Rroxscaffold_2G00130150

Protein of unknown function (DUF4057)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009044
Physical Location & Seq
Reverse (-)
66270891 .. 66272775
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130150.1

Sequence Viewer

Length: 906 bp
ATGGCCACACCAGTGAGAAGGCCTCGCCGCGACACCGCCGACATGCTGACGTGGCAAGAGGTCCCTCGCTCCGACTCTCCCGCCACCGCCTCCTCCGCCCACCGCTCTCACCAGCCGTCGGATGGGATCAGCAAGGTGCTCCACGGCGGTCAGATCACCGAGGAAGAAGCTCAGAGCCTGAACAAGAAGAAGCCTTGCTCCGGGTACAAAATGAAGGAGATTTCCGGAAGCGGGATATTCGCGGCTGGAGAAGGCAATGCGGAGCCGGAGCCGGGTCGGGTTTACCAGCAGGCGGTGAAAGGGATCAGTCAAATTTCGTTCAGCGTTGAGGAGCACGTGAGCCCGAAGAAGCCGACGAGTGTTCCGGAGGTGGCGAAGCAGAAGGAGCTGAGTGGGACTTTGCAGTCGGAATTGGATTCCAAGACTCAGAAGGGGATTTCAAATGCCAAGACCAAGGAGCTTAGTGGAAATGACATCTTTGGCCCTCCCCGGAAATTGTTCCCCGATCGGTTAATGCTGTTCACACCCACGAGTATAAGCAGAGTAAAGATTCCGCGGAGCCGGCGCCCAGAAATCTCCGCACCTCTGTCAAAGTTTCCAATATCAATTGGGATTCGACTGCTTCAGATTAATCCTGCTGGAGGTCAAAGTAAAATCTTGTTCACTGAGGACCCTGTTGTCAAGACTTCCAAGAAAATTCATAACCAGAAGTTTGCAGAGCTGACAGGCAATGACATATTTAAGGGAGATGTTTCGCCGGGATCTGCTGAAAAGCCACTAAGCACGGCTAAGCTGAGAGAAATGAGTGGCAGCAACATCTTTGCTGATGGAAAGGCGGCATCAAGAGACTATCTAGGTGGTGTCCGCAAGCCCCCTGGTGGCGAGAGCAGCATATCATTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4057

Protein Analysis

301

Amino Acids

32.45

Weight (kDa)

