MD15G1310000.v1.1

Exostosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007378
Physical Location & Seq
Reverse (-)
31146226 .. 31149459
3234 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1310000.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGAGTGTGGGTAAGCAAAAGCAGTTCCAATTCCAATCGCACTCGCAATCACAGTCGCCGTCGTCGTCTCCTCCGATGTTGAGCTCTTTACAAGGATCTCTCCTAACTCTCGCCATCCTCACCTTGCTCTCCTTCACCTACTTCTCCCTCCATTCTCTCCATCCCTCCTCCCCCTCGCCCGCCACCGCCACCACTTACATTTCCTCCTCCTCATCCGCGGCCGACGATGAGCAGTTCTCCGACACCTACCACTCGCCGGAGGTGTTCCGGTTGAATTTCGCCGAGATGGAAGCCAAATTCAAGGTGTACATATACCCGGACGGCGATCCGAACACGTTTTACCAGACGCCGAGGAAGCTCACTGGCAAGTACGCCAGCGAGGGCTATTTCTTTCAGAATATCAGAGAGAGTCACTTCCGAACCGACGATCCGGATCAGGCTCACCTCTTCTTCATCCCTATCTCCTGCCACAAGATGCGAGGCAAGGGTACATCTTATGACAACATGACCATAATCGTTCGGGACTATGTGGAGAGCTTGATATCCAAGTATCCTTACTGGAACAGAACCTTGGGCGCGGACCACTTCTTTGTTACGTGCCACGATGTTGGGGTGAGGGCAACTGAAGGACTTCCACTTCTTGTGAAAAACTCGATTCGAGTTGTGTGCTCCCCTAGCTATGATGTTGGTTTCATTCCGCACAAAGATGTAGCCCTTCCTCAAGTACTCCAGCCATTTGCTCTTCCAGCCGGAGGAAACGATGTGGAGAACAGGACAACCCTCGGTTTTTGGGCTGGTCATCGAAATTCCAAAATTAGAGTTATACTGGCACGTGTATGGGAGAACGACACAGAGCTTTACATATTGAACAACAGAATAAATAGGGCAGAGGGGAATTTGTTATATCAAAAGAAGTTTTATGAAACCAAATTCTGCATATGCCCAGGTGGCTCCCAGGTCAACAGCGCTCGTCCAACTGACTCAATCCATTACGGTTGTATTCCTGTGATATTATCAAATTACTATGACCTGCCATTCAATGACATTCTCGATTGGCGAAAATTTGCTGTTATCCTTAGGGAGAAGGATGTGTATCAACTCAAACAAATTCTTAAGGACATACCCTATTCAGAGTTTCTTACACTTCACAAAAACTTGGTTAAGGTCCAGCAGCACTTCCAGTGGAATGCACCGCCTGTGAAATATGATGCATTTCACATGGTCATGTACGATCTCTGGTTGCGCCACCATGTGATCAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

422

Amino Acids

48.41

Weight (kDa)

8.35

Isoelectric Point (pI)

52.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 97 - 373 5.7e-57 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1038
AccII CGCG 2 cut(s) 218, 578
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 7 cut(s) 180, 186, 216, 218, 578, 698, 1193
AclWI GGATC 4 cut(s) 103, 320, 422, 441
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 7 cut(s) 274, 296, 805, 895, 929, 1061, 1107
AcuI CTGAAG 1 cut(s) 645
AcvI CACGTG 1 cut(s) 833
AcyI GRCGYC 1 cut(s) 347
AdeI CACNNNGTG 1 cut(s) 1252
AfaI GTAC 5 cut(s) 308, 371, 490, 726, 1229
AfeI AGCGCT 1 cut(s) 967
AfiI CCNNNNNNNGG 2 cut(s) 256, 752
AflII CTTAAG 1 cut(s) 1112
AflIII ACRYGT 2 cut(s) 333, 832
AgsI TTSAA 4 cut(s) 274, 301, 868, 1039
AjnI CCWGG 2 cut(s) 943, 954
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AloI GAACNNNNNNTCC 2 cut(s) 412, 444
AluBI AGCT 5 cut(s) 84, 358, 537, 678, 856
AluI AGCT 5 cut(s) 84, 358, 537, 678, 856
Alw21I GWGCWC 2 cut(s) 86, 671
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 4 cut(s) 103, 320, 422, 441
