Rh1CG442500

peptidyl-tRNA hydrolase 2, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0006608
Physical Location & Seq
Forward (+)
69534152 .. 69536727
2576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG442500.1

Sequence Viewer

Length: 543 bp
ATGATGGGTTTGGGTTGGGTGGGAGCGATATTAGTAGGAGCAGGCTGGCTTGCTTTGGGCTATTGCTTTGGCGCGCGTTACCCTCCTTCTCGCATTTTGGTCTCAGCTAGAGCCGCCAGACTAGCCAAGCAGAAAGCTGTTGCCAGTGACGGTAACGGCAAGAAGAAGAAGATCAAAGACCCCCTTGACATTGAAAAGCTCGCTGACATTCTCCAAGATTTCAAAATGGTCTTGGTGGTCAGGAATGATCTGAAGATGGGCAAAGGGAAAATTGCTGCTCAATGCAGTCATGCGACTTTGGGCCTCTACAAAAAGCTCCTCCGTCGGGCACCAAAATCTTTAAACAGGTGGGAGATGTGTGCACAACCTAAAGTTGTTCTAAAAGTAGAAACTGAAGAAGAGATGCTTGTTTTGCAAGATAGAGCAAAATCACTTAACTTACCAAACCATATTACAATTGATGCTGGGAAGACTCAGATTGCACCAAGTGAGTTGAGAGTGATTGGTTTTGTTATTTTTGAGGTTTTGGTAATGTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

180

Amino Acids

19.87

Weight (kDa)