9.74

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4057 PF13266 2 - 299 1.6e-126 Protein of unknown function (DUF4057)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 403, 677
AccB1I GGYRCC 1 cut(s) 564
AccBSI CCGCTC 1 cut(s) 105
AccII CGCG 3 cut(s) 30, 242, 556
AccIII TCCGGA 2 cut(s) 224, 364
AclWI GGATC 3 cut(s) 134, 311, 769
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 312, 696
AcuI CTGAAG 1 cut(s) 608
AcvI CACGTG 1 cut(s) 337
AcyI GRCGYC 1 cut(s) 565
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 8 cut(s) 118, 122, 200, 231, 272, 292, 641, 878
AgsI TTSAA 1 cut(s) 441
AjiI CACGTC 1 cut(s) 51
AjnI CCWGG 1 cut(s) 874
AleI CACNNNNGTG 1 cut(s) 11
AluBI AGCT 5 cut(s) 170, 388, 460, 721, 793
AluI AGCT 5 cut(s) 170, 388, 460, 721, 793
Alw21I GWGCWC 2 cut(s) 141, 336
Alw26I GTCTC 1 cut(s) 840
AlwI GGATC 3 cut(s) 134, 311, 769
AlwNI CAGNNNCTG 1 cut(s) 178
Aor13HI TCCGGA 2 cut(s) 224, 364
AoxI GGCC 3 cut(s) 3, 20, 481
ApeKI GCWGC 2 cut(s) 810, 888
ApoI RAATTY 2 cut(s) 312, 696
AseI ATTAAT 1 cut(s) 630
Asp700I GAANNNNTTC 1 cut(s) 497
AspLEI GCGC 1 cut(s) 567
AspS9I GGNCC 3 cut(s) 61, 482, 670
AsuC2I CCSGG 4 cut(s) 202, 273, 490, 759
AsuHPI GGTGA 3 cut(s) 101, 148, 307
AvaII GGWCC 2 cut(s) 61, 670
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 564
BanII GRGCYC 1 cut(s) 344
BauI CACGAG 1 cut(s) 529
BbrPI CACGTG 1 cut(s) 337
Bbv12I GWGCWC 2 cut(s) 141, 336
BbvI GCAGC 2 cut(s) 822, 900
BccI CCATC 2 cut(s) 116, 821
BceAI ACGGC 3 cut(s) 100, 160, 801
BciT130I CCWGG 1 cut(s) 876
BcnI CCSGG 4 cut(s) 202, 273, 490, 759
BcoDI GTCTC 1 cut(s) 840
BfaI CTAG 1 cut(s) 854
BfoI RGCGCY 1 cut(s) 568
BisI GCNGC 5 cut(s) 28, 243, 811, 837, 889
BlpI GCTNAGC 1 cut(s) 789
BlsI GCNGC 5 cut(s) 29, 244, 812, 838, 890
Bme1390I CCNGG 5 cut(s) 202, 273, 490, 759, 876
Bme18I GGWCC 2 cut(s) 61, 670
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 3 cut(s) 61, 482, 670
BmiI GGNNCC 6 cut(s) 63, 264, 270, 560, 566, 672
BmrFI CCNGG 5 cut(s) 202, 273, 490, 759, 876
BmsI GCATC 1 cut(s) 848
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 2 cut(s) 267, 660
Bpu1102I GCTNAGC 1 cut(s) 789
BpuMI CCSGG 4 cut(s) 202, 273, 490, 759
BsaAI YACGTR 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 565
BsaJI CCNNGG 6 cut(s) 142, 159, 453, 488, 554, 874
BsaWI WCCGGW 2 cut(s) 224, 364
Bsc4I CCNNNNNNNGG 8 cut(s) 118, 122, 200, 231, 272, 292, 641, 878
Bse118I RCCGGY 1 cut(s) 561
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 2 cut(s) 262, 736
BseAI TCCGGA 2 cut(s) 224, 364
BseBI CCWGG 1 cut(s) 876
BseDI CCNNGG 6 cut(s) 142, 159, 453, 488, 554, 874
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 8 cut(s) 118, 122, 200, 231, 272, 292, 641, 878
BseMI GCAATG 2 cut(s) 262, 736
BseMII CTCAG 5 cut(s) 185, 380, 440, 657, 785
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 2 cut(s) 82, 344
BseXI GCAGC 2 cut(s) 822, 900
Bsh1236I CGCG 3 cut(s) 30, 242, 556
Bsh1285I CGRYCG 1 cut(s) 508
BshFI GGCC 3 cut(s) 5, 22, 483
BshNI GGYRCC 1 cut(s) 564
BsiEI CGRYCG 1 cut(s) 508
BsiHKAI GWGCWC 2 cut(s) 141, 336
BsiSI CCGG 8 cut(s) 201, 225, 266, 272, 365, 490, 562, 758
BslFI GGGAC 2 cut(s) 47, 409
BslI CCNNNNNNNGG 8 cut(s) 118, 122, 200, 231, 272, 292, 641, 878
BsmAI GTCTC 1 cut(s) 840
BsmFI GGGAC 2 cut(s) 47, 409
BsnI GGCC 3 cut(s) 5, 22, 483
Bsp1286I GDGCHC 3 cut(s) 141, 336, 344
Bsp13I TCCGGA 2 cut(s) 224, 364
Bsp143I GATC 5 cut(s) 126, 153, 303, 505, 761
Bsp1720I GCTNAGC 1 cut(s) 789
BspANI GGCC 3 cut(s) 5, 22, 483
BspCNI CTCAG 5 cut(s) 184, 381, 439, 658, 786
BspEI TCCGGA 2 cut(s) 224, 364
BspFNI CGCG 3 cut(s) 30, 242, 556
BspLI GGNNCC 6 cut(s) 63, 264, 270, 560, 566, 672
