Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004650

Physical Location & Seq

Genomic Coordinates Reverse (-)

10632540 .. 10635781

Gene Structure 3242 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1029100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 951 bp

ATGGCGAGTTGCAGGGTGCCAGATGTGGTTCCTTCTCCAGTGGAAGACGTTGAGCAGCTCAGGAAAGCTTTTGCAGGATGGGGCACAAATGAGGGATTGATCATATCCATTCTGACTCACAGGAATGCAGCTCAGAGAAAATTGATTCAGCAGACATACGCCGAAACCTATGGGGAAGATCTTCTCAAGTCGTTGGACAAAGAACTCTCAAGTGATTTTGAGAGGGCTGTGGTGCTGTGGACTCTGGCTCCTGCTGAGAGAGATGCATTTTTGGCTAATGAATCTACGAAGTTGTTGACTAAAAACTATTGGATACTCTTGGAAATAGCTACAACTAGGTCTTCACATGACCTGTACCAGGTAAAGCTGGCGTATCATGCCCGTTACAAGAAATCCCTTGAAGAGGATGTGGCATATCATACATCTGGAGATGTCCGCAAGCTTTTGGTTCCTCTTTTGAGCACTTTCCGATACGAGGGAGATGATATTAACGTGCCCTTGTCAAAGAAAGAAGCTAAGATACTTCATGAGAAAATCTCAGACAAAGCCTACAATGATGAGGAGCTCATCAGGATCCTCACTACCAGGAGCAAAGCACAGCTGAATGCAACCCTCAATCAGTACAACAACGAGTTTGGAAACGCCATTAACAAGGATTTGAAGGCTGACAAAGATGATGAGTTCCTCAAACTACTAAGACAAACAATCAAAAGCTTGACCTTCCCTGAGAAATACTTTGAGAAGGTTCTTCGACTGGGCATCAACAAGCTTGGGACAGAGGAAGGGGCGCTTACTAGAGTTATTACCACCCGGGCAGAGGTTGACTTGCAGCGCATCAAAGAAGAATACTACCGGAGGAACAGCGTTCATTTGGACCACGCAATTGTCAAGGACACTTCCGGAGACTATGAGAAGTTTCTTGTGGAACTGGTTGGACATGCAGATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006810 GO:0006950 GO:0006970 GO:0008150 GO:0008643 GO:0009266 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009579 GO:0009628 GO:0009651 GO:0010035 GO:0015774 GO:0016020 GO:0033036 GO:0033037 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050896 GO:0051179 GO:0051234 GO:0071702 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Annexin

Protein Analysis

317

Amino Acids

36.19

Weight (kDa)

6.12

Isoelectric Point (pI)

