MD15G1324500.v1.1

Protein of unknown function (DUF4057)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009044
Physical Location & Seq
Reverse (-)
34549540 .. 34551872
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1324500.v1.1.491

Sequence Viewer

Length: 906 bp
ATGGAGAGAACACCACTGAGAAAGCCTCGCAGAGACACTGCCGATATGCTGACGTGGAAAGAGGTCCCACACTCCGACTCTCCGCCCACCGCCTCCTCCGCCCACCGCTCTCATCAGCCGTCGGATGGAATCAGCAAGGTGCTCCATGGCGGTCAGATCACTGATGAAGAAGCTCAGAGCCTCAACAAACAGAAGCCTTGTTCAGGCTATAAACTGAAGGAGATTACTGGAAATGGCATATTTGCAGCGGGGTCAGGCAAGGATGCTGCACCAGAATCTAATAATGCTAGTAACAAAACGGGGCTTCGTATTTATCAGCAAGCACTCAATGGAGTCAGCCAAATCTCATTCAGTGTTGATGAACATGTCACGCCTAAGAAGCCTGTCACTGTTCCTGAGGTTGCCAAGCAGCGTGAATTGAGTGGGACATTGCAGTCCAACTCCGATTTAAAATCCAAGAAGAGCGGATCAAATGCCAAGTCAAAGGAGCTCACTGGAAATGACATCTTTGGCCCTCCTCCTGAAGTCGTGCCTCGATCATTGGCTGCTGCTCACACCCTGCAAACTAGAGAAACCAGAGACATTGGGGAGCCTGCACCCCGAAATCTACGCACCTCTGTCAAAGTTTCAAATCCTGCTGGAGGTCAGAGTAATATTATGTTCAGTGAGGAACCTGTTTCCAAGACTTCTAGGAAAATACATAACCAGAAGTTTGCAGAGCTGACAGGGAATGATATATTTAAAGGTGATGTTGCTCCAGGGTCTACTGAAAAGCCCCTCAGCACGGCTAAGCTGAGAGAGATGAATGGCAGCAACATCTTTGCTGATGGAAAGGCATCATCCAGAGACTATCTGGGTGGTGTTCGCAAGCCCCCCGGTGGAGAGAGCAGCATTGCCTTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4057

Protein Analysis

302

Amino Acids

32.19

Weight (kDa)

