Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004650

Physical Location & Seq

Genomic Coordinates Reverse (-)

99101 .. 100007

Gene Structure 907 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1000900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 855 bp

ATGGCGAGTTGCAGGGTGCCAAATGTGGTTCCTTCTCCAGTGGAAGACGCTGAGCAGCTCAGGAAAGCTTTTGCAGGACGGGGCACAAATGAGGGATTGATCATATCCATTCTGACTCACAGGAATGCAGCTCAGAGAAAATTGATTCGGCAGACATACGCCGAAACCGATGGGGAAGATCTTCTCAAGTTGTTGGACAAGGAACTATCGAGTAATTTTGAGAGGGCTGTGGTGCTGTGGACTCTGACTCCTGCTGAAGCCCACAGTGATGAGGAGCTCATCAGGATCCTCACTACCAGGAGCCAAGCACAGCTGAATGCAACCCTCAATCAATACAACAACGAGTTTGGAAACGCCATTAACAAGGAGTTGAAGGCTGACAAAGATGATGAGTTCCTCAAACTACTAAGGCTAACAATCAAAAGATTGACCTTCCCTGAGAAATACTTTGAGAAGGTTCTTCAACTGACCATCAACAAGCTCGAGACAGATAAGGGGGCAAACAGATATATGTACATATATGCATGCAAACAAATATATATACCTATATATACGCATGCAAACAAATATACATATATATGCATGCAGTTCTCGGGACAGATGAGGGGGCAAACAAATATGTACATATATGCATGCAAACAAATATATATATATACGTATACGCATGCAAACAAATATACATGTATATGCATGCAGCTCTCGGGACAGATGGCATGCAAACAAATATACATATATATGCATGCAGCTCCCGGGACGGACAAAGGTATATATATATACATATACGTGCAGCTGACGAACAAACAAACAAGACACCAATCTAAGGATCAGACCTTTTATCCATTCTTTTCGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006810 GO:0006950 GO:0006970 GO:0008150 GO:0008643 GO:0009266 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009579 GO:0009628 GO:0009651 GO:0010035 GO:0015774 GO:0016020 GO:0033036 GO:0033037 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050896 GO:0051179 GO:0051234 GO:0071702 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Annexin

Protein Analysis

285

Amino Acids

33.05

Weight (kDa)

9.04

Isoelectric Point (pI)

