Rh2DG255500

Protein of unknown function (DUF4057)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009044
Physical Location & Seq
Reverse (-)
27158865 .. 27161379
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG255500.1

Sequence Viewer

Length: 630 bp
ATGGCCACACCAGTGAGAAAGCCTCGCCGCGACACCGCCGACATGCTGACGTGGCAAGAGGTCCCCCGCTCCGACTCTCCCGCCACCGCCTCCTCCGCTCACCGCTCTCACCAGCCGTCGGATGGGATCAGCAAGGTGCTCCACGGCGGTCAGATCACCGAGGAAGAAGCTCAGAGCCTGAACAAAAAGAAGCCTTGCTCCGGGTACAAAATGAAGGAGATTTCCGGAAGCGGGATATTCGCGGCTGGAGAAGGCAATGCGGAGCCGGAGCCGGGTCGGGTTTACCAGCAGGCGGTGAAAGGGATCAGTCAAATCTCGTTCAGCGTTGACGAGCACCTGAGCCCGAAGAAGCCGACGAGTGTTCCGGAGGTGGCGAAGCAGAAGGAGCTGAGTGGGACTTTGCAGTCGGAATTGGATTCCAAGACTCAGAAGGGGATTTCAAATGCCAAGACCAAGGAGCTTAGTGGAAATGACATCTTTGGCCCTCCCCCGGAGATTGTTCCCCGATCGGTCAATGCTGTTCACACCCACGAGTATAAGCAGAGTAAAGATTCCGGGGAGCCGGCGCCCAGAAATCTTCGCACCTCTGTCAAAGTTTCCAATGTGAGTGACTTTATGGTTTCTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4057

Protein Analysis

209

Amino Acids

22.57

Weight (kDa)