Aor13HI TCCGGA 1 cut(s) 430
Aor51HI AGCGCT 1 cut(s) 967
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 1171
ApoI RAATTY 7 cut(s) 274, 296, 805, 895, 929, 1061, 1107
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 3 cut(s) 578, 968, 1245
AspS9I GGNCC 2 cut(s) 580, 1165
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 4 cut(s) 112, 127, 434, 625
AvaII GGWCC 2 cut(s) 580, 1165
AxyI CCTNAGG 1 cut(s) 1076
BanII GRGCYC 1 cut(s) 86
BbrPI CACGTG 1 cut(s) 833
Bbv12I GWGCWC 2 cut(s) 86, 671
BbvI GCAGC 1 cut(s) 1183
BccI CCATC 3 cut(s) 122, 168, 280
BceAI ACGGC 2 cut(s) 43, 337
BcgI CGANNNNNNTGC 2 cut(s) 648, 682
BciT130I CCWGG 2 cut(s) 945, 956
BciVI GTATCC 1 cut(s) 561
BclI TGATCA 1 cut(s) 1254
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 1 cut(s) 72
BfaI CTAG 1 cut(s) 675
BfoI RGCGCY 1 cut(s) 969
BfrI CTTAAG 1 cut(s) 1112
BfuAI ACCTGC 1 cut(s) 1038
BfuI GTATCC 1 cut(s) 561
BglI GCCNNNNNGGC 1 cut(s) 948
BisI GCNGC 2 cut(s) 219, 1172
BlsI GCNGC 2 cut(s) 220, 1173
BmcAI AGTACT 1 cut(s) 726
Bme1390I CCNGG 3 cut(s) 317, 945, 956
Bme18I GGWCC 2 cut(s) 580, 1165
BmgT120I GGNCC 2 cut(s) 580, 1165
BmiI GGNNCC 1 cut(s) 952
BmrFI CCNGG 3 cut(s) 317, 945, 956
BmsI GCATC 2 cut(s) 465, 1198
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpmI CTGGAG 1 cut(s) 713
BpuEI CTTGAG 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 317
BsaAI YACGTR 2 cut(s) 597, 833
BsaBI GATNNNNATC 2 cut(s) 432, 1092
BsaHI GRCGYC 1 cut(s) 347
BsaJI CCNNGG 6 cut(s) 216, 350, 570, 781, 943, 954
BsaWI WCCGGW 2 cut(s) 267, 430
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 752
Bse1I ACTGG 4 cut(s) 367, 563, 831, 1180
Bse21I CCTNAGG 1 cut(s) 1076
Bse8I GATNNNNATC 2 cut(s) 432, 1092
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 2 cut(s) 945, 956
BseDI CCNNGG 6 cut(s) 216, 350, 570, 781, 943, 954
BseGI GGATG 5 cut(s) 114, 160, 212, 453, 1093
BseJI GATNNNNATC 2 cut(s) 432, 1092
BseLI CCNNNNNNNGG 2 cut(s) 256, 752
BseNI ACTGG 4 cut(s) 367, 563, 831, 1180
BseRI GAGGAG 4 cut(s) 60, 157, 196, 199
BseX3I CGGCCG 1 cut(s) 219
BseXI GCAGC 1 cut(s) 1183
Bsh1236I CGCG 2 cut(s) 218, 578
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 1 cut(s) 221
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 2 cut(s) 86, 671
BsiSI CCGG 5 cut(s) 257, 268, 317, 431, 750
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 2 cut(s) 256, 752
BsmAI GTCTC 1 cut(s) 72
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 1 cut(s) 536
BsmI GAATGC 1 cut(s) 1192
BsnI GGCC 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 86, 671
Bsp13I TCCGGA 1 cut(s) 430