47.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Annexin	PF00191	16 - 79	9.2e-19	Annexin
Annexin	PF00191	90 - 136	1.5e-08	Annexin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004650)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35720 AT5G65020 AT5G65020
fragaria_vesca	FvH4_1g20930 FvH4_1g20930 FvH4_1g20930 FvH4_1g20930 FvH4_1g20930 FvH4_1g20930 FvH4_1g20930
malus_domestica	MD01G1029100.v1.1 MD04G1000900.v1.1
prunus_persica	Prupe.6G186600_v2.0.a1
pyrus_communis	pycom01g05720
rosa_chinensis	RchiOBHm_Chr2g0114411 RchiOBHm_Chr2g0114431
rosa_laevigata	RLG00000018099 RLG00000018100
rosa_multiflora	Rmu_co8507663.1_g000001 Rmu_co8512843.1_g000002 Rmu_sc0000431.1_g000032
rosa_roxburghii	Rroxscaffold_2G00130130 Rroxscaffold_2G00130140
rosa_rugosa	Rorug02G0190300
rosa_samantha	Rh2AG247400 Rh2BG257200 Rh2BG257300 Rh2CG250900 Rh2DG255400
rosa_wichuraiana	Rw2G019220 Rw2G019230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	16
AccI	GTMKAC	1 cut(s)	659
AclWI	GGATC	3 cut(s)	280, 293, 831
AcuI	CTGAAG	1 cut(s)	276
AfaI	GTAC	2 cut(s)	515, 623
AfiI	CCNNNNNNNGG	1 cut(s)	820
AflIII	ACRYGT	1 cut(s)	680
AgsI	TTSAA	2 cut(s)	373, 464
AjnI	CCWGG	1 cut(s)	296
AluBI	AGCT	9 cut(s)	58, 68, 131, 277, 313, 481, 697, 746, 790
AluI	AGCT	9 cut(s)	58, 68, 131, 277, 313, 481, 697, 746, 790
Alw21I	GWGCWC	1 cut(s)	279
Alw26I	GTCTC	1 cut(s)	479
AlwI	GGATC	3 cut(s)	280, 293, 831
Ama87I	CYCGRG	4 cut(s)	482, 592, 700, 749
ApeKI	GCWGC	5 cut(s)	55, 128, 694, 743, 787
Asp700I	GAANNNNTTC	1 cut(s)	180
AsuC2I	CCSGG	2 cut(s)	750, 751
AsuII	TTCGAA	1 cut(s)	848
AvaI	CYCGRG	4 cut(s)	482, 592, 700, 749
BaeGI	GKGCMC	1 cut(s)	86
BamHI	GGATCC	1 cut(s)	285
BanI	GGYRCC	1 cut(s)	16
BanII	GRGCYC	1 cut(s)	279
BbsI	GAAGAC	1 cut(s)	51
Bbv12I	GWGCWC	1 cut(s)	279
BbvI	GCAGC	5 cut(s)	67, 140, 706, 755, 799
BccI	CCATC	3 cut(s)	164, 479, 703
BciT130I	CCWGG	1 cut(s)	298
BclI	TGATCA	1 cut(s)	99
BcnI	CCSGG	2 cut(s)	750, 751
BcoDI	GTCTC	1 cut(s)	479
BglII	AGATCT	1 cut(s)	178
BisI	GCNGC	5 cut(s)	56, 129, 695, 744, 788
BlpI	GCTNAGC	1 cut(s)	51
BlsI	GCNGC	5 cut(s)	57, 130, 696, 745, 789
Bme1390I	CCNGG	3 cut(s)	298, 750, 751
BmeT110I	CYCGRG	4 cut(s)	482, 592, 700, 749
BmiI	GGNNCC	4 cut(s)	18, 30, 287, 302
BmrFI	CCNGG	3 cut(s)	298, 750, 751
BpiI	GAAGAC	1 cut(s)	51
BpmI	CTGGAG	1 cut(s)	21