9.86

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 74 - 169 5.5e-34 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AccII CGCG 2 cut(s) 74, 76
AciI CCGC 1 cut(s) 114
AcuI CTGAAG 2 cut(s) 272, 414
AdeI CACNNNGTG 1 cut(s) 488
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 2 cut(s) 194, 223
AluBI AGCT 4 cut(s) 107, 137, 199, 316
AluI AGCT 4 cut(s) 107, 137, 199, 316
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 1 cut(s) 106
Alw44I GTGCAC 1 cut(s) 360
AoxI GGCC 1 cut(s) 301
ApaLI GTGCAC 1 cut(s) 360
ApeKI GCWGC 1 cut(s) 275
AspLEI GCGC 2 cut(s) 74, 76
AspS9I GGNCC 1 cut(s) 301
BaeGI GKGCMC 2 cut(s) 331, 364
BanI GGYRCC 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 1 cut(s) 262
BccI CCATC 1 cut(s) 250
BceAI ACGGC 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 2 cut(s) 108, 122
BisI GCNGC 2 cut(s) 114, 276
BlsI GCNGC 2 cut(s) 115, 277
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 330
BmsI GCATC 2 cut(s) 393, 451
BpiI GAAGAC 1 cut(s) 476
BsaI GGTCTC 1 cut(s) 106
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMII CTCAG 2 cut(s) 117, 488
BseNI ACTGG 1 cut(s) 144
BsePI GCGCGC 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 308
BseSI GKGCMC 2 cut(s) 331, 364
BseXI GCAGC 1 cut(s) 262
BseYI CCCAGC 1 cut(s) 464
Bsh1236I CGCG 2 cut(s) 74, 76
BshFI GGCC 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 325
BsmAI GTCTC 1 cut(s) 106
BsnI GGCC 1 cut(s) 303
Bso31I GGTCTC 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 331, 364
Bsp143I GATC 2 cut(s) 171, 247
BspACI CCGC 1 cut(s) 114
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 2 cut(s) 116, 487
BspFNI CGCG 2 cut(s) 74, 76
BspLI GGNNCC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 106
BsrI ACTGG 1 cut(s) 144
BssHII GCGCGC 1 cut(s) 72
BssMI GATC 2 cut(s) 171, 247
Bst4CI ACNGT 1 cut(s) 152
Bst6I CTCTTC 1 cut(s) 393
BstC8I GCNNGC 5 cut(s) 43, 47, 51, 74, 201
BstDEI CTNAG 2 cut(s) 103, 474
BstFNI CGCG 2 cut(s) 74, 76
BstHHI GCGC 2 cut(s) 74, 76
BstKTI GATC 2 cut(s) 174, 250
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 2 cut(s) 171, 247
BstMWI GCNNNNNNNGC 3 cut(s) 113, 122, 412
BstSLI GKGCMC 2 cut(s) 331, 364
BstUI CGCG 2 cut(s) 74, 76
BstV1I GCAGC 1 cut(s) 262
BstV2I GAAGAC 1 cut(s) 476
BsuRI GGCC 1 cut(s) 303
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 5 cut(s) 43, 47, 51, 74, 201
CfoI GCGC 2 cut(s) 74, 76
Cfr13I GGNCC 1 cut(s) 301
CviAII CATG 1 cut(s) 290
DdeI CTNAG 2 cut(s) 103, 474
DpnI GATC 2 cut(s) 173, 249
DpnII GATC 2 cut(s) 171, 247
DraI TTTAAA 1 cut(s) 342
DraIII CACNNNGTG 1 cut(s) 488
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco31I GGTCTC 1 cut(s) 106
Eco57I CTGAAG 2 cut(s) 272, 414
FaeI CATG 1 cut(s) 293
FaiI YATR 2 cut(s) 291, 450
FalI AAGNNNNNCTT 4 cut(s) 168, 200, 390, 422
FatI CATG 1 cut(s) 289
Fnu4HI GCNGC 2 cut(s) 114, 276
Fsp4HI GCNGC 2 cut(s) 114, 276
FspBI CTAG 2 cut(s) 108, 122
GlaI GCGC 2 cut(s) 73, 75
GluI GCNGC 2 cut(s) 114, 276
GsaI CCCAGC 1 cut(s) 468
HaeIII GGCC 1 cut(s) 303
HhaI GCGC 2 cut(s) 74, 76
Hin1II CATG 1 cut(s) 293
Hin6I GCGC 2 cut(s) 72, 74
HinP1I GCGC 2 cut(s) 72, 74
HinfI GANTC 1 cut(s) 472
Hpy166II GTNNAC 1 cut(s) 362
Hpy188I TCNGA 2 cut(s) 252, 477
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 362
Hpy99I CGWCG 1 cut(s) 327
HpyAV CCTTC 1 cut(s) 96
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4V TGCA 4 cut(s) 285, 362, 415, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 122, 412
HpyF3I CTNAG 2 cut(s) 103, 474
Hsp92II CATG 1 cut(s) 293
HspAI GCGC 2 cut(s) 72, 74
Kzo9I GATC 2 cut(s) 171, 247
LmnI GCTCC 3 cut(s) 23, 38, 321
LpnPI CCDG 7 cut(s) 27, 31, 130, 157, 226, 331, 450
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 2 cut(s) 393, 451
MaeI CTAG 2 cut(s) 108, 122
MaeIII GTNAC 3 cut(s) 77, 146, 152
MalI GATC 2 cut(s) 173, 249
MboI GATC 2 cut(s) 171, 247
MboII GAAGA 7 cut(s) 175, 178, 181, 265, 407, 410, 481
MfeI CAATTG 1 cut(s) 456
MhlI GDGCHC 2 cut(s) 331, 364
MluCI AATT 2 cut(s) 270, 456
MlyI GAGTC 1 cut(s) 466
MnlI CCTC 4 cut(s) 93, 314, 329, 514
MseI TTAA 2 cut(s) 341, 435
MunI CAATTG 1 cut(s) 456
MvnI CGCG 2 cut(s) 74, 76
MwoI GCNNNNNNNGC 3 cut(s) 113, 122, 412
NdeII GATC 2 cut(s) 171, 247
NlaIII CATG 1 cut(s) 293
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 1 cut(s) 146
PauI GCGCGC 1 cut(s) 72
PkrI GCNGC 2 cut(s) 115, 277
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PspFI CCCAGC 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 1 cut(s) 301
PteI GCGCGC 1 cut(s) 72
SaqAI TTAA 2 cut(s) 341, 435
SatI GCNGC 2 cut(s) 114, 276
Sau3AI GATC 2 cut(s) 171, 247
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 1 cut(s) 466
SduI GDGCHC 2 cut(s) 331, 364
SetI ASST 7 cut(s) 109, 139, 201, 318, 350, 370, 525
SfaNI GCATC 2 cut(s) 393, 451
Sse9I AATT 2 cut(s) 270, 456
SsiI CCGC 1 cut(s) 114
SspMI CTAG 2 cut(s) 108, 122
TaaI ACNGT 1 cut(s) 152
TasI AATT 2 cut(s) 270, 456
TauI GCSGC 1 cut(s) 116
Tru1I TTAA 2 cut(s) 341, 435
Tru9I TTAA 2 cut(s) 341, 435
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 146
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 1 cut(s) 151
VneI GTGCAC 1 cut(s) 360
XspI CTAG 2 cut(s) 108, 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.