32.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Annexin	PF00191	16 - 79	1.9e-21	Annexin
Annexin	PF00191	88 - 152	1.3e-13	Annexin
Annexin	PF00191	171 - 232	7.2e-12	Annexin
Annexin	PF00191	245 - 310	2.8e-16	Annexin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004650)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35720 AT5G65020 AT5G65020
fragaria_vesca	FvH4_1g20930 FvH4_1g20930 FvH4_1g20930 FvH4_1g20930 FvH4_1g20930 FvH4_1g20930 FvH4_1g20930
malus_domestica	MD01G1029100.v1.1 MD04G1000900.v1.1
prunus_persica	Prupe.6G186600_v2.0.a1
pyrus_communis	pycom01g05720
rosa_chinensis	RchiOBHm_Chr2g0114411 RchiOBHm_Chr2g0114431
rosa_laevigata	RLG00000018099 RLG00000018100
rosa_multiflora	Rmu_co8507663.1_g000001 Rmu_co8512843.1_g000002 Rmu_sc0000431.1_g000032
rosa_roxburghii	Rroxscaffold_2G00130130 Rroxscaffold_2G00130140
rosa_rugosa	Rorug02G0190300
rosa_samantha	Rh2AG247400 Rh2BG257200 Rh2BG257300 Rh2CG250900 Rh2DG255400
rosa_wichuraiana	Rw2G019220 Rw2G019230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	16
AccIII	TCCGGA	1 cut(s)	899
AciI	CCGC	1 cut(s)	436
AclWI	GGATC	2 cut(s)	568, 581
AfaI	GTAC	2 cut(s)	356, 623
AfiI	CCNNNNNNNGG	4 cut(s)	358, 403, 475, 817
AgsI	TTSAA	2 cut(s)	401, 661
AjnI	CCWGG	2 cut(s)	357, 584
Alw21I	GWGCWC	2 cut(s)	464, 567
Alw26I	GTCTC	1 cut(s)	897
AlwI	GGATC	2 cut(s)	568, 581
Ama87I	CYCGRG	1 cut(s)	810
Aor13HI	TCCGGA	1 cut(s)	899
ApeKI	GCWGC	3 cut(s)	55, 128, 829
Asp700I	GAANNNNTTC	1 cut(s)	180
AspLEI	GCGC	2 cut(s)	790, 834
AspS9I	GGNCC	1 cut(s)	874
AsuC2I	CCSGG	2 cut(s)	811, 812
AvaI	CYCGRG	1 cut(s)	810
AvaII	GGWCC	1 cut(s)	874
BaeGI	GKGCMC	2 cut(s)	86, 498
BamHI	GGATCC	1 cut(s)	573
BanI	GGYRCC	1 cut(s)	16
BanII	GRGCYC	1 cut(s)	567
BarI	GAAGNNNNNNTAC	2 cut(s)	504, 536
BbsI	GAAGAC	2 cut(s)	51, 333
Bbv12I	GWGCWC	2 cut(s)	464, 567
BbvI	GCAGC	3 cut(s)	67, 140, 841
BccI	CCATC	1 cut(s)	72
BciT130I	CCWGG	2 cut(s)	359, 586
BciVI	GTATCC	1 cut(s)	306
BclI	TGATCA	1 cut(s)	99
BcnI	CCSGG	2 cut(s)	811, 812
BcoDI	GTCTC	1 cut(s)	897
BfaI	CTAG	2 cut(s)	336, 795
BfoI	RGCGCY	1 cut(s)	791
BfuI	GTATCC	1 cut(s)	306
BglII	AGATCT	1 cut(s)	178
BisI	GCNGC	3 cut(s)	56, 129, 830
BlsI	GCNGC	3 cut(s)	57, 130, 831
Bme1390I	CCNGG	4 cut(s)	359, 586, 811, 812
Bme18I	GGWCC	1 cut(s)	874
BmeT110I	CYCGRG	1 cut(s)	810
BmgT120I	GGNCC	1 cut(s)	874
BmiI	GGNNCC	5 cut(s)	18, 30, 249, 450, 575
BmrFI	CCNGG	4 cut(s)	359, 586, 811, 812
