RLG00000018101

Protein of unknown function (DUF4057)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009044
Physical Location & Seq
Reverse (-)
25325694 .. 25327265
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018101

Sequence Viewer

Length: 876 bp
ATGGCCACACCAGTGAGAAAGCCTCGCCGCGACACCGCCGACATGCTGACGTGGCAAGAGGTCCCCAGCTCCGACTCTCCCGCCACCGCCTCCTCCGCTCACCGCTCTCACCAGCCGTCGGATGGGATCAGCAAGGTGCTTCACGGCGGTCAGATCACCGAGGAAGAAGCTCAGAGCCTGAACAAGAAGAAGCCTTGCTCCGGGTACAAAATGAAGGAGATTTCCGGAAGCGGGATATTCGCGGCTGGAGAAGGCAATGCGGAGCCGGAGCCGGGTCGGGTTTACCAGCAGGCGGTGAAAGGGATCAGTCAAATCTCGTTCAGCGTTGACGAGCACGTGAGCCCTAAGAAGCCGACGAGTGTTCCGGAGGTGGCGAAGCAGAGGGAGCTGAGTGGGACTTTGCAGTCGGAATTGGATTCCAAGACTCAGAAGGGGATTTCAAATGCCAAGACCAAGGAGCTTAGTGGAAATGACATCTTTGGCCCTCCCCCGGAAATTGTTCCCCGATCGGTTAATGCTGTTCACACCCACGAGTATAAGCAGAGTAAAGATTCCGCGGAGCCGGCGCCCAGAAATCTCCGCACCTCTGTCAAAGTTTCCAATCCTGCTGGAGGTCAGAGTAAAATCTTGTTCACTGAGGACCCTGTTGTCAAGACTTCCAAGAAAATTCATAACCAGAAGTTTGCAGAGCTGACAGGCAATGACATATTTAAGGGAGATGTTTCTCCAGGATCTGCTGAAAAGCCACTAAGCACGGCTAAGCTGAGAGAAATGAGTGGCAGCAACATCTTTGCTGATGGAAAGGCGGCATCAAGAGACTATCTAGGTGGTGTTCGCAAGCCCCCTGGTGGCGAGAGCAGCATATCATTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4057

Protein Analysis

292

Amino Acids

31.05

Weight (kDa)