Bpu10I	CCTNAGC	1 cut(s)	59
Bpu1102I	GCTNAGC	1 cut(s)	51
Bpu14I	TTCGAA	1 cut(s)	848
BpuEI	CTTGAG	1 cut(s)	170
BpuMI	CCSGG	2 cut(s)	750, 751
BsaAI	YACGTR	2 cut(s)	657, 784
BsaJI	CCNNGG	1 cut(s)	749
BsaXI	ACNNNNNCTCC	2 cut(s)	232, 262
Bsc4I	CCNNNNNNNGG	1 cut(s)	820
Bse1I	ACTGG	1 cut(s)	38
BseBI	CCWGG	1 cut(s)	298
BseDI	CCNNGG	1 cut(s)	749
BseLI	CCNNNNNNNGG	1 cut(s)	820
BseMII	CTCAG	4 cut(s)	42, 73, 146, 429
BseNI	ACTGG	1 cut(s)	38
BseRI	GAGGAG	1 cut(s)	287
BseSI	GKGCMC	1 cut(s)	86
BseXI	GCAGC	5 cut(s)	67, 140, 706, 755, 799
BsgI	GTGCAG	1 cut(s)	806
BshNI	GGYRCC	1 cut(s)	16
BsiHKAI	GWGCWC	1 cut(s)	279
BsiHKCI	CYCGRG	4 cut(s)	482, 592, 700, 749
BsiSI	CCGG	1 cut(s)	750
BslFI	GGGAC	3 cut(s)	609, 717, 766
BslI	CCNNNNNNNGG	1 cut(s)	820
BsmAI	GTCTC	1 cut(s)	479
BsmFI	GGGAC	3 cut(s)	609, 717, 766
BsmI	GAATGC	2 cut(s)	130, 322
BsoBI	CYCGRG	4 cut(s)	482, 592, 700, 749
Bsp119I	TTCGAA	1 cut(s)	848
Bsp1286I	GDGCHC	2 cut(s)	86, 279
Bsp1407I	TGTACA	2 cut(s)	513, 621
Bsp143I	GATC	4 cut(s)	99, 178, 285, 823
Bsp1720I	GCTNAGC	1 cut(s)	51
BspCNI	CTCAG	4 cut(s)	43, 72, 145, 430
BspLI	GGNNCC	4 cut(s)	18, 30, 287, 302
BspPI	GGATC	3 cut(s)	280, 293, 831
BspT104I	TTCGAA	1 cut(s)	848
BspT107I	GGYRCC	1 cut(s)	16
BsrGI	TGTACA	2 cut(s)	513, 621
BsrI	ACTGG	1 cut(s)	38
BssECI	CCNNGG	1 cut(s)	749
BssMI	GATC	4 cut(s)	99, 178, 285, 823
BssNAI	GTATAC	1 cut(s)	660
Bst1107I	GTATAC	1 cut(s)	660
Bst2UI	CCWGG	1 cut(s)	298
Bst4CI	ACNGT	1 cut(s)	266
BstAUI	TGTACA	2 cut(s)	513, 621
BstBAI	YACGTR	2 cut(s)	657, 784
BstBI	TTCGAA	1 cut(s)	848
BstC8I	GCNNGC	8 cut(s)	526, 558, 584, 634, 666, 692, 715, 741
BstDEI	CTNAG	6 cut(s)	51, 59, 132, 407, 438, 819
BstKTI	GATC	4 cut(s)	102, 181, 288, 826
BstMAI	GTCTC	1 cut(s)	479
BstMBI	GATC	4 cut(s)	99, 178, 285, 823
BstNI	CCWGG	1 cut(s)	298
BstNSI	RCATGY	9 cut(s)	528, 560, 586, 636, 668, 684, 694, 717, 743
BstSCI	CCNGG	3 cut(s)	296, 748, 749
BstSLI	GKGCMC	1 cut(s)	86
BstSNI	TACGTA	1 cut(s)	657
BstV1I	GCAGC	5 cut(s)	67, 140, 706, 755, 799
BstV2I	GAAGAC	1 cut(s)	51
BstX2I	RGATCY	2 cut(s)	178, 285
BstYI	RGATCY	2 cut(s)	178, 285
BstZ17I	GTATAC	1 cut(s)	660
BtsIMutI	CAGTG	2 cut(s)	45, 271
Cac8I	GCNNGC	8 cut(s)	526, 558, 584, 634, 666, 692, 715, 741
Cfr9I	CCCGGG	1 cut(s)	749
CseI	GACGC	1 cut(s)	56
Csp6I	GTAC	2 cut(s)	514, 622