8.89

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4057 PF13266 2 - 202 7.3e-88 Protein of unknown function (DUF4057)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 565
AccBSI CCGCTC 3 cut(s) 69, 98, 105
AccII CGCG 2 cut(s) 30, 242
AccIII TCCGGA 2 cut(s) 224, 364
AclWI GGATC 2 cut(s) 134, 311
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 7 cut(s) 118, 122, 200, 231, 272, 292, 490
AgsI TTSAA 1 cut(s) 441
AjiI CACGTC 1 cut(s) 51
AleI CACNNNNGTG 1 cut(s) 11
AluBI AGCT 3 cut(s) 170, 388, 460
AluI AGCT 3 cut(s) 170, 388, 460
Alw21I GWGCWC 2 cut(s) 141, 336
AlwI GGATC 2 cut(s) 134, 311
AlwNI CAGNNNCTG 1 cut(s) 178
Aor13HI TCCGGA 2 cut(s) 224, 364
AoxI GGCC 2 cut(s) 3, 481
AspLEI GCGC 1 cut(s) 568
AspS9I GGNCC 2 cut(s) 61, 482
AsuC2I CCSGG 4 cut(s) 202, 273, 491, 556
AsuHPI GGTGA 4 cut(s) 92, 101, 148, 307
AvaII GGWCC 1 cut(s) 61
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 344
BauI CACGAG 1 cut(s) 530
Bbv12I GWGCWC 2 cut(s) 141, 336
BccI CCATC 1 cut(s) 116
BceAI ACGGC 2 cut(s) 100, 160
BcnI CCSGG 4 cut(s) 202, 273, 491, 556
BfoI RGCGCY 1 cut(s) 569
BisI GCNGC 2 cut(s) 28, 243
BlsI GCNGC 2 cut(s) 29, 244
Bme1390I CCNGG 4 cut(s) 202, 273, 491, 556
Bme18I GGWCC 1 cut(s) 61
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 2 cut(s) 61, 482
BmiI GGNNCC 5 cut(s) 63, 264, 270, 561, 567
BmrFI CCNGG 4 cut(s) 202, 273, 491, 556
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 267
Bpu10I CCTNAGC 1 cut(s) 338
BpuMI CCSGG 4 cut(s) 202, 273, 491, 556
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 5 cut(s) 142, 159, 453, 489, 555
BsaWI WCCGGW 2 cut(s) 224, 364
Bsc4I CCNNNNNNNGG 7 cut(s) 118, 122, 200, 231, 272, 292, 490
Bse118I RCCGGY 1 cut(s) 562
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 262
BseAI TCCGGA 2 cut(s) 224, 364
BseDI CCNNGG 5 cut(s) 142, 159, 453, 489, 555
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 7 cut(s) 118, 122, 200, 231, 272, 292, 490
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 4 cut(s) 185, 329, 380, 440
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 1 cut(s) 82
Bsh1236I CGCG 2 cut(s) 30, 242
Bsh1285I CGRYCG 1 cut(s) 509
BshFI GGCC 2 cut(s) 5, 483
BshNI GGYRCC 1 cut(s) 565
BsiEI CGRYCG 1 cut(s) 509
BsiHKAI GWGCWC 2 cut(s) 141, 336
BsiSI CCGG 8 cut(s) 201, 225, 266, 272, 365, 491, 555, 563
BslFI GGGAC 2 cut(s) 47, 409
BslI CCNNNNNNNGG 7 cut(s) 118, 122, 200, 231, 272, 292, 490
BsmFI GGGAC 2 cut(s) 47, 409
BsnI GGCC 2 cut(s) 5, 483
Bsp1286I GDGCHC 3 cut(s) 141, 336, 344
Bsp13I TCCGGA 2 cut(s) 224, 364
Bsp143I GATC 4 cut(s) 126, 153, 303, 506
BspANI GGCC 2 cut(s) 5, 483
BspCNI CTCAG 4 cut(s) 184, 330, 381, 439
BspEI TCCGGA 2 cut(s) 224, 364
BspFNI CGCG 2 cut(s) 30, 242
BspLI GGNNCC 5 cut(s) 63, 264, 270, 561, 567
BspPI GGATC 2 cut(s) 134, 311
BspT107I GGYRCC 1 cut(s) 565
BsrBI CCGCTC 3 cut(s) 69, 98, 105
BsrDI GCAATG 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 562
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 562
BssECI CCNNGG 5 cut(s) 142, 159, 453, 489, 555
BssMI GATC 4 cut(s) 126, 153, 303, 506
BssNI GRCGYC 1 cut(s) 566
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 530
BstACI GRCGYC 1 cut(s) 566
BstC8I GCNNGC 2 cut(s) 291, 564
BstDEI CTNAG 5 cut(s) 171, 338, 389, 426, 461
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 2 cut(s) 30, 242
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 1 cut(s) 568
BstKTI GATC 4 cut(s) 129, 156, 306, 509
BstMBI GATC 4 cut(s) 126, 153, 303, 506
BstMCI CGRYCG 1 cut(s) 509
BstMWI GCNNNNNNNGC 3 cut(s) 52, 95, 385
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 4 cut(s) 200, 271, 489, 554
BstUI CGCG 2 cut(s) 30, 242
BsuRI GGCC 2 cut(s) 5, 483
BtgI CCRYGG 1 cut(s) 142
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 2 cut(s) 291, 564
CaiI CAGNNNCTG 1 cut(s) 178
CfoI GCGC 1 cut(s) 568
Cfr10I RCCGGY 1 cut(s) 562
Cfr13I GGNCC 2 cut(s) 61, 482
Csp6I GTAC 1 cut(s) 205
CviAII CATG 1 cut(s) 43
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 5 cut(s) 171, 338, 389, 426, 461
DinI GGCGCC 1 cut(s) 567
DpnI GATC 4 cut(s) 128, 155, 305, 508
DpnII GATC 4 cut(s) 126, 153, 303, 506
