MD01G1041200.v1.1

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007378
Physical Location & Seq
Forward (+)
13822077 .. 13827727
5651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1041200.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGTTGTGCTCTTTACAAGGATCTCTCTTGACTCTCGCCATCCTCACCTTGCTCTCCTTCATCTATTTCTCCCTCAATTCCCTCCATCCCTCCGCCTCCGCCTCCCCCACCACTTACATCTCCTCCTCCGACGACGACGCAGACAATGAGCAGTTCTCCGACATCTACCACTCGCCGGAGGTGTTCCGGTTGAATTTCGCCGAGATGGAAGCCAAATTCAAGGTCTACATCTACCCGGACGGCGATCCGAACACGTTTTACCAGACGCCGAGGAAGCTCACCGGTAAGTACGCCAGCGAGGGCTATTTCTTTCAGAATATCAGAGAAAGTCACTTCCGAACTGACGATCCGGATCAGGCTCACCTCTTCTTCATCCCTATCTCCTCCCACAAGATGCGAGGCAAGGGTGCATCTTATGAAAACATGACCATAATTGTTCGGGACTATGTGGAGAGCTTGATATCCAAGTATCCTTACTGGAACCGAACCTTGGGCGCTGACCACTTCTTTGTCACGTGTCATGATGTTGGGGTGAGGGCAACTGAAGGGCTTCCACTTCTTGTGAAAAACTCAATTCGAGTTGTGTGCTCCCCTGGCTATGATGTTGGTTTCATTCCGCACAAAGATGTTGCCCTTCCTCAAGTACTTCAGCCATTTGCTCTTCCAGCCGGAGGAAACGATGTGGAGAACAGGACAACCCTCGGTTTTTGGGCCGGTCATCGGAACTCCAAAATTAGAGTTATACTGGCACGCGTATGGGAGAATGACACAGAGCTTTATATATTGAACAACAGAATAAACAGGGCAGAAGGGAATTTGTTATATCAAAGGAAGTTTTATGAAACCAAGTTCTGCATATGCCCTGGTGGCTCGCAGGTTAACAGTGCTCGCCCAACTGACGCAATCCATTATGGATGTATTCCTGTGATATTATCAAATTACTATGACCTGCCATTCAATGACATTCTCGATTGGCGAAAATTTGCTGTTATACTTAGGGAGAAGGATGTTTATCAACTTAAACAAATTCTTAAGGACATACCCTATTCAGAGTTTCTTACGCTTCACAAAAACTTGGTTAAGGTCCAGCAGCACTTCCAGTGGAATTCACCGCCTGTCAAATATGATGCATTTCACATGGTCATGTACGATCTTTGGTTGCGCCACCATGTGATCAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

395

Amino Acids

45.56

Weight (kDa)

6.94

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 70 - 346 5.1e-57 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 865, 957
AccI GTMKAC 1 cut(s) 225
AccII CGCG 1 cut(s) 753
AccIII TCCGGA 1 cut(s) 349
AciI CCGC 4 cut(s) 93, 99, 617, 1112
AclWI GGATC 4 cut(s) 28, 239, 341, 360
AcsI RAATTY 6 cut(s) 193, 215, 814, 980, 1026, 1105
AcuI CTGAAG 2 cut(s) 564, 632
AcvI CACGTG 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 266
AdeI CACNNNGTG 1 cut(s) 1171
AfaI GTAC 3 cut(s) 290, 645, 1148
AfiI CCNNNNNNNGG 4 cut(s) 175, 490, 671, 720
AflII CTTAAG 1 cut(s) 1031
AflIII ACRYGT 3 cut(s) 252, 515, 751
AgeI ACCGGT 1 cut(s) 281
AgsI TTSAA 4 cut(s) 193, 220, 787, 958
AjnI CCWGG 2 cut(s) 592, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AloI GAACNNNNNNTCC 2 cut(s) 331, 363