9.52

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4057 PF13266 3 - 299 1.9e-146 Protein of unknown function (DUF4057)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 433
AccBSI CCGCTC 2 cut(s) 108, 465
AccI GTMKAC 1 cut(s) 764
AciI CCGC 7 cut(s) 83, 90, 99, 106, 150, 248, 465
AclWI GGATC 1 cut(s) 475
AcuI CTGAAG 2 cut(s) 236, 543
AfiI CCNNNNNNNGG 5 cut(s) 125, 203, 641, 784, 878
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 1 cut(s) 630
AjiI CACGTC 1 cut(s) 54
AjnI CCWGG 1 cut(s) 757
AluBI AGCT 4 cut(s) 173, 490, 721, 793
AluI AGCT 4 cut(s) 173, 490, 721, 793
Alw21I GWGCWC 2 cut(s) 144, 492
Alw26I GTCTC 3 cut(s) 27, 573, 840
AlwI GGATC 1 cut(s) 475
AoxI GGCC 1 cut(s) 511
ApeKI GCWGC 7 cut(s) 245, 266, 409, 545, 548, 810, 888
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AspS9I GGNCC 2 cut(s) 64, 512
AsuC2I CCSGG 1 cut(s) 876
AsuHPI GGTGA 1 cut(s) 758
AvaII GGWCC 1 cut(s) 64
AxyI CCTNAGG 1 cut(s) 396
BanII GRGCYC 1 cut(s) 492
Bbv12I GWGCWC 2 cut(s) 144, 492
BbvCI CCTCAGC 1 cut(s) 779
BbvI GCAGC 7 cut(s) 253, 257, 421, 532, 535, 822, 900
BccI CCATC 2 cut(s) 119, 821
BceAI ACGGC 2 cut(s) 103, 801
BciT130I CCWGG 1 cut(s) 759
BcnI CCSGG 1 cut(s) 876
BcoDI GTCTC 3 cut(s) 27, 573, 840
BfaI CTAG 3 cut(s) 288, 567, 690
BisI GCNGC 7 cut(s) 246, 267, 410, 546, 549, 811, 889
BlpI GCTNAGC 1 cut(s) 789
BlsI GCNGC 7 cut(s) 247, 268, 411, 547, 550, 812, 890
Bme1390I CCNGG 2 cut(s) 759, 876
Bme18I GGWCC 1 cut(s) 64
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 2 cut(s) 64, 512
BmiI GGNNCC 3 cut(s) 66, 591, 672
BmrFI CCNGG 2 cut(s) 759, 876
BmsI GCATC 2 cut(s) 253, 845
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 2 cut(s) 660, 741
Bpu10I CCTNAGC 1 cut(s) 779
Bpu1102I GCTNAGC 1 cut(s) 789
BpuMI CCSGG 1 cut(s) 876
BsaJI CCNNGG 4 cut(s) 145, 758, 874, 897
Bsc4I CCNNNNNNNGG 5 cut(s) 125, 203, 641, 784, 878
Bse1I ACTGG 2 cut(s) 232, 499
Bse21I CCTNAGG 1 cut(s) 396
Bse3DI GCAATG 2 cut(s) 428, 891
BseBI CCWGG 1 cut(s) 759
BseDI CCNNGG 4 cut(s) 145, 758, 874, 897
BseGI GGATG 3 cut(s) 130, 268, 839
BseLI CCNNNNNNNGG 5 cut(s) 125, 203, 641, 784, 878
BseMI GCAATG 2 cut(s) 428, 891
BseMII CTCAG 5 cut(s) 8, 188, 387, 785, 793
BseNI ACTGG 2 cut(s) 232, 499
BseRI GAGGAG 2 cut(s) 85, 507
BseXI GCAGC 7 cut(s) 253, 257, 421, 532, 535, 822, 900
BsgI GTGCAG 2 cut(s) 252, 579
BshFI GGCC 1 cut(s) 513
BsiHKAI GWGCWC 2 cut(s) 144, 492
BsiSI CCGG 1 cut(s) 876
BslFI GGGAC 2 cut(s) 50, 439
BslI CCNNNNNNNGG 5 cut(s) 125, 203, 641, 784, 878
BsmAI GTCTC 3 cut(s) 27, 573, 840
BsmFI GGGAC 2 cut(s) 50, 439
BsnI GGCC 1 cut(s) 513
Bsp1286I GDGCHC 2 cut(s) 144, 492
Bsp143I GATC 3 cut(s) 156, 467, 536
Bsp1720I GCTNAGC 1 cut(s) 789
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 7 cut(s) 83, 90, 99, 106, 150, 248, 465
BspANI GGCC 1 cut(s) 513