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 6 cut(s) 95, 325, 427, 433, 1231, 1254
BspACI CCGC 7 cut(s) 180, 186, 216, 218, 578, 698, 1193
BspANI GGCC 1 cut(s) 221
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 2 cut(s) 218, 578
BspLI GGNNCC 1 cut(s) 952
BspMI ACCTGC 1 cut(s) 1038
BspPI GGATC 4 cut(s) 103, 320, 422, 441
BspQI GCTCTTC 1 cut(s) 747
BspTI CTTAAG 1 cut(s) 1112
BsrGI TGTACA 1 cut(s) 306
BsrI ACTGG 4 cut(s) 367, 563, 831, 1180
BssECI CCNNGG 6 cut(s) 216, 350, 570, 781, 943, 954
BssMI GATC 6 cut(s) 95, 325, 427, 433, 1231, 1254
BssNI GRCGYC 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 2 cut(s) 945, 956
Bst4CI ACNGT 2 cut(s) 54, 995
Bst6I CTCTTC 2 cut(s) 452, 747
BstACI GRCGYC 1 cut(s) 347
BstAFI CTTAAG 1 cut(s) 1112
BstAUI TGTACA 1 cut(s) 306
BstBAI YACGTR 2 cut(s) 597, 833
BstC8I GCNNGC 2 cut(s) 180, 376
BstDEI CTNAG 1 cut(s) 1076
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 5 cut(s) 114, 160, 212, 453, 1093
BstFNI CGCG 2 cut(s) 218, 578
BstH2I RGCGCY 1 cut(s) 969
BstHHI GCGC 3 cut(s) 578, 968, 1245
BstKTI GATC 6 cut(s) 98, 328, 430, 436, 1234, 1257
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 6 cut(s) 95, 325, 427, 433, 1231, 1254
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 4 cut(s) 355, 675, 746, 948
BstNI CCWGG 2 cut(s) 945, 956
BstSCI CCNGG 3 cut(s) 315, 943, 954
BstUI CGCG 2 cut(s) 218, 578
BstV1I GCAGC 1 cut(s) 1183
BstX2I RGATCY 1 cut(s) 95
BstXI CCANNNNNNTGG 1 cut(s) 608
BstYI RGATCY 1 cut(s) 95
BstZI CGGCCG 1 cut(s) 219
Bsu36I CCTNAGG 1 cut(s) 1076
BsuI GTATCC 1 cut(s) 561
BsuRI GGCC 1 cut(s) 221
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 5 cut(s) 114, 160, 212, 453, 1093
BtsIMutI CAGTG 2 cut(s) 360, 1187
BveI ACCTGC 1 cut(s) 1038
Cac8I GCNNGC 2 cut(s) 180, 376
CfoI GCGC 3 cut(s) 578, 968, 1245
Cfr13I GGNCC 2 cut(s) 580, 1165
Cfr42I CCGCGG 1 cut(s) 219
CseI GACGC 1 cut(s) 355
Csp6I GTAC 5 cut(s) 307, 370, 489, 725, 1228
CviAII CATG 4 cut(s) 505, 1219, 1225, 1250
CviQI GTAC 5 cut(s) 307, 370, 489, 725, 1228
DdeI CTNAG 1 cut(s) 1076
DpnI GATC 6 cut(s) 97, 327, 429, 435, 1233, 1256
DpnII GATC 6 cut(s) 95, 325, 427, 433, 1231, 1254
DraIII CACNNNGTG 1 cut(s) 1252
EaeI YGGCCR 1 cut(s) 219
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 452, 747
EarI CTCTTC 2 cut(s) 452, 747
Ecl136II GAGCTC 1 cut(s) 84
EclXI CGGCCG 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 543
Eco47I GGWCC 2 cut(s) 580, 1165
Eco47III AGCGCT 1 cut(s) 967
Eco52I CGGCCG 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 645
Eco72I CACGTG 1 cut(s) 833
Eco81I CCTNAGG 1 cut(s) 1076
EcoICRI GAGCTC 1 cut(s) 84
EcoRII CCWGG 2 cut(s) 943, 954