DrdI GACNNNNNNGTC 1 cut(s) 403
DseDI GACNNNNNNGTC 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 344
EgeI GGCGCC 1 cut(s) 567
EheI GGCGCC 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 1 cut(s) 46
FaiI YATR 3 cut(s) 44, 537, 617
FaqI GGGAC 2 cut(s) 47, 409
FatI CATG 1 cut(s) 42
FauI CCCGC 3 cut(s) 74, 88, 224
Fnu4HI GCNGC 2 cut(s) 28, 243
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 2 cut(s) 28, 243
GlaI GCGC 1 cut(s) 567
GluI GCNGC 2 cut(s) 28, 243
GsuI CTGGAG 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 569
HaeIII GGCC 2 cut(s) 5, 483
HapII CCGG 8 cut(s) 201, 225, 266, 272, 365, 491, 555, 563
HhaI GCGC 1 cut(s) 568
Hin1I GRCGYC 1 cut(s) 566
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 566
HinP1I GCGC 1 cut(s) 566
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 4 cut(s) 74, 416, 424, 551
HpaII CCGG 8 cut(s) 201, 225, 266, 272, 365, 491, 555, 563
HphI GGTGA 4 cut(s) 92, 101, 148, 307
Hpy166II GTNNAC 3 cut(s) 283, 328, 523
Hpy188I TCNGA 6 cut(s) 73, 121, 153, 174, 409, 429
Hpy188III TCNNGA 2 cut(s) 225, 365
Hpy8I GTNNAC 3 cut(s) 283, 328, 523
Hpy99I CGWCG 2 cut(s) 121, 358
HpyAV CCTTC 4 cut(s) 208, 245, 376, 424
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 1 cut(s) 403
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 95, 385
HpyF3I CTNAG 5 cut(s) 171, 338, 389, 426, 461
HpySE526I ACGT 1 cut(s) 50
Hsp92I GRCGYC 1 cut(s) 566
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 566
KasI GGCGCC 1 cut(s) 565
Kpn2I TCCGGA 2 cut(s) 224, 364
KroI GCCGGC 1 cut(s) 562
KroNI GCCGGC 1 cut(s) 564
Kzo9I GATC 4 cut(s) 126, 153, 303, 506
LmnI GCTCC 8 cut(s) 74, 144, 203, 262, 268, 385, 457, 559
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 608
MalI GATC 4 cut(s) 128, 155, 305, 508
MbiI CCGCTC 3 cut(s) 69, 98, 105
MboI GATC 4 cut(s) 126, 153, 303, 506
MboII GAAGA 3 cut(s) 176, 358, 569
MhlI GDGCHC 3 cut(s) 141, 336, 344
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 410
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 566
MlyI GAGTC 2 cut(s) 68, 418
MmeI TCCRAC 3 cut(s) 96, 99, 387
MnlI CCTC 8 cut(s) 33, 52, 100, 103, 154, 361, 495, 595
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 224, 364
MroNI GCCGGC 1 cut(s) 562
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 8 cut(s) 201, 225, 266, 272, 365, 491, 555, 563
MspR9I CCNGG 4 cut(s) 202, 273, 491, 556
MvnI CGCG 2 cut(s) 30, 242
MwoI GCNNNNNNNGC 3 cut(s) 52, 95, 385
NaeI GCCGGC 1 cut(s) 564
NarI GGCGCC 1 cut(s) 566
NciI CCSGG 4 cut(s) 202, 273, 491, 556
NdeII GATC 4 cut(s) 126, 153, 303, 506
NgoMIV GCCGGC 1 cut(s) 562
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 5 cut(s) 63, 264, 270, 561, 567
NmuCI GTSAC 1 cut(s) 608
NspI RCATGY 1 cut(s) 46
OliI CACNNNNGTG 1 cut(s) 11
PdiI GCCGGC 1 cut(s) 564
PfeI GAWTC 2 cut(s) 416, 551
PkrI GCNGC 2 cut(s) 29, 244
Ple19I CGATCG 1 cut(s) 509
PleI GAGTC 2 cut(s) 68, 418
PluTI GGCGCC 1 cut(s) 569
PpsI GAGTC 2 cut(s) 68, 418
PpuMI RGGWCCY 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 61
PspN4I GGNNCC 5 cut(s) 63, 264, 270, 561, 567
PspPI GGNCC 2 cut(s) 61, 482
PspPPI RGGWCCY 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 178
PvuI CGATCG 1 cut(s) 509
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 11
SatI GCNGC 2 cut(s) 28, 243
Sau3AI GATC 4 cut(s) 126, 153, 303, 506
Sau96I GGNCC 2 cut(s) 61, 482
SchI GAGTC 2 cut(s) 68, 418
ScrFI CCNGG 4 cut(s) 202, 273, 491, 556
SduI GDGCHC 3 cut(s) 141, 336, 344
SetI ASST 9 cut(s) 53, 63, 138, 172, 339, 372, 390, 462, 587
SfoI GGCGCC 1 cut(s) 567
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 11
Sse9I AATT 1 cut(s) 410
SspDI GGCGCC 1 cut(s) 565
StyD4I CCNGG 4 cut(s) 200, 271, 489, 554
StyI CCWWGG 1 cut(s) 453
TaiI ACGT 1 cut(s) 53
TaqII GACCGA 1 cut(s) 499
TasI AATT 1 cut(s) 410
TauI GCSGC 2 cut(s) 30, 245
TfiI GAWTC 2 cut(s) 416, 551
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 608
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 1 cut(s) 227
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 61
XceI RCATGY 1 cut(s) 46
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.