MD01G1029000.v1.1

Protein of unknown function (DUF4057)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009044
Physical Location & Seq
Forward (+)
10614515 .. 10616839
2325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1029000.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGTCAATAACATTTTTTGTTTATTTATTTATTATCTGCTATTTTCTAATCATCCAAAAGCCAAACGGGGAAGAGGGACAGAGGAAGAGAGTGAGGCCGAAAACAATGGAGAGAACAGCACCGAGAAAGCCTCGCAGAGACACTGCCGATATGCTGACGTGGAAAGAGGTCCCACACTCCGACTCTCCGGCCACCGCCTCCTCCGCCCACCGCTCTCATCAGCCGTCCGATGGAATCAGCAAGGTGCTCCATGGCGGTCAGATCACTGATGAAGAAGCTCAGAGCCTCAACAAACAGAAGCCTTGTTCAGGCCATAAATTGAAGGAGATTACTGGAAATGGTATATTTGCAGCTGGGTCAGGCAAGGATGCTGCACCAGAATCTAATGATGCTAGTAACAAAACGGGGCTTCGTATTTATCAGCAAGCACTCAATGGAGTCAGTCAAATCTCATTCAGTGTTGATGAACATGTCACGCCCAAGAAGCCTGTCACTGTTCCTGAGGTTGCCAAGCAGCGTGAATTGAGTGGGACATTGCAGTCCGACTCAGATTCAAAATCCAAGAAGAGCGTATCAAATGCCAAGTCCAAGGAACTCACTGGAAATGACATCTTTGGCCCTCCTCCTGAAGTCTTGCCTCGATCATTGGCTGCTGCTCACACCCTGCAAACTAGACAAACCAGAGACATGGGGGAACCTGCACCCCGAAATCTGCGCACCTCTGTCAAAGTTTCCAATCCTGCTGGAGGTCAGAGTAATATTATGTTCAGTGAGGAACCTGTTTCCAAGACTCCAAGGAAAATACATAACCAGAAGTTTGCGGAGCTGACGGGGAATGATATATTTAAGGGAGATGTTCCTCCAGGATCTGCTGAAAAGCCCCTGAGCACGGCTAAGCTGAGAGAGATGAATGGCAGCAACATCTTTGCTGATGGAAAGGCATCATCCAGAGACTATCTGGGTGGTGTTCGTAAGCCCCCCGGTGGAGAAAGCAGCATCGCCTTGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4057

Protein Analysis

337

Amino Acids

36.37

Weight (kDa)