BmrI	ACTGGG	1 cut(s)	764
BmsI	GCATC	4 cut(s)	253, 768, 843, 934
BmuI	ACTGGG	1 cut(s)	764
BpiI	GAAGAC	2 cut(s)	51, 333
BplI	GAGNNNNNCTC	2 cut(s)	521, 553
BpmI	CTGGAG	2 cut(s)	21, 447
Bpu10I	CCTNAGC	1 cut(s)	59
BpuEI	CTTGAG	2 cut(s)	170, 193
BpuMI	CCSGG	2 cut(s)	811, 812
BsaJI	CCNNGG	1 cut(s)	810
BsaWI	WCCGGW	2 cut(s)	852, 899
BsaXI	ACNNNNNCTCC	2 cut(s)	232, 262
Bsc4I	CCNNNNNNNGG	4 cut(s)	358, 403, 475, 817
Bse1I	ACTGG	3 cut(s)	38, 759, 933
BseAI	TCCGGA	1 cut(s)	899
BseBI	CCWGG	2 cut(s)	359, 586
BseDI	CCNNGG	1 cut(s)	810
BseGI	GGATG	2 cut(s)	83, 412
BseLI	CCNNNNNNNGG	4 cut(s)	358, 403, 475, 817
BseMII	CTCAG	5 cut(s)	73, 146, 246, 552, 717
BseNI	ACTGG	3 cut(s)	38, 759, 933
BseRI	GAGGAG	1 cut(s)	575
BseSI	GKGCMC	2 cut(s)	86, 498
BseXI	GCAGC	3 cut(s)	67, 140, 841
BshNI	GGYRCC	1 cut(s)	16
BsiHKAI	GWGCWC	2 cut(s)	464, 567
BsiHKCI	CYCGRG	1 cut(s)	810
BsiSI	CCGG	3 cut(s)	811, 853, 900
BslFI	GGGAC	1 cut(s)	787
BslI	CCNNNNNNNGG	4 cut(s)	358, 403, 475, 817
BsmAI	GTCTC	1 cut(s)	897
BsmFI	GGGAC	1 cut(s)	787
BsmI	GAATGC	2 cut(s)	130, 610
BsoBI	CYCGRG	1 cut(s)	810
Bsp1286I	GDGCHC	4 cut(s)	86, 464, 498, 567
Bsp13I	TCCGGA	1 cut(s)	899
Bsp143I	GATC	3 cut(s)	99, 178, 573
BspACI	CCGC	1 cut(s)	436
BspCNI	CTCAG	5 cut(s)	72, 145, 247, 551, 718
BspEI	TCCGGA	1 cut(s)	899
BspHI	TCATGA	1 cut(s)	526
BspLI	GGNNCC	5 cut(s)	18, 30, 249, 450, 575
BspPI	GGATC	2 cut(s)	568, 581
BspT107I	GGYRCC	1 cut(s)	16
BsrI	ACTGG	3 cut(s)	38, 759, 933
BssECI	CCNNGG	1 cut(s)	810
BssMI	GATC	3 cut(s)	99, 178, 573
Bst2UI	CCWGG	2 cut(s)	359, 586
Bst6I	CTCTTC	1 cut(s)	396
BstC8I	GCNNGC	2 cut(s)	369, 440
BstDEI	CTNAG	7 cut(s)	59, 132, 255, 516, 538, 695, 726
BstENI	CCTNNNNNAGG	2 cut(s)	356, 401
BstF5I	GGATG	2 cut(s)	83, 412
BstH2I	RGCGCY	1 cut(s)	791
BstHHI	GCGC	2 cut(s)	790, 834
BstKTI	GATC	3 cut(s)	102, 181, 576
BstMAI	GTCTC	1 cut(s)	897
BstMBI	GATC	3 cut(s)	99, 178, 573
BstMWI	GCNNNNNNNGC	2 cut(s)	272, 377
BstNI	CCWGG	2 cut(s)	359, 586
BstNSI	RCATGY	1 cut(s)	941
BstSCI	CCNGG	4 cut(s)	357, 584, 809, 810
BstSLI	GKGCMC	2 cut(s)	86, 498
BstV1I	GCAGC	3 cut(s)	67, 140, 841
BstV2I	GAAGAC	2 cut(s)	51, 333
BstX2I	RGATCY	2 cut(s)	178, 573
BstYI	RGATCY	2 cut(s)	178, 573
BsuI	GTATCC	1 cut(s)	306
BtsCI	GGATG	2 cut(s)	83, 412
BtsIMutI	CAGTG	1 cut(s)	45
Cac8I	GCNNGC	2 cut(s)	369, 440
CciI	TCATGA	1 cut(s)	526
CfoI	GCGC	2 cut(s)	790, 834
Cfr13I	GGNCC	1 cut(s)	874
Cfr9I	CCCGGG	1 cut(s)	810