9.2

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4057 PF13266 2 - 289 3.9e-144 Protein of unknown function (DUF4057)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 403, 647
AccB1I GGYRCC 1 cut(s) 565
AccBSI CCGCTC 2 cut(s) 98, 105
AccII CGCG 3 cut(s) 30, 242, 557
AccIII TCCGGA 2 cut(s) 224, 364
AclWI GGATC 3 cut(s) 134, 311, 739
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 666
AcvI CACGTG 1 cut(s) 337
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 9 cut(s) 118, 122, 200, 231, 272, 292, 490, 611, 848
AgsI TTSAA 1 cut(s) 441
AjiI CACGTC 1 cut(s) 51
AjnI CCWGG 2 cut(s) 727, 844
AleI CACNNNNGTG 1 cut(s) 11
AluBI AGCT 6 cut(s) 69, 170, 388, 460, 691, 763
AluI AGCT 6 cut(s) 69, 170, 388, 460, 691, 763
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 1 cut(s) 810
AlwI GGATC 3 cut(s) 134, 311, 739
AlwNI CAGNNNCTG 2 cut(s) 178, 734
Aor13HI TCCGGA 2 cut(s) 224, 364
AoxI GGCC 2 cut(s) 3, 481
ApeKI GCWGC 2 cut(s) 780, 858
ApoI RAATTY 1 cut(s) 666
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 568
AspS9I GGNCC 3 cut(s) 61, 482, 640
AsuC2I CCSGG 3 cut(s) 202, 273, 491
AsuHPI GGTGA 4 cut(s) 92, 101, 148, 307
AvaII GGWCC 2 cut(s) 61, 640
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 344
BauI CACGAG 1 cut(s) 530
BbrPI CACGTG 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 2 cut(s) 792, 870
BccI CCATC 2 cut(s) 116, 791
BceAI ACGGC 3 cut(s) 100, 160, 771
BciT130I CCWGG 2 cut(s) 729, 846
BcnI CCSGG 3 cut(s) 202, 273, 491
BcoDI GTCTC 1 cut(s) 810
BfaI CTAG 1 cut(s) 824
BfoI RGCGCY 1 cut(s) 569
BisI GCNGC 5 cut(s) 28, 243, 781, 807, 859
BlpI GCTNAGC 1 cut(s) 759
BlsI GCNGC 5 cut(s) 29, 244, 782, 808, 860
Bme1390I CCNGG 5 cut(s) 202, 273, 491, 729, 846
Bme18I GGWCC 2 cut(s) 61, 640
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 3 cut(s) 61, 482, 640
BmiI GGNNCC 6 cut(s) 63, 264, 270, 561, 567, 642
BmrFI CCNGG 5 cut(s) 202, 273, 491, 729, 846
BmsI GCATC 1 cut(s) 818
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 3 cut(s) 267, 630, 711
Bpu1102I GCTNAGC 1 cut(s) 759
BpuMI CCSGG 3 cut(s) 202, 273, 491
BsaAI YACGTR 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 5 cut(s) 159, 453, 489, 555, 844
BsaWI WCCGGW 2 cut(s) 224, 364
Bsc4I CCNNNNNNNGG 9 cut(s) 118, 122, 200, 231, 272, 292, 490, 611, 848
Bse118I RCCGGY 1 cut(s) 562
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 2 cut(s) 262, 706
BseAI TCCGGA 2 cut(s) 224, 364
BseBI CCWGG 2 cut(s) 729, 846
BseDI CCNNGG 5 cut(s) 159, 453, 489, 555, 844
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 9 cut(s) 118, 122, 200, 231, 272, 292, 490, 611, 848
BseMI GCAATG 2 cut(s) 262, 706
BseMII CTCAG 5 cut(s) 185, 380, 440, 627, 755
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 1 cut(s) 82
BseXI GCAGC 2 cut(s) 792, 870
BseYI CCCAGC 1 cut(s) 65
Bsh1236I CGCG 3 cut(s) 30, 242, 557
Bsh1285I CGRYCG 1 cut(s) 509
BshFI GGCC 2 cut(s) 5, 483
BshNI GGYRCC 1 cut(s) 565
BsiEI CGRYCG 1 cut(s) 509
BsiHKAI GWGCWC 1 cut(s) 336
BsiSI CCGG 7 cut(s) 201, 225, 266, 272, 365, 491, 563
BslFI GGGAC 2 cut(s) 47, 409
BslI CCNNNNNNNGG 9 cut(s) 118, 122, 200, 231, 272, 292, 490, 611, 848
BsmAI GTCTC 1 cut(s) 810
BsmFI GGGAC 2 cut(s) 47, 409
BsnI GGCC 2 cut(s) 5, 483
Bsp1286I GDGCHC 2 cut(s) 336, 344
Bsp13I TCCGGA 2 cut(s) 224, 364
Bsp143I GATC 5 cut(s) 126, 153, 303, 506, 731
Bsp1720I GCTNAGC 1 cut(s) 759
BspANI GGCC 2 cut(s) 5, 483
BspCNI CTCAG 5 cut(s) 184, 381, 439, 628, 756
BspEI TCCGGA 2 cut(s) 224, 364
BspFNI CGCG 3 cut(s) 30, 242, 557