CviAII	CATG	9 cut(s)	525, 557, 583, 633, 665, 681, 691, 714, 740
CviQI	GTAC	2 cut(s)	514, 622
DdeI	CTNAG	6 cut(s)	51, 59, 132, 407, 438, 819
DpnI	GATC	4 cut(s)	101, 180, 287, 825
DpnII	GATC	4 cut(s)	99, 178, 285, 823
Ecl136II	GAGCTC	1 cut(s)	277
Eco105I	TACGTA	1 cut(s)	657
Eco24I	GRGCYC	1 cut(s)	279
Eco53kI	GAGCTC	1 cut(s)	277
Eco57I	CTGAAG	1 cut(s)	276
Eco88I	CYCGRG	4 cut(s)	482, 592, 700, 749
EcoICRI	GAGCTC	1 cut(s)	277
EcoRII	CCWGG	1 cut(s)	296
EcoT22I	ATGCAT	5 cut(s)	526, 584, 634, 692, 741
EcoT38I	GRGCYC	1 cut(s)	279
FaeI	CATG	9 cut(s)	528, 560, 586, 636, 668, 684, 694, 717, 743
FaqI	GGGAC	3 cut(s)	609, 717, 766
FatI	CATG	9 cut(s)	524, 556, 582, 632, 664, 680, 690, 713, 739
FbaI	TGATCA	1 cut(s)	99
FblI	GTMKAC	1 cut(s)	659
Fnu4HI	GCNGC	5 cut(s)	56, 129, 695, 744, 788
FriOI	GRGCYC	1 cut(s)	279
Fsp4HI	GCNGC	5 cut(s)	56, 129, 695, 744, 788
GluI	GCNGC	5 cut(s)	56, 129, 695, 744, 788
GsuI	CTGGAG	1 cut(s)	21
HapII	CCGG	1 cut(s)	750
HgaI	GACGC	1 cut(s)	56
Hin1II	CATG	9 cut(s)	528, 560, 586, 636, 668, 684, 694, 717, 743
HindIII	AAGCTT	1 cut(s)	66
HinfI	GANTC	4 cut(s)	115, 145, 241, 247
HpaII	CCGG	1 cut(s)	750
Hpy166II	GTNNAC	2 cut(s)	240, 660
Hpy188I	TCNGA	4 cut(s)	114, 135, 246, 828
Hpy188III	TCNNGA	5 cut(s)	61, 283, 484, 594, 702
Hpy8I	GTNNAC	2 cut(s)	240, 660
HpyAV	CCTTC	4 cut(s)	42, 367, 442, 448
HpyCH4III	ACNGT	1 cut(s)	266
HpyCH4IV	ACGT	2 cut(s)	656, 783
HpyF3I	CTNAG	6 cut(s)	51, 59, 132, 407, 438, 819
HpySE526I	ACGT	2 cut(s)	656, 783
Hsp92II	CATG	9 cut(s)	528, 560, 586, 636, 668, 684, 694, 717, 743
Ksp22I	TGATCA	1 cut(s)	99
Kzo9I	GATC	4 cut(s)	99, 178, 285, 823
LmnI	GCTCC	3 cut(s)	274, 300, 751
Lsp1109I	GCAGC	5 cut(s)	67, 140, 706, 755, 799
MaeII	ACGT	2 cut(s)	656, 783
MalI	GATC	4 cut(s)	101, 180, 287, 825
MboI	GATC	4 cut(s)	99, 178, 285, 823
MboII	GAAGA	4 cut(s)	56, 173, 188, 452
MflI	RGATCY	2 cut(s)	178, 285
MhlI	GDGCHC	2 cut(s)	86, 279
MluCI	AATT	2 cut(s)	140, 214
MlyI	GAGTC	3 cut(s)	109, 235, 241
MmeI	TCCRAC	1 cut(s)	174
MnlI	CCTC	7 cut(s)	85, 216, 265, 299, 335, 407, 597
Mph1103I	ATGCAT	5 cut(s)	526, 584, 634, 692, 741
MroXI	GAANNNNTTC	1 cut(s)	180
MseI	TTAA	1 cut(s)	360
MslI	CAYNNNNRTG	6 cut(s)	123, 267, 577, 685, 734, 782
MspA1I	CMGCKG	2 cut(s)	313, 790
MspI	CCGG	1 cut(s)	750
MspR9I	CCNGG	3 cut(s)	298, 750, 751
Mva1269I	GAATGC	2 cut(s)	130, 322