BspCNI CTCAG 5 cut(s) 9, 187, 388, 786, 792
BspLI GGNNCC 3 cut(s) 66, 591, 672
BspPI GGATC 1 cut(s) 475
BspQI GCTCTTC 1 cut(s) 455
BsrBI CCGCTC 2 cut(s) 108, 465
BsrDI GCAATG 2 cut(s) 428, 891
BsrI ACTGG 2 cut(s) 232, 499
BssECI CCNNGG 4 cut(s) 145, 758, 874, 897
BssMI GATC 3 cut(s) 156, 467, 536
BssT1I CCWWGG 2 cut(s) 145, 897
Bst2UI CCWGG 1 cut(s) 759
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 1 cut(s) 455
BstC8I GCNNGC 3 cut(s) 321, 594, 869
BstDEI CTNAG 7 cut(s) 17, 174, 375, 396, 779, 789, 794
BstDSI CCRYGG 1 cut(s) 145
BstENI CCTNNNNNAGG 2 cut(s) 201, 639
BstF5I GGATG 3 cut(s) 130, 268, 839
BstKTI GATC 3 cut(s) 159, 470, 539
BstMAI GTCTC 3 cut(s) 27, 573, 840
BstMBI GATC 3 cut(s) 156, 467, 536
BstMWI GCNNNNNNNGC 2 cut(s) 98, 379
BstNI CCWGG 1 cut(s) 759
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 757, 874
BstV1I GCAGC 7 cut(s) 253, 257, 421, 532, 535, 822, 900
Bsu36I CCTNAGG 1 cut(s) 396
BsuRI GGCC 1 cut(s) 513
BtgI CCRYGG 1 cut(s) 145
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 3 cut(s) 130, 268, 839
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 7 cut(s) 14, 36, 159, 358, 387, 492, 670
Cac8I GCNNGC 3 cut(s) 321, 594, 869
Cfr13I GGNCC 2 cut(s) 64, 512
CviAII CATG 2 cut(s) 146, 365
DdeI CTNAG 7 cut(s) 17, 174, 375, 396, 779, 789, 794
DpnI GATC 3 cut(s) 158, 469, 538
DpnII GATC 3 cut(s) 156, 467, 536
DraI TTTAAA 2 cut(s) 450, 742
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
EciI GGCGGA 2 cut(s) 72, 88
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 2 cut(s) 145, 897
Eco24I GRGCYC 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 490
Eco57I CTGAAG 2 cut(s) 236, 543
Eco81I CCTNAGG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 490
EcoNI CCTNNNNNAGG 2 cut(s) 201, 639
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 757
EcoT14I CCWWGG 2 cut(s) 145, 897
EcoT38I GRGCYC 1 cut(s) 492
ErhI CCWWGG 2 cut(s) 145, 897
FaeI CATG 2 cut(s) 149, 368
FaiI YATR 8 cut(s) 47, 147, 210, 239, 366, 659, 702, 737
FaqI GGGAC 2 cut(s) 50, 439
FatI CATG 2 cut(s) 145, 364
FauI CCCGC 1 cut(s) 241
FblI GTMKAC 1 cut(s) 764
Fnu4HI GCNGC 7 cut(s) 246, 267, 410, 546, 549, 811, 889
FokI GGATG 3 cut(s) 137, 275, 826
FriOI GRGCYC 1 cut(s) 492
Fsp4HI GCNGC 7 cut(s) 246, 267, 410, 546, 549, 811, 889
FspBI CTAG 3 cut(s) 288, 567, 690
GluI GCNGC 7 cut(s) 246, 267, 410, 546, 549, 811, 889
GsuI CTGGAG 2 cut(s) 660, 741
HaeIII GGCC 1 cut(s) 513
HapII CCGG 1 cut(s) 876
Hin1II CATG 2 cut(s) 149, 368
HinfI GANTC 4 cut(s) 77, 129, 275, 333
HpaII CCGG 1 cut(s) 876
HphI GGTGA 1 cut(s) 758
Hpy166II GTNNAC 1 cut(s) 765
Hpy188I TCNGA 6 cut(s) 76, 124, 156, 177, 445, 648
Hpy188III TCNNGA 3 cut(s) 395, 521, 843
Hpy8I GTNNAC 1 cut(s) 765
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 6 cut(s) 245, 269, 433, 562, 596, 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 379