BspPI GGATC 3 cut(s) 134, 311, 769
BspT107I GGYRCC 1 cut(s) 564
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 2 cut(s) 262, 736
BsrFI RCCGGY 1 cut(s) 561
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 561
BssECI CCNNGG 6 cut(s) 142, 159, 453, 488, 554, 874
BssMI GATC 5 cut(s) 126, 153, 303, 505, 761
BssNI GRCGYC 1 cut(s) 565
BssSI CACGAG 1 cut(s) 529
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 529
Bst2UI CCWGG 1 cut(s) 876
BstACI GRCGYC 1 cut(s) 565
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 3 cut(s) 291, 563, 869
BstDEI CTNAG 8 cut(s) 171, 389, 426, 461, 666, 779, 789, 794
BstDSI CCRYGG 2 cut(s) 142, 554
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 3 cut(s) 30, 242, 556
BstH2I RGCGCY 1 cut(s) 568
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 5 cut(s) 129, 156, 306, 508, 764
BstMAI GTCTC 1 cut(s) 840
BstMBI GATC 5 cut(s) 126, 153, 303, 505, 761
BstMCI CGRYCG 1 cut(s) 508
BstMWI GCNNNNNNNGC 5 cut(s) 52, 95, 385, 562, 888
BstNI CCWGG 1 cut(s) 876
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 5 cut(s) 200, 271, 488, 757, 874
BstUI CGCG 3 cut(s) 30, 242, 556
BstV1I GCAGC 2 cut(s) 822, 900
BstX2I RGATCY 1 cut(s) 761
BstYI RGATCY 1 cut(s) 761
BsuRI GGCC 3 cut(s) 5, 22, 483
BtgI CCRYGG 2 cut(s) 142, 554
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 2 cut(s) 18, 663
Cac8I GCNNGC 3 cut(s) 291, 563, 869
CaiI CAGNNNCTG 1 cut(s) 178
CfoI GCGC 1 cut(s) 567
Cfr10I RCCGGY 1 cut(s) 561
Cfr13I GGNCC 3 cut(s) 61, 482, 670
Cfr42I CCGCGG 1 cut(s) 557
Csp6I GTAC 1 cut(s) 205
CviAII CATG 1 cut(s) 43
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 8 cut(s) 171, 389, 426, 461, 666, 779, 789, 794
DinI GGCGCC 1 cut(s) 566
DpnI GATC 5 cut(s) 128, 155, 305, 507, 763
DpnII GATC 5 cut(s) 126, 153, 303, 505, 761
DrdI GACNNNNNNGTC 2 cut(s) 403, 677
DseDI GACNNNNNNGTC 2 cut(s) 403, 677
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 61, 670
Eco57I CTGAAG 1 cut(s) 608
Eco72I CACGTG 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoO109I RGGNCCY 2 cut(s) 61, 670
EcoRII CCWGG 1 cut(s) 874
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 344
EgeI GGCGCC 1 cut(s) 566
EheI GGCGCC 1 cut(s) 566
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 1 cut(s) 46
FaiI YATR 5 cut(s) 44, 536, 702, 737, 893
FaqI GGGAC 2 cut(s) 47, 409
FatI CATG 1 cut(s) 42
FauI CCCGC 2 cut(s) 88, 224
Fnu4HI GCNGC 5 cut(s) 28, 243, 811, 837, 889
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 5 cut(s) 28, 243, 811, 837, 889
FspBI CTAG 1 cut(s) 854
GlaI GCGC 1 cut(s) 566
GluI GCNGC 5 cut(s) 28, 243, 811, 837, 889
GsuI CTGGAG 2 cut(s) 267, 660
HaeII RGCGCY 1 cut(s) 568
HaeIII GGCC 3 cut(s) 5, 22, 483
HapII CCGG 8 cut(s) 201, 225, 266, 272, 365, 490, 562, 758
HhaI GCGC 1 cut(s) 567
Hin1I GRCGYC 1 cut(s) 565
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HinfI GANTC 5 cut(s) 74, 416, 424, 550, 613
HpaII CCGG 8 cut(s) 201, 225, 266, 272, 365, 490, 562, 758
HphI GGTGA 3 cut(s) 101, 148, 307
Hpy166II GTNNAC 3 cut(s) 283, 522, 663
Hpy188I TCNGA 7 cut(s) 73, 121, 153, 174, 409, 429, 627
Hpy188III TCNNGA 4 cut(s) 225, 365, 682, 843
Hpy8I GTNNAC 3 cut(s) 283, 522, 663
Hpy99I CGWCG 2 cut(s) 121, 358
HpyAV CCTTC 5 cut(s) 12, 208, 245, 376, 424
HpyCH4IV ACGT 2 cut(s) 50, 336
HpyCH4V TGCA 2 cut(s) 403, 716
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 95, 385, 562, 888
HpyF3I CTNAG 8 cut(s) 171, 389, 426, 461, 666, 779, 789, 794
HpySE526I ACGT 2 cut(s) 50, 336
Hsp92I GRCGYC 1 cut(s) 565
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 565
KasI GGCGCC 1 cut(s) 564
Kpn2I TCCGGA 2 cut(s) 224, 364