9.6

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4057 PF13266 39 - 334 1.3e-146 Protein of unknown function (DUF4057)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 538
Acc16I TGCGCA 1 cut(s) 716
Acc36I ACCTGC 1 cut(s) 706
AccBSI CCGCTC 1 cut(s) 213
AciI CCGC 5 cut(s) 195, 204, 211, 255, 821
AclWI GGATC 1 cut(s) 874
AcoI YGGCCR 1 cut(s) 189
AcuI CTGAAG 1 cut(s) 648
AfiI CCNNNNNNNGG 5 cut(s) 230, 308, 746, 889, 983
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 2 cut(s) 322, 555
AjiI CACGTC 1 cut(s) 159
AjnI CCWGG 1 cut(s) 862
AluBI AGCT 4 cut(s) 278, 353, 826, 898
AluI AGCT 4 cut(s) 278, 353, 826, 898
Alw21I GWGCWC 2 cut(s) 249, 890
Alw26I GTCTC 3 cut(s) 132, 678, 945
AlwI GGATC 1 cut(s) 874
AlwNI CAGNNNCTG 1 cut(s) 869
AoxI GGCC 4 cut(s) 95, 189, 310, 616
ApeKI GCWGC 7 cut(s) 350, 371, 514, 650, 653, 915, 993
ArsI GACNNNNNNTTYG 2 cut(s) 569, 601
AspLEI GCGC 1 cut(s) 717
AspS9I GGNCC 2 cut(s) 169, 617
AsuC2I CCSGG 1 cut(s) 981
AvaII GGWCC 1 cut(s) 169
AxyI CCTNAGG 1 cut(s) 501
Bbv12I GWGCWC 2 cut(s) 249, 890
BbvI GCAGC 7 cut(s) 358, 362, 526, 637, 640, 927, 1005
BccI CCATC 2 cut(s) 224, 926
BceAI ACGGC 2 cut(s) 208, 906
BciT130I CCWGG 1 cut(s) 864
BcnI CCSGG 1 cut(s) 981
BcoDI GTCTC 3 cut(s) 132, 678, 945
BfaI CTAG 2 cut(s) 393, 672
BfuAI ACCTGC 1 cut(s) 706
BisI GCNGC 7 cut(s) 351, 372, 515, 651, 654, 916, 994
BlpI GCTNAGC 1 cut(s) 894
BlsI GCNGC 7 cut(s) 352, 373, 516, 652, 655, 917, 995
Bme1390I CCNGG 2 cut(s) 864, 981
Bme18I GGWCC 1 cut(s) 169
BmgBI CACGTC 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 169, 617
BmiI GGNNCC 3 cut(s) 171, 696, 777
BmrFI CCNGG 2 cut(s) 864, 981
BmsI GCATC 4 cut(s) 358, 379, 950, 1005
BplI GAGNNNNNCTC 2 cut(s) 115, 147
BpmI CTGGAG 2 cut(s) 765, 846
Bpu10I CCTNAGC 1 cut(s) 884
Bpu1102I GCTNAGC 1 cut(s) 894
BpuMI CCSGG 1 cut(s) 981
BsaJI CCNNGG 5 cut(s) 250, 588, 794, 979, 1002
Bsc4I CCNNNNNNNGG 5 cut(s) 230, 308, 746, 889, 983
Bse1I ACTGG 2 cut(s) 337, 604
Bse21I CCTNAGG 1 cut(s) 501
Bse3DI GCAATG 1 cut(s) 533
BseBI CCWGG 1 cut(s) 864
BseDI CCNNGG 5 cut(s) 250, 588, 794, 979, 1002
BseGI GGATG 3 cut(s) 51, 373, 944
BseLI CCNNNNNNNGG 5 cut(s) 230, 308, 746, 889, 983
BseMI GCAATG 1 cut(s) 533
BseMII CTCAG 5 cut(s) 293, 492, 561, 875, 890
BseNI ACTGG 2 cut(s) 337, 604
BseRI GAGGAG 2 cut(s) 190, 612
BseXI GCAGC 7 cut(s) 358, 362, 526, 637, 640, 927, 1005
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 2 cut(s) 357, 684
BshFI GGCC 4 cut(s) 97, 191, 312, 618
BsiHKAI GWGCWC 2 cut(s) 249, 890
BsiSI CCGG 2 cut(s) 188, 981
BslFI GGGAC 3 cut(s) 90, 155, 544
BslI CCNNNNNNNGG 5 cut(s) 230, 308, 746, 889, 983
BsmAI GTCTC 3 cut(s) 132, 678, 945
BsmFI GGGAC 3 cut(s) 90, 155, 544
BsnI GGCC 4 cut(s) 97, 191, 312, 618
Bsp1286I GDGCHC 2 cut(s) 249, 890
Bsp143I GATC 3 cut(s) 261, 641, 866
Bsp1720I GCTNAGC 1 cut(s) 894
Bsp19I CCATGG 1 cut(s) 250
BspACI CCGC 5 cut(s) 195, 204, 211, 255, 821
BspANI GGCC 4 cut(s) 97, 191, 312, 618
BspCNI CTCAG 5 cut(s) 292, 493, 560, 876, 891
BspLI GGNNCC 3 cut(s) 171, 696, 777