CsiI	ACCWGGT	1 cut(s)	357
Csp6I	GTAC	2 cut(s)	355, 622
CviAII	CATG	4 cut(s)	347, 377, 527, 938
CviQI	GTAC	2 cut(s)	355, 622
DdeI	CTNAG	7 cut(s)	59, 132, 255, 516, 538, 695, 726
DpnI	GATC	3 cut(s)	101, 180, 575
DpnII	GATC	3 cut(s)	99, 178, 573
Eam1104I	CTCTTC	1 cut(s)	396
EarI	CTCTTC	1 cut(s)	396
Ecl136II	GAGCTC	1 cut(s)	565
Eco24I	GRGCYC	1 cut(s)	567
Eco47I	GGWCC	1 cut(s)	874
Eco53kI	GAGCTC	1 cut(s)	565
Eco88I	CYCGRG	1 cut(s)	810
EcoICRI	GAGCTC	1 cut(s)	565
EcoNI	CCTNNNNNAGG	2 cut(s)	356, 401
EcoRII	CCWGG	2 cut(s)	357, 584
EcoT22I	ATGCAT	1 cut(s)	268
EcoT38I	GRGCYC	1 cut(s)	567
FaeI	CATG	4 cut(s)	350, 380, 530, 941
FalI	AAGNNNNNCTT	2 cut(s)	774, 806
FaqI	GGGAC	1 cut(s)	787
FatI	CATG	4 cut(s)	346, 376, 526, 937
FbaI	TGATCA	1 cut(s)	99
Fnu4HI	GCNGC	3 cut(s)	56, 129, 830
FokI	GGATG	2 cut(s)	90, 419
FriOI	GRGCYC	1 cut(s)	567
Fsp4HI	GCNGC	3 cut(s)	56, 129, 830
FspBI	CTAG	2 cut(s)	336, 795
GlaI	GCGC	2 cut(s)	789, 833
GluI	GCNGC	3 cut(s)	56, 129, 830
GsuI	CTGGAG	2 cut(s)	21, 447
HaeII	RGCGCY	1 cut(s)	791
HapII	CCGG	3 cut(s)	811, 853, 900
HhaI	GCGC	2 cut(s)	790, 834
Hin1II	CATG	4 cut(s)	350, 380, 530, 941
Hin6I	GCGC	2 cut(s)	788, 832
HinP1I	GCGC	2 cut(s)	788, 832
HincII	GTYRAC	2 cut(s)	297, 823
HindII	GTYRAC	2 cut(s)	297, 823
HindIII	AAGCTT	4 cut(s)	66, 440, 712, 767
HinfI	GANTC	4 cut(s)	115, 145, 241, 281
HpaII	CCGG	3 cut(s)	811, 853, 900
Hpy166II	GTNNAC	3 cut(s)	240, 297, 823
Hpy188I	TCNGA	4 cut(s)	114, 135, 470, 541
Hpy188III	TCNNGA	5 cut(s)	61, 426, 527, 571, 900
Hpy8I	GTNNAC	3 cut(s)	240, 297, 823
HpyAV	CCTTC	5 cut(s)	42, 655, 730, 736, 776
HpyCH4IV	ACGT	2 cut(s)	48, 492
HpyCH4V	TGCA	7 cut(s)	12, 74, 128, 266, 608, 829, 941
HpyF10VI	GCNNNNNNNGC	2 cut(s)	272, 377
HpyF3I	CTNAG	7 cut(s)	59, 132, 255, 516, 538, 695, 726
HpySE526I	ACGT	2 cut(s)	48, 492
Hsp92II	CATG	4 cut(s)	350, 380, 530, 941
HspAI	GCGC	2 cut(s)	788, 832
Kpn2I	TCCGGA	1 cut(s)	899
Ksp22I	TGATCA	1 cut(s)	99
Kzo9I	GATC	3 cut(s)	99, 178, 573
LmnI	GCTCC	3 cut(s)	253, 562, 588
Lsp1109I	GCAGC	3 cut(s)	67, 140, 841
LweI	GCATC	4 cut(s)	253, 768, 843, 934
MabI	ACCWGGT	1 cut(s)	357
MaeI	CTAG	2 cut(s)	336, 795
MaeII	ACGT	2 cut(s)	48, 492
MaeIII	GTNAC	1 cut(s)	383
MalI	GATC	3 cut(s)	101, 180, 575
MboI	GATC	3 cut(s)	99, 178, 573
MboII	GAAGA	7 cut(s)	56, 173, 188, 333, 413, 740, 854
MfeI	CAATTG	1 cut(s)	882
MflI	RGATCY	2 cut(s)	178, 573
MhlI	GDGCHC	4 cut(s)	86, 464, 498, 567