EcoRV GATATC 1 cut(s) 543
EcoT14I CCWWGG 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 1213
EcoT38I GRGCYC 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 570
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 4 cut(s) 508, 1222, 1228, 1253
FaqI GGGAC 1 cut(s) 536
FatI CATG 4 cut(s) 504, 1218, 1224, 1249
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 1 cut(s) 938
FbaI TGATCA 1 cut(s) 1254
Fnu4HI GCNGC 2 cut(s) 219, 1172
FokI GGATG 5 cut(s) 101, 147, 199, 440, 1100
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 2 cut(s) 219, 1172
FspBI CTAG 1 cut(s) 675
GlaI GCGC 3 cut(s) 577, 967, 1244
GluI GCNGC 2 cut(s) 219, 1172
GsuI CTGGAG 1 cut(s) 713
HaeII RGCGCY 1 cut(s) 969
HaeIII GGCC 1 cut(s) 221
HapII CCGG 5 cut(s) 257, 268, 317, 431, 750
HgaI GACGC 1 cut(s) 355
HhaI GCGC 3 cut(s) 578, 968, 1245
Hin1I GRCGYC 1 cut(s) 347
Hin1II CATG 4 cut(s) 508, 1222, 1228, 1253
Hin6I GCGC 3 cut(s) 576, 966, 1243
HinP1I GCGC 3 cut(s) 576, 966, 1243
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HinfI GANTC 3 cut(s) 409, 655, 980
HpaII CCGG 5 cut(s) 257, 268, 317, 431, 750
HphI GGTGA 4 cut(s) 112, 127, 434, 625
Hpy166II GTNNAC 2 cut(s) 307, 961
Hpy188I TCNGA 7 cut(s) 75, 241, 330, 396, 404, 419, 1132
Hpy188III TCNNGA 3 cut(s) 431, 521, 1049
Hpy8I GTNNAC 2 cut(s) 307, 961
Hpy99I CGWCG 4 cut(s) 64, 67, 227, 428
HpyAV CCTTC 4 cut(s) 142, 620, 725, 1078
HpyCH4III ACNGT 2 cut(s) 54, 995
HpyCH4IV ACGT 3 cut(s) 335, 596, 832
HpyCH4V TGCA 3 cut(s) 936, 1190, 1211
HpyF10VI GCNNNNNNNGC 4 cut(s) 355, 675, 746, 948
HpyF3I CTNAG 1 cut(s) 1076
HpySE526I ACGT 3 cut(s) 335, 596, 832
Hsp92I GRCGYC 1 cut(s) 347
Hsp92II CATG 4 cut(s) 508, 1222, 1228, 1253
HspAI GCGC 3 cut(s) 576, 966, 1243
Kpn2I TCCGGA 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 1254
KspI CCGCGG 1 cut(s) 219
Kzo9I GATC 6 cut(s) 95, 325, 427, 433, 1231, 1254
LguI GCTCTTC 1 cut(s) 747
LmnI GCTCC 2 cut(s) 674, 956
Lsp1109I GCAGC 1 cut(s) 1183
LweI GCATC 2 cut(s) 465, 1198
MaeI CTAG 1 cut(s) 675
MaeII ACGT 3 cut(s) 335, 596, 832
MaeIII GTNAC 2 cut(s) 410, 592
MalI GATC 6 cut(s) 97, 327, 429, 435, 1233, 1256
MboI GATC 6 cut(s) 95, 325, 427, 433, 1231, 1254
MboII GAAGA 3 cut(s) 439, 442, 734
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 2 cut(s) 86, 671
MlyI GAGTC 2 cut(s) 418, 974
MmeI TCCRAC 2 cut(s) 264, 998
Mph1103I ATGCAT 1 cut(s) 1213
MroI TCCGGA 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 630
MseI TTAA 2 cut(s) 1113, 1161
MslI CAYNNNNRTG 3 cut(s) 705, 835, 1223
MspA1I CMGCKG 1 cut(s) 218
MspCI CTTAAG 1 cut(s) 1112
MspI CCGG 5 cut(s) 257, 268, 317, 431, 750
MspR9I CCNGG 3 cut(s) 317, 945, 956