AluBI AGCT 3 cut(s) 277, 456, 775
AluI AGCT 3 cut(s) 277, 456, 775
Alw21I GWGCWC 3 cut(s) 11, 590, 889
AlwI GGATC 4 cut(s) 28, 239, 341, 360
Aor13HI TCCGGA 1 cut(s) 349
AoxI GGCC 1 cut(s) 711
ApeKI GCWGC 1 cut(s) 1090
ApoI RAATTY 6 cut(s) 193, 215, 814, 980, 1026, 1105
ArsI GACNNNNNNTTYG 4 cut(s) 207, 239, 491, 523
AsiGI ACCGGT 1 cut(s) 281
Asp700I GAANNNNTTC 1 cut(s) 549
AspLEI GCGC 2 cut(s) 497, 1164
AspS9I GGNCC 2 cut(s) 711, 1084
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 5 cut(s) 37, 271, 353, 544, 1101
AvaII GGWCC 1 cut(s) 1084
BbrPI CACGTG 1 cut(s) 516
Bbv12I GWGCWC 3 cut(s) 11, 590, 889
BbvI GCAGC 1 cut(s) 1102
BccI CCATC 3 cut(s) 47, 93, 199
BceAI ACGGC 1 cut(s) 256
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciT130I CCWGG 2 cut(s) 594, 864
BciVI GTATCC 1 cut(s) 480
BclI TGATCA 1 cut(s) 1173
BcnI CCSGG 1 cut(s) 236
BfoI RGCGCY 1 cut(s) 498
BfrI CTTAAG 1 cut(s) 1031
BfuAI ACCTGC 2 cut(s) 865, 957
BfuI GTATCC 1 cut(s) 480
BglI GCCNNNNNGGC 1 cut(s) 867
BisI GCNGC 1 cut(s) 1091
BlsI GCNGC 1 cut(s) 1092
BmcAI AGTACT 1 cut(s) 645
Bme1390I CCNGG 3 cut(s) 236, 594, 864
Bme18I GGWCC 1 cut(s) 1084
BmgT120I GGNCC 2 cut(s) 711, 1084
BmiI GGNNCC 1 cut(s) 482
BmrFI CCNGG 3 cut(s) 236, 594, 864
BmsI GCATC 3 cut(s) 384, 419, 1117
BplI GAGNNNNNCTC 2 cut(s) 140, 172
BpuEI CTTGAG 1 cut(s) 624
BpuMI CCSGG 1 cut(s) 236
BsaAI YACGTR 1 cut(s) 516
BsaBI GATNNNNATC 2 cut(s) 351, 1011
BsaHI GRCGYC 1 cut(s) 266
BsaJI CCNNGG 5 cut(s) 269, 489, 592, 700, 862
BsaWI WCCGGW 3 cut(s) 186, 281, 349
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 490, 671, 720
Bse118I RCCGGY 2 cut(s) 281, 713
Bse1I ACTGG 3 cut(s) 482, 750, 1099
Bse8I GATNNNNATC 2 cut(s) 351, 1011
BseAI TCCGGA 1 cut(s) 349
BseBI CCWGG 2 cut(s) 594, 864
BseDI CCNNGG 5 cut(s) 269, 489, 592, 700, 862
BseGI GGATG 5 cut(s) 39, 85, 372, 920, 1012
BseJI GATNNNNATC 2 cut(s) 351, 1011
BseLI CCNNNNNNNGG 4 cut(s) 175, 490, 671, 720
BseNI ACTGG 3 cut(s) 482, 750, 1099
BseRI GAGGAG 3 cut(s) 112, 115, 373
BseXI GCAGC 1 cut(s) 1102
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 1 cut(s) 713
BshTI ACCGGT 1 cut(s) 281
BsiHKAI GWGCWC 3 cut(s) 11, 590, 889
BsiSI CCGG 7 cut(s) 176, 187, 236, 282, 350, 669, 714
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 4 cut(s) 175, 490, 671, 720
BsmFI GGGAC 1 cut(s) 455
BsnI GGCC 1 cut(s) 713