MluCI	AATT	2 cut(s)	140, 882
MlyI	GAGTC	2 cut(s)	109, 235
MmeI	TCCRAC	2 cut(s)	174, 913
Mph1103I	ATGCAT	1 cut(s)	268
MroI	TCCGGA	1 cut(s)	899
MroXI	GAANNNNTTC	1 cut(s)	180
MseI	TTAA	2 cut(s)	489, 648
MslI	CAYNNNNRTG	2 cut(s)	123, 942
MspA1I	CMGCKG	1 cut(s)	601
MspI	CCGG	3 cut(s)	811, 853, 900
MspR9I	CCNGG	4 cut(s)	359, 586, 811, 812
MunI	CAATTG	1 cut(s)	882
Mva1269I	GAATGC	2 cut(s)	130, 610
MvaI	CCWGG	2 cut(s)	359, 586
MwoI	GCNNNNNNNGC	2 cut(s)	272, 377
NciI	CCSGG	2 cut(s)	811, 812
NdeII	GATC	3 cut(s)	99, 178, 573
NlaIII	CATG	4 cut(s)	350, 380, 530, 941
NlaIV	GGNNCC	5 cut(s)	18, 30, 249, 450, 575
NsiI	ATGCAT	1 cut(s)	268
NspI	RCATGY	1 cut(s)	941
PagI	TCATGA	1 cut(s)	526
PctI	GAATGC	2 cut(s)	130, 610
PdmI	GAANNNNTTC	1 cut(s)	180
PfeI	GAWTC	2 cut(s)	145, 281
PkrI	GCNGC	3 cut(s)	57, 130, 831
PleI	GAGTC	2 cut(s)	109, 235
PpsI	GAGTC	2 cut(s)	109, 235
Psp124BI	GAGCTC	1 cut(s)	567
Psp6I	CCWGG	2 cut(s)	357, 584
PspGI	CCWGG	2 cut(s)	357, 584
PspN4I	GGNNCC	5 cut(s)	18, 30, 249, 450, 575
PspPI	GGNCC	1 cut(s)	874
PsuI	RGATCY	2 cut(s)	178, 573
PvuII	CAGCTG	1 cut(s)	601
RsaI	GTAC	2 cut(s)	356, 623
RsaNI	GTAC	2 cut(s)	355, 622
RseI	CAYNNNNRTG	2 cut(s)	123, 942
SacI	GAGCTC	1 cut(s)	567
SaqAI	TTAA	2 cut(s)	489, 648
SatI	GCNGC	3 cut(s)	56, 129, 830
Sau3AI	GATC	3 cut(s)	99, 178, 573
Sau96I	GGNCC	1 cut(s)	874
SchI	GAGTC	2 cut(s)	109, 235
ScrFI	CCNGG	4 cut(s)	359, 586, 811, 812
SduI	GDGCHC	4 cut(s)	86, 464, 498, 567
SexAI	ACCWGGT	1 cut(s)	357
SfaNI	GCATC	4 cut(s)	253, 768, 843, 934
SinI	GGWCC	1 cut(s)	874
SmaI	CCCGGG	1 cut(s)	812
SmiMI	CAYNNNNRTG	2 cut(s)	123, 942
SmlI	CTYRAG	2 cut(s)	185, 208
SmoI	CTYRAG	2 cut(s)	185, 208
Sse9I	AATT	2 cut(s)	140, 882
SsiI	CCGC	1 cut(s)	436
SspMI	CTAG	2 cut(s)	336, 795
SstI	GAGCTC	1 cut(s)	567
StyD4I	CCNGG	4 cut(s)	357, 584, 809, 810
TaiI	ACGT	2 cut(s)	51, 495
TaqI	TCGA	1 cut(s)	751
TasI	AATT	2 cut(s)	140, 882
TatI	WGTACW	1 cut(s)	621
TfiI	GAWTC	2 cut(s)	145, 281
Tru1I	TTAA	2 cut(s)	489, 648
Tru9I	TTAA	2 cut(s)	489, 648
TscAI	CASTG	1 cut(s)	45
TseI	GCWGC	3 cut(s)	55, 128, 829
TspDTI	ATGAA	3 cut(s)	294, 515, 857
TspMI	CCCGGG	1 cut(s)	810
TspRI	CASTG	1 cut(s)	45
VpaK11BI	GGWCC	1 cut(s)	874
XagI	CCTNNNNNAGG	2 cut(s)	356, 401
XceI	RCATGY	1 cut(s)	941
XmaI	CCCGGG	1 cut(s)	810
XmnI	GAANNNNTTC	1 cut(s)	180
XspI	CTAG	2 cut(s)	336, 795
Zsp2I	ATGCAT	1 cut(s)	268

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.