Mva1269I GAATGC 1 cut(s) 1192
MvaI CCWGG 2 cut(s) 945, 956
MvnI CGCG 2 cut(s) 218, 578
MwoI GCNNNNNNNGC 4 cut(s) 355, 675, 746, 948
NciI CCSGG 1 cut(s) 317
NdeI CATATG 1 cut(s) 938
NdeII GATC 6 cut(s) 95, 325, 427, 433, 1231, 1254
NlaIII CATG 4 cut(s) 508, 1222, 1228, 1253
NlaIV GGNNCC 1 cut(s) 952
NmeAIII GCCGAG 2 cut(s) 307, 375
NmuCI GTSAC 1 cut(s) 410
NsiI ATGCAT 1 cut(s) 1213
PciSI GCTCTTC 1 cut(s) 747
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 71
PctI GAATGC 1 cut(s) 1192
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 1 cut(s) 655
PkrI GCNGC 2 cut(s) 220, 1173
PleI GAGTC 2 cut(s) 417, 974
PmaCI CACGTG 1 cut(s) 833
PmlI CACGTG 1 cut(s) 833
PpsI GAGTC 2 cut(s) 417, 974
Ppu21I YACGTR 2 cut(s) 597, 833
Psp124BI GAGCTC 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 943, 954
PspCI CACGTG 1 cut(s) 833
PspGI CCWGG 2 cut(s) 943, 954
PspN4I GGNNCC 1 cut(s) 952
PspPI GGNCC 2 cut(s) 580, 1165
PsrI GAACNNNNNNTAC 2 cut(s) 323, 355
PsuI RGATCY 1 cut(s) 95
RsaI GTAC 5 cut(s) 308, 371, 490, 726, 1229
RsaNI GTAC 5 cut(s) 307, 370, 489, 725, 1228
RseI CAYNNNNRTG 3 cut(s) 705, 835, 1223
SacI GAGCTC 1 cut(s) 86
SacII CCGCGG 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 747
SaqAI TTAA 2 cut(s) 1113, 1161
SatI GCNGC 2 cut(s) 219, 1172
Sau3AI GATC 6 cut(s) 95, 325, 427, 433, 1231, 1254
Sau96I GGNCC 2 cut(s) 580, 1165
ScaI AGTACT 1 cut(s) 726
SchI GAGTC 2 cut(s) 418, 974
ScrFI CCNGG 3 cut(s) 317, 945, 956
SduI GDGCHC 2 cut(s) 86, 671
SfaNI GCATC 2 cut(s) 465, 1198
Sfr303I CCGCGG 1 cut(s) 219
SgrBI CCGCGG 1 cut(s) 219
SinI GGWCC 2 cut(s) 580, 1165
SmiMI CAYNNNNRTG 3 cut(s) 705, 835, 1223
SmlI CTYRAG 2 cut(s) 720, 1112
SmoI CTYRAG 2 cut(s) 720, 1112
SsiI CCGC 7 cut(s) 180, 186, 216, 218, 578, 698, 1193
SspMI CTAG 1 cut(s) 675
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 3 cut(s) 315, 943, 954
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 2 cut(s) 54, 995
TaiI ACGT 3 cut(s) 338, 599, 835
TaqI TCGA 4 cut(s) 653, 658, 802, 1050
TatI WGTACW 2 cut(s) 306, 724
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 655
Tru1I TTAA 2 cut(s) 1113, 1161
Tru9I TTAA 2 cut(s) 1113, 1161
TscAI CASTG 2 cut(s) 367, 1187
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 1 cut(s) 1171
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 3 cut(s) 442, 682, 936
TspRI CASTG 2 cut(s) 367, 1187
Vha464I CTTAAG 1 cut(s) 1112
VpaK11BI GGWCC 2 cut(s) 580, 1165
XapI RAATTY 7 cut(s) 274, 296, 805, 895, 929, 1061, 1107
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 1 cut(s) 675
ZrmI AGTACT 1 cut(s) 726
Zsp2I ATGCAT 1 cut(s) 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.