 106, 120, 498
BstF5I GGATG 1 cut(s) 23
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 1 cut(s) 274
BstMBI GATC 1 cut(s) 271
BstMWI GCNNNNNNNGC 5 cut(s) 116, 125, 134, 359, 436
BstSLI GKGCMC 1 cut(s) 388
BstV1I GCAGC 2 cut(s) 115, 321
BsuRI GGCC 1 cut(s) 327
BtsCI GGATG 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 528
Cac8I GCNNGC 5 cut(s) 54, 77, 94, 126, 490
CfoI GCGC 1 cut(s) 29
Cfr13I GGNCC 2 cut(s) 340, 539
CviAII CATG 1 cut(s) 314
DdeI CTNAG 3 cut(s) 106, 120, 498
DinI GGCGCC 1 cut(s) 28
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
DraI TTTAAA 1 cut(s) 366
Eco147I AGGCCT 1 cut(s) 327
Eco47I GGWCC 2 cut(s) 340, 539
EcoO109I RGGNCCY 1 cut(s) 340
EgeI GGCGCC 1 cut(s) 28
EheI GGCGCC 1 cut(s) 28
FaeI CATG 1 cut(s) 317
FaiI YATR 4 cut(s) 147, 315, 333, 428
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FatI CATG 1 cut(s) 313
Fnu4HI GCNGC 3 cut(s) 129, 300, 310
FokI GGATG 1 cut(s) 30
Fsp4HI GCNGC 3 cut(s) 129, 300, 310
FspBI CTAG 2 cut(s) 209, 431
GlaI GCGC 1 cut(s) 28
GluI GCNGC 3 cut(s) 129, 300, 310
GsaI CCCAGC 1 cut(s) 391
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 1 cut(s) 327
HhaI GCGC 1 cut(s) 29
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 1 cut(s) 317
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HindIII AAGCTT 1 cut(s) 360
HinfI GANTC 2 cut(s) 114, 496
Hpy166II GTNNAC 1 cut(s) 572
Hpy188I TCNGA 1 cut(s) 501
Hpy188III TCNNGA 3 cut(s) 209, 236, 265
Hpy8I GTNNAC 1 cut(s) 572
HpyCH4III ACNGT 1 cut(s) 523
HpyCH4V TGCA 5 cut(s) 131, 309, 317, 439, 506
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 125, 134, 359, 436
HpyF3I CTNAG 3 cut(s) 106, 120, 498
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 1 cut(s) 317
HspAI GCGC 1 cut(s) 27
KasI GGCGCC 1 cut(s) 26
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 1 cut(s) 41
Lsp1109I GCAGC 2 cut(s) 115, 321
LweI GCATC 1 cut(s) 475
MaeI CTAG 2 cut(s) 209, 431
MaeIII GTNAC 2 cut(s) 80, 562
MalI GATC 1 cut(s) 273
MbiI CCGCTC 1 cut(s) 302
MboI GATC 1 cut(s) 271
MboII GAAGA 3 cut(s) 181, 184, 251
MfeI CAATTG 1 cut(s) 480
MhlI GDGCHC 2 cut(s) 355, 388
MluCI AATT 4 cut(s) 294, 460, 480, 511
Mly113I GGCGCC 1 cut(s) 27
MlyI GAGTC 2 cut(s) 108, 490
MnlI CCTC 5 cut(s) 96, 216, 233, 338, 353
MseI TTAA 1 cut(s) 365
MunI CAATTG 1 cut(s) 480
MwoI GCNNNNNNNGC 5 cut(s) 116, 125, 134, 359, 436
NarI GGCGCC 1 cut(s) 27
NdeII GATC 1 cut(s) 271
NlaIII CATG 1 cut(s) 317
NlaIV GGNNCC 1 cut(s) 28
PceI AGGCCT 1 cut(s) 327
PkrI GCNGC 3 cut(s) 130, 301, 311
PleI GAGTC 2 cut(s) 108, 490
PluTI GGCGCC 1 cut(s) 30
PpsI GAGTC 2 cut(s) 108, 490
PpuMI RGGWCCY 1 cut(s) 340
PsiI TTATAA 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 387
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 2 cut(s) 340, 539
PspPPI RGGWCCY 1 cut(s) 340
SaqAI TTAA 1 cut(s) 365
SatI GCNGC 3 cut(s) 129, 300, 310
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 2 cut(s) 340, 539
SchI GAGTC 2 cut(s) 108, 490
SduI GDGCHC 2 cut(s) 355, 388
SetI ASST 9 cut(s) 46, 130, 225, 342, 364, 374, 544, 571, 582
SfaNI GCATC 1 cut(s) 475
SfoI GGCGCC 1 cut(s) 28
SinI GGWCC 2 cut(s) 340, 539
Sse9I AATT 4 cut(s) 294, 460, 480, 511
SseBI AGGCCT 1 cut(s) 327
SsiI CCGC 1 cut(s) 300
SspDI GGCGCC 1 cut(s) 26
SspMI CTAG 2 cut(s) 209, 431
StuI AGGCCT 1 cut(s) 327
TaaI ACNGT 1 cut(s) 523
TaqI TCGA 1 cut(s) 349
TasI AATT 4 cut(s) 294, 460, 480, 511
TauI GCSGC 1 cut(s) 302
Tru1I TTAA 1 cut(s) 365
Tru9I TTAA 1 cut(s) 365
TscAI CASTG 1 cut(s) 528
TseI GCWGC 2 cut(s) 128, 309
TspRI CASTG 1 cut(s) 528
VpaK11BI GGWCC 2 cut(s) 340, 539
XapI RAATTY 2 cut(s) 460, 511
XbaI TCTAGA 1 cut(s) 208
XspI CTAG 2 cut(s) 209, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.