BspMI ACCTGC 1 cut(s) 706
BspPI GGATC 1 cut(s) 874
BspQI GCTCTTC 1 cut(s) 560
BsrBI CCGCTC 1 cut(s) 213
BsrDI GCAATG 1 cut(s) 533
BsrI ACTGG 2 cut(s) 337, 604
BssECI CCNNGG 5 cut(s) 250, 588, 794, 979, 1002
BssMI GATC 3 cut(s) 261, 641, 866
BssT1I CCWWGG 4 cut(s) 250, 588, 794, 1002
Bst2UI CCWGG 1 cut(s) 864
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 3 cut(s) 66, 80, 560
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 6 cut(s) 279, 501, 547, 884, 894, 899
BstDSI CCRYGG 1 cut(s) 250
BstENI CCTNNNNNAGG 2 cut(s) 306, 744
BstF5I GGATG 3 cut(s) 51, 373, 944
BstHHI GCGC 1 cut(s) 717
BstKTI GATC 3 cut(s) 264, 644, 869
BstMAI GTCTC 3 cut(s) 132, 678, 945
BstMBI GATC 3 cut(s) 261, 641, 866
BstMWI GCNNNNNNNGC 2 cut(s) 203, 484
BstNI CCWGG 1 cut(s) 864
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 862, 979
BstV1I GCAGC 7 cut(s) 358, 362, 526, 637, 640, 927, 1005
BstX2I RGATCY 1 cut(s) 866
BstXI CCANNNNNNTGG 1 cut(s) 688
BstYI RGATCY 1 cut(s) 866
Bsu36I CCTNAGG 1 cut(s) 501
BsuRI GGCC 4 cut(s) 97, 191, 312, 618
BtgI CCRYGG 1 cut(s) 250
BtgZI GCGATG 1 cut(s) 982
BtrI CACGTC 1 cut(s) 159
BtsCI GGATG 3 cut(s) 51, 373, 944
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 6 cut(s) 141, 264, 463, 492, 597, 775
BveI ACCTGC 1 cut(s) 706
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 869
CfoI GCGC 1 cut(s) 717
Cfr13I GGNCC 2 cut(s) 169, 617
CviAII CATG 3 cut(s) 251, 470, 688
DdeI CTNAG 6 cut(s) 279, 501, 547, 884, 894, 899
DpnI GATC 3 cut(s) 263, 643, 868
DpnII GATC 3 cut(s) 261, 641, 866
DrdI GACNNNNNNGTC 1 cut(s) 538
DseDI GACNNNNNNGTC 1 cut(s) 538
EaeI YGGCCR 1 cut(s) 189
Eam1104I CTCTTC 3 cut(s) 66, 80, 560
EarI CTCTTC 3 cut(s) 66, 80, 560
EciI GGCGGA 1 cut(s) 193
Eco130I CCWWGG 4 cut(s) 250, 588, 794, 1002
Eco47I GGWCC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 648
Eco81I CCTNAGG 1 cut(s) 501
EcoNI CCTNNNNNAGG 2 cut(s) 306, 744
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 862
EcoT14I CCWWGG 4 cut(s) 250, 588, 794, 1002
ErhI CCWWGG 4 cut(s) 250, 588, 794, 1002
FaeI CATG 3 cut(s) 254, 473, 691
FaiI YATR 9 cut(s) 152, 252, 315, 344, 471, 689, 764, 807, 842
FaqI GGGAC 3 cut(s) 90, 155, 544
FatI CATG 3 cut(s) 250, 469, 687
Fnu4HI GCNGC 7 cut(s) 351, 372, 515, 651, 654, 916, 994
FokI GGATG 3 cut(s) 38, 380, 931
Fsp4HI GCNGC 7 cut(s) 351, 372, 515, 651, 654, 916, 994
FspBI CTAG 2 cut(s) 393, 672
FspI TGCGCA 1 cut(s) 716
GlaI GCGC 1 cut(s) 716
GluI GCNGC 7 cut(s) 351, 372, 515, 651, 654, 916, 994
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 2 cut(s) 765, 846
HaeIII GGCC 4 cut(s) 97, 191, 312, 618
HapII CCGG 2 cut(s) 188, 981
HhaI GCGC 1 cut(s) 717
Hin1II CATG 3 cut(s) 254, 473, 691
Hin6I GCGC 1 cut(s) 715
HinP1I GCGC 1 cut(s) 715
HinfI GANTC 7 cut(s) 182, 234, 380, 438, 545, 551, 790
HpaII CCGG 2 cut(s) 188, 981
Hpy188I TCNGA 7 cut(s) 181, 229, 261, 282, 544, 550, 753
Hpy188III TCNNGA 3 cut(s) 500, 626, 948
HpyAV CCTTC 1 cut(s) 316
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 5 cut(s) 350, 374, 538, 667, 701