BspLI GGNNCC 6 cut(s) 63, 264, 270, 561, 567, 642
BspPI GGATC 3 cut(s) 134, 311, 739
BspT107I GGYRCC 1 cut(s) 565
BsrBI CCGCTC 2 cut(s) 98, 105
BsrDI GCAATG 2 cut(s) 262, 706
BsrFI RCCGGY 1 cut(s) 562
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 562
BssECI CCNNGG 5 cut(s) 159, 453, 489, 555, 844
BssMI GATC 5 cut(s) 126, 153, 303, 506, 731
BssNI GRCGYC 1 cut(s) 566
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 2 cut(s) 729, 846
BstACI GRCGYC 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 3 cut(s) 291, 564, 839
BstDEI CTNAG 9 cut(s) 171, 345, 389, 426, 461, 636, 749, 759, 764
BstDSI CCRYGG 1 cut(s) 555
BstENI CCTNNNNNAGG 1 cut(s) 609
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 3 cut(s) 30, 242, 557
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 1 cut(s) 568
BstKTI GATC 5 cut(s) 129, 156, 306, 509, 734
BstMAI GTCTC 1 cut(s) 810
BstMBI GATC 5 cut(s) 126, 153, 303, 506, 731
BstMCI CGRYCG 1 cut(s) 509
BstMWI GCNNNNNNNGC 5 cut(s) 52, 95, 385, 563, 858
BstNI CCWGG 2 cut(s) 729, 846
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 5 cut(s) 200, 271, 489, 727, 844
BstUI CGCG 3 cut(s) 30, 242, 557
BstV1I GCAGC 2 cut(s) 792, 870
BstX2I RGATCY 1 cut(s) 731
BstYI RGATCY 1 cut(s) 731
BsuRI GGCC 2 cut(s) 5, 483
BtgI CCRYGG 1 cut(s) 555
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 2 cut(s) 18, 633
Cac8I GCNNGC 3 cut(s) 291, 564, 839
CaiI CAGNNNCTG 2 cut(s) 178, 734
CfoI GCGC 1 cut(s) 568
Cfr10I RCCGGY 1 cut(s) 562
Cfr13I GGNCC 3 cut(s) 61, 482, 640
Cfr42I CCGCGG 1 cut(s) 558
Csp6I GTAC 1 cut(s) 205
CviAII CATG 1 cut(s) 43
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 9 cut(s) 171, 345, 389, 426, 461, 636, 749, 759, 764
DinI GGCGCC 1 cut(s) 567
DpnI GATC 5 cut(s) 128, 155, 305, 508, 733
DpnII GATC 5 cut(s) 126, 153, 303, 506, 731
DrdI GACNNNNNNGTC 2 cut(s) 403, 647
DseDI GACNNNNNNGTC 2 cut(s) 403, 647
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 61, 640
Eco72I CACGTG 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 609
EcoO109I RGGNCCY 2 cut(s) 61, 640
EcoRII CCWGG 2 cut(s) 727, 844
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 344
EgeI GGCGCC 1 cut(s) 567
EheI GGCGCC 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 1 cut(s) 46
FaiI YATR 5 cut(s) 44, 537, 672, 707, 863
FaqI GGGAC 2 cut(s) 47, 409
FatI CATG 1 cut(s) 42
FauI CCCGC 2 cut(s) 88, 224
Fnu4HI GCNGC 5 cut(s) 28, 243, 781, 807, 859
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 5 cut(s) 28, 243, 781, 807, 859
FspBI CTAG 1 cut(s) 824
GlaI GCGC 1 cut(s) 567
GluI GCNGC 5 cut(s) 28, 243, 781, 807, 859
GsaI CCCAGC 1 cut(s) 69
GsuI CTGGAG 3 cut(s) 267, 630, 711
HaeII RGCGCY 1 cut(s) 569
HaeIII GGCC 2 cut(s) 5, 483
HapII CCGG 7 cut(s) 201, 225, 266, 272, 365, 491, 563
HhaI GCGC 1 cut(s) 568
Hin1I GRCGYC 1 cut(s) 566
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 566
HinP1I GCGC 1 cut(s) 566
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 4 cut(s) 74, 416, 424, 551
HpaII CCGG 7 cut(s) 201, 225, 266, 272, 365, 491, 563
HphI GGTGA 4 cut(s) 92, 101, 148, 307
Hpy166II GTNNAC 4 cut(s) 283, 328, 523, 633
Hpy188I TCNGA 7 cut(s) 73, 121, 153, 174, 409, 429, 618
Hpy188III TCNNGA 4 cut(s) 225, 365, 652, 813
Hpy8I GTNNAC 4 cut(s) 283, 328, 523, 633
Hpy99I CGWCG 2 cut(s) 121, 358
HpyAV CCTTC 3 cut(s) 208, 245, 424
HpyCH4IV ACGT 2 cut(s) 50, 336
HpyCH4V TGCA 2 cut(s) 403, 686
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 95, 385, 563, 858
HpyF3I CTNAG 9 cut(s) 171, 345, 389, 426, 461, 636, 749, 759, 764