Bsp1286I GDGCHC 3 cut(s) 11, 590, 889
Bsp13I TCCGGA 1 cut(s) 349
Bsp143I GATC 6 cut(s) 20, 244, 346, 352, 1150, 1173
BspACI CCGC 4 cut(s) 93, 99, 617, 1112
BspANI GGCC 1 cut(s) 713
BspEI TCCGGA 1 cut(s) 349
BspFNI CGCG 1 cut(s) 753
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 1 cut(s) 482
BspMI ACCTGC 2 cut(s) 865, 957
BspPI GGATC 4 cut(s) 28, 239, 341, 360
BspQI GCTCTTC 1 cut(s) 666
BspTI CTTAAG 1 cut(s) 1031
BsrFI RCCGGY 2 cut(s) 281, 713
BsrI ACTGG 3 cut(s) 482, 750, 1099
BssAI RCCGGY 2 cut(s) 281, 713
BssECI CCNNGG 5 cut(s) 269, 489, 592, 700, 862
BssMI GATC 6 cut(s) 20, 244, 346, 352, 1150, 1173
BssNI GRCGYC 1 cut(s) 266
BssT1I CCWWGG 1 cut(s) 489
Bst2UI CCWGG 2 cut(s) 594, 864
Bst4CI ACNGT 1 cut(s) 884
Bst6I CTCTTC 2 cut(s) 371, 666
BstACI GRCGYC 1 cut(s) 266
BstAFI CTTAAG 1 cut(s) 1031
BstBAI YACGTR 1 cut(s) 516
BstC8I GCNNGC 4 cut(s) 295, 751, 872, 889
BstDEI CTNAG 1 cut(s) 995
BstF5I GGATG 5 cut(s) 39, 85, 372, 920, 1012
BstFNI CGCG 1 cut(s) 753
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 2 cut(s) 497, 1164
BstKTI GATC 6 cut(s) 23, 247, 349, 355, 1153, 1176
BstMBI GATC 6 cut(s) 20, 244, 346, 352, 1150, 1173
BstMWI GCNNNNNNNGC 4 cut(s) 274, 594, 665, 867
BstNI CCWGG 2 cut(s) 594, 864
BstSCI CCNGG 3 cut(s) 234, 592, 862
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 1 cut(s) 1102
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 1 cut(s) 713
BtsCI GGATG 5 cut(s) 39, 85, 372, 920, 1012
BtsIMutI CAGTG 2 cut(s) 889, 1106
BveI ACCTGC 2 cut(s) 865, 957
Cac8I GCNNGC 4 cut(s) 295, 751, 872, 889
CciI TCATGA 1 cut(s) 520
CfoI GCGC 2 cut(s) 497, 1164
Cfr10I RCCGGY 2 cut(s) 281, 713
Cfr13I GGNCC 2 cut(s) 711, 1084
CseI GACGC 3 cut(s) 146, 274, 908
Csp6I GTAC 3 cut(s) 289, 644, 1147
CspAI ACCGGT 1 cut(s) 281
CviAII CATG 5 cut(s) 424, 521, 1138, 1144, 1169
CviQI GTAC 3 cut(s) 289, 644, 1147
DdeI CTNAG 1 cut(s) 995
DpnI GATC 6 cut(s) 22, 246, 348, 354, 1152, 1175
DpnII GATC 6 cut(s) 20, 244, 346, 352, 1150, 1173
DraIII CACNNNGTG 1 cut(s) 1171
Eam1104I CTCTTC 2 cut(s) 371, 666
EarI CTCTTC 2 cut(s) 371, 666
EciI GGCGGA 2 cut(s) 82, 88
Eco130I CCWWGG 1 cut(s) 489
Eco32I GATATC 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 1084
Eco57I CTGAAG 2 cut(s) 564, 632
Eco72I CACGTG 1 cut(s) 516
EcoRI GAATTC 1 cut(s) 1105
EcoRII CCWGG 2 cut(s) 592, 862