KroI GCCGGC 1 cut(s) 561
KroNI GCCGGC 1 cut(s) 563
KspI CCGCGG 1 cut(s) 557
Kzo9I GATC 5 cut(s) 126, 153, 303, 505, 761
LmnI GCTCC 9 cut(s) 74, 144, 203, 262, 268, 331, 385, 457, 558
Lsp1109I GCAGC 2 cut(s) 822, 900
LweI GCATC 1 cut(s) 848
MaeI CTAG 1 cut(s) 854
MaeII ACGT 2 cut(s) 50, 336
MalI GATC 5 cut(s) 128, 155, 305, 507, 763
MbiI CCGCTC 1 cut(s) 105
MboI GATC 5 cut(s) 126, 153, 303, 505, 761
MboII GAAGA 3 cut(s) 176, 199, 358
MfeI CAATTG 1 cut(s) 606
MflI RGATCY 1 cut(s) 761
MhlI GDGCHC 3 cut(s) 141, 336, 344
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 312, 410, 494, 606, 696
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 565
MlyI GAGTC 2 cut(s) 68, 418
MmeI TCCRAC 3 cut(s) 96, 99, 387
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 224, 364
MroNI GCCGGC 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 497
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 512, 630, 741, 904
MslI CAYNNNNRTG 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 556
MspI CCGG 8 cut(s) 201, 225, 266, 272, 365, 490, 562, 758
MspR9I CCNGG 5 cut(s) 202, 273, 490, 759, 876
MunI CAATTG 1 cut(s) 606
MvaI CCWGG 1 cut(s) 876
MvnI CGCG 3 cut(s) 30, 242, 556
MwoI GCNNNNNNNGC 5 cut(s) 52, 95, 385, 562, 888
NaeI GCCGGC 1 cut(s) 563
NarI GGCGCC 1 cut(s) 565
NciI CCSGG 4 cut(s) 202, 273, 490, 759
NdeII GATC 5 cut(s) 126, 153, 303, 505, 761
NgoMIV GCCGGC 1 cut(s) 561
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 6 cut(s) 63, 264, 270, 560, 566, 672
NspI RCATGY 1 cut(s) 46
OliI CACNNNNGTG 1 cut(s) 11
PceI AGGCCT 1 cut(s) 22
PdiI GCCGGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 3 cut(s) 416, 550, 613
PkrI GCNGC 5 cut(s) 29, 244, 812, 838, 890
Ple19I CGATCG 1 cut(s) 508
PleI GAGTC 2 cut(s) 68, 418
PluTI GGCGCC 1 cut(s) 568
PmaCI CACGTG 1 cut(s) 337
PmlI CACGTG 1 cut(s) 337
PpsI GAGTC 2 cut(s) 68, 418
Ppu21I YACGTR 1 cut(s) 337
PpuMI RGGWCCY 2 cut(s) 61, 670
PshBI ATTAAT 1 cut(s) 630
Psp5II RGGWCCY 2 cut(s) 61, 670
Psp6I CCWGG 1 cut(s) 874
PspCI CACGTG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 6 cut(s) 63, 264, 270, 560, 566, 672
PspPI GGNCC 3 cut(s) 61, 482, 670
PspPPI RGGWCCY 2 cut(s) 61, 670
PstNI CAGNNNCTG 1 cut(s) 178
PsuI RGATCY 1 cut(s) 761
PvuI CGATCG 1 cut(s) 508
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 11
SacII CCGCGG 1 cut(s) 557
SaqAI TTAA 4 cut(s) 512, 630, 741, 904
SatI GCNGC 5 cut(s) 28, 243, 811, 837, 889
Sau3AI GATC 5 cut(s) 126, 153, 303, 505, 761
Sau96I GGNCC 3 cut(s) 61, 482, 670
SchI GAGTC 2 cut(s) 68, 418
ScrFI CCNGG 5 cut(s) 202, 273, 490, 759, 876
SduI GDGCHC 3 cut(s) 141, 336, 344
SfaNI GCATC 1 cut(s) 848
SfoI GGCGCC 1 cut(s) 566
Sfr303I CCGCGG 1 cut(s) 557
SgrBI CCGCGG 1 cut(s) 557
SinI GGWCC 2 cut(s) 61, 670
SmiMI CAYNNNNRTG 1 cut(s) 11
Sse9I AATT 5 cut(s) 312, 410, 494, 606, 696
SseBI AGGCCT 1 cut(s) 22
SspDI GGCGCC 1 cut(s) 564
SspMI CTAG 1 cut(s) 854
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 5 cut(s) 200, 271, 488, 757, 874
StyI CCWWGG 1 cut(s) 453
TaiI ACGT 2 cut(s) 53, 339
TaqI TCGA 1 cut(s) 616
TasI AATT 5 cut(s) 312, 410, 494, 606, 696
TauI GCSGC 3 cut(s) 30, 245, 839
TfiI GAWTC 3 cut(s) 416, 550, 613
Tru1I TTAA 4 cut(s) 512, 630, 741, 904
Tru9I TTAA 4 cut(s) 512, 630, 741, 904
TscAI CASTG 2 cut(s) 18, 670
TseI GCWGC 2 cut(s) 810, 888
TspDTI ATGAA 2 cut(s) 227, 689
TspRI CASTG 2 cut(s) 18, 670
VpaK11BI GGWCC 2 cut(s) 61, 670
VspI ATTAAT 1 cut(s) 630
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 2 cut(s) 312, 696
XceI RCATGY 1 cut(s) 46
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 1 cut(s) 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.