HpySE526I ACGT 2 cut(s) 50, 336
Hsp92I GRCGYC 1 cut(s) 566
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 566
KasI GGCGCC 1 cut(s) 565
Kpn2I TCCGGA 2 cut(s) 224, 364
KroI GCCGGC 1 cut(s) 562
KroNI GCCGGC 1 cut(s) 564
KspI CCGCGG 1 cut(s) 558
Kzo9I GATC 5 cut(s) 126, 153, 303, 506, 731
LmnI GCTCC 7 cut(s) 74, 203, 262, 268, 385, 457, 559
Lsp1109I GCAGC 2 cut(s) 792, 870
LweI GCATC 1 cut(s) 818
MaeI CTAG 1 cut(s) 824
MaeII ACGT 2 cut(s) 50, 336
MalI GATC 5 cut(s) 128, 155, 305, 508, 733
MbiI CCGCTC 2 cut(s) 98, 105
MboI GATC 5 cut(s) 126, 153, 303, 506, 731
MboII GAAGA 2 cut(s) 176, 199
MflI RGATCY 1 cut(s) 731
MhlI GDGCHC 2 cut(s) 336, 344
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 410, 495, 666
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 566
MlyI GAGTC 2 cut(s) 68, 418
MmeI TCCRAC 3 cut(s) 96, 99, 387
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 224, 364
MroNI GCCGGC 1 cut(s) 562
MroXI GAANNNNTTC 1 cut(s) 498
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 513, 711, 874
MslI CAYNNNNRTG 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 557
MspI CCGG 7 cut(s) 201, 225, 266, 272, 365, 491, 563
MspR9I CCNGG 5 cut(s) 202, 273, 491, 729, 846
MvaI CCWGG 2 cut(s) 729, 846
MvnI CGCG 3 cut(s) 30, 242, 557
MwoI GCNNNNNNNGC 5 cut(s) 52, 95, 385, 563, 858
NaeI GCCGGC 1 cut(s) 564
NarI GGCGCC 1 cut(s) 566
NciI CCSGG 3 cut(s) 202, 273, 491
NdeII GATC 5 cut(s) 126, 153, 303, 506, 731
NgoMIV GCCGGC 1 cut(s) 562
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 6 cut(s) 63, 264, 270, 561, 567, 642
NspI RCATGY 1 cut(s) 46
OliI CACNNNNGTG 1 cut(s) 11
PdiI GCCGGC 1 cut(s) 564
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 2 cut(s) 416, 551
PfoI TCCNGGA 1 cut(s) 727
PkrI GCNGC 5 cut(s) 29, 244, 782, 808, 860
Ple19I CGATCG 1 cut(s) 509
PleI GAGTC 2 cut(s) 68, 418
PluTI GGCGCC 1 cut(s) 569
PmaCI CACGTG 1 cut(s) 337
PmlI CACGTG 1 cut(s) 337
PpsI GAGTC 2 cut(s) 68, 418
Ppu21I YACGTR 1 cut(s) 337
PpuMI RGGWCCY 2 cut(s) 61, 640
Psp5II RGGWCCY 2 cut(s) 61, 640
Psp6I CCWGG 2 cut(s) 727, 844
PspCI CACGTG 1 cut(s) 337
PspFI CCCAGC 1 cut(s) 65
PspGI CCWGG 2 cut(s) 727, 844
PspN4I GGNNCC 6 cut(s) 63, 264, 270, 561, 567, 642
PspPI GGNCC 3 cut(s) 61, 482, 640
PspPPI RGGWCCY 2 cut(s) 61, 640
PstNI CAGNNNCTG 2 cut(s) 178, 734
PsuI RGATCY 1 cut(s) 731
PvuI CGATCG 1 cut(s) 509
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 11
SacII CCGCGG 1 cut(s) 558
SaqAI TTAA 3 cut(s) 513, 711, 874
SatI GCNGC 5 cut(s) 28, 243, 781, 807, 859
Sau3AI GATC 5 cut(s) 126, 153, 303, 506, 731
Sau96I GGNCC 3 cut(s) 61, 482, 640
SchI GAGTC 2 cut(s) 68, 418
ScrFI CCNGG 5 cut(s) 202, 273, 491, 729, 846
SduI GDGCHC 2 cut(s) 336, 344
SfaNI GCATC 1 cut(s) 818
SfoI GGCGCC 1 cut(s) 567
Sfr303I CCGCGG 1 cut(s) 558
SgrBI CCGCGG 1 cut(s) 558
SinI GGWCC 2 cut(s) 61, 640
SmiMI CAYNNNNRTG 1 cut(s) 11
Sse9I AATT 3 cut(s) 410, 495, 666
SspDI GGCGCC 1 cut(s) 565
SspMI CTAG 1 cut(s) 824
StyD4I CCNGG 5 cut(s) 200, 271, 489, 727, 844
StyI CCWWGG 1 cut(s) 453
TaiI ACGT 2 cut(s) 53, 339
TasI AATT 3 cut(s) 410, 495, 666
TauI GCSGC 3 cut(s) 30, 245, 809
TfiI GAWTC 2 cut(s) 416, 551
Tru1I TTAA 3 cut(s) 513, 711, 874
Tru9I TTAA 3 cut(s) 513, 711, 874
TscAI CASTG 2 cut(s) 18, 640
TseI GCWGC 2 cut(s) 780, 858
TspDTI ATGAA 2 cut(s) 227, 659
TspRI CASTG 2 cut(s) 18, 640
VpaK11BI GGWCC 2 cut(s) 61, 640
XagI CCTNNNNNAGG 1 cut(s) 609
XapI RAATTY 1 cut(s) 666
XceI RCATGY 1 cut(s) 46
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.