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 484
HpyF3I CTNAG 6 cut(s) 279, 501, 547, 884, 894, 899
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 3 cut(s) 254, 473, 691
HspAI GCGC 1 cut(s) 715
Kzo9I GATC 3 cut(s) 261, 641, 866
LguI GCTCTTC 1 cut(s) 560
LmnI GCTCC 2 cut(s) 252, 823
Lsp1109I GCAGC 7 cut(s) 358, 362, 526, 637, 640, 927, 1005
LweI GCATC 4 cut(s) 358, 379, 950, 1005
MaeI CTAG 2 cut(s) 393, 672
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 3 cut(s) 395, 472, 490
MalI GATC 3 cut(s) 263, 643, 868
MbiI CCGCTC 1 cut(s) 213
MboI GATC 3 cut(s) 261, 641, 866
MboII GAAGA 4 cut(s) 83, 97, 284, 577
MflI RGATCY 1 cut(s) 866
MhlI GDGCHC 2 cut(s) 249, 890
MluCI AATT 2 cut(s) 317, 521
MlyI GAGTC 4 cut(s) 176, 447, 539, 784
MmeI TCCRAC 2 cut(s) 204, 567
MseI TTAA 1 cut(s) 846
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 2 cut(s) 188, 981
MspR9I CCNGG 2 cut(s) 864, 981
MvaI CCWGG 1 cut(s) 864
MwoI GCNNNNNNNGC 2 cut(s) 203, 484
NciI CCSGG 1 cut(s) 981
NcoI CCATGG 1 cut(s) 250
NdeII GATC 3 cut(s) 261, 641, 866
NlaIII CATG 3 cut(s) 254, 473, 691
NlaIV GGNNCC 3 cut(s) 171, 696, 777
NmuCI GTSAC 2 cut(s) 472, 490
NsbI TGCGCA 1 cut(s) 716
NspI RCATGY 1 cut(s) 473
PciI ACATGT 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 560
PfeI GAWTC 3 cut(s) 234, 380, 551
PfoI TCCNGGA 1 cut(s) 862
PkrI GCNGC 7 cut(s) 352, 373, 516, 652, 655, 917, 995
PleI GAGTC 4 cut(s) 176, 446, 539, 784
PpsI GAGTC 4 cut(s) 176, 446, 539, 784
PpuMI RGGWCCY 1 cut(s) 169
PscI ACATGT 1 cut(s) 469
Psp5II RGGWCCY 1 cut(s) 169
Psp6I CCWGG 1 cut(s) 862
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 862
PspN4I GGNNCC 3 cut(s) 171, 696, 777
PspPI GGNCC 2 cut(s) 169, 617
PspPPI RGGWCCY 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 1 cut(s) 866
PvuII CAGCTG 1 cut(s) 353
SapI GCTCTTC 1 cut(s) 560
SaqAI TTAA 1 cut(s) 846
SatI GCNGC 7 cut(s) 351, 372, 515, 651, 654, 916, 994
Sau3AI GATC 3 cut(s) 261, 641, 866
Sau96I GGNCC 2 cut(s) 169, 617
SchI GAGTC 4 cut(s) 176, 447, 539, 784
ScrFI CCNGG 2 cut(s) 864, 981
SduI GDGCHC 2 cut(s) 249, 890
SfaNI GCATC 4 cut(s) 358, 379, 950, 1005
SinI GGWCC 1 cut(s) 169
Sse9I AATT 2 cut(s) 317, 521
SsiI CCGC 5 cut(s) 195, 204, 211, 255, 821
SspI AATATT 1 cut(s) 760
SspMI CTAG 2 cut(s) 393, 672
StyD4I CCNGG 2 cut(s) 862, 979
StyI CCWWGG 4 cut(s) 250, 588, 794, 1002
TaaI ACNGT 1 cut(s) 496
TaiI ACGT 1 cut(s) 161
TaqI TCGA 1 cut(s) 640
TasI AATT 2 cut(s) 317, 521
TfiI GAWTC 3 cut(s) 234, 380, 551
Tru1I TTAA 1 cut(s) 846
Tru9I TTAA 1 cut(s) 846
TscAI CASTG 6 cut(s) 148, 271, 463, 499, 604, 775
TseFI GTSAC 2 cut(s) 472, 490
TseI GCWGC 7 cut(s) 350, 371, 514, 650, 653, 915, 993
Tsp45I GTSAC 2 cut(s) 472, 490
TspDTI ATGAA 3 cut(s) 285, 480, 923
TspRI CASTG 6 cut(s) 148, 271, 463, 499, 604, 775
VpaK11BI GGWCC 1 cut(s) 169
XagI CCTNNNNNAGG 2 cut(s) 306, 744
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XspI CTAG 2 cut(s) 393, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.