EcoRV GATATC 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 489
EcoT22I ATGCAT 1 cut(s) 1132
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 5 cut(s) 427, 524, 1141, 1147, 1172
FaqI GGGAC 1 cut(s) 455
FatI CATG 5 cut(s) 423, 520, 1137, 1143, 1168
FauNDI CATATG 1 cut(s) 857
FbaI TGATCA 1 cut(s) 1173
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 1091
FokI GGATG 5 cut(s) 26, 72, 359, 927, 1019
Fsp4HI GCNGC 1 cut(s) 1091
GlaI GCGC 2 cut(s) 496, 1163
GluI GCNGC 1 cut(s) 1091
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 1 cut(s) 713
HapII CCGG 7 cut(s) 176, 187, 236, 282, 350, 669, 714
HgaI GACGC 3 cut(s) 146, 274, 908
HhaI GCGC 2 cut(s) 497, 1164
Hin1I GRCGYC 1 cut(s) 266
Hin1II CATG 5 cut(s) 427, 524, 1141, 1147, 1172
Hin6I GCGC 2 cut(s) 495, 1162
HinP1I GCGC 2 cut(s) 495, 1162
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HinfI GANTC 1 cut(s) 31
HpaI GTTAAC 1 cut(s) 880
HpaII CCGG 7 cut(s) 176, 187, 236, 282, 350, 669, 714
HphI GGTGA 5 cut(s) 37, 271, 353, 544, 1101
Hpy166II GTNNAC 2 cut(s) 226, 880
Hpy188I TCNGA 8 cut(s) 130, 160, 249, 315, 323, 338, 723, 1051
Hpy188III TCNNGA 5 cut(s) 28, 350, 440, 521, 968
Hpy8I GTNNAC 2 cut(s) 226, 880
Hpy99I CGWCG 3 cut(s) 134, 137, 140
HpyAV CCTTC 5 cut(s) 67, 539, 644, 803, 997
HpyCH4III ACNGT 1 cut(s) 884
HpyCH4IV ACGT 2 cut(s) 254, 515
HpyCH4V TGCA 3 cut(s) 410, 855, 1130
HpyF10VI GCNNNNNNNGC 4 cut(s) 274, 594, 665, 867
HpyF3I CTNAG 1 cut(s) 995
HpySE526I ACGT 2 cut(s) 254, 515
Hsp92I GRCGYC 1 cut(s) 266
Hsp92II CATG 5 cut(s) 427, 524, 1141, 1147, 1172
HspAI GCGC 2 cut(s) 495, 1162
Kpn2I TCCGGA 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 1173
KspAI GTTAAC 1 cut(s) 880
Kzo9I GATC 6 cut(s) 20, 244, 346, 352, 1150, 1173
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 1 cut(s) 593
Lsp1109I GCAGC 1 cut(s) 1102
LweI GCATC 3 cut(s) 384, 419, 1117
MaeII ACGT 2 cut(s) 254, 515
MaeIII GTNAC 2 cut(s) 329, 511
MalI GATC 6 cut(s) 22, 246, 348, 354, 1152, 1175
MboI GATC 6 cut(s) 20, 244, 346, 352, 1150, 1173
MboII GAAGA 3 cut(s) 358, 361, 653
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 3 cut(s) 11, 590, 889
MluI ACGCGT 1 cut(s) 751
MlyI GAGTC 1 cut(s) 25
MmeI TCCRAC 2 cut(s) 153, 183
Mph1103I ATGCAT 1 cut(s) 1132
MroI TCCGGA 1 cut(s) 349
MroXI GAANNNNTTC 1 cut(s) 549
MseI TTAA 4 cut(s) 879, 1020, 1032, 1080