HpyF3I CTNAG 7 cut(s) 17, 174, 375, 396, 779, 789, 794
HpySE526I ACGT 1 cut(s) 53
Hsp92II CATG 2 cut(s) 149, 368
Kzo9I GATC 3 cut(s) 156, 467, 536
LguI GCTCTTC 1 cut(s) 455
LmnI GCTCC 4 cut(s) 147, 487, 589, 760
Lsp1109I GCAGC 7 cut(s) 253, 257, 421, 532, 535, 822, 900
LweI GCATC 2 cut(s) 253, 845
MaeI CTAG 3 cut(s) 288, 567, 690
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 3 cut(s) 290, 367, 385
MalI GATC 3 cut(s) 158, 469, 538
MbiI CCGCTC 2 cut(s) 108, 465
MboI GATC 3 cut(s) 156, 467, 536
MboII GAAGA 2 cut(s) 179, 472
MhlI GDGCHC 2 cut(s) 144, 492
MluCI AATT 1 cut(s) 416
MlyI GAGTC 2 cut(s) 71, 342
MmeI TCCRAC 3 cut(s) 99, 102, 462
MseI TTAA 3 cut(s) 449, 741, 904
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 876
MspR9I CCNGG 2 cut(s) 759, 876
MvaI CCWGG 1 cut(s) 759
MwoI GCNNNNNNNGC 2 cut(s) 98, 379
NciI CCSGG 1 cut(s) 876
NcoI CCATGG 1 cut(s) 145
NdeII GATC 3 cut(s) 156, 467, 536
NlaIII CATG 2 cut(s) 149, 368
NlaIV GGNNCC 3 cut(s) 66, 591, 672
NmuCI GTSAC 2 cut(s) 367, 385
NspI RCATGY 1 cut(s) 368
PciI ACATGT 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 455
PfeI GAWTC 2 cut(s) 129, 275
PkrI GCNGC 7 cut(s) 247, 268, 411, 547, 550, 812, 890
PleI GAGTC 2 cut(s) 71, 341
PpsI GAGTC 2 cut(s) 71, 341
PpuMI RGGWCCY 1 cut(s) 64
PscI ACATGT 1 cut(s) 364
Psp124BI GAGCTC 1 cut(s) 492
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 757
PspGI CCWGG 1 cut(s) 757
PspN4I GGNNCC 3 cut(s) 66, 591, 672
PspPI GGNCC 2 cut(s) 64, 512
PspPPI RGGWCCY 1 cut(s) 64
SacI GAGCTC 1 cut(s) 492
SapI GCTCTTC 1 cut(s) 455
SaqAI TTAA 3 cut(s) 449, 741, 904
SatI GCNGC 7 cut(s) 246, 267, 410, 546, 549, 811, 889
Sau3AI GATC 3 cut(s) 156, 467, 536
Sau96I GGNCC 2 cut(s) 64, 512
SchI GAGTC 2 cut(s) 71, 342
ScrFI CCNGG 2 cut(s) 759, 876
SduI GDGCHC 2 cut(s) 144, 492
SfaNI GCATC 2 cut(s) 253, 845
SinI GGWCC 1 cut(s) 64
Sse9I AATT 1 cut(s) 416
SsiI CCGC 7 cut(s) 83, 90, 99, 106, 150, 248, 465
SspI AATATT 1 cut(s) 655
SspMI CTAG 3 cut(s) 288, 567, 690
SstI GAGCTC 1 cut(s) 492
StyD4I CCNGG 2 cut(s) 757, 874
StyI CCWWGG 2 cut(s) 145, 897
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 1 cut(s) 56
TaqI TCGA 1 cut(s) 535
TasI AATT 1 cut(s) 416
TfiI GAWTC 2 cut(s) 129, 275
Tru1I TTAA 3 cut(s) 449, 741, 904
Tru9I TTAA 3 cut(s) 449, 741, 904
TscAI CASTG 7 cut(s) 21, 43, 166, 358, 394, 499, 670
TseFI GTSAC 2 cut(s) 367, 385
TseI GCWGC 7 cut(s) 245, 266, 409, 545, 548, 810, 888
Tsp45I GTSAC 2 cut(s) 367, 385
TspDTI ATGAA 3 cut(s) 180, 375, 818
TspRI CASTG 7 cut(s) 21, 43, 166, 358, 394, 499, 670
VpaK11BI GGWCC 1 cut(s) 64
XagI CCTNNNNNAGG 2 cut(s) 201, 639
XceI RCATGY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 764
XspI CTAG 3 cut(s) 288, 567, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.