MvaI	CCWGG	1 cut(s)	298
NciI	CCSGG	2 cut(s)	750, 751
NdeII	GATC	4 cut(s)	99, 178, 285, 823
NlaIII	CATG	9 cut(s)	528, 560, 586, 636, 668, 684, 694, 717, 743
NlaIV	GGNNCC	4 cut(s)	18, 30, 287, 302
NsiI	ATGCAT	5 cut(s)	526, 584, 634, 692, 741
NspI	RCATGY	9 cut(s)	528, 560, 586, 636, 668, 684, 694, 717, 743
NspV	TTCGAA	1 cut(s)	848
PaeI	GCATGC	8 cut(s)	528, 560, 586, 636, 668, 694, 717, 743
PaeR7I	CTCGAG	1 cut(s)	482
PciI	ACATGT	1 cut(s)	680
PcsI	WCGNNNNNNNCGW	1 cut(s)	165
PctI	GAATGC	2 cut(s)	130, 322
PdmI	GAANNNNTTC	1 cut(s)	180
PfeI	GAWTC	1 cut(s)	145
PkrI	GCNGC	5 cut(s)	57, 130, 696, 745, 789
PleI	GAGTC	3 cut(s)	109, 235, 241
PpsI	GAGTC	3 cut(s)	109, 235, 241
Ppu21I	YACGTR	2 cut(s)	657, 784
PscI	ACATGT	1 cut(s)	680
Psp124BI	GAGCTC	1 cut(s)	279
Psp6I	CCWGG	1 cut(s)	296
PspGI	CCWGG	1 cut(s)	296
PspN4I	GGNNCC	4 cut(s)	18, 30, 287, 302
PsuI	RGATCY	2 cut(s)	178, 285
PvuII	CAGCTG	2 cut(s)	313, 790
RsaI	GTAC	2 cut(s)	515, 623
RsaNI	GTAC	2 cut(s)	514, 622
RseI	CAYNNNNRTG	6 cut(s)	123, 267, 577, 685, 734, 782
SacI	GAGCTC	1 cut(s)	279
SaqAI	TTAA	1 cut(s)	360
SatI	GCNGC	5 cut(s)	56, 129, 695, 744, 788
Sau3AI	GATC	4 cut(s)	99, 178, 285, 823
SchI	GAGTC	3 cut(s)	109, 235, 241
ScrFI	CCNGG	3 cut(s)	298, 750, 751
SduI	GDGCHC	2 cut(s)	86, 279
Sfr274I	CTCGAG	1 cut(s)	482
SfuI	TTCGAA	1 cut(s)	848
SlaI	CTCGAG	1 cut(s)	482
SmaI	CCCGGG	1 cut(s)	751
SmiMI	CAYNNNNRTG	6 cut(s)	123, 267, 577, 685, 734, 782
SmlI	CTYRAG	2 cut(s)	185, 482
SmoI	CTYRAG	2 cut(s)	185, 482
SnaBI	TACGTA	1 cut(s)	657
SphI	GCATGC	8 cut(s)	528, 560, 586, 636, 668, 694, 717, 743
Sse9I	AATT	2 cut(s)	140, 214
SstI	GAGCTC	1 cut(s)	279
StyD4I	CCNGG	3 cut(s)	296, 748, 749
TaaI	ACNGT	1 cut(s)	266
TaiI	ACGT	2 cut(s)	659, 786
TaqI	TCGA	3 cut(s)	209, 483, 848
TasI	AATT	2 cut(s)	140, 214
TatI	WGTACW	2 cut(s)	513, 621
TfiI	GAWTC	1 cut(s)	145
Tru1I	TTAA	1 cut(s)	360
Tru9I	TTAA	1 cut(s)	360
TscAI	CASTG	2 cut(s)	45, 271
TseI	GCWGC	5 cut(s)	55, 128, 694, 743, 787
TspGWI	ACGGA	1 cut(s)	770
TspMI	CCCGGG	1 cut(s)	749
TspRI	CASTG	2 cut(s)	45, 271
XceI	RCATGY	9 cut(s)	528, 560, 586, 636, 668, 684, 694, 717, 743
XhoI	CTCGAG	1 cut(s)	482
XmaI	CCCGGG	1 cut(s)	749
XmiI	GTMKAC	1 cut(s)	659
XmnI	GAANNNNTTC	1 cut(s)	180
Zsp2I	ATGCAT	5 cut(s)	526, 584, 634, 692, 741

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.