MslI CAYNNNNRTG 3 cut(s) 624, 754, 1142
MspCI CTTAAG 1 cut(s) 1031
MspI CCGG 7 cut(s) 176, 187, 236, 282, 350, 669, 714
MspR9I CCNGG 3 cut(s) 236, 594, 864
MvaI CCWGG 2 cut(s) 594, 864
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 4 cut(s) 274, 594, 665, 867
NciI CCSGG 1 cut(s) 236
NdeI CATATG 1 cut(s) 857
NdeII GATC 6 cut(s) 20, 244, 346, 352, 1150, 1173
NlaIII CATG 5 cut(s) 427, 524, 1141, 1147, 1172
NlaIV GGNNCC 1 cut(s) 482
NmeAIII GCCGAG 2 cut(s) 226, 294
NmuCI GTSAC 2 cut(s) 329, 511
NsiI ATGCAT 1 cut(s) 1132
PagI TCATGA 1 cut(s) 520
PciSI GCTCTTC 1 cut(s) 666
PdmI GAANNNNTTC 1 cut(s) 549
PinAI ACCGGT 1 cut(s) 281
PkrI GCNGC 1 cut(s) 1092
PleI GAGTC 1 cut(s) 25
PmaCI CACGTG 1 cut(s) 516
PmlI CACGTG 1 cut(s) 516
PpsI GAGTC 1 cut(s) 25
Ppu21I YACGTR 1 cut(s) 516
Psp6I CCWGG 2 cut(s) 592, 862
PspCI CACGTG 1 cut(s) 516
PspGI CCWGG 2 cut(s) 592, 862
PspN4I GGNNCC 1 cut(s) 482
PspPI GGNCC 2 cut(s) 711, 1084
PsrI GAACNNNNNNTAC 2 cut(s) 242, 274
PsuI RGATCY 1 cut(s) 20
RsaI GTAC 3 cut(s) 290, 645, 1148
RsaNI GTAC 3 cut(s) 289, 644, 1147
RseI CAYNNNNRTG 3 cut(s) 624, 754, 1142
SapI GCTCTTC 1 cut(s) 666
SaqAI TTAA 4 cut(s) 879, 1020, 1032, 1080
SatI GCNGC 1 cut(s) 1091
Sau3AI GATC 6 cut(s) 20, 244, 346, 352, 1150, 1173
Sau96I GGNCC 2 cut(s) 711, 1084
ScaI AGTACT 1 cut(s) 645
SchI GAGTC 1 cut(s) 25
ScrFI CCNGG 3 cut(s) 236, 594, 864
SduI GDGCHC 3 cut(s) 11, 590, 889
SfaNI GCATC 3 cut(s) 384, 419, 1117
SinI GGWCC 1 cut(s) 1084
SmiMI CAYNNNNRTG 3 cut(s) 624, 754, 1142
SmlI CTYRAG 2 cut(s) 639, 1031
SmoI CTYRAG 2 cut(s) 639, 1031
SsiI CCGC 4 cut(s) 93, 99, 617, 1112
StyD4I CCNGG 3 cut(s) 234, 592, 862
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 1 cut(s) 884
TaiI ACGT 2 cut(s) 257, 518
TaqI TCGA 2 cut(s) 577, 969
TatI WGTACW 1 cut(s) 643
Tru1I TTAA 4 cut(s) 879, 1020, 1032, 1080
Tru9I TTAA 4 cut(s) 879, 1020, 1032, 1080
TscAI CASTG 2 cut(s) 889, 1106
TseFI GTSAC 2 cut(s) 329, 511
TseI GCWGC 1 cut(s) 1090
Tsp45I GTSAC 2 cut(s) 329, 511
TspDTI ATGAA 5 cut(s) 49, 361, 432, 601, 855
TspRI CASTG 2 cut(s) 889, 1106
Vha464I CTTAAG 1 cut(s) 1031
VpaK11BI GGWCC 1 cut(s) 1084
XapI RAATTY 6 cut(s) 193, 215, 814, 980, 1026, 1105
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 549
ZrmI AGTACT 1 cut(s) 645
Zsp2I ATGCAT 1 